• 한국패류학회지
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• 제29권4호
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• pp.291-301
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• 2013

This study researched and compared the sedimentation environment and photosynthetic pigments at Gomso (GS) -bay, Yoja (YJ) -bay and KangJin (KJ) -bay in May, 2012. It was shown that KJ-bay consist of C (clay) and M (mud), and GS-bay consists of Zs (silty sand) and Sz (sandy silt). Averagely, IL of YJ-bay was 4.98%, KJ-bay 6.10%, and GS-bay 1.45%. As for COD concentration, there were no places that exceeded Japanese sediment contamination standard 20 mg/g-dry. As for AVS concentration, in case of KJ-bay, two places exceeded Japanese sediment contamination standard 0.2 mg/g-dry. If we look into the average C/N ratio of bays, YJ-bay showed 8.50, KJ-bay 6.60, and GS-bay 5.52, thus all of them showed the characteristic of oceanic origin. As for the plankton make-up classified by photosynthetic pigments, diatom was dominant, and both ratios of pigment and C/chlorophyll. a showed the relatively lower distribution at GS-bay than at KJ-bay and YJ-bay. It is judged that this is related to the characteristic of flow-in, and it implies that predation process and decomposition by organic matters actively occurs at YJ-bay and KJ-bay.

• 한국환경과학회지
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• 제1권1호
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• pp.1.1-11
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• 1992

To find heavy metal-specific effects on the photosynthetic apparatus of higher plants, we investigated effects of $CuCl_2$, HgCl_2$ and $ZnCl_2$ on electron transport activity and chlorophyll fluorescence induction kinetics of chloroplasts isolated from barley seedlings. Effects on some related processes such as germination, growth and photosynthetic pigments of the test plants were also studied. Germination and growth rate were inhibited in a concentration-dependent manner by these metals. Mercury was shown to be the most potent inhibitor of germination, growth and biosynthesis of photosynthetic pigments of barley plants. In the inhibition of electron transport activity, quantum yield of PS II, and chlorophyll fluorescence induction kinetics of chloroplasts isolated from barley seedlings, mercury chloride showed more pronounced effects than other two metals. Contrary to the effects of other two metals, mercury chloride increased variable fluorescence significantly and abolished qE in the fluorescence induction kinetics from broken chloroplasts of barley seedlings. This increase in variable fluorescence is due to the inhibition of the electron transport chain after PS ll and the following dark reactions. The inhibition of qE could be attributed to the interruption of pH formation and do-epoxidation of violaxathin to zeaxanthin in thylakoids by mercury. This unique effect of mercury on chlorophyll fluorescence induction pattern could be used as a good indicator for testing the presence and/or the concentration of mercury in the samples contaminated with heavy metals.

• Journal of Ecology and Environment
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• 제29권1호
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• pp.41-47
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• 2006

We investigated the enect of $O_3$ on the photosynthetic characteristics of tree of heaven (Ailanthus altissima) that is naturalized plant and used as restoration plant for contaminated area. Two-year-old seedlings were planted to pots and transferred into closed $O_3$ chamber. Photosynthetic pigments contents and photosynthetic characteristics were measured every three weeks under 100 pub $O_3$ fumigation. There was no visible foliar injury by $O_3$ exposure and contents of photosynthetic pigments did not show significant differences between control and $O_3$-treated seedlings. Also there were no significant differences in stomatal conductance, and water use efficiency. But photosynthetic rate and apparent quantum yield (AQY) of $O_3$ treated seedlings were reduced after nine weeks of ozone fumigation. In addition, the reduction of carboxylation efficiency and photorespiration were observed in the leave of $O_3$ treated seedlings after six weeks. In accordance with our result, carbon fixation system of A. altissima was most sensitive to $O_3$ stress to evaluate physiological damage induced by $O_3$.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1211-1216
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• 2004

A MNNG (N-methyl-N'-nitro-N-nitrosoguanidine) induced chromium resistant strain ($Cr^{r}18$) of unicellular cyanobacterium Anacystis nidulans has been isolated and characterized. The resistant strain could grow (although restricted to $50\%$ of control) in chromium concentration (180${\mu}M$) lethal to the wild-type. Sublethal ($160{\mu}M$) concentration of $Cr^{6+}$ significantly reduced (13-$37.5$) all the photosynthetic pigments of A. nidulans with maximum reduction in phycoerythrin followed by ChI $\alpha$. Pigments of A. nidulans were drastically decreased in lethal concentration of Cr^{6+} with maximum reduction in phycoerythrin ($75\%$) and allophycocyanin ($67.5\%$). Resistant strain $Cr^{r}18$ resisted toxic effects of sublethal and lethal concentrations of $Cr^{6+}$ on photosynthetic pigments as revealed by less decrease in pigments as compared to A. nidulans. Effect of $Cr^{6+}$ stress was also studied on nitrogen assimilation and phosphate uptake. Sublethal concentration of $Cr^{6+}$ drastically reduced ($71.5\%$) nitrate uptake by A. nidulans while a decrease of $29\%$ was observed in strain $Cr^{r}18$. Short (2 day) exposure of A. nidulans and its resistant strain $Cr^{r}18\;to\;Cr^{6+}$ did not affect nitrate reductase and glutamine synthetase (transferase), whereas longer (10 day) exposure to $Cr^{6+}$ lowered activities of both enzymes in A. nidulans but not significantly in the strain $Cr^{r}18$. Ammonium uptake by both strains was not affected by $Cr^{6+}$. Thus, $Cr^{6+}$ affected photosynthetic pigments, nitrogen assimilation, and phosphate uptake of A. nidulans, while strain $Cr^{r}18$ was able to resist toxic effects of the metal. Advantages of using strain $Cr^{r}18$ for bioremediation purposes have been evaluated by studying $Cr^{6+}$ removal from the solution. Resistant strain $Cr^{r}18$ was able to remove $33\%$ more $Cr^{6+}$ than A. nidulans and thus it can prove to be a good candidate for bioremediation of $Cr^{6+}$ from polluted waters.

• 환경생물
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• 제28권1호
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• pp.20-29
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• 2010

Cochlodinium polykrikoides has occurs regularly during the summer in the South Sea of Korea. To investigate photosynthetic pigments concerned with phytoplankton community structure as bloom of Cochlodinium polykrikoides, the experiment was sampled at 20 stations three times between July and September 2002 in the Southern Sea of Korea. The distribution of peridinin, the biomarker of dinoflagellate, was higher at the blooming time than it was before and it disappeared after bloom. The correlative coefficients between Chl. a and peridinin at the blooming time and out of bloom were 0.9253 and 0.1613, respectively. This result indicated that the bloom was caused by dinoflagellate. The correlative coefficients between Chl. a and fucoxanthin were 0.3282 and 0.9759, respectively, and the correlative coefficients showed the succession from dinoflagellate to diatom. This result means that the bloom of Cochlodinium polykrikoides can be detected by Chl. a information from satellite remote sensing. Therefore, if the algorithm to detect peridinin in addition to Chl. a were to be developed, dinoflagellate red tide could be monitored more effectively.

• ALGAE
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• 제18권1호
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• pp.83-88
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• 2003

• 한국해양학회지:바다
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• 제20권2호
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• pp.71-77
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• 2015

High-Performance Liquid Chromatography (HPLC) 분석방법은 식물플랑크톤의 생물량 및 일차생산력을 추정하기 위한 지시자로서 chlorophyll a 농도를 측정하고 carotenoids의 종류를 파악해 종조성을 확인하기 위해 널리 이용되고 있다. 그러나 대량시료의 분석에 요구되는 시료 전처리 과정 중 여과지 분쇄는 상당한 시간과 숙련이 요구된다. 본 연구에서는 엽록소 및 carotenoids의 정량분석에 대한 여과지 분쇄의 영향을 파악하고자 동해 남서부 해역의 시료를 이용해 여과지 분쇄 전후의 광합성 색소 농도를 비교 평가했다. HPLC 분석에서 여과지 분쇄 생략 시 Chl a의 경우 평균 45% 과소평가되었다. 또한 pico, nano 크기 식물플랑크톤의 지표색소인 Zeaxanthin, 19'-butanoyloxyfucoxanthin, 19'-hexanoyloxyfucoxanthin는 최대 77~85% 과소평가되었다. 크기가 작은 식물플랑크톤의 경우 여과지 분쇄가 생략될 경우 불완전한 추출로 지표색소의 농도가 실제보다 저평가될 가능성이 크다는 것을 확인하였다. 따라서 HPLC 분석에서 여과지 분쇄 생략 시 Chl a 뿐 아니라 carotenoids 또한 과소평가 되므로 모든 경우에서 여과지 분쇄 과정이 반드시 필요하다고 판단된다.

• 아시안잔디학회지
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• 제11권1호
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• pp.45-58
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• 1997

Pigments and thylakoid membrane proteins were investigated in wild type and PS I- mutants from Synechocystis sp. PCC6803 Comparing morphological features, B2 was less fluorescent than the other strains. The contents of chlorophyll a were propotional to the FNR activity in thylakoid membrane. The FNR activity of mutants was lower than that of wild type. In the result of pigments analysis, mutants had smaller cholophyll a than that of wild type. The major carotenoid was found to he $\beta$-caroene, but aeaxanthin was barely detected in thylakoid membrane of mutants. The polypeptide, 14.8kD was detected by electrophoresis in mutants. It was considered to be the modification of 15.4kD in wild type. Membrane polypeptides of 17.6 and 19.7kD were not detected in mutants. In the result of western blotting, subunit I was detected in all strains, but subunit II was barely detected in mutants. Subunit II was not detected in B2 at all. In view of the results so far achieved, the changes of contents of chlorophyll and zeaxanthin were affected by the defficiency or modification of functional domain in subunit I. Also the modification in subunit I affected the subunit II- binding site in PS I. As the result, efficiency of photosynthesis was decreased. Key words: Synechoystis sp. PCC6803, PS I - mutant, Photosynthetic efficiency, Pigment,Thylakoid membrane proteins, Subunit I, II.

• Journal of Ginseng Research
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• 제32권4호
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• pp.347-356
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• 2008

Forest-grown American ginseng (Panax quinquefolius L.) is exposed to daily and seasonal light variations. Our goal was to determine the effect of understory light changes on the maximum quantum yield of photosystem II, expressed as $F_v/F_m$, and photosynthetic pigment composition of two-year-old plants. Understory light photon flux density and sunfleck durations were characterized using hemispherical canopy photography. Our results showed that understory light significantly affected the $F_v/F_m$ of American ginseng, especially during the initial development of the plants when light levels were the highest, averaging 28 mol $m^{-2}d^{-1}$. Associated with low $F_v/F_m$ during its initial development, American ginseng had the lowest levels of epoxidation state of the xanthophyll cycle of the season, suggesting an active dissipation of excess light energy absorbed by the chlorophyll pigments. As photon flux density decreased after the deployment of the forest canopy to less than 10 mol $m^{-2}d^{-1}$, chlorophyll a/b decreased suggesting a greater investment in light harvesting pigments to reaction centers in order to absorb the fleeting light energy.

• Environmental Engineering Research
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• 제20권1호
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• pp.41-50
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• 2015

The cyanobacterium Anabaena doliolum Ind1 isolated from a coal mining site was tested for removal of cadmium at optimum pH 7.0 and temperature $25^{\circ}C$. The organism recorded high percentage of metal removal (92-69%) within seven days of exposure to 0.5-2.0 ppm cadmium. Biosorption onto the cell surface was the primary mode of metal removal. Fourier transform infrared spectroscopy (FTIR) established hydroxyl, amides, carboxyl, sulphate and carbonyl groups to be the major functional groups on the cell surface involved in cadmium binding. Cellular ultrastructure and a range of vital physiological processes (i.e., photosynthetic pigments, respiration, photosynthesis, heterocyst frequency and nitrogenase activity) remained unaffected upon 0.5 ppm treatment; higher concentrations of cadmium exerted visible adverse effects. Amongst the five photosynthetic pigments tested, phycocyanin was the most targeted pigment (inhibition was 15-89%). Both respiration and photosynthetic activities were inhibited by cadmium with more severe effect seen on respiration. 2.0 ppm cadmium exposure also had drastic negative effect on nitrogenase activity (87% decreased).