• 제목/요약/키워드: phase-contrast

검색결과 833건 처리시간 0.027초

효모세포의 당과 아미노산의 운반에 관한 연구 II (Sugar and Amino Acid Transport in Yeast)

  • 민경희;권영명
    • 미생물학회지
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    • 제16권4호
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    • pp.148-154
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    • 1978
  • Saccharomyces cerevisiae J170, a mutant, was used for $DL-^{14}C-leucine$ uptake during the sporulation and vegetative stage. $^{14}C-Leucine$ uptake into yeast cells appeared the highest at pH 6.0, indicating the same result of glucose transport, $^{14}C-Leucine$ uptake in sporulation period was higher than in growth phase, showing the evidence that leucine is more required for protein synthesis. This tendency has the evidence tht leucine is more required for protein synthesis. This tendency has the evidence that leucine is more required for protein synthesis. This tendency has been also supported from the result of Km values of leucine uptake in two stages of yeast. Leucine uptake was inhibited by 2,4-dinitrophenol in two stages of yeast. This means that leucine transport system is associated with energy dependent in both stages. The contents of all amino acid in growth phase cells were higher than those of sporulation stge cells, and those of methionine and tyrosine were showed in trace during the sporulation stage. In contrast, the content of glutamic acid in sporulation stage was compared with those of other amino acids.

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Thin-Shell Approach for Elastic Wave Propagation in a Pipe with Liquid

  • Kim Jin Oh;Rose Joseph L.
    • Journal of Mechanical Science and Technology
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    • 제19권5호
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    • pp.1087-1094
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    • 2005
  • This paper presents the validity and limitation of the thin-shell approach for the analysis of elastic wave propagation in a pipe with nonviscous liquid. The phase velocities calculated by the thin-shell approach were compared with those calculated by the thick-cylinder approach. In contrast to the case of the empty pipe, where only two modes were obtained and the first mode was calculated in a limited frequency range, the results for the liquid-filled pipe exhibits a large number of modes due to the large number of branches of the apparent liquid mass. Several of the lowest modes of the waves in a liquid-filled pipe were calculated for various pipe thicknesses in a low frequency range. The thin-shell approach was valid for a reasonable range of pipe thicknesses.

H-Y항체의 처리가 생쥐수정란의 발달에 미치는 영향 (Effect of H-Y Antibody on in vitro Development of Mouse Embryos)

  • 고정재;심호섭;김종배;박홍양;정길생;이경광
    • 한국가축번식학회지
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    • 제10권1호
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    • pp.42-48
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    • 1986
  • These experiments were carried out to develop new techniques identifying XX-bearing embryos prior to implantation by immunological method. Antiserum to histocompatibility-Y(H-Y) antigen was prepared in adult SD(sprague-dawley) female rat by repeated immunization of newbone testis supernatant from males of the same strain. ELISA test was used to identify the H-Y antibody of antiserum. Total 124 mouse embryos (8-cell stage) were treated with H-Y antiserum and complement in BSA free Ho, pp. and Pitt's medium and cultured under the gas phase of 5% CO2 in air at 37$^{\circ}C$ for 24 to 48 hrs. The morphological characteristics of embryos treated were observed under the phase-contrast micro scope. The results obtained in these experiments were summarized as follows: 1. Optimal Density of H-Y antibody were a, pp.ared to be 0.27-0.47 by ELISA test. 2. Of total 124 embryos treated with H-Y antiserum and complement 69(55.6%) embryos developed to blastocyst and 55(44.4%) destroyed or arrested.

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맥동압력조건에서 재생기를 통한 왕복유동의 압력강하 특성에 대한 연구 (Investigation on the pressure drop characteristics of oscillating flow through regenerator under pulsating pressure conditions)

  • 최성열;남관우;정상권
    • 한국초전도저온공학회:학술대회논문집
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    • 한국초전도저온공학회 2003년도 학술대회 논문집
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    • pp.21-27
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    • 2003
  • This paper proposes a new model of the pressure drop for more accurate description of oscillating flow through regenerator under pulsating pressure conditions in contrast to an existing model based on steady flow. For the universal uses of the oscillating flow model, non-dimensional parameters, which consist of Reynolds number, Valensi number gas domain length ratio, oscillating flow friction factor and phase angle of pressure drop, are derived from the capillary tube model of the regenerator. Two correlation equations of the model are obtained from the experiments for the twill square screen regenerators under various operating frequencies and inlet mass flow rates. The oscillating friction factor is a function of only the Reynolds number and the phase angle of pressure drop is a function of the Valensi number and the gas domain length ratio. Experiment is also performed to examine the effects of the shape of screens.

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Ultrafine Grained Steels Processed by Equal Channel Angular Pressing

  • Shin, Dong Hyuk
    • Corrosion Science and Technology
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    • 제5권1호
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    • pp.23-26
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    • 2006
  • Recent development of ultrafine grained (UFG) low carbon steels by using equal channel angular pressing (ECAP) and their room temperature tensile properties are reviewed, focusing on the strategies overcoming their inherent mechanical drawbacks. In addition to ferrite grain refinement, when proper post heat treatments are imposed, carbon atom dissolution from pearlitic cementite during ECAP can be utilized for microstructural modification such as uniform distribution of nano-sized cementite particles or microalloying element carbides inside UFG ferrite grains and fabrication of UFG ferrite/martensite dual phase steel. The utilization of nano-sized particles is effective on improving thermal stability of UFG low carbon ferrite/pearlite steel but less effective on improving its tensile properties. By contrast, UFG ferrite/martensite dual phase steel exhibits an excellent combination of ultrahigh strength, large uniform elongation and extensive strain hardenability.

Growth Behavior and Mechanisms in Cemented Carbides

  • Yoon, Byung-Kwon;Kang, Suk-Joong L.
    • 한국분말야금학회:학술대회논문집
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    • 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part2
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    • pp.891-892
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    • 2006
  • To test the correlation between grain shape and growth behavior we prepared WC-TiC-Co samples with rounded (Ti, W)C grains and faceted WC grains. The growth of rounded (Ti, W)C grains was normal. In contrast, the growth of faceted WC grains was abnormal or suppressed depending on the initial size of WC particles. These observations were explained using growth theories of crystals in a liquid and were also confirmed by a simulation using their growth equations. The present results thus demonstrate that the growth behavior of carbide grains in a liquid is governed only by their shape, irrespective of the presence of another phase.

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Y-TZP에서 표면 처리에 따른 저온열화 거동의 직접적 관찰 (Direct Observation on the Low Temperature Degradation Due to Surface Treatment in Y-TZP)

  • 정태주;김혜성
    • 한국분말재료학회지
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    • 제17권3호
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    • pp.197-202
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    • 2010
  • Low temperature degradation behavior in yttria stabilized tetragonal zirconia polycrystal (Y-TZP) ceramics was microscopically observed from the phase contrast between monoclinic surface and tetragonal matrix. The degradation behavior was dependent on the surface treatment of sintered Y-TZP, even if the sintering history is same. In the mirror polished specimen, the monoclinic layer appeared in a uniform thickness from the surface. On the contrary, for the specimen with coarse scratch, the thickness of degraded surface was more than double especially from the coarse scratch. Since the scratch results in local deformation, the residual stress should be induced around the scratch. With the transformation from tetragonal to monoclinic, the volume expansion exerts a stress on a neighboring grains and promotes a successive phase transformation. Such a autocatalytic effect can be triggered from the part of coarse scratch.

위상 변조기와 분산 광섬유를 이용한 광펄스 압축 (Optical pulse compression using a phase modulator and a dispersive optical fiber)

  • 명승일;한상진;서동선;최영완;박재동;주무정
    • 한국광학회지
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    • 제10권3호
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    • pp.243-247
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    • 1999
  • 정현파로 구동되는 위상변조기로 주파수 첩이 유기된 펄스를 발생시키고, 이 첩이 유기된 펄스를 분산이 있는 광섬유로 압축하는, 첩된 펄스의 압축에 의한 고속의 선천적으로 안정된 변환 제한급 광펄스 발생에 대해 보고한다. 실험결과 ∼14 피코초의 시간 대역곱이 ∼0.34인 Sech2 형태의 펄스를 10 GHz의 반복율로 성공적으로 발생시킬 수 있음을 보였다. 본 접근 방법은 고차 솔리톤 효과를 이용한 펄스 압축과 같은 여타 방법과는 다르게 광전력에 무관하기 때문에, 저 전력 레이저에 아주 유용하게 응용될 수 있다.

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단상 전류원형 능동전력필터를 위한 펄스폭 변조법의 개선 (An Improvement of PWM Method for Single Phase Current Source Type Active Power Filter)

  • 박인규;박종근
    • 대한전기학회논문지
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    • 제39권3호
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    • pp.254-262
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    • 1990
  • In the active power filter, the PWM method for controlling the power converter plays an important role for the performance and the efficiency of the filter. In this paper, a new PWM method for the single phase current source type active power filter is presented, which is intended particularly for obtaining the maximum efficiency of the compensation. This method is basically based upon the optimized injection method, but in contrast to the existing method, this method adapts the optimized injection method by putting not only the pulse width, but also the pulse position into control variables. By doing this, when the number of pulses per one cycle is N, lower order harmonics up to the Nth order can be completely eliminated by the filter. So, this method is much more efficient than any other existing method. These characteristics are well verified by the computer simulations and the experiments.

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Ti-Ni계 합금분말의 미세조직 및 상변태거동에 미치는 밀링조건의 영향 (The Effect of Milling Conditions on Microstructure and Phase Transformation Behavior of Ti-Ni Based Alloy Powders)

  • 강상호;남태현
    • 한국분말재료학회지
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    • 제8권1호
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    • pp.42-49
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    • 2001
  • Ti-50Ni(at%) and Ti-40Ni-10Cu(at%) alloy powders have been fabricated by ball milling method, and their microstructure and phase transformation behavior were investigated by means of scanning electron microscopy/energy dispersive spectrometry, differential scanning calorimetry (DSC), X-ray diffractions and transmission electron microscopy. In order to investigate the effect of ball milling conditions on transformation behavior, ball milling speed and time were varied. Ti-50Ni alloy powders fabricated with the milling speed more than 250 rpm were amorphous, while those done with the milling speed of 100rpm were crystalline. In contrast to Ti-50Ni alloy powders, Ti-40Ni-10Cu alloy powders were crystalline, irrespective of ball milling conditions. DSC peaks corresponding to martensitic transformation were almost discernable in alloy powders fabricated with the milling speed more than 250 rpm, while those were seen clearly in alloy powders fabricated with the milling speed of 100 rpm. This was attributed to the fact that a strain energy introduced during ball milling suppressed martensitic transformation.

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