• Journal of Plant Biology
• /
• v.17 no.3
• /
• pp.113-117
• /
• 1974

In counting chromosome number and karyotype study, it is necessary to let chromosomes on metaphase by pretreatment before fixation. For this purpose, colchicine, or 8-oxyquinoline are generally used. The author found out that chromosomes could be scattered by illuminating cyanoferrate complex solution in which root-tips were sunk. As materials, 8 sorts of plant such as Allium fisturosum, allium tuberosum Rottler, Triticum vulgare were used. Their root-tips were sunk on the bottom of beaker in potassium ferricyanide solution $3{\times}10-4M$ and illuminated through the solution by sterilizing lamp for 1~2 hours in dark room, keeping 10 cm distance from light source to the surface of solution and 2cm depth of solution. Then again, they were illuminated to the light which was somewhat weaker intensity than the former (distance, 16cm; depth, 3cm) for 1.5~2 hours after immersed in 1/100N-HCl and washed in water for each 5minutes. By such methods chromosomes could be scattered. About the mechanism of scattering, it is supposed that CN and Fe(CN)x ions $(x {\leq}5)$ which were gradually produced in the process of photodissociation acted together on the scattering of chromosomes.

• Bulletin of the Korean Chemical Society
• /
• v.31 no.1
• /
• pp.92-99
• /
• 2010

Photoexcitation of a precursor ion inside a cell floated at high voltage installed in a tandem time-of-flight (TOF) mass spectrometer provides triple tandem mass spectrometric information and allows kinetic and mechanistic studies. In this work, the factors affecting, or downgrading, the performance of the technique were identified. Ion-optical and computational analyses showed that an optimum instrument could be designed by utilizing a reflectron with linear-plus-quadratic potential inside. Theoretical predictions were confirmed by tests with instruments built with different ion-optical layout. With optimized instruments, masses of intermediate ions in the consecutive dissociation of a precursor ion could be determined with the maximum error of $\pm5$ Da. We also observed excellent agreement in dynamical parameters (critical energy and entropy) for the dissociation of a model peptide ion determined by instruments with different ion-optical layout operated under optimum conditions. This suggests that these parameters can be determined reliably by the kinetic method developed previously when properly designed and operated tandem TOF instruments are used.

• Bulletin of the Korean Chemical Society
• /
• v.33 no.6
• /
• pp.1998-2004
• /
• 2012

We carried out DFT calculations for monohydrated sulfuric and phosphoric acids. We are interested in clusters which differ in orientation of hydrogen atoms only. Such molecular complexes are close in energy, since they lie in the vicinity of the global minimum energy structure on the flat potential energy surface. For monohydrated sulfuric acid we identified four different isomers. The monohydrated phosphoric acid forms five different conformers. These systems are difficult to study from the theoretical point of view, since binding energy differences in several cases are very small. For each structure, we calculated harmonic vibrational frequencies to be sure that if the optimized structures are at the local or global minima on the potential energy surface. The analysis of calculated -OH vibrational frequencies is useful in interpretation of infrared photodissociation spectroscopy experiments. We employed four different DFT functionals in our calculations. For each structure, we calculated binding energies, thermodynamic properties, and harmonic vibrational frequencies. Our analysis clearly shows that DFT approach is suitable for studying monohydrated inorganic acids with different hydrogen atom orientations. We carried out MP2 calculations with aug-cc-pVDZ basis set for both monohydrated acids. MP2 results serve as a benchmark for DFT calculations.

• Bulletin of the Korean Chemical Society
• /
• v.30 no.4
• /
• pp.913-916
• /
• 2009

Femtosecond vibrational spectroscopy was used to probe the dynamics of CO rebinding to hemoglobin (Hb), denatured by 4.0 M GdnHCl in $D_2O# at 283 K, after photolysis of HbCO. The stretching mode of $^{13}CO$ bound to the denatured $Hb^{13}CO$ showed a single band centered at 1922 $cm^{-1}$, indistinguishable from that of denatured $Mb^{13}CO$. Geminate rebinding of CO to the denatured Hb was accelerated more than 1000 times, suggesting that the native structure of the Hb is required to suppress efficient geminate rebinding of CO, as is the case in Mb. The geminate yield and rate for CO rebinding are almost the same in both the denatured Hb and Mb. Similarity in the equilibrium spectrum and rebinding dynamics of CO indicates that the state of the denatured Hb is very similar to that of the denatured Mb. In the denatured Hb, quaternary contact of the protein is likely severed, with the denatured protein existing as an independent subunit much like Mb.

• Bulletin of the Korean Chemical Society
• /
• v.20 no.12
• /
• pp.1493-1500
• /
• 1999

The electronic transitions from the ground state to low-lying excited states of CF₃OH have been investigated using high level ab initio quantum mechanical techniques. Also the possible photodissociation procedures of CF₃OH have been considered. The highest level employed in this study is TZP CCSD(T) level of theory. The possible four low-lying excited states can result by the excitation of the lone pair electron (n) in oxygen to σ$^*$ molecular orbital in C-O or O-H bond. The vertical transition (n → σ$^*$) energy is predicted to be 220.5 kcal/mol (130 nm) at TZ2P CISD level to theory. The bond dissociation energies of CF₃OH to CF₃O +H and CF₃+OH have been predicted to be 119.5 kcal/mol and 114.1 kcal/mol, respectively, at TZP CCSD level of theory. In addition, the transition state for the unimolecular decomposition of CF₃OH into CF₂O + HF has been examined. The activation energy and energy separation for this decomposition have been computed to be 43.6kcal/mol and 5.0 kcal/mol including zero-point vibrational energy corrections at TZP CCSD(T) level of theory.ed phenols were also estimated.

• Journal of the Korean earth science society
• /
• v.31 no.4
• /
• pp.348-353
• /
• 2010

The scattered light of solar radiance near the infrared ray area was observed to measure $H_2O$ and $O_2$ absorption lines. The changes of $H_2O$ and $O_2$ equivalent width were calculated on the basis of the measurement. $O_2$ equivalent width showed negative correlation with the amount of solar radiance; $O_2$ equivalent width had a tendency to diminish as the amount of the solar radiance increased and to increase as the amount of the solar radiance decreased. On the other hand, $H_2O$ equivalent width showed the positive correlation with the amount of solar radiance. Especially it was noted that the sum of equivalent width of absorption lines created by $H_2O$ and $O_2$ in a day was fairly constant. It is implied that the constant equivalent width is caused by the complementary development of photodissociation and recombination in $O_2$ and $H_2O$.

• The Bulletin of The Korean Astronomical Society
• /
• v.42 no.2
• /
• pp.43.3-43.3
• /
• 2017

NGC 6822 is a dwarf irregular galaxy in the Local Group and it is located in 500 kpc, further than the Large Magellanic Cloud and the Small Magellanic Cloud. Therefore, we can study star-forming processes by local condition in NGC 6822 instead of tidal force of the Galactic gravitational field. Hubble V is the brightest of several H II complexes in this galaxy. We observed Hubble V by using IGRINS attached on the 2.7 m telescope at the McDonald Observatory in Texas, US in May 2016. We performed a spectral mapping of $15^{{\prime}{\prime}}{\times} 7^{{\prime}{\prime}}$area on H and K bands, and detected emission lines of bright $Br{\gamma}\;{\lambda}2.1661{\mu}m$ and weak He I ${\lambda}2.0587{\mu}m$. Molecular hydrogen lines of 1-0S(1) ${\lambda}2.1218{\mu}m$, 2-1 S(1) ${\lambda}2.2477{\mu}m$, and 1-0 S(0) ${\lambda}2.2227{\mu}m$ was also detected. These emission lines show the structure of an ionized core and excited surface of clouds by far-ultraviolet photons, photodissociation region (PDR). We present three-dimensional maps of emission line distributions through multi slit scanning data and compare these results with the previous study. This presentation shows the physical structure of the star-forming regions and we discuss a PDR model and an evolution of Hubble V complex.

• Bulletin of the Korean Chemical Society
• /
• v.31 no.12
• /
• pp.3771-3776
• /
• 2010

The dynamics of the $CN^-$-ligated ferric cytochrome c (CytcCN) in $D_2O$ at 283 K following Q-band photoexcitation at 575 nm was observed using femtosecond time-resolved vibrational spectroscopy. The equilibrium vibrational spectrum of the CN stretching mode of CytcCN shows two overlapping bands: one main band (82%) at $2122\;cm^{-1}$ with $23\;cm^{-1}$ full width at half maximum (fwhm) and the other band (18%) at $2116\;cm^{-1}$ with $7\;cm^{-1}$ fwhm. The time-resolved spectra show bleaching of the CN fundamental mode of CytcCN and two absorption features at lower energies. The bleach signal and both absorption features are all formed within the time resolution of the experiment (< 200 fs) and decay with a life time of 1.9 ps. One transient absorption feature, appearing immediately red to the bleach signal, results from the thermal excitation of low-frequency modes of the heme that anharmonically couple to the CN fundamental mode, thereby shifting the CN mode to lower energies. The shift of the CN mode decays with a lifetime of 2 ps, equivalent to the time scale for vibrational cooling of the low-frequency heme modes. The other transient absorption feature, which is 3.3 times weaker than the bleach signal and shifted $27\;cm^{-1}$ toward lower energies, is attributed to the CN mode in an electronically excited state where the CN bond is weakened with a lowered extinction coefficient. These observations suggest that photoexcited CytcCN mainly undergoes ultrafast radiationless relaxation, causing photo-deligation of $CN^-$ from CytcCN highly inefficient. As also observed in $CN^-$-ligated myoglobin, inefficient ligand photodissociation might be a general property of $CN^-$-ligated ferric hemes.

• Korean Journal of Optics and Photonics
• /
• v.1 no.1
• /
• pp.46-51
• /
• 1990

Laser plasma was generated by a 1GW iodine photodissociation laser ($\lambda$=1.315$\mu\textrm{m}$, E=12.7J) whose output beam was focused on a molybdenum target surface. The experiment was conducted in a vacuum chamber under 1D-sTorr and several tens of laser shooting were necessary for sufficient exposure for the PBS resist of 111m thickness. Aluminium was coated on the top of the resist by 0.1$\mu\textrm{m}$ thickness which acts as an X-ray filter to cut off the visible and the ultraviolet lights. A bio-specimen was put directly on the aluminium coated resist and located at a distance of 3 cm from the X-ray source. The replicas of a steel mesh, spider's web. and a red blood cell were obtained by this technique and were observed by Nomarski microscope and SEM. The limitation of its resolution is determined by the X-ray source size and Fresnel diffraction effect, and its theoretical prediction is well matched with the experimental results. In this experiment, a resolution better than 0.1$\mu\textrm{m}$ could be obtained. ained.

• The Bulletin of The Korean Astronomical Society
• /
• v.44 no.1
• /
• pp.70.4-70.4
• /
• 2019

Complex organic molecules (COMs) are increasingly observed in the environs of young stellar objects (YSOs), including hot cores/corinos around high-mass/low-mass protostars and protoplanetary disks. It is widely believed that COMs are first formed in the ice mantle of dust grains and subsequently released to the gas by thermal sublimation at high temperatures (T>100 K) in strong stellar radiation fields. In this paper, we report a new mechanism that can desorb COMs from icy grain mantles at low temperatures (T<100K), which is termed rotational desorption. The rotational desorption process of COMs comprises two stages: (1) ice mantles on suprathermally rotating grains spun-up by radiative torques (RATs) are first disrupted into small fragments by centrifugal stress, and (2) COMs and water ice then evaporate rapidly from the tiny fragments (i.e., radius a <1nm) due to thermal spikes or enhanced thermal sublimation due to increased grain temperature for larger fragments (a>1 nm). We discuss the implications of rotational desorption for releasing COMs and water ice in the inner region of protostellar envelopes (hot cores and corinos), photodissociation regions, and protoplanetary disks (PPDs). In shocked regions of stellar outflows, we find that nanoparticles can be spun-up to suprathermal rotation due to supersonic drift of neutral gas, such that centrifugal force can be sufficient to directly eject some molecules from the grain surface, provided that nanoparticles are made of strong material. Finally, we find that large aggregates (a~ 1-100 micron) exposed to strong stellar radiations can be disrupted into individual icy grains via RAdiative Torque Disruption (RATD) mechanism, which is followed by rotational desorption of ice mantles and evaporation of COMs. In the RATD picture, we expect some correlation between the enhancement of COMs and the depletion of large dust grains in not very dense regions of YSOs.