• Title/Summary/Keyword: phase-contrast

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Conformational Studies of Gaseous Proteins Using Mass Spectrometry

  • Oh, Han-Bin
    • Proceedings of the Korean Biophysical Society Conference
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    • 2003.06a
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    • pp.27-27
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    • 2003
  • Conformations of the +5 to +13 charge state of ubiquitin ions have been studied in the gas phase by an Electron Capture Dissociation (ECD) mass spectrometry (MS) technique. This approach has showed that the conformations of the gaseous ions change from the compact to extended structures as the number of protons on the protein ions increases, consistent with previous collisional cross-section measurements by an ion-mobility MS. However, this observation is in contrast to that of the solution-phase where the unique native structure is usually found. The (un)folding stability and kinetics of these gaseous ions were further investigated experimentally using gradual blackbody-radiation or sudden laser-induced thermal heating, respectively. These studies have provided the evidence that the thermodynamics and kinetics of protein (un)folding in the gas phase are quite different from those of the native aqueous proteins.

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Modulated Carrier Control for Single-Phase Active Power Filter

  • Son, Gibong;Kim, Hyejin;Cho, Bo-Hyung
    • Proceedings of the KIPE Conference
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    • 2013.07a
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    • pp.218-219
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    • 2013
  • In this paper, an improved modulated carrier control for a single-phase shunt active power filter(SAPF), which eliminates harmonic currents at the ac main drawn by nonlinear loads, is introduced. In contrast to conventional methods, since this control method does not need to generate a current reference for SAPF, only the ac main current is sensed instead of the load current and SAPF current. With the proposed control method, the ac main current is directly controlled to be sinusoidal and in phase with the supplied ac voltage by simply comparing the modulated carrier signal with the ac main current. The operation of the proposed SAPF is analyzed and simulation results verify its performance.

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Set-Based Multi-objective Design Optimization at the Early Phase of Design(The First Report) : Theory and Design Support System (초기 설계단계에서의 셋 베이스 다목적 설계 최적화(제1보) : 이론 및 설계지원 시스템)

  • Nahm, Yoon-Eui
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.34 no.2
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    • pp.112-120
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    • 2011
  • The early phase of design intrinsically contains multiple sources of uncertainty in describing design, and nevertheless the decision-making process at this phase exerts a critical effect upon drawing a successful design. This paper proposes a set-based design approach for multi-objective design problem under uncertainty. The proposed design approach consists of four design processes including set representation, set propagation, set modification, and set narrowing. This approach enables the flexible and robust design while incorporating designer's preference structure. In contrast to existing optimization techniques, this approach generates a ranged set of design solutions that satisfy changing sets of performance requirements.

A method for uniform current distribution of HTS cable using Inter-Phase Transformers (Inter-Phase Transformers를 이용한 고온초전도 케이블의 층간 전류 등분배 방안)

  • Choi, Yong-Sun;Yim, Seong-Woo;Sim, Jung-Wook;Hwang, Si-Dole;Park, In-Gyu
    • Proceedings of the KIEE Conference
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    • 2003.07b
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    • pp.973-975
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    • 2003
  • Uniform current distribution among conductor layers in HTS cables using IPTS (inter-phase transformers) was proposed. Conventional methods for current distribution, in which resistors are inserted to conductor layers, causes additional loss. In contrast, IPTS, which use magnetic coupling, make it possible that the current in parallel circuits is distributed uniformly with any load, and minimize the loss. In this study, IPTS were designed and fabricated for examination of uniform current distribution in the conductor layers of HTS cables. The ITP was designed through calculation of its impedance that can cancel the inductance of the conduction layers.

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Effect of SDS on Retention of Nucleic Acid Components in High-Performance Liquid Chromatography

  • Kim Yong-Nam;Choi Kyeung-Soo;Lee Dai-Woon;Phyllis R. Brown
    • Bulletin of the Korean Chemical Society
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    • v.13 no.3
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    • pp.240-245
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    • 1992
  • The effect of the addition of sodium dodecyl sulfate (SDS) to a buffered mobile phase (pH 3.4) on the retention of nucleotides, nucleosides and bases was investigated with a polyvinyl alcohol (PVA) column. Depending on the concentration of SDS, two different trends in the retention of nucleosides and bases containing an $NH_2$ group were observed. If the concentration of SDS was less than 5.5 mM, the retention of compounds containing an $NH_2$ group increased as the concentration of SDS in the mobile phase increased. In contrast, if the concentration was greater than 5.5 mM, the retention of compounds containing an $NH_2$ group decreased. Thus, the SDS acted as an ion-pairing reagent at lower concentration but formed micelles at higher concentrations. The retention behavior of the nucleosides and bases in the presence of a micellar concentration of SDS in the mobile phase on the PVA column was compared to the retention behavior on other types of columns.

Single cell gap polymer-stabilized blue-phase transflective LCDs using internal nanowire grid polarizer

  • Cui, Hong-Qing;Ye, Zhi-Cheng;Hu, Wei;Lin, Xiao Wen;Chung, T.C.;Jen, Tean-Sen;Lu, Yan-Qing
    • Journal of Information Display
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    • v.12 no.3
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    • pp.115-119
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    • 2011
  • Optically isotropic liquid crystal (LC) mixture such as blue-phase LC and nanostructured LC composites exhibit the advantages of fast response time, high contrast ratio and wide-viewing angle due to the induced birefringence along the horizontal electric field. Utilizing this mixture, a novel single cell gap in-plane switching-type polymer-stabilized blue-phase transflective liquid crystal display by embedding the nanowire grid polarizer as a polarization-dependent reflective polarizer in the R region is proposed. This device can be used as a normal black mode without any quarter-wave plate or patterned in-cell phase retarder. Moreover, the transmittance is identical to the reflectance so that it will be suitable for single gamma driving. Detailed electro-optic performances, such as voltage-dependent light efficiency and viewing angle of the proposed device configuration, are investigated.

Phase Transition Characteristics in $Ge_xSb_{100-x}$ Film for Optical Storage Media

  • Park Tae-jin;Kang Myung-jin;Choi Se-young
    • 정보저장시스템학회:학술대회논문집
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    • 2005.10a
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    • pp.124-127
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    • 2005
  • Rewritable optical memory devices such as an CD-RW and DVD+RW are data storage media, which take advantage of the different optical properties in the amorphous and crystalline states of phase change materials. The switching property, structural transformation, transformation kinetics and chemical bindings of $Ge_xSb_{100-x}$($6{\le}x{\le}$34) were studied to investigate the feasibility of applying $Ge_xSb_{100-x}$ alloys in optical memory. The $Ge_xSb_{100-x}$ thin film was deposited by RF magnetron co-sputtering system and phase change characteristics were investigated by X-ray diffraction (XRD), static tester, inductively coupled plasma atomic emission spectrometer (ICP-AES) and atomic force microscopy (AEM). Optimum fiim composition of $Ge_xSb_{100-x}$ was studied and its minimum time fur laser induced crystallization and optical contrast fur phase transition was performed. These results might be correlated with the binding energies between Ge and Sb, and indicate that $Ge_xSb_{100-x}$ have an potential far optical memory applications.

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Accurate Voltage Parameter Estimation for Grid Synchronization in Single-Phase Power Systems

  • Dai, Zhiyong;Lin, Hui;Tian, Yanjun;Yao, Wenli;Yin, Hang
    • Journal of Power Electronics
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    • v.16 no.3
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    • pp.1067-1075
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    • 2016
  • This paper presents an adaptive observer-based approach to estimate voltage parameters, including frequency, amplitude, and phase angle, for single-phase power systems. In contrast to most existing estimation methods of grid voltage parameters, in this study, grid voltage is treated as a dynamic system related to an unknown grid frequency. Based on adaptive observer theory, a full-order adaptive observer is proposed to estimate voltage parameters. A Lyapunov function-based argument is employed to ensure that the proposed estimation method of voltage parameters has zero steady-state error, even when frequency varies or phase angle jumps significantly. Meanwhile, a reduced-order adaptive observer is designed as the simplified version of the proposed full-order observer. Compared with the frequency-adaptive virtual flux estimation, the proposed adaptive observers exhibit better dynamic response to track the actual grid voltage frequency, amplitude, and phase angle. Simulations and experiments have been conducted to validate the effectiveness of the proposed observers.

A Study on the Mechanical Properties of Duplex Stainless Steel Weldment According to Mo Contents

  • Bae, Seong Han;Lim, Hee Dae;Jung, Won Jung;Gil, Woong;Jeon, Eon Chan;Lee, Sung Geun;Lee, Hyo Jong;Kim, In Soo;Lee, Hae Woo
    • Korean Journal of Metals and Materials
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    • v.50 no.9
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    • pp.645-651
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    • 2012
  • This study investigated changes in phase fraction caused by the addition of Mo, as well as the subsequent behaviour of N and its effect on the mechanical properties of welded 24Cr-N duplex stainless steel weld metals. Filler metal was produced by fixing the contents of Cr, Ni, N, and Mn while adjusting the Mo content to 1.4, 2.5, 3.5 wt%. The delta ferrite fraction increased as the Mo content increased. In contrast, the ${\gamma}$ fraction decreased and changed from a round to an acicular shape. Secondary austenite (${\gamma}^{\prime}$) was observed in all specimens in a refined form, but it decreased as the Mo content increased to the extent that it was nearly impossible to find any secondary austenite at 3.5 wt% Mo. Both tensile and yield strengths increased with the addition of Mo. In contrast, the highest value of ductility was observed at 1.41 wt% Mo. At all temperatures, impact energy absorption showed the lowest value at 3.5 wt% Mo, at which the amount of ${\delta}$-ferrite was greatest. There was no significant temperature dependence of the impact energy absorption values for any of the specimens. As the fraction of ${\gamma}$ phase decreased, the amount of N stacked in the ${\gamma}$ phase increased. Consequently, the stacking fault energy decreased, while the hardness of ${\gamma}$ increased.

Susceptibility Contrast Enhancement Imaging in MRI (핵자기 공명 단층 촬영에서의 자화율 강조 영상법)

  • Ro, Y.M.;Mun, C.W.;Lim, T.H.;Cho, Z.H.
    • Proceedings of the KOSOMBE Conference
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    • v.1992 no.05
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    • pp.85-91
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    • 1992
  • In MRI, an image contrast can be developed as a result of the susceptibility effect if an object has paramagnetic substances. This is mainly due to the non-uniform phase distribution or linear gradient developed by the magnetic susceptibility within a voxel, which in turn reduces the signal intensity; e.g., spin phases are dephased and thereby cancel each other resulting in a reduced signal. In this paper, a new concept for manipulating the susceptibility effect through the use of tailored RF pulses is proposed. As potential applications of the method, two different types of tailored RF pulses are introduced: one for susceptibility artifact correction and the other for contrast enhancement. The latter, for example, can be applied to angiography utilizing the paramagnetic property of deoxygenated blood. Both a theoretical study of the method and experimental results are reported.

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