• Title/Summary/Keyword: physiological and biochemical characteristics

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Bacterial Soft Rot of Radishby Erwinia chrysanthemi (Erwinia chrysanthemi에 의한 무 세균성 무름병)

  • 박덕환;서상태;이흥구;최국선;임춘근
    • Plant Disease and Agriculture
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    • v.5 no.1
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    • pp.61-63
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    • 1999
  • Bacterial soft rot was observed on radish grown in Hongcheon, Kanwon-Do, Korea. The soft rot symptoms began as small water-soaked lesions. The water-soaked lesions enlarged rapidly in roots and produced a foul odor. When roots were affected in the field, the shoots also became infected and watery, causing infected plants to wilt, disorganize, and die. The causal organism was isolated from the lesions, and the identified as Erwinia chrysanthemi based on the morphological, physiological and biochemical characteristics. E. chrysanthemi is first described bacterium which causes bacterial soft rot on radish in Korea.

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Screening of Giberella sp. from the Korean Paddy Field for the roduction of Gibberellic Acid and its Cultural Properties (Gibberellic acid를 생산하는 분리주 Gibberella sp. 의 배양학적 특성)

  • 오영준
    • Microbiology and Biotechnology Letters
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    • v.23 no.2
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    • pp.119-122
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    • 1995
  • A different form from Gibberella fujikuroi was isolated from the paddy field of Naju area. The strain, designated as Y107, was identified as Gibberrella sp. based on its morphological, physiological, and biochemical characteristics. The highest production of Gibberellic acid by the strain was achieved in a fermentation medium containing corn starch, glucose, soybean oil, soybean meal, NH$_{4}$NO$_{3}$, K$_{2}$HPO$_{4}$, MgSO$_{4}$, and trace elements.

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Black Leg of Potato Plants by Erwinia carotovora subsp. atroseptica (Erwinia carotovora subsp. atroseptica에 의한 감자 흑각병)

  • 박덕환;김준섭;이흥구;함영일;임춘근
    • Plant Disease and Agriculture
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    • v.5 no.1
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    • pp.64-66
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    • 1999
  • Black leg occurred in potato (Solanum tuberosum L.) grown in Pyungchang, Kangwon-Do, Korea. The symptoms began as small water-soaked lesions on stem, and the affected stems discolored black to brown. It became yellow under dry condition. When lower parts of potato were affected in the field, wilting of leaves and desiccation of the stem were developed. The causal organism was isolated from lesions and identified as Erwinia carotovora subsp. atroseptica based on the morphological, physiological and biochemical characteristics. E. carotovora subsp. atroseptica is the first described bacterium which causes black leg in potato in Korea.

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Identification of the Actinomycetes Strain No.1372, A Producer of Actinomycin $X_2$ (Actinomycin $X_2$를 생산하는 방선균 분리주 No.1372의 동정)

  • 하상철;홍순덕
    • Microbiology and Biotechnology Letters
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    • v.22 no.2
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    • pp.164-168
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    • 1994
  • Identification of the Actinomycetes isolate strain No. 1372, a producer of Actinomycin X$_{2}$ was performed by using ISP method.l The strain, designated as No. 1372, was identified as Streptomyces floridae based on its morphological, physiological and biochemical characteristics. The highest production of the antibiotics by the strain was achieved in a fermentation medium containing soluble starch, yeast extract, (NH$_{4}$)SO$_{4}$, K$_{2}$HPO$_{4}$, NaCl$_{2}$, CaCO$_{3}$, and trace element.

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단백질분해효소를 생성분비하는 호알칼리성 Coryneform bacterium TU-19의 분리 및 동정

  • 최명철;양재섭;강선철
    • Microbiology and Biotechnology Letters
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    • v.24 no.2
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    • pp.160-165
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    • 1996
  • An alkalophilic bacterium producing alkaline protease(s) was isolated from soil. It was a Gram-positive, non-sporulating, immotile, irregular rod, strictly aerobic, and weak acid-forming bacterium. The morphological, physiological, and biochemical characteristics of the isolate resembled those of the Coryneform bacteria. However, there was not any species within this genera to which this microorganism can be closely matched. Therefore, it is provisionally identified as a Coryneform bacterium TU-19.

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Some Properties of Acetobacter sp. Isolated from Traditional Fermented Vinegar (전통발효 식초에서 분리한 Agdohader sp.의 특성)

  • 박종필;김성준
    • KSBB Journal
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    • v.8 no.4
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    • pp.397-404
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    • 1993
  • Two strains were isolated from the vinegar of Korean traditional fermented rice wine and the vine gar of fermented persimmon, respectively. These strains, designated as KM and BPV, were identified as the genus Acetobacter with respect to morphological, physiological, and biochemical characteristics. The Isolates oxidized ethanol to acetate and over-oxidized acetate or lactate to CO2 and H2O. They were positive in catalase test, while being negative in oxidase, gelatin liquefaction, VP test, H2O production and indole formation tests. No ${\gamma}$-pyrones ware produced from glucose and fructose. KM was tolerant of 11% ethanol while BPV was relatively sensitive to ethanol at a higher concentration than 5%. The guanine-plus-cytosine contents of the DNA of KM and BPV strains were 53.8 and 56.6 mol%, respectively. The cellular fatty acid compositions contained in these isolates were saturated straightchain C14:0 and C16:0,, and unsaturated straight-chain C18:1. Major ubiquinone system of KM was Q-9, but that of BPV was Q-10. In morphophysiological and biochemical aspects, KM strain was similar to Acetobacter pasteurianus. However, BPV strain was different from other Acetobacter type strains.

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Biochemical and Molecular Insights into Bile Salt Hydrolase in the Gastrointestinal Microflora - A Review -

  • Kim, Geun-Bae;Lee, Byong H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.10
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    • pp.1505-1512
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    • 2005
  • Bile salt deconjugation is the most biologically significant reaction among the bacterial alterations of bile acids in the gastrointestinal tract of human and animal. The responsible enzyme, bile salt hydrolase (BSH), catalyzes the hydrolysis of glycineand/or taurine-conjugated bile salts into amino acid residues and deconjugated bile acids. Herein we review current knowledge on the distribution of BSH activity among various microorganisms with respect to their biochemical and molecular characteristics. The proposed physiological impact of BSH activity on the host animal as well as on the BSH-producing bacterial cells is discussed. BSH activity of the probiotic strains is examined on the basis of BSH hypothesis, which was proposed to explain cholesterol-lowering effects of probiotics. Finally, the potential applications of BSH research are briefly discussed.

Programmed Cell Death in Bacterial Community: Mechanisms of Action, Causes and Consequences

  • Lee, Heejeong;Lee, Dong Gun
    • Journal of Microbiology and Biotechnology
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    • v.29 no.7
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    • pp.1014-1021
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    • 2019
  • In the bacterial community, unicellular organisms act together as a multicellular being. Bacteria interact within the community and programmed cell death (PCD) in prokaryotes is a sort of altruistic action that enables the whole population to thrive. Genetically, encoded cell death pathways are triggered by DNA damage or nutrient starvation. Given the environmental and bacterial diversity, different PCD mechanisms are operated. Still, their biochemical and physiological aspects remain unrevealed. There are three main pathways; thymineless death, apoptosis-like death, and toxin-antitoxin systems. The discovery of PCD in bacteria has revealed the possibility of developing new antibiotics. In this review, the molecular and physiological characteristics of the three types of PCD and their development potential as antibacterial agents are addressed.

Characterization of airag collected in Ulaanbaatar, Mongolia with emphasis on isolated lactic acid bacteria

  • Choi, Suk-Ho
    • Journal of Animal Science and Technology
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    • v.58 no.3
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    • pp.10.1-10.10
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    • 2016
  • Background: Airag, alcoholic sour-tasting beverage, has been traditionally prepared by Mongolian nomads who naturally ferment fresh mares' milk. Biochemical and microbiological compositions of airag samples collected in Ulaanbaatar, Mongolia and physiological characteristics of isolated lactic acid bacteria were investigated. Methods: Protein composition and biochemical composition were determined using sodium dodecyl sulfate-gel electrophoresis and high performance liquid chromatography, respectively. Lactic acid bacteria were identified based on nucleotide sequence of 16S rRNA gene. Carbohydrate fermentation, acid survival, bile resistance and acid production in skim milk culture were determined. Results: Equine whey proteins were present in airag samples more than caseins. The airag samples contained 0.10-3.36 % lactose, 1.44-2.33 % ethyl alcohol, 1.08-1.62 % lactic acid and 0.12-0.22 % acetic acid. Lactobacillus (L.) helveticus were major lactic acid bacteria consisting of 9 isolates among total 18 isolates of lactic acid bacteria. L. helveticus survived strongly in PBS, pH 3.0 but did not grow in MRS broth containing 0.1 % oxgall. A couple of L. helveticus isolates lowered pH of skim milk culture to less than 4.0 and produced acid up to more than 1.0 %. Conclusion: Highly variable biochemical compositions of the airag samples indicated inconsistent quality due to natural fermentation. Airag with low lactose content should be favorable for nutrition, considering that mares' milk with high lactose content has strong laxative effect. The isolates of L. helveticus which produced acid actively in skim milk culture might have a major role in production of airag.

Silkworm-food plant-interaction: search for an alternate food plant for tasar silkworm (Antheraea mylitta Drury) rearing

  • Deka, Manabendra;Gargi, Gargi;Kumar, Rajendra;Yadav, Harendra;Sahay, Alok
    • International Journal of Industrial Entomology and Biomaterials
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    • v.30 no.2
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    • pp.58-63
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    • 2015
  • An experimental rearing of tropical tasar silkworm, Antheraea mylitta Drury was conducted to study silkworm-food plant-interaction and thereby to search for an alternate silkworm food plant. The silkworm-food-plant-interaction was studied with six different food plant species viz. Terminalia tomentosa, Terminalia arjuna, Terminalia belerica, Terminalia chebula of Combretaceae family and Lagerstroemia speciosa, Lagerstroemia parviflora of Lythraceae family. The rearing performance of silkworm on Lagerstroemia speciosa in terms of cocoons per DFL and silk ratio was found comparable with Terminalia tomentosa and Termonalia arjuna, the primary tasar silkworm food plant species. These three plant species also possessed better results in terms of physiological (leaf moisture content and net photosynthesis rate) and biochemical (Chlorophyll, protein, carbohydrate and crude fibre contents) characteristics to support silkworm rearing than Terminalia belerica, Terminalia chebula and Lagerstroemia parviflora. The correlation study between silkworm rearing performance and food plant's constituents indicates commercial perspective of Lagerstroemia speciosa as an alternate food plant for tasar silkworm rearing.