• Journal of Photoscience
• /
• v.9 no.2
• /
• pp.86-89
• /
• 2002

Blue light activates proton pump, and creates electrical gradient across the plasma membrane and drives $K^{+}$ uptake in stomatal guard cells. In this presentation, we provide evidence for regulatory mechanisms of the pump and the identification of blue light receptor. The pump is shown to be the plasma membrane H$^{+}$- ATPase and is activated through phosphorylation of the C-terminus. Phosphorylation occurred and 14-3-3 protein bound to the phosphorylation site. The binding of 14-3-3 protein was required for the H$^{+}$-ATPase activation. We also found that phot1 phot2 double mutant does not respond to blue light but other mutants respond to blue light by stomatal opening. However, all these mutants are capable of stomatal opening in the presence of fusicoccin, an activator of the H$^{+}$-ATPase. These results suggest that both photl and phot2 act as blue light receptors in guard cells.d cells.

• The Bulletin of The Korean Astronomical Society
• /
• v.42 no.2
• /
• pp.70.2-70.2
• /
• 2017

Transit event of exoplanet is a good example of observational studies with youth, because the event is geometrically simple and its analysis is essential to astronomical observation. Therefore, we developed the package of data reduction and aperture photometry in Python for educational purpose. In 27 July, we observed the transit event of TrES-3b with the students of "NYSC Space Science Club" program, and presented the Python package, PyPhotW for data reduction and aperture photometry. PyPhotW consists of simple functions for youth to understand the processes easier. Nonetheless, the photometric results of PyPhotW show a good agreement with those of Source Extractor, ${\Delta}m{\sim}-0.01{\pm}0.03$ and $-0.04{\pm}0.08$ for TrES-3b and TrES-5b time-series observations in 27 - 28 July.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
• /
• v.26 no.2
• /
• pp.112-116
• /
• 2015

Background and Objectives : Aerodynamic analysis is an examination which provides information regarding various vocalization measures indicating laryngeal efficiency. Voice evaluation using such examination must be capable of distinguishing between normal to abnormal voice. It also observes variables on aerodynamic characteristics by gender in regards to patients of vocal disorders, especially of vocal cord paralysis and vocal polyp, and compares the conditions before and after surgery. This paper therefore, seeks to build a framework for establishing standard levels of aerodynamical characteristic on vocal disorders. Subjects and Methods : The study was intended for a total number of 20 patients with vocal polyp or unilateral vocal cord paralysis. Those with the vocal polyp underwent laryngomycroscopy surgery and the vocal cord paralysis, vocal fold injection using Restylane. Aerodynamic analysis fulfilled the Maximum sustained Phonation (MXPH) and Voicing Efficiency (VOEF) by using PAS Model 6600 (KayPENTAX, USA). Results : In MXPH, increase in PHOT were evident with vocal polyp after surgery. As for patients with vocal cord paralysis, MAXDB, MEADB, DHODB, PHOT all have increased and MEAP, PEF, MEAF decreased after surgery. In VOEF, patients with vocal cord paralysis who underwent surgery showed increase in MAXDB, MEADB, DHODB, FET100, ARES, but decreases in PEF, TARF. Conclusion : Overall, it can be concluded that patients with the vocal polyp and vocal cord paralysis seemed to get closer to the normal values after than before surgery in majority of measures. This confirms that the function of their vocal cord has improved nearly to normality through operations.

• Phonetics and Speech Sciences
• /
• v.11 no.3
• /
• pp.69-76
• /
• 2019

This study aimed to investigate the effects of vocal aerobic treatment (VAT) on the improvement of voice in patients with voice disorders. Twenty patients (13 males, 7 females) were diagnosed with voice disorders on the basis of videostroboscopy and voice evaluations. Acoustic evaluation was performed with the Multidimensional voice program (MDVP) and Voice Range Profile (VRP) of Computerized Speech Lab (CSL), and aerodynamic evaluation with PAS (Phonatory Aerodynamic System). The changes in F0, Jitter, Shimmer, and NHR before and after treatment were measured by MDVP. F0 range and Energy range were measured with VRP before and after treatment, and the changes in Expiratory Volume (FVC), Phonation Time (PHOT), Mean Expiratory Airflow (MEAF), Mean Peak Air Pressure (MPAP), and Aerodynamic Efficiency (AEFF) with PAS. Videostroboscopy was performed to evaluate the regularity, symmetry, mucosal wave, and amplitude changes of both vocal cords before and after treatment. Voice therapy was performed once a week for each patient using the VAT program in a holistic voice therapy approach. The average number of treatments per patient was 6.5. In the MDVP, Jitter, Shimmer, and NHR showed statistically significant decreases (p < .001, p < .01, p < .05). VRP results showed that Hz and semitones in the frequency range improved significantly after treatment (p < .01, p < .05), as did PAS, FVC, and PHOT (p < .01, p < .001). The results for videostroboscopy, functional voice disorder, laryngopharyngeal reflux, and benign vocal fold lesions were normal. Thus, the VAT program was found to be effective in improving the acoustic and aerodynamic aspects of the voice of patients with voice disorders. In future studies, the effect of VAT on the same group of voice disorders should be studied. It is also necessary to investigate subjective voice improvement and objective voice improvement. Furthermore, it is necessary to examine the effects of VAT in professional voice users.

• Journal of Astronomy and Space Sciences
• /
• v.11 no.2
• /
• pp.208-231
• /
• 1994

We have undertaken CCD observations of fie이 standard stars with H$\beta$ photometric system to investigate the reliability of H$\beta$ CCD photometry. Flat fielding with dome flat and sky flat for H$\beta$w and H$\beta$n filter was compared with that of B filter in UBV system and, from these, we have not found any difference. It was confirmed that there is a good linear relationship between our H$\beta$ values observed with 2.3m reflector and standard values. However, H$\beta$ values observed with 60cm reflector at Sobaeksan Astronomy Observatory showed very poor relationship. To investigate the accuracy of H$\beta$ CCD photometry for fainter objects, open cluster NGC2437 was observed and reduced with DoPHOT, and the results were compared with those for photoelectric photometry of Stetson(1981).

• Proceedings of the Korean Geotechical Society Conference
• /
• 2008.03a
• /
• pp.427-434
• /
• 2008

There were lots of landslide due to the strong rainfall in In-Je province from 11th ~ 17th July, 2007. In general the landslides have the very close relation with rainfall, the study area also have the strong relation to the rainfall, and there were some place in study area where no failure occurred at same rainfall. So in this study, to analyze the factor of failure, we used GIS program, aero phot of before and after the failure and made thematic map.

• Journal of Photoscience
• /
• v.9 no.2
• /
• pp.335-337
• /
• 2002

Blue light (BL) induces stomatal opening through activation of H$^{+}$ pump, which creates electrical gradient across the plasma membrane for $K^{+}$ uptake into guard cells. The pump is the plasma membrane H$^{+}$ -ATPase and is activated via phosphorylation of the C-terminus with concomitant binding of the 14-3-3 protein. The opening is initiated by the perception of BL through phototropin (phot), which are recently identified as BL receptors in stomatal guard cells. In this study, we provide the biochemical evidence for phots as BL receptors in stomatal guard cells. vfphot was phosphorylated reversibly by BL, and phosphorylation levels of vfphot increased earlier than those of the plasma membrane W-ATPase. BL-dependent phosphorylations of vfphot and H$^{+}$-ATPase showed similar fluence dependency. Staurosporin, an inhibitor of serine/threonine protein kinase, and diphenyleneiodonium chloride (DPI), an inhibitor of flavoprotein, inhibited BL-dependent phosphorylations of vfphot and H$^{+}$ -ATPase. These results indicate that vfphot acts as a BL-receptor mediating stomatal opening.l opening.

• The Bulletin of The Korean Astronomical Society
• /
• v.43 no.1
• /
• pp.40.4-41
• /
• 2018

We introduce a coordinate catalog for photometry of the KMTNet Galactic bulge observation program and how to find the microlensing event candidates in the photometry result. Basically, the KMTNet bulge program is monitoring a total of 27 target fields (108 deg2) with four different cadences of 0.5, 1.0, 2.5, and 5.0 hours. In order to measure the stellar flux of each target, we made a 'observation coordinate catalog' by using the 'OGLE-III catalog' and 'DoPhot package'. The catalog contains approximately 0.3 billion stars in the bulge fields. We also search for a microlensing event candidates by means of the 'Event Finder algorithm' which calculates the restricted single-lens fitting (t0, teff, u0; u0 = 0 or 1) model. As a result, we found more than 2,000 microlensing event candidates per each year including about 700 events from the other survey groups such as OGLE and MOA. In this year, we will improve our current pipeline system, e.g. upgrading the catalog and applying real-time photometry.