• Journal of Advanced Navigation Technology
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• v.18 no.1
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• pp.44-49
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• 2014

In recent, watermarking technology have been paying attention to methods avoiding illegal use and reproduce of digital contents. Then, in order to protect the right of digital contents, a watermark image is inserted into original images. In different watermarking methods, several technologies using Ant Colony Algorithm have been studied. In this paper, we propose a watermarking method using a discrete optimization method in the ants colony algorithm. This proposed method resembls the process that ants follow the pheromone traps to find out food. And when a watermark image is inserted into original images, the proposed method considers the deployment of obstacles or the balance between cells in the entire digital image. Simulation results show that the proposed method is increased in robustness of watermarked image and is decreased in the perceptibility of watermarking compared to the previous methods.

• The Plant Pathology Journal
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• v.19 no.1
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• pp.30-33
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• 2003

The chestnut blight fungus, Cryphonectria parasitica, and its hypovirus aye a useful model system in the study of the mechanisms of hypoviral infection and its consequences, such as a biological control of fungal pathogens. Strains containing the double-stranded (ds) RNA viruses Cryphonectria hypovirus 1 show characteristic symptoms of hypovirulence and display hypovirulence-associated changes, such as reduced pigmentation, sporulation, laccase production, and oxalate accumulation. Interestingly, symptoms caused by hypoviral infection appear to be the result of aberrant expression of a number of specific genes in the hypovirulent strain. Several viral regulated fungal genes are identified as cutinase gene, Lac1, which encodes an extracellular laccase, Crp, which encodes an abundant tissue-specific cell-surface hydrophobin that mediates physical strength, and Mf2/1 and Mf2/2, which encode pheromone genes involved in poor sporulation in the presence of hypo-virus. Since the phenotypic changes in the fungal host are pleiotropic, although coordinated and specific, it has been suggested that the hypovirus disturbs one or several regulatory pathways (Nuss,1996). Accordingly, several studies have shown the implementation of a signal transduction pathway during viral symptom development. Although further studies are required, hypovirulence and its associated symptom development due to the hypoviral regulation of a fungal hetero-trimeric G-protein have been suggested. In addition, recent studies have shown the presence of a novel protein kinase gene cppk1 and its transcriptional upregulation by hypovirus. In this review, the presence of important components in signal transduction pathway, their putative biological function, and viral-specific regulation will be addressed.

• Journal of Microbiology
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• v.34 no.1
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• pp.30-36
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• 1996

We have exmained the re-establishment of HIMRE mediated silencing function on the transcriptional activity of yeast heast shock gene HSP82. To test whether the onset of SIR repression can occur in growing cells in the rpesence of a potent inhibitor of DNA replication, HMRa/HSP82 strains with SIR4- and SIR4S$^{+}$ genetic backgrounds were arrested in S phase by incubation of a culture in 200 mM hydroxyurea for 120 min. It was clear that following a 20 minute heat shock, silencing of the HMRa/HSP82 allele in cells pretreated with hydroxyurea does occur in a SIR4-dependen fashion, even though the kinetics of repression appears to be substantially delayed. We also have tested whether re- establishement of silencing at the HMR/hsp82 locus can occur in G1-arrested cells. Cell cycle arrest at G1 phase was achieved by treatment of early log a cell cultures with .alpha.-factor mating pheromone, which induces G1 arrest. The result suggests that passage through S phase (and therefore DNA replication) is nor required for re-establishing silencer-mediated repression at the HMNRa/HSP82 locus. Finally, to test whether de nono protein synthesis is required for re-establishment of silencer-mediated repression, cells were pretreated with cycloheximide (500 /.mu.g/ml) 120 min. It was apparent that inhibiting protein synthesis delays, but does not prevent, re-establishment of silencer-mediated repression. Altogether, these results indicate that re-establishment of silencer-mediated repression is not dependent on the DNA replication and has no requirement for protein synthesis.s.

• Mycobiology
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• v.37 no.3
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• pp.171-182
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• 2009

Many aspergilli that belongs to ascomycetes have sexuality. In a homothallic or self-fertile fungus, a number of fruiting bodies or cleistothecia are formed in a thallus grown from a single haploid conidia or ascospores. Genome-sequencing project revealed that two mating genes (MAT) encoding the regulatory proteins that are necessary for controlling partner recognition in heterothallic fungi were conserved in most aspergilli. The MAT gene products in some self-fertile species were not required for recognition of mating partner at pheromone-signaling stage but required at later stages of sexual development. Various environmental factors such as nutritional status, culture conditions and several stresses, influence the decision or progression of sexual reproduction. A large number of genes are expected to be involved in sexual development of Emericella nidulans (anamorph: Aspergillus nidulans), a genetic and biological model organism in aspergilli. The sexual development process can be grouped into several development stages, including the decision of sexual reproductive cycle, mating process, growth of fruiting body, karyogamy followed by meiosis, and sporulation process. Complicated regulatory networks, such as signal transduction pathways and gene expression controls, may work in each stage and stage-to-stage linkages. In this review, the components joining in the regulatory pathways of sexual development, although they constitute only a small part of the whole regulatory networks, are briefly mentioned. Some of them control sexual development positively and some do negatively. Regarding the difficulties for studying sexual differentiation compare to asexual one, recent progresses in molecular genetics of E. nidulans enlarge the boundaries of understanding sexual development in the non-fertile species as well as in fertile fungi.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.167-173
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• 1993

Pirimiphos - methyl was fogged in a tobacco warehouse to determine the proper dose rate, Small wire cages, containing adults and larvae of cigarette beetle (Lasiodema senicorne F.) were put at different altitude in the warehouse of which the holding capacity was about 250m3. At recommended dose rate, 0.4ml/m3(25% ai), it brought about 100% mortality of adults and 98.8% -100% mortality of larvae. When the dose rate was reduced to about half the recommended one, 97.6 and 96.4% mortality could still be obtained against adults and larvae, respectively. The number of the beetles monitored by sex - pheromone trap was greatly reduced after the fogging in the warehouses at 4 geographic places in Korea. When the warehouses were ventilated for one hour immediately after the fogging done for 48 hours, workers could not detected any opposing smell in the warehouses. And panel members responded positively that there was no negative effect on the taste of the cigarettes made of the fogged tobacco. Although the residue of the chemical was detected from the cigarettes in 4 of 5 cases, the residue was 1.1-1.3$\mu\textrm{g}$/20 cigarettes except for one case from which 3.3$\mu\textrm{g}$/20 cigarettes residue was detected. The relatively low residue is about l/450-1/150 of the WHO/FAO tolerant level.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.3
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• pp.269-274
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• 2015

Chronic inflammation has been proposed as one of the main molecular mechanisms of aging and age-related diseases. Although evidence in humans is limited, short-term calorie restriction (CR) has been shown to have anti-inflammatory effects in aged experimental animals. We reported on the long-term treatment of daumone, a synthetic pheromone secreted by Caenorhabditis elegans in an energy deficient environment, extends the life-span and attenuates liver injury in aged mice. The present study examined whether late onset short-term treatment of daumone exerts anti-inflammatory effects in the livers of aged mice. Daumone was administered orally at doses of 2 or 20 mg/kg/day for 5 weeks to 24-month-old male C57BL/6J mice. Increased liver macrophage infiltration and gene expression of proinflammatory cytokines in aged mice were significantly attenuated by daumone treatment, suggesting that short-term oral administration of daumone may have hepatoprotective effects. Daumone also dose-dependently suppressed tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$ )-induced nuclear factor-${\kappa}B$ (NF-${\kappa}B$) phosphorylation in HepG2 cells. The present data demonstrated that short-term treatment of daumone has anti-inflammatory effects in aged mouse livers possibly through suppression of NF-${\kappa}B$ signaling and suggest that daumone may become a lead compound targeting aging and age-associated diseases.

• Korean journal of applied entomology
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• v.30 no.4
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• pp.233-240
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• 1991

A female Brachymeria lasus (Walker) was exposed to one or four pupae of host Hyphantria cunea Drury for 20 minutes within a petri dish $(1.5\times9cm)$ to learn factors influencing the host discrimination by the parasitoid. It rejected the parasitized host with its rejection ratio 73.3% for the one pupal exposure vs 100% for the four pupal exposure scheme. The parasitoid could discriminate the parasitized host by its antennal drumming and ovipositor probing due to a pheromone-like external marking which is effective only for a certain peiod of time, and a host quality associated with the parasitoid development, respectively.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.340-344
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• 2002

A gene, npl, encoding neopullulanase from Paenibacillus sp. KCTC 8848P was cloned and expressed in Escherichia coli. It consisted of an open reading frame of 1,530 bp for a protein that consisted of 510 amino acids with a molecular weight of 58,075 Da. The deduced amino acid sequence of the neopullulanase gene had $92\%$ identity with the neopullulanase of Bacillus polymyxa. The npl gene was also expressed in Saccharomyces cerevisiae secreting Schwanniomyces occidentalis glucoamylase (GAM1) under the control of the yeast actin gene (ACT1) promoter. Secretion of the neopullulanase was directed by the yeast mating pheromone ${\alpha}$ -factor ($MF{\alpha}1$) prepro region. Enzyme assays confirmed that co-expression of npl and GAM1 enhanced starch and pullulan degradation by S. cerevisiae.

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2006.11a
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• pp.138-138
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• 2006

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.6-8
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• 2011

In order to produce the organic foods in accord with international standard, organic seeds should be used in organic farming. This study was conducted to establish the stable production of organic seed of waxy com by examining the growing characteristics, seed yield, and the economy for seed production by organic farming. The optimal sowing timing for organic seed production of waxy com hybrid was within 10 days of the $1^{st}$ of May with yield of 88~90% of conventional seed production. The optimal planting density was 41,600 plants/ha ($80{\times}30$ cm) for organic seed production of waxy com. The weight of 100 seeds and seed productivity increased at the planting ratio of 2:1 mother plant:male plant. Growth and seed production were improved by removing male plant at 7~10 days after silking. Organic fertilizer (mixed oil cake) was applied at a rate of 4~6 Mg/ha before sowing. Black plastic mulching was used for weed control. In addition, sex pheromone trap and bio-control agents were used for safe pest control and low labour cost.