• Title/Summary/Keyword: phenotypic selection

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Discovery of Performance Traits-Linked Microsatellite Markers in Channel Catfish (Ictalurus punctatus)

  • Kim, Soon-Hag
    • Journal of Aquaculture
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    • v.18 no.2
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    • pp.130-132
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    • 2005
  • Genomics research has two ultimate applied goals: to Isolate and clone genes of economic importance for bio-technology and gene-assisted selection (GAS), and to locate and use markers for marker-assisted selection (MAS) in selective breeding programs. To this end, we have identified linked markers for feed conversion efficiency growth rate, and disease resistance to enteric septicemia of catfish (ESC). Three microsatellite markers Ip266, Ip384, and Ip607 were identified to be linked to feed conversion efficiency. Similarly one marker each was identified to be linked to growth rate (Ip607) and disease resistance to ESC (Ip477). Ip607 marker linked to both growth rate and feed conversion efficiency, indicating that the QTL for both growth rate and feed conversion efficiency may either be the same or located in the same chromosomal region in the catfish genome. On phenotypic evaluation, certain traits such as growth rate can be accurately evaluated by body weight evaluation while other traits such as disease resistance can be quite complex. The linked DNA markers will be highly useful for MAS programs and for directing further efforts of genomic mapping for important quantitative traits.

Phenotypic Correlation for Concentrations of Hormones and Metabolic Materials and Growth and Carcass Traits in Hawoo (한우에서 혈중 호르몬 및 대사물질 농도와 성장 및 도체 형질에 대한 표현형 상관에 관한 연구)

  • 전기준;최재관;이명식;정영훈;정호영;이종경;임석기;이창우;박정준
    • Journal of Embryo Transfer
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    • v.18 no.3
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    • pp.203-213
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    • 2003
  • Examination of correlation between blood compositions and economic traits is very important to improve selection accuracy and predict performance ability of Hanwoo, which may be impacted into the determination of feeding management as well as marketing places. This study was aimed to provide possible procedures of Hanwoo improvement as of early individual selection based on the phenotypic correlation between blood compositions and economic traits using 866 of Hanwoo managed at National Livestock Research Institute. Phenotypic correlation between blood compositions and economic traits was estimated for steer and bulls. BUN in steer and albumin and IGF-1 in bull were highly correlated with 24 mo of weight. Average daily gain of 21 to 24 mo was highly correlated with calcium in steer and albumin in bull. Back-fat thickness and marbling score were highly correlated with BUN in steer and bull, and meat yield index was highly correlated with calcium in steer and testosterone in bull. However, BUN, which is highly correlated with meat quality, was negatively correlated with meat yield index in steer and bull.

Validation of selection accuracy for the total number of piglets born in Landrace pigs using genomic selection

  • Oh, Jae-Don;Na, Chong-Sam;Park, Kyung-Do
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.2
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    • pp.149-153
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    • 2017
  • Objective: This study was to determine the relationship between estimated breeding value and phenotype information after farrowing when juvenile selection was made in candidate pigs without phenotype information. Methods: After collecting phenotypic and genomic information for the total number of piglets born by Landrace pigs, selection accuracy between genomic breeding value estimates using genomic information and breeding value estimates of best linear unbiased prediction (BLUP) using conventional pedigree information were compared. Results: Genetic standard deviation (${\sigma}_a$) for the total number of piglets born was 0.91. Since the total number of piglets born for candidate pigs was unknown, the accuracy of the breeding value estimated from pedigree information was 0.080. When genomic information was used, the accuracy of the breeding value was 0.216. Assuming that the replacement rate of sows per year is 100% and generation interval is 1 year, genetic gain per year is 0.346 head when genomic information is used. It is 0.128 when BLUP is used. Conclusion: Genetic gain estimated from single step best linear unbiased prediction (ssBLUP) method is by 2.7 times higher than that the one estimated from BLUP method, i.e., 270% more improvement in efficiency.

Simple Statistical Tools to Detect Signals of Recent Polygenic Selection

  • Piffer, Davide
    • Interdisciplinary Bio Central
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    • v.6 no.1
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    • pp.1.1-1.6
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    • 2014
  • A growing body of evidence shows that most psychological traits are polygenic, that is they involve the action of many genes with small effects. However, the study of selection has disproportionately been on one or a few genes and their associated sweep signals (rapid and large changes in frequency). If our goal is to study the evolution of psychological variables, such as intelligence, we need a model that explains the evolution of phenotypes governed by many common genetic variants. This study illustrates simple statistical tools to detect signals of recent polygenic selection: a) ANOVA can be used to reveal significant deviation from random distribution of allele frequencies across racial groups. b) Principal component analysis can be used as a tool for finding a factor that represents the strength of recent selection on a phenotype and the underlying genetic variation. c) Method of correlated vectors: the correlation between genetic frequencies and the average phenotypes of different populations is computed; then, the resulting correlation coefficients are correlated with the corresponding alleles' genome-wide significance. This provides a measure of how selection acted on genes with higher signal to noise ratio. Another related test is that alleles with large frequency differences between populations should have a higher genome-wide significance value than alleles with small frequency differences. This paper fruitfully employs these tools and shows that common genetic variants exhibit subtle frequency shifts and that these shifts predict phenotypic differences across populations.

Phenotypic and Genotypic Correlations between Some Characters of Mulberry Trees (상수 각형질 상호간의 표현형상관과 유전상관)

  • 장권열;한경수;민병열
    • Journal of Sericultural and Entomological Science
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    • no.12
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    • pp.1-4
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    • 1970
  • The experimental studies were intended to clarify the effects of leaf yield calculations, and also aimed at estimating the correlations between some characters for the selection of desirable stocks of mulberry trees. The analysis of covariances was the variance components procedures in a replicated trial for the varieties, and also it was used to obtain the genotypic, phenotypic and environmental correlations between the eight characters-branch length, node number, branch diameter, branch number per stock, total branch weight, old branch weight, new shoot and leaf weight, and leaf weight, etc. The results obtained are summarized as follows: Phenotypic, genotypic and environmental covariances are shown in Table 1 and phenotypic correlations, genotypic correlations and environmental correlations are shown in Table 2. Genotypic correlations were slightly higher than the corresponding phenotypic correlations between the characters in the varieties, but the variation in values due to the change of environment appeared between the characters. Genotypic correlations between the leaf yield of mulberry trees and other characters indicated that high leaf yield was genetically correlated with six characters namely branch length, node number, branch number per stock, total branch weight, old branch weight and new shoot weight, etc. It was also observed that lower correlations were appeared between the leaf yield and branch diameter, and branch diameter and other all characters studied in both genotypic and phenotypic correlation coefficients.

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FACTORS AFFECTING AGE STRUCTURES AND GENETIC RESPONSES TO TRUNCATION SELECTION SCHEMES IN A POPULATION WITH OVERLAPPING GENERATIONS

  • Ghaffar, A.;Shimizu, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.4
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    • pp.497-507
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    • 1993
  • Four truncation selection schemes (SSs) were framed to predict and compare the age structures and genetic responses under the influence of various factor employing the scheme-specific algorithms. Two paths of selection, sires (bulls' sires) and dams (bulls' dams) to breed young bulls were considered. Among variable factors, four levels (0.3, 0.5, 0.7, 0.9) of precision of evaluation, five levels (0.0, 0.05, 0.10, 0.15, 0.20 genetic standard deviation) of genetic differences among age classes and 4 levels of proportions selected (for bulls' sire, 0.05, 0.10, 0.125, 0.25, and for bulls' dams 0.02, 0.04, 0.05, and 0.10) contemplated on both paths of selection. The number of age classes for bulls' dams and bulls' sires were 4 or 8 and 2 or 4, respectively. The stayability across age classes for bulls' dams was assumed to be 0.80 or 0.60. The candidates for selection for bulls' sires were equally distributed (0.5 or 0.25) across the age classes. The SS1 (selection on same proportions as candidates' distribution) revealed longest generation lengths and lowest yearly genetic responses. The average ages were youngest and yearly genetic responses were highest in SS4 (selection at each age-specific truncation point with the same average genetic superiority of selected parents across the ages) and followed by SS3 (selection at each agespecific truncation point with same predicted genetic values) and SS2 (selection at common truncation point on phenotypic values) in a population with overlapping generations. The results revealed the importance of choosing suitable selection scheme to acquire maximum yearly genetic responses especially when the genetic differences among age classes are large and the precision of evaluation is relatively low.

Inter Simple Sequence Repeat (ISSR) Polymorphism and Its Application in Mulberry Genome Analysis

  • Vijayan Kunjupillai
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.2
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    • pp.79-86
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    • 2005
  • Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification, genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry, for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.

Novel Reproductive Techniques in Swine Production - A Review

  • Okere, C.;Nelson, L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.3
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    • pp.445-452
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    • 2002
  • The main objective of modern reproductive technologies in pig reproduction is to increase reproductive efficiency and rates of genetic improvement. They also offer potential for greatly extending the multiplication and transport of genetic materials and the conservation of unique genetic resources in reasonably available forms for possible future use. The development and refinement of these technologies is concentrating on gamete and embryo collection, sorting and preservation, in vitro production of embryos, culturing, manipulation of embryos (splitting, nuclear transfer, production of chimeras, establishment embryo stem cells, and gene transfer) and embryo transfer. Also, the development of these novel technologies is facilitated by modern equipment for ultrasonography, microscopy, cryopreservation, endoscopy, and flow cytometry, microinjectiors, micromanipulators and centrifugation. The real impact on herd productivity will come from combining new reproductive techniques with powerful DNA technologies. The new reproductive techniques will allow a rapid turnover of generations, whereas the DNA technology can provide selection, which does not need phenotypic information when the selection decisions are made.

In vitro Selection of Acifluorfen-tolerant Solanum ptycanthum and Phenotypic Variation in Regenerated Plants

  • Yu, Chang-Yeon;Lim, Jeong-Dae;Kim, Myong-Jo;Kang, Won-Hee;Hyun, Tae-Kyoung
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.4
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    • pp.263-268
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    • 2002
  • Acifluorfen-tolerant callus lines of Solanum ptycanthum were isolated by stepwise selection. Growth of the unselected line was completely inhibited at 0.5 uM. while some selected lines grew at 8 uM acifluorfen. Twenty-two of twenty-five acifluorfen-tolerant callus lines regenerated shoots. Many of the regenerated somaclones were variants, differing in leaf shape, leaf color, number of flower parts, flower color, and fertility. The acifluorfen tolerant S. ptycanthum callus lines differed.

Phenotypic and genotypic screening of rice accessions for salt tolerance

  • Reddy, Inja Naga Bheema Lingeswar;Kim, Sung-Mi;Yoon, In Sun;Kim, Beom-Gi;Kwon, Taek-Ryoun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.188-188
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    • 2017
  • Rice (Oryza sativa L.) is one of the major crops that is seriously impacted by global soil salinization. Rice is among those crops where most of the high-yielding cultivars are highly sensitive to salinity. The key to a plant survival under NaCl salt stress is by maintaining a high $K^+/Na^+$ ratio in its cells. Selection for salinity tolerance genotypes of rice based on phenotypic performance alone is less reliable and will delay in progress in breeding. Recent advent of molecular markers, microsatellites or simple sequence repeats (SSRs) were used to find out salt tolerant rice genotypes. In the current experiment phenotyping and genotyping studies were correlated to differentiate different rice accessions for salinity tolerance. Eight rice accessions along with check plant Dongjin were screened by physiological studies using Yoshida solution with 50mM NaCl stress condition. The physiology studies identified four tolerant and four susceptible accessions based on their potassium concentration, sodium concentration, $K^+/Na^+$ ratio and biomass. 17 SSR markers were used to evaluate these rice accessions for salt tolerance out of which five molecular markers were able to discriminate tolerant accessions from the susceptible accessions. Banding pattern of the accessions was scored comparing to the banding pattern of Dongjin. The study identifies accessions based on their association of $K^+/Na^+$ ratio with molecular markers which is very reliable. These markers identified can play a significant role in screening large set of rice accessions for salt tolerance; these markers can be utilized to improve salt tolerance of commercial rice varieties with marker-assisted selection (MAS) approach.

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