• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2021년도 학술발표회
• /
• pp.89-89
• /
• 2021

부영양화된 호수나 유속이 느린 하천에서 발생하는 녹조의 과도한 발생은 하천 생태계 훼손, 동식물의 건강, 담수의 오염 등 환경 사회 경제적으로 큰 피해를 준다. 현재 수질 측정망은 정해진 지점에서 Chlorophyll-a(Chl-a), Phycocyanin(PC)을 대표농도로 산정하고 조류경보에 활용하고 있으나, 일주일에 한번씩 샘플링을 통해 Chl-a 및 PC를 측정하여 시공간적인 신뢰성의 문제가 제기될 수 있다. 본 연구에서는 기존 점단위 조류 모니터링의 한계점을 개선하기 위해 초분광영상 자료를 머신러닝 기법에 적용하여 Chl-a 및 PC 산정 알고리즘을 개발하였다. 이를 위해 Chl-a와 PC의 최대 흡수, 반사 파장대, 주요 물 흡수 파장대 자료를 조합하여 9개의 파장비를 구축하였으며, 기존 연구에서 활용한 머신러닝 기법인 Partial Least Square, Random Forest, Gradient Boosting, Support Vector Machine, K-Nearest Neighbor, Artificial Neural Network를 검토하여 최적 모델을 선정하였다. 학습된 머신러닝의 성능을 R², NSE, RMSE 목적함수를 이용해 평가하였으며, 그 결과 ANN이 각각 PC 0.801, 0.755, 11.774 mg/m³, Chl-a 0.733, 0.622, 8.736 mg/m³로 가장 우수한 성능을 보였다. 최적화 된 ANN 모델을 백제보 상류 2016-2017년 항공 초분광영상에 적용하여 시공간에 따른 조류 분포변화를 평가하고자 한다.

• 생태와환경
• /
• 제36권4호통권105호
• /
• pp.413-419
• /
• 2003

1999년과 2001턴, 여름부터 가을까지 대청호의 남조류 수화현상에 대한 강우영향을 조사하였다. 두 해의 6,7월 강수량은 매우 유사하였으나, 수화발생 시기인 8,9월의 강수량은 현저한 차이를 보였다. 1999년에는 8월 하순 이후 그전 2개월에 비해 약 70%의 강수량 증가가 있었으나, 2001년에는 같은 기간 동안 5분의 1수준으로 감소하였다. 더 높은 엽록소-a, 파이코시아닌, 남조류 농도가 2001년 측정되었으며, 이것은 극심한 수화의 발생으로 이어졌다. 또한 2001년에는 조사기간동안 남조류의 비율이 항상 80%를 상회하였으며, 남조류는 Microcystis spp.가 대부분을 차지하였다. 이와는 반대로 1999년에는 수화의 발생이 보고되지 않았다. 이러한 차이에도 불구하고 수화가 절정을 이루는 시기는 두 해 모두 8월 하순에서 9월 초순으로, 강수량에 관계없이 일정하였다. 수화 이전 시기의 적당한 강수와 이후의 충분한 일조 시기가 남조류의 수화 형성에 영향을 끼치는 중요인자들 중의 하나로 판단되었다.

• Bulletin of the Korean Chemical Society
• /
• 제33권3호
• /
• pp.933-940
• /
• 2012

Recently, it has been shown that quantum coherence appears in energy transfers of various photosynthetic lightharvesting complexes at from cryogenic to even room temperatures. Because the photosynthetic systems are inherently complex, these findings have subsequently interested many researchers in the field of both experiment and theory. From the theoretical part, simplified dynamics or semiclassical approaches have been widely used. In these approaches, the quantum-classical Liouville equation (QCLE) is the fundamental starting point. Toward the semiclassical scheme, approximations are needed to simplify the equations of motion of various degrees of freedom. Here, we have adopted the Poisson bracket mapping equation (PBME) as an approximate form of QCLE and applied it to find the time evolution of the excitation in a photosynthetic complex from marine algae. The benefit of using PBME is its similarity to conventional Hamiltonian dynamics. Through this, we confirmed the coherent population transfer behaviors in short time domain as previously reported with a more accurate but more time-consuming iterative linearized density matrix approach. However, we find that the site populations do not behave according to the Boltzmann law in the long time limit. We also test the effect of adding spurious high frequency vibrations to the spectral density of the bath, and find that their existence does not alter the dynamics to any significant extent as long as the associated reorganization energy is changed not too drastically. This suggests that adopting classical trajectory based ensembles in semiclassical simulations should not influence the coherence dynamics in any practical manner, even though the classical trajectories often yield spurious high frequency vibrational features in the spectral density.

• 동아시아식생활학회지
• /
• 제21권1호
• /
• pp.31-37
• /
• 2011

This study was conducted to investigate the quality characteristics of bread prepared with spirulina powder (0, 0.4, 0.8, and 1.2%). The volume and specific volume of bread decreased with increasing spirulina powder content. The pH of the bread with 1.2% spirulina powder was the highest. Content of phycocyanin increased with addition of spirulina powder. For color values, increasing spirulina powder content resulted in decreased lightness, redness, and yellowness in the crust. Amylogram showed that spirulina addition increased initial gelatinization temperature, decreased breakdown, and lowered the setback and consistency, which prevented retrogradation. Moreover, the hardness of spirulina bread decreased as spirulina addition increased. Further, springiness, cohesiveness, gumminess, chewiness. and resilience of bread with 0.8% spirulina powder were the lowest. Sensory results showed that the scores of overall preference and buying intention in the bread with 0.8% spirulina were the highest among the control and other groups.

• 한국환경과학회지
• /
• 제27권1호
• /
• pp.27-38
• /
• 2018

This study was carried out in the Bukhan River in the summer of 2014 and 2015, to identify the relationship between geosmin and the morphological changes in Anabaena. Identification of Anabaena was conducted using morphological and molecular analyses. Anabaena in this study was similar to Anabaena circinalis, A. crass, and A. spiroides with regard to regular coils, vegetative cell, akinete shape, and size, hoever, it was distinguishabl from A. crass and A. spiroides because of its larger trichome coil size. Additionally, the sequences of phycocyanin (PC) gene from Anabaena showed a 99% genetic similarity with A. circinalis NIES-1647 strain. The coil diameter of trichome ranged from 106 to $899{\mu}m$, and the diameter and abundance showed an insignificant positive correlation (r=0.544, p<0.05). The result of relationship between the coil diameter and the cell number per 360-degree rotation was kept at $33.8{\pm}5.2$ cells per $100{\mu}m$ diameter despite variable diameter. The average geosmin concentrations in 2014 and 2015 were investigated to be 99 ng/L and 35 ng/L, respectively. A. circinalis cell density contributed considerably to the change in geosmin and was positively correlated with geosmin concentration (2014; r=0.599, p<0.01, 2015; r=0.559, p<0.01). Our results suggest that geosmin and coil diameter could be estimated with the help of cell density.

• 한국식품과학회지
• /
• 제52권5호
• /
• pp.487-494
• /
• 2020

본 연구는 비타민 및 무기질 등의 영양성분을 고루 함유하고 있을 뿐만 아니라 특히 식이섬유와 수용성 산성 다당류인 포피란이 풍부한 김 분말을 항산화성을 증진시킬 목적으로 크리스피 쌀 과자에 시리얼의 0, 1, 3, 5% 비율로 첨가하여 품질특성과 항산화능을 분석하였다. 환원당은 마른 김을 첨가함에 따라 크리스피 쌀 과자 중의 함량이 감소했다. 색도는 마른 김의 첨가량에 의해 명도와 적색도, 황색도 모두 유의적으로 감소하였다. 산화 방지 등의 항산화 활성을 가지는 색소 성분인 피코시아닌과 클로로필 함량 모두 마른 김 첨가량에 따라 유의적으로 증가했다. 항산화능은 총 페놀 함량, 플라보노이드 함량과 DPPH 라디칼 소거능 및 hydroxyl 라디컬 소거능 모두 마른 김 분말의 농도에 비례하여 우수해지는 것으로 나타났다. 감각평가 결과, 전반적인 만족감은 LP5 첨가군이 유의적으로 가장 높았고 대조군의 점수가 가장 낮았다. 크리스피 쌀 과자에 마른 김 분말을 첨가하는 것은 항산화능 증진에도 도움을 주어 크리스피 쌀 과자의 상품으로서의 가치를 높일 수 있을 것으로 사료된다.

• Journal of Microbiology and Biotechnology
• /
• 제20권7호
• /
• pp.1134-1139
• /
• 2010

This study explored the chronic effects of different doses of the triazole fungicide tebuconazole on the growth, and metabolic and enzymatic functions of the filamentous paddy field cyanobacterium, Westiellopsis prolifica Janet. The growth of the cyanobacterium was determined by an estimation of the change in pigment contents. Chlorophyll-a, carotenoids, and accessory pigments such as phycocyanin, allophycocyanin, and phycoerythrin were shown to decline over a 16-day period by a factor of 92%, 93%, 83%, 95%, and 100%, respectively, with increasing doses of the fungicide. Metabolic and enzymatic activities were also adversely affected. Over the 16 days, a gradual rise in total phenol content was recorded when Westiellopsis prolifica Janet was treated with 60 ppm of the fungicide, despite the reduction in carbohydrates, proteins, and amino acids by 96%, 92%, and 90%, respectively. Moreover, the enzymes nitrate reductase (NR), glutamine synthetase (GS), and succinate dehydrogenase (SDH) also registered reductions of 93%, 90%, and 98%, respectively. This study indicates that tebuconazole, although an important fungicide used extensively in rice fields, exhibits an inhibitory effect on the growth and metabolic activities of Westiellopsis prolifica Janet and hence possibly on other varieties as well.

• Journal of Microbiology and Biotechnology
• /
• 제23권6호
• /
• pp.739-749
• /
• 2013

The seasonal and spatial diversity of picocyanobacteria (Pcy) in lakes of the Great Mazurian Lakes (GLM) system was examined by DGGE analysis of molecular markers derived from the 16S-23S internal transcribed spacer (ITS) of the ribosomal operon and the phycocyanin operon (cpcBA-IGS). The study of nine lakes, ranging from mesotrophy to hypereutrophy, demonstrated seasonal variance of Pcy. The richness and Shannon diversity index calculated on the basis of both markers were higher in spring and lower in early and late summer. No statistically significant relationships were found between the markers and trophic status of the studied lakes or Pcy abundance. There were, however, statistically significant relationships between the diversity indices and sampling time. The analysis pointed to a different distribution of the two markers. The ITS marker exhibited more unique sequences in time and space, whereas a greater role for common and ubiquitous sequences was indicated by the cpcBA-IGS data. Examination of the Pcy community structure demonstrated that communities were grouped in highly similar clusters according to sampling season/time rather than to the trophic status of the lake. Our results suggest that time is more important than trophic status in shaping the diversity and structure of Pcy communities. The seasonal changes in picocyanobacteria and differences in diversity and community structures are discussed in the context of well-established ecological hypotheses: the PEG model, intermediate disturbance hypothesis (IDH), and horizontal gene transfer (HGT).

• 환경생물
• /
• 제38권3호
• /
• pp.424-432
• /
• 2020

The present study aimed to analyze the metaproteome of the microbial community comprising harmful algal bloom (HAB) in the Daechung reservoir, Korea. HAB samples located at GPS coordinates of 36°29'N latitude and 127°28'E longitude were harvested in October 2013. Microscopic observation of the HAB samples revealed red signals that were presumably caused by the autofluorescence of chlorophyll and phycocyanin in viable cyanobacteria. Metaproteomic analysis was performed by a gelbased shotgun proteomic method. Protein identification was conducted through a two-step analysis including a forward search strategy (FSS) (random search with the National Center for Biotechnology Information (NCBI), Cyanobase, and Phytozome), and a subsequent reverse search strategy (RSS) (additional Cyanobase search with a decoy database). The total number of proteins identified by the two-step analysis (FSS and RSS) was 1.8-fold higher than that by one-step analysis (FSS only). A total of 194 proteins were assigned to 12 cyanobacterial species (99 mol%) and one green algae species (1 mol%). Among the species identified, the toxic microcystin-producing Microcystis aeruginosa NIES-843 (62.3%) species was the most dominant. The largest functional category was proteins belonging to the energy category (39%), followed by metabolism (15%), and translation (12%). This study will be a good reference for monitoring ecological variations at the meta-protein level of aquatic microalgae for understanding HAB.

• 동아시아식생활학회지
• /
• 제20권5호
• /
• pp.769-775
• /
• 2010

Quality characteristics and antioxidant activity of spirulina with added tofu were investigated in the presence of different coagulants ($CaSO_4$, $MgCl_2$, Glucono delta lactone (GDL), vinegar). The yield of the spirulina tofu was the highest when coagulated with GDL. The pH of spirulina tofu with $MgCl_2$ was the highest compared to those of other coagulants. The tumidity of spirulina tofu was the highest with $MgCl_2$. In the color analysis of spirulina tofu, the L (lightness) value was the highest with $CaSO_4$, whereas a (redness) value was the highest with $MgCl_2$. C-phycocyanin contents were the highest with the $CaSO_4$. Phycoerythrin of spirulina tofu for vinegar was 0.194 mg/mL, and GDL content was 0.214 mg/mL. Additionally, the antioxidant activity of spirulina tofu was the highest with $MgCl_2$ $IC_{50}$ values of the DPPH radical scavenging activity of spirulina tofu with $MgCl_2$ was 89.2 mg/g, GDL 93.5 mg/mL, $CaSO_4$ 116.7 mg/mL, and vinegar 118.0 mg/mL. The texture analyses (TPA) results showed that hardness was in the order of vinegar>$MgCl_2$>GDL>$CaSO_4$. For the overall acceptability of the preference test, the spirulina tofu that was made using $MgCl_2$ coagulant had the highest score. Based on these results, it is suggested that $MgCl_2$ is the proper coagulant for the preparation of spirulina with added tofu.