• Title/Summary/Keyword: photosynthetic unit

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Purification and Characterization of Cytochrome c Oxidase from Photosynthetic Bacterium, Rhodopseudomonas gelatinosa (광합성세균 Rhodopseudomonas gelatinosa 의 시토크롬 c 산화효소의 정제 및 특성)

  • 강대길;최원기
    • Korean Journal of Microbiology
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    • v.30 no.2
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    • pp.101-107
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    • 1992
  • Cytochrome c oxida5e from chemotrophically grown R p , geliitinosu was purified by cytochrome c affinity chromatography and DEAE-Sephacel ion exchange chromatography. The molecular weight of the cytochrome c oxidase was approximately 110.000 Da by sephacryl s-300 gel chromatography and approximately 52, 000 Da by SDS-gel electrophoresis, respectively. Therefore. cytochrolne c oxidase of Rps. gehtinosu seems to be dimer. The cytochrome c oxidasc was very sensitive to temperature. It's Km and Vmax were 20 pM and 44 unitlmg protein for horsc heart cytochrome c as a substrate. respectively, and its optimum pH and temperature were 6.4 and 25$^{\circ}$C. respectively. The absorption peaks of the reduced cytochrome c oxidase showed at 554 nm, 523 nm. and 422 nm. The activiiy of cytochrome c oxidase was inhibited by KCN, and NaN3, but not by CO, antimycir~ A. and myxothiazol. The cytochrome c-551 was produced either in phototrophically or chemotrophically grown Rps. gelaiinosci. The rcduced cytochrome c-551 was oxidized by b-type cytochrome c oxidase from Rp.v. gc.lrtino.sc~. Km and Vmax of cytochrome c oxidase was 26 pM and 31 unitlnlg protein For cytochrome c-551 as a substrate. respectively. Thercfore. thc electron transfer chain of chemotrophically grown Rps. glatinosa seems lo be ubiquinol cytochrome bc, complex -'cytochrome c-55lMb-type cytochrome c oxidase+02.

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Growth, Yield and Photosynthesis of Introduced Kenaf Cultivars in Korea (신도입 케나프 품종의 파종시기에 빠른 생육 및 수량 변동과 광합성 특성)

  • 강시용;김판기;강영길;강봉균;유장걸;류기중;송희섭
    • Korean Journal of Plant Resources
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    • v.17 no.2
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    • pp.139-146
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    • 2004
  • Kenaf (Hibiscus cannabinus L.), an annual plant of the family Malvaceae, is considered to be the most promising for alternative plants with potential use as a non-wood fiber source. The objectives of this study were to select the optimum seeding date and adaptable cultivar among newly introduced kenaf cultivars (Everglades-41, Tainung-2 and Chingpi-3) in Jeju island, and to clarify the photosynthetic characteristics of those cultivars. Among the three cultivars, the fresh shoot weight per unit area of Chingpi-3 at harvest season showed highest through all seeding dates, that of while Everglades-41 was the lowest. The difference of shoot yield at harvest mainly due to seedling stand rate and plant number per unit area among the cultivars. The Chingpi-3 showed the highest shoot fresh weight in the seeding date of May 11 and decreased trend in the late seeding dates. Net photosynthesis rate was higher on Everglades-41 with entire type leaf than other two cultivars with palmate type leaf. The activity of ribulose-1,5-bisphosphate carboxylase/foxygenase (rubisco) estimated from the A-Ci curve showed highest in Chingpi-3 among three cultivars. These results suggest that Chingpi-3 might be adaptable cultivar with seeding date of around May 10 for kenaf production in Jeiu island.

Effects of Water Table Depth in Different Soil Texture on Growth and Yield of Barley and Wheat (토성별 지하수위가 밀, 보리의 생육 및 수량에 미치는 영향)

  • 이홍석;박의호;송현숙;구자환
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.2
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    • pp.195-202
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    • 1995
  • This experiment was performed to characterize the optimum water table level for the growth and yield of barley(var. Olbori) and wheat(var, Grumil), Olbori and Grumil were grown in the 550 liter plastic pot filled with silt loam or sandy loam, During the whole growth period, the water table adjusted to be 20, 30, 40, 50, and 70cm, Higher water table was resulted in the decrease in plant height and top dry weight, but in the increase of the ratio of top to root dry weight, especially in barley, This suggested that high water table level affected more the growth of top than that of root, The number and area of green leaves were decreased as the water table was higher than 30 to 40cm at the late growth period(May 18, 1993), The largest number and area of green leaves were shown at 50cm of water table in sandy loam and at 70cm in silt loam, As the water table was high, the leaf chlorophyll content was low, And barley was affected more significantly than wheat by soil texture, The photosynthetic activity was decreased remarkably at 20cm water table, Heading period was 2 to 3 and 4 days earlier at the 20cm water table of sandy loam in barley and wheat, respectively, However this earlier heading was not shown in silt loam, Grain filling was accelerated 5 to 7 days earlier in barley and 10 days in wheat grown at 20cm water table, The highest yield was present at 50 and 70cm water table, The yield was decreased remarkably at 20cm water table, resulting that yield reduction ratio of barley was 71.1% and 72, 2%, and that of wheat was 41.0% and 60, 0% in sandy loam and silt loam, respectively, High water table decreased the number of spike per unit area, but increased the seed weight per spike in barley, However, High water table reduced the seed weight per spike in wheat. There was significant correlation between yield and leaf chlorophyll content in wheat and barley, Yield was correlated significantly with green leaf area in barley, and with top dry weight, ratio of top to root dry weight chlorophyll content and photosynthetic activity in wheat. The optimum water table was 50 to 70cm in wheat and barley, They grew fairly well at 30cm water table of sandy loam, and at 40cm of silt loam.

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Changes in Sink capacity and Source Activity of Rice Cultivars in Response to Shift of Heading date (벼 품종들의 출수기에 따른 동화산물 생산능력 및 수용기관 크기 변화)

  • Lee, Sok-Young;Kwon, Yong-Woong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.2
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    • pp.260-267
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    • 1995
  • In temperate zone planting rice at different date subjects the Crop to different climatic condition. The present study aimed at comparison of the change in source-sink relationship of the Japonica(J) and that of IndicaxJaponica(I$\times$J) type rice cultivars caused by shift of heading date. Two J- and two I$\times$J-type cultivars were made to head on August 16, August 26, and September 5. Sink capacity was changed by shift of heading date in different mode between the types of cultivars. In both types major determinant of sink capacity was number of effective tillers, and the number of spikelets per panicle was the minor. In J-type earlier planting/heading was beneficial to increased panicle numbers and this was due mainly to a larger diurnal difference in temperature. I$\times$J-type cultivars favored a higher daily mean temperature to increase the sink capacity. The ability of source at heading, in terms of leaf area per panicle, chlorophyll content per spiklet, photosynthetic ability of leaves per unit area at 25$\^{\circ}C$, carbohydrate and N contents of leaves, was not so different among different heading dates in both types. However, the source activity was governed principally by temperature during grain filling. The J-type cultivars headed on Sept. 5 and I$\times$J-type cultivars headed later than August 16 could not have had sufficient source activity in grain filling due to lower temperature.

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The Effect of UV-B Irradiation and Far-red Light Reduction on the Growth of Laurus nobilis in Indoor (실내환경에서 자외선 조사 및 원적외선 차단이 월계수의 생장에 미치는 영향)

  • Fujiwara, Keisuke;Toda, Hiroto;Choi, Dong-Su
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.5
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    • pp.1-7
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    • 2012
  • The main purpose of this research is to evaluate the effect of light quality on the growth and physiological activities of Laurus nobilis plants growing indoors, the L. nobilis seedlings were grown under four types of lighting for 180 days. The seedlings were grown under 4 different treatments((n=9 seedlings per treatment): control, control supplemental UV-B irradiated(+UV), FR reduced(-FR) and simultaneously supplemental UV-B irradiated and FR reduced(+UV-FR)). It was found that UV-B irradiation(+UV) reduced dry weight and leaf area, and increased leaf thickness and the amount of UV-absorbing compounds per unit leaf area. In contrast, a reduction in far-red(FR) light did not affect any of these parameters. Interestingly, however, the elongation growth and net photosynthetic rate of the L. nobilis seedlings grown under simultaneous UV-B irradiation and FR reduction(+UV-FR) were significantly decreased than the control treatment. From these results, it is concluded that the light quality has a large effect on the indoor growth of L. nobilis. This study can suggest basic information for managed in the L. nobilis in indoor using light quality.

The Photosynthetic Character and Yield of Three Naked Barley Cultivars (Hordeum vulgare L.) Affected by Different Levels of Nitrogen Application (질소시용량의 차이가 과맥의 광합성 관련형질과 수량에 미치는 영향)

  • Kim, S.H.;Cho, J.Y.;Kwon, H.J.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.2
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    • pp.157-174
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    • 1984
  • Four different levels of nitrogen were applied to 3 naked barley cultivars (Hordeum vulgare L.) Yonezawa, Baekdong and Yeongsanbori. Changes in chlorophyll content, green leaf area and light penetration were monitored after heading. Yield and its components were examined upon these changes. Increased level of N-application delayed the days to heading and ripening by 2-3 and 6-7 days, respectively, in Yonezawa. Yonezawa with a short culm was more resistant to lodging than two tall ones (Baekdong, Yeongsanbori) under heavy nitrogen applications. Yield was affected mostly by the number of spikes per unit area and 1, 000 grain weight at all levels of N-applications. The increase in dry weight of Baekdong was more than in that of Yonezawa. A sharp increase in dry weight was observed after 3 weeks of heading. Maximum yield was obtained at $N_2$ in Yonezawa (234kg/10a) and Baekdong (302kg/10a) and at $N_1$ in Yeongsanbori (332kg/10a). It appears essential to attain the sufficient leaf area and the increase in chlorophyll content for maximum yield. However, too much leaf area at the highest N level up to the ripening stage was not beneficial for dry matter production.

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Treatment of Food Garbage Using a Treatment Reactor and Microbial Consortium (발효소멸기를 이용한 음식물 쓰레기의 감량 및 악취제거)

  • Koh, Rae-Hyun;Lee, Kang-Hyoung;Yoo, Jin-Soo;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.42 no.4
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    • pp.306-312
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    • 2006
  • Disposal of food garbage in most large cities is very troublesome task. To date, microbiological treatment has been received an attention as a garbage decomposition process. In this study, the inoculation effect of some cellulase, amylase and protease-producing bacteria and photosynthetic bacteria on food garbage treatment was examined. They were added into a treatment reactor specially designed in this study together with food garbage and incubated in various conditions for 15 days and the removals of food garbage and foul smell produced during the treatment were analyzed. Average decomposition percentages of the inoculated food garbage in treatment reactor were 11 and 18.8% under intermittent aeration (once in a day) and continuous aeration conditions (2 L/min), respectively, and these were higher than removal percentages in the corresponding uninoculated reactors,3.4 and 13.8%. Optimal pH and temperature for food garbage decomposition by inoculated bacteria were pH 7.0 and $30^{\circ}C$. Maximal decomposition percentage in the inoculated food garbage was 35% under the optimal condition (pH 7, $30^{\circ}C$, and continuous aeration). The malodor compounds generated from food garbage treatment such as complex foul smell and sulfur compounds were effectively reduced about 84% and 25.5%, respectively, with a biofilter composed of purple nonsulfur bacteria trapped in sponge. This decomposing capability of food garbage by these bacteria can be utilized for the rapid and efficient treatment of food garbage.

Effects of Nitrogen Level and Defoliation at Maturing Stage on the Yield Components of Rice (질소시용량(窒素施用量) 및 등숙기(登熟期) 절엽처리(切葉處理)가 수도수량구성요소(水稻收量構成要素)에 미치는 영향(影響))

  • Ahn, Su-Bong;Lee, Jong-Chul
    • Korean Journal of Agricultural Science
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    • v.4 no.1
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    • pp.26-33
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    • 1977
  • We have studied the responce of nitrogen on the newly bred varieties, Yusin and Milyang #23, and the effects of defoliation at maturing stage on the rate of ripened grains under different nitrogen levels. The results obtained are summarized as follows : 1. Milyang #23 as well as Tongil was found to be highly nitrogen responsive variety and showed good grain maturing percentage. The number of grain per unit area affected the total yield under the high levels of nitrogen application. The variety, Yusin, was less responsive to nitrogen and had also less leaf area than other varieties under the high level of nitrogen application. Yusin also seemed to have low maturing percentage due to unbalanced ratio between photosynthetic area and size of storage. 2. The low internodes and leaf blade of Yusin and Milyang #15 were grown too much under high levels of nitrogen and these caused more lodging and less light penetration in pant canopy. 3. The effects of defoliation at maturing stage on yield was high under the high levels of nitrogen application, especially when defoliation was done early stage of maturing. The effects of defoliation appeared to be greates in Tongil than in Milyang #15.

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Seed Germination, Efficiency of Photosynthesis and Proper Covering Materials for Wintering in Amorphophallus konjac K. (구약감자 품종(品種)들의 종자발아력(種子發芽力), 광합성(光合成) 능력(能力)의 차이(差異)와 안전(安全) 월동(越冬)을 위한 피복재료선발(被覆材料選拔))

  • Lee, Hee-Duck;Ju, Jung-Il;Choi, Chang-Yeol;Lee, Jung-Il
    • Korean Journal of Medicinal Crop Science
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    • v.2 no.1
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    • pp.14-19
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    • 1994
  • Elephant food(Amorphophallus konjac K.) have been utilized its tubers in workedmaterials for a health and diet food. The author supposed that it was increased the area of cultivation and demand. This experiments were conducted to select the proper covering material during winter in order to increase yield of tubers and decrease input by 2 year's continuous cultivation, also to verified ability of seed germination and to measured efficiency of photosynthesis of plant. The proper covering materials for wintering were rice straw and rice hull. These materials were covered at 5 cm thick and at field was promoted according to emergence appearing after winter. The yields were 5,790kg /10a at 4,730kg /10a, respectively. Yield increase was 120% and 80% than that of control. The seeds collected at August 22 were germinated about 84 percent, and it was not necessary to treatment of low temperature or germination-accelerated chemicals. The widest leaf area was ranged $1,218-1,438cm^2$ at October 20 and was varied. The efficiency of photosynthesis was highest at 65-95 days after leaf emergence. The line of broad leaf and high photosynthetic efficiency per unit area was greater compare with yield. Therefore, it was supposed that these characteristics will use a marker for selection for high-yielding lines.

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Studies on the Varietal Difference in the Physiology of Ripening in Rice with Special Reference to Raising the Percentage of Ripened Grains (수도 등숙의 품종간차이와 그 향상에 관한 연구)

  • Su-Bong Ahn
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.14
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    • pp.1-40
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    • 1973
  • There is a general tendency to increase nitrogen level in rice production to insure an increased yield. On the other hand, percentage of ripened grains is getting decreased with such an increased fertilizer level. Decreasing of the percentage is one of the important yield limiting factors. Especially the newly developed rice variety, 'Tongil' is characterized by a relatively low percentage of ripened grains as compared with the other leading varieties. Therefore, these studies were aimed to finding out of some measures for the improvement of ripening in rice. The studies had been carried out in the field and in the phytotron during the period of three years from 1970 to 1972 at the Crop Experiment Station in Suwon. The results obtained from the experiments could be summarized as follows: 1. The spikelet of Tongil was longer in length, more narrow in width, thinner in thickness, smaller in the volume of grains and lighter in grain weight than those of Jinheung. The specific gravity of grain was closely correlated with grain weight and the relationship with thickness, width and length was getting smaller in Jinheung. On the other hand, Tongil showed a different pattern from Jinheung. The relationship of the specific gravity with grain weight was the greatest and followed by that with the width, thickness and length, in order. 2. The distribution of grain weight selected by specific gravity was different from one variety to another. Most of grains of Jinheung were distributed over the specific gravity of 1.12 with its peak at 1.18, but many of grains of Tongil were distributed below 1.12 with its peak at 1.16. The brown/rough rice ratio was sharply declined below the specific gravity of 1.06 in Jinheung, but that of Tongil was not declined from the 1.20 to the 0.96. Accordingly, it seemed to be unfair to make the specific gravity criterion for ripened grains at 1.06 in the Tongil variety. 3. The increasing tendency of grain weight after flowering was different depending on varieties. Generally speaking, rice varieties originated from cold area showed a slow grain weight increase while Tongil was rapid except at lower temperature in late ripening stage. 4. In the late-tillered culms or weak culms, the number of spikelets was small and the percentage of ripened grains was low. Tongil produced more late-tillered culms and had a longer flowering duration especially at lower temperature, resulting in a lower percentage of ripened grains. 5. The leaf blade of Tongil was short, broad and errect, having light receiving status for photosynthesis was better. The photosynthetic activity of Tongil per unit leaf area was higher than that of Jinheung at higher temperature, but lower at lower temperature. 6. Tongil was highly resistant to lodging because of short culm length, and thick lower-internodes. Before flowering, Tongil had a relatively higher amount of sugars, phosphate, silicate, calcium, manganese and magnesium. 7. The number of spikelets of Tongil was much more than that of Jinheung. The negative correlation was observed between the number of spikelets and percentage of ripened grains in Jinheung, but no correlation was found in Tongil grown at higher temperature. Therefore, grain yield was increased with increased number of spikelets in Tongil. Anthesis was not occurred below 21$^{\circ}C$ in Tongil, so sterile spikelets were increased at lower temperature during flowering stage. 8. The root distribution of Jinheung was deeper than that of Tongil. The root activity of Tongil evaluated by $\alpha$-naphthylamine oxidation method, was higher than that of Jinheung at higher temperature, but lower at lower temperature. It is seemed to be related with discoloration of leaf blades. 9. Tongil had a better light receiving status for photosynthesis and a better productive structure with balance between photosynthesis and respiration, so it is seemed that tongil has more ideal plant type for getting of a higher grain yield as compared with Jinheung. 10. Solar radiation during the 10 days before to 30 days after flowering seemed enough for ripening in suwon, but the air temperature dropped down below 22$^{\circ}C$ beyond August 25. Therefore, it was believed that air temperature is one of ripening limiting factors in this case. 11. The optimum temperature for ripening in Jinheung was relatively lower than that of Tongil requriing more than $25^{\circ}C$. Air temperature below 21$^{\circ}C$ was one of limiting factors for ripening in Tongil. 12. It seemed that Jinheung has relatively high photosensitivity and moderate thermosensitivity, while Tongil has a low photosensitivity, high thermosensitivity and longer basic vegetative phase. 13. Under a condition of higher nitrogen application at late growing stage, the grain yield of Jinheung was increased with improvement of percentage of ripened grains, while grain yield of Tongil decreased due to decreasing the number of spikelets although photosynthetic activity after flowering was. increased. 14. The grain yield of Jinheung was decreased slightly in the late transplanting culture since its photosynthetic activity was relatively high at lower temperature, but that of Tonil was decreased due to its inactive photosynthetic activity at lower temperature. The highest yield of Tongil was obtained in the early transplanting culture. 15. Tongil was adapted to a higher fertilizer and dense transplanting, and the percentage of ripened grains was improved by shortening of the flowering duration with increased number of seedlings per hill. 16. The percentage of vigorous tillers was increased with a denser transplanting and increasing in number of seedlings per hill. 17. The possibility to improve percentage of ripened grains was shown with phosphate application at lower temperature. The above mentioned results are again summarized below. The Japonica type leading varieties should be flowered before August 20 to insure a satisfactory ripening of grains. Nitrogen applied should not be more than 7.5kg/10a as the basal-dressing and the remained nitrogen should be applied at the later growing stage to increase their photosynthetic activity. The morphological and physiological characteristics of Tongil, a semi-dwarf, Indica $\times$ Japonica hybrid variety, are very different from those of other leading rice varieties, requring changes in seed selection by specific gravity method, in milling and in the cultural practices. Considering the peculiar distribution of grains selected by the method and the brown/rough rice ratio, the specific gravity criterion for seed selection should be changed from the currently employed 1.06 to about 0.96 for Tongil. In milling process, it would be advisable to bear in mind the specific traits of Tongil grain appearance. Tongil is a variety with many weak tillers and under lower temperature condition flowering is delayed. Such characteristics result in inactivation of roots and leaf blades which affects substantially lowering of the percentage of ripened grains due to increased unfertilized spikelets. In addition, Tongil is adapted well to higher nitrogen application. Therefore, it would be recommended to transplant Tongil variety earlier in season under the condition of higer nitrogen, phosphate and silicate. A dense planting-space with three vigorous seedlings per hill should be practiced in this case. In order to manifest fully the capability of Tongil, several aspects such as the varietal improvement, culural practices and milling process should be more intensively considered in the future.he future.

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