• KSBB Journal
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• v.11 no.4
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• pp.445-452
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• 1996

To investigate the promoter effect on heterologous gene expression in S. cerevisiae, the recombinant plasmids pYI11, pYI12, pYI10-2, and pYIGP were constructed to contain the inulinase gene (INUI) as a reporter under the control of GAL10, GAL7, GAL1, and GAP promoters, respectively. When the yeasts transformants were cultivated on galactose-containing rich media, the cell growth reached to 36-39 OD600 at 72 hours of cultivation. The specific growth rates of the cells harboring the four different plasmids decreased similarly : they dropped from $0.24 h^{-1}$ during the glucose-consuming period to 0.04 -$0.10 h^{-1}$ during the galactose-consuming period (gene expression phase for GAL promoter system). After the depletion of glucose, the expression of inulinase gene was started and reached to maximal levels of 4.3(GAL1 promoter), 4.0(GAL10 promoter), 3.8(GAL7 promoter), and 1.6(GAP promoter) unit/mL at 72 hours of cultivation. Based on the maximal expression level and activity staining on the plate, the promoter strength was in the order of GAL1, GAL10, GAL7 and GAP promoter. While the GAL-promoter systems showed a high plasmid stabilities of more than 78%, the GAP-promoter plasmid revealed a lower plasmid stability of 55%. Most of inulinase activity (98%) was found in the extracellular medium, indicating that the secretion efficiency of inulinase is independent on the type of promoter.

• Journal of the Korean Society for Nondestructive Testing
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• v.33 no.5
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• pp.465-471
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• 2013

A steam generator in nuclear power plant is a heatexchager which is used to convert water into steam from heat produced in a nuclear reactor core, and the steam produced in steam generator is delivered to the turbine to generate electricity. Because of damage to steam generator tubing may impair its ability to adequately perform required safety functions in terms of both structural integrity and leakage integrity, eddy current testing is periodically performed to evaluate the integrity of tubes in steam generator. This assessment is normally performed during a reactor refueling outage. Currently, the eddy current testing for steam generator of nuclear power plant in Korea is performed in accordance with KEPIC & ASME Code requirements, the eddy current testing system is consists of remote data acquisition unit and data analysis program to evaluate the acquired data. The KEPIC & ASME Code require that the electrical properties of remote data acquisition unit, such as total harmonic distortion, input & output impedance, amplifier linearity & stability, phase linearity, bandwidth & demodulation filter response, analog-to-digital conversion, and channel crosstalk shall be measured in accordance with the KEPIC & ASME Code requirements. In this paper, the measurement requirements of electrical properties for eddy current testing instrument described in KEPIC & ASME Code are presented, and the measurement results of newly developed eddy current testing instrument by KHNP(Korea Hydro & Nuclear Power Co., LTD) are presented.

• Applied Chemistry for Engineering
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• v.26 no.6
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• pp.698-705
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• 2015

Two series of symmetric dimesogenic compounds containing a butylene or 1-methylbutylene spacer as a flexible group were synthesized. The mesogenic groups of synthesized compounds consist of an azobenzene group with a terminal substituent. Chemical structures as well as, thermal, mesomorphic, and photochemical properties of the synthesized compounds were investigated using FT-IR, $^1H-NMR$, differential scanning calorimetry (DSC), polarizing optical microscopy (POM), and UV-visible spectrometry. P-H, P-F, and $P-OC_6H_{13}$ showed monotropic liquid crystal phases, whereas the others showed enantiotropic liquid crystal phases. Compounds with butylene group as a flexible spacer exhibited wider mesophase temperature ranges and higher thermal transition temperatures than compounds containing a 1-methylbutylene group. Compounds with a high absolute value of the Hammett substituent constant exhibited high thermal transition temperatures and improved stability in the liquid crystal phase. Furthermore, in the absence or presence of UV light illumination, terminal substituents of the azomesogenic group were important factors in deciding the maximum absorbance wavelength (${\lambda}_{max}$) and the rate of photoisomerization (K).

• Applied Chemistry for Engineering
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• v.24 no.5
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• pp.443-455
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• 2013

Electrochemical studies on charge transfer reactions across the interface between two immiscible electrolyte solutions (ITIES) have greatly attracted researcher's attentions due to their wide applicability in research fields such as ion sensing and biosensing, modeling of biomembranes, pharmacokinetics, phase-transfer catalysis, fuel generation and solar energy conversion. In particular, there have been extensive efforts made on developing sensing platforms for ionic species and biomolecules via gelifying one of the liquid phases to improve mechanical stability in addition to creating microscale interfaces to reduce ohmic loss. In this review, we will mainly discuss on the basic principles, applications and future aspects of various sensing platforms utilizing ion transfer reactions across the ITIES. The ITIES is classified into four types : (i) a conventional liquid/liquid interface, (ii) a micropipette supported liquid/liquid interface, (iii) a single microhole or an array of microholes supported liquid/ liquid interface on a thin polymer film, and (iv) a microhole array liquid/liquid interface on a silicon membrane. Research efforts on developing ion selective sensors for water pollutants as well as biomolecule sensors will be highlighted based on the use of direct and assisted ion transfer reactions across these different ITIES configurations.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.4
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• pp.7-13
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• 2012

The performance of blind equalization algorithm ISCA was compared with MSCA that is used for the minimization of the inter symbol interference which occurs in the time dispersive communication channel for digital transmission. Because of the non-linearities of a magnitude and phase transfer characteristics of a communication channel, the transmitting signal will be received that band limited and time dispersived. Therefore the distortion was compensated by using the self adaptive equalizer at the receiving side, then passing through the detector for the decision of "1" or "0". At this time the Constellation Dependent Constant is played an important role in the adaptive equalizer used on the receiver. In order to calculation of this constant, the ISCA and MSCA was used the second order statistics. The ISCA and MSCA which are possible to compensation of mensioned transfer function simulataneously, are improved the performance of original SCA algorithm and then was compared the performance by computer simulation. For this, the recovered constellation, residual isi and MSE was used, and a result of performance comparison, the ISCA algorithm has better than the MSCA in every performance index. But on the steady state of equalizer, the variation of performance due to the CME terms in the MSCA equalization algorithm was less than the ISCA, so MSCA has better stability.

• Journal of Energy Engineering
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• v.25 no.4
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• pp.176-183
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• 2016

This study relates to a polymer electrolyte membrane for improved performance fuel cell, were researched with respect to properties required for driving a fuel cell. The bis(4-fluorophenyl)phenyl phosphine oxide was sulfonated using fuming sulfuric acid. Synthetic hydrophilic oligomer and the hydrophobic oligomer and the block copolymers were prepared via aromatic nucleophilic substitution polycondensation. A block copolymer structure and degree of sulfonation was analyzed by $^1H$-NMR and gel permeation chromatography(GPC) analysis. Thermal stability was confirmed by thermogravimetric analysis(TGA), block copolymer was stable at high temperature(>$200^{\circ}C$), The ion conductivity was measured in order to demonstrate the performance of fuel cell. Synthesis membrane was the increase of temperature was improved conductivity up to 58 mS/cm due to the influence of the developed ion clusters. The phase separation of the polymer was observed to make AFM analysis.

• The Journal of Engineering Geology
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• v.17 no.2 s.52
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• pp.187-196
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• 2007

The Dalseong acid mine drainage were studied focused on the characters of schwertmannite that controls geochemistry of the stream. Besides chemical analysis of stream water, particle size analysis, XRD SEM and TEM were performed on precipitates of streams and on wasted metalliferous ores. The AMD discharged from the abandoned mine reveals a decrease of pH and EC downward stream. Euhedral sulfur occurs as equigranular aggregates on the altered pyrite while fine acicula goethite coalesces to form cross, star, or starfish-like shapes. Water chemistry plotted on the Eh-pH diagram shows that schwertmannite and ferrihydrite are stable phases. Reddish brown precipitates consist of mostly schwertmannite with less goethite, whereas yellowish brown precipitates are composed of geothite with less schwertmannite. The particle size of precipitates ranges $d(0.1)\;0.861{\mu}m{\sim}3.769{\mu}m,\;d(0.5)\;3.984{\mu}m{\sim}15.255{\mu}m,\;and\;d(0.9)\;9.875{\mu}m{\sim}56.726{\mu}m$. Schwertmannite is characterized by equigranular spheric form. Pincushion or spicule with 100nm width and $200{\sim}300nm$length form on schwertmannite sphere with radial growth patterns. It is highly probable that reddish or yellowish brown precipitates formed in many AMDs may contain schwerhnannite. Because it can serve as sink for removing heavy elements by adsorption in AMD system, there is a need to correctly identify schwertmannite in precipitates and to characterize its phase stability.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.10 no.3
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• pp.271-277
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• 2000

Transparent $TiO_2$ thin films were prepared by the sol-gel method from titanium alkoxide aqueous solution containing large quantities of water. To prepare the aqueous system sol, the chelate compound solution were prepared from each 1 mole of titanium(IV)iso-propoxide, acetylacetone and 8 moles of isopropyl-alcohol. And then the 50 moles aqueous solution with the 0.02~0.50 moles of HCI for sol stability and the 0.3 mole of polyethylene glycol for coating adhesion were precisely dropped on the chelate compound solution. $TiO_2$thin films were fabricated by dip coating, drying and heat treatment at $400^{\circ}C$ and their characteristics were investigated by XRD, SEM and UV-visible spectrometer, The neutral sol of composition with TTIP : AcAc : IPA : $H_2O$ : PEG : HCl = 1 : 1 : 8 : 50 : 0.3 : 0.15 (molar ratio) was maintained stable sol condition and without problem for coating more than 30 days. The anatase phase $TiO_2$thin film prepared from 30 times dipping, drying and heat treating showed the flat surface and the fine microstructure of fracture surface with about 2 $\mu\textrm{m}$ thickness. Transparency of these $TiO_2$thin film was 80 % in the visible region.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.27 no.9
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• pp.854-864
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• 2016

The use of small drone platform has become a popular topic in these days but its application for SAR operation has been little known due to the burden of the payload implementation. Drone platforms are distinguished from the conventional UAV system by the increased vulnerability to the turbulences, control-errors and poor motion stability. Consequently, sophisticated motion compensation may be required to guarantee the successful acquisition of high quality SAR imagery. Extremely limited power and mass budgets may prevent the use of additional hardwares for motion compensation and the difficulty of SAR focusing is further aggravated. In this paper, we have carried out a feasibility study of mico-SAR drone operation. We present the image acquisition results from the preliminary flight tests and a quality assessment is followed on the experimental SAR images. The in-flight motion errors derived from the unique drone movements are investigated and attempts have been made to compensate for the geometrical and phase errors caused by motions against the nominal trajectory. Finally, the successful operation of drone SAR system is validated through the focussed SAR images taken over test sites.

• Journal of the Korean Geotechnical Society
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• v.30 no.9
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• pp.57-66
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• 2014

The ground in cold region consists of active and permafrost layers. The active layer at the unstable state may cause ground corrosion and uplift, when the temperature of frozen ground increases due to seasonal changes. The thermosyphon is one of the stabilization methods to maintain the ground stability in the frozen ground. The thermosyphon is a closed two-phase convection device that extracts heat from the ground and discharges it into the atmosphere. In this study, ground freezing experiment using a thermosyphon and simulated ground with the isolation material was conducted to evaluate the thermal performance of the thermosyphon. In order to consider the thermal performance of the thermosyphon, commercial numerical program (TEMP/W) was adopted. Likewise, the thermal performance of thermosyphon and thermal properties of ground were applied in the numerical model. In a series of comparisons with experiment results and numerical study, thermal performance of thermosyphon can be evaluated.