• Microbiology and Biotechnology Letters
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• v.27 no.4
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• pp.286-291
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• 1999

By using autoradiography and phosphate medium, high phosphate accumulating bacteria were isolated from the soil of protected cultivation area and activated sludge. Selected strain, PO8, was gram-negative, rod/spherial(0.5~0.6$\times$1.0~1.2${\mu}{\textrm}{m}$ in size) and non-motile. PI4, another selected strain, was gram-negative, rod(0.4~0.5$\times$1.5~1.6${\mu}{\textrm}{m}$ in size) and motile. It also had flagella. According to their morphological, physiological and biochemical properties, the stains were identified as Acinetobacter lwoffi PO8 and Chromobacterium lividum PI4, respectively. A. lowoffi PO8 and C. lividum PI4 cultured in the P-1 medium containing 150ppm phosphate were able to uptake high phosphate up to 92% and 85%, respectively after 24 hours at 3$0^{\circ}C$ during liquid culture.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.113-118
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• 2002

Laboratory experiments were aimed to evaluate the effect of nitrate as a electron acceptor during the biological phosphorus uptake and to investigate the microbial community. Anaerobic-anoxic sequencing batch reactor (SBR) compared the removal behaviour to anaerobic-oxic SBR, both SBRs maintained lower effluent quality with 1.0 mgp/1. Anaerobic-anoxic SBR was able to remove additional 5.0 to 7.0 mg (P+N)/ι than other biological nutrient removal (BM) system. Therefore, it was proposed that the anaerobic-anoxic SBR was more effective at weak sewage. From the results of the maicrobial community analysis, it can be inferred that denitrifying bacteria and polyphosphate accumulating bacteria coexist in anaerobic-anoxic SBR during stable condition for removing the nitrogen and phosphorus. Particularly, it was suggested that the Zoogloea ramigera in the $\beta$-subclass of proteobacteria and the Alcaligenes defragrans of the Rhodocyclus group in the $\beta$-subclass of proteobacteria played a major role for removing the nitrogen and phosphorus as dPAOs (denitrifying phosphate accumulating organisms).

• Journal of Microbiology and Biotechnology
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• v.4 no.2
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• pp.126-133
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• 1994

A Pseudomonas putida strain able to accumulate high amount of polyesters of medium-chain-length 3-hydroxyalkanoic acids ($PHA_{MCL)$) was isolated from soil in a landfill site using an enrichment technique. Culture condition of the isolated strain for polyester production in a one-step culture was optimized in a mineral-salts medium against pH and concentrations of ammonium sulfate, carbon source(e.g., octanoate), and phosphate. The optimal values for maximal cell growth and PHA accumulation were: pH; 7$\sim$8, $(NH_4)_2SO_4$; 8 mM, octanoate; 40 mM. The optimum temperature was in the range of $20\sim30^{\circ}C$, which was rather broader than in other bacteria. Cell growth was strongly inhibited by the phosphate limitation to less than 1 mM. An increase of phosphate concentration above 1 mM showed little effect on cell growth and polyester accumulation. When the strain was grown on octanoate under this optimized condition it produced 3.4 g dry biomass per liter and yielded 1.7 g PHA per liter amounting to 53 wt% of dry cells. The monomer units composing the polyester synthesized from octanoate were 3-hydroxyoctanoate (3HO), 3-hydroxycaproate (3HC), and 3-hydroxybutyrate (3HB) (85:13:2, mole ratio). Other low linear $C_3\simC_{10}$ monocarboxylic acids were also tested for polyester production.

• Korean Journal of Environmental Agriculture
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• v.31 no.2
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• pp.185-191
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• 2012

Background: The removal of phosphate(P) in the wastewater is essential for the prevention of eutrophication in the river and stream. This study was initiated to evaluate the P removal by three strains of bacteria isolated from industrial wastewater. The three strains of bacteria, A1, A2, and A3, isolated were identified as Stenotrophomonas maltophilia strain CUPS 3, Rhodococcus erythropolis strain Sco-C01, Bacillus sp. 3434BRRJ, respectively. METHODS AND RESULTS: The experiments evaluating the effects of temperature, P concentration, aeration, and carbon sources on P removal by Bacillus sp. 3434BRRJ were performed in the following conditions: temperature, 15, 25 and $30^{\circ}C$; P concentrations, 20, 30, and 40 mg/L; oxygen condition, aerobic, anaerobic/aerobic conditions; carbon sources, glucose, acetate and mixture of glucose and acetate. As a result, the best optimum conditions for P removal by Bacillus sp. 3434BRRJ were as follows: temperature, $30^{\circ}C$; P concentration, 20 mg/L; carbon sources, mixture of glucose and acetate; oxygen concentration, anaerobic and aerobic conditions. The P removal efficiencies by Bacillus sp. 3434BRRJ, Stenotrophomonas maltophilia strain CUPS, and Rhodococcus erythropolis strain Sco-C01 were 99%, 50%, 20%, respectively. CONCLUSION: As a result, the best optimum conditions for P removal by Bacillus sp. 3434BRRJ selected and used in this study were as follows: temperature, $30^{\circ}C$; P concentration, 20 mg/L; carbon sources, mixture of glucose and acetate; oxygen concentration, anaerobic and aerobic conditions.