• 한국식품위생안전성학회지
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• 제16권2호
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• pp.139-144
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• 2001

밀과 보리의 방사선 조사 여부를 검지하기 위해 생물학적 방법인 발아법을 이용하였다. 곡류의 순과 뿌리 성장은 0.3 kGy에서도 상당히 억제되므로 0.5 kGy 이하의 저선량으로 감마선 조사하였고 순과 뿌리 길이를 측정하여 성장정도를 확인하였다. 밀의 순은 모든 선량에서 5일 동안 지속적인 성장을 하였지만 0.1 kGy조사시료를 제외하고 조사선량이 증가할수록 길이가 짧아지거나 성장정도가 감소하는 경향을 보여 비조사 시료와 조사 시료의 구분이 가능하였다. 밀의 뿌리는 비조사 시료의 길이 성장정도가 조사 시료들보다 컸으며 3일째 뿌리의 길이가 20mm에 가장가깝게 성장하였으므로 이를 기준하여 조사여부를 판정하였다. 보리의 경우 순과 뿌리 모두 조사선량이 증가할수록 성장이 지연되었다. 순은 0.3 kGy 이상 조사 시료에서 조사선량이 증가할수록 5일간의 성장정도가 점차 감소하여 비조사 시료와 조사시료의 구분이 가능하였고 보리의 뿌리는 3일부터 조사 시료들과 비조사 시료들간에 차이를 보이면서 5일째에는 0.3 kGy 이상 조사 시료들과 비조사 시료들간에 통계적으로 유의한 차이를 보였다. 보리의 방사선 조사 여부의 판별은 뿌리가 3일 째 20mm에 가장 가깝게 성장한 것과 5일 동안 성장정도가 가장 큰 것을 기준하여 비조사 시료와 조사 시료를 판별할 수 있었다.

• Restorative Dentistry and Endodontics
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• 제16권2호
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• pp.99-117
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• 1991

The purpose of this study is to evaluate the effects of dentin bonding agents on the fibroblasts cultivated from human pulp tissue. The fibroblasts were cultured in DMEM/10%FBS medium. Whatman filter paper discs (6mm diameter) soaked with $2{\mu}l$ of dentin bonding agents were placed on a millipore filter (pore size $0.22{\mu}m$) contained in a 50mm Petri dish, and then, exposed for 10 minutes, 30 minutes, 1 hour, 6 hours, 12 hours, 24 hours, 4 days and 7 days in $37.^{\circ}C$, 5% $CO_2$ incubator. The results of the experiments were analyzed by counting the cells and measuring the protein contents at 1 day, 4 days and 7 days. The results of this study were as follows: l. CLEARFIL NEW BOND, LITE-FIL BOND, GLUMA 3 Primer and GLUMA 4 Sealer showed cytotoxicity compared to the control group in the cell counts and the protein contents. 2. GLUMA 4 Sealer showed the least cytotoxicity among the three dentin bonding agents. 3. The results of the cell count were simialr to the results of protein content measurement. 4. LITE-FIL BOND exhibited marked cytotoxicity during 1 day, but, the cytotoxicity was slightly reduced after 4 and 7 days. 5. In GLUMA 3 Primer group, it was not possible to count the cell numbers and measure the protein contents, but the degeneration of cells was observed under the inverted phase-contrast microscope.

• Journal of Biosystems Engineering
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• 제39권4호
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• pp.318-323
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• 2014

Purpose: This study was carried out to predict the growth period and fresh weight of sprouts grown in a cultivator designed to grow sprouts under optimal conditions. Methods: The temperature, light intensity, and amount of irrigation were controlled, and images of seed sprouts were acquired to predict the days of growth and weight from pixel counts of leaf area. Broccoli, clover, and radish sprouts were selected, and each sprout was cultivated in a 90-mm-diameter Petri dish under the same cultivating conditions. An image of each sprout was taken every 24 hours from the 4th day, and the whole cultivating period was 6 days, including 3 days in the dark. Images were processed by histogram inspection, binary images, image erosion, image dilation, and the overlay image process. The RGB range and ratio of leaves were adjusted to calculate the pixel counts for leaf area. Results: The correlation coefficients between the pixel count of leaf area and the growth period of sprouts were 0.91, 0.98, and 0.97 for broccoli, clover, and radish, respectively. Further, the correlation coefficients between the pixel count of leaf area and fresh weight were 0.90 for broccoli, 0.87 for clover, and 0.95 for radish. Conclusions: On the basis of these results, we suggest that the simple image acquisition system and processing algorithm can feasibly estimate the growth period and fresh weight of seed sprouts.

• 대한의용생체공학회:의공학회지
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• 제38권6호
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• pp.308-312
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• 2017

Skeletal muscle function plays a very important role in quality of life. However, skeletal muscle causes functional decline under aging or some diseases. Exercise and muscle training are good solutions to delay sarcopenia, but there are limitations to those who are uncomfortable in exercise. For this reason, alternative interventions for muscle sarcopenia are required, and many studies proved the increase of skeletal muscle mass by electrical stimulation. In conventional studies, however, mouse skeletal muscle cells have been mostly used in experiments to identify electrical stimulation conditions while human derived cells have not been frequently utilized in these studies. Stimulation used for rehabilitation has been uniformly treated without the consideration of aging. In addition, many studies have been used with conventional petri dish usually requiring many numbers of cells, which is not appropriate for rare. Moreover, they are not usually condition uniformity of electrical field. In this study, we have developed an electrical stimulation device which consumes a small amount of cells and can form a uniform electrical field. With the system, we analyzed the skeletal muscle differentiation and Myotube thickness depending on the electrical stimulation condition.

• 한국토양동물학회지
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• 제8권1_2호
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• pp.17-22
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• 2003

국내에서 채집된 메기디스 곤충병원선충(Heterorhabditis megidis)을 이용하여 집파리(Musca domestica)와 풀색꽃무지(Gametis jucunda)에 대해서 방제 효과를 검정하였다. 페트리디시 실내실험에서 H. megidis는 집파리와 풀색꽃무지 2령충에 대해 각각 456.4 및 238.9 infective juveniles (IJs)/마리의 반수치사 농도를 보였다. 이는 이미 방제 효과가 잘 알려진 곤충병원선충인 Steinernema carpocapsae을 동일한 두 종에 처리하였을 때 얻은 반수치사 농도(집파리는 115.9 IJs/마리, 풀색꽃무지는 388.6 IJs/마리)와 대조되었다. 포장시험에서는 집파리가 발생하는 축산농가에 평방 미터당 1,000,000 IJs를 처리할 경우 56.9%의 방제 효과를 보였다. 반면에 풀색꽃무지가 발생되는 사과원에 평방 미터당 370,000 IJs를 처리할 경우 57.3%의 방제 효과를 보였다. 이러한 결과는 국내에서 발생하는 메기디스 곤충병원선충이 집파리와 풀색꽃무지에 대해서 효과적 생물방제 인자가 될 수 있는 것을 제시한다.

• Journal of Periodontal and Implant Science
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• 제25권2호
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• pp.210-221
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• 1995

The purpose of this study was performed to investigate the mineralization and differentiation of osteobalsts for bone regeneration in vitro and the effect of rate of the composition in periodontal cells on mineralization. For this study, healthy gingival tissues were surgically obtained from the patients during 1st premolar extraction for the purposes of orthodontic treament. Gingival tissue was washed several time with Phosphate buffered saline contained high concentration of antibiotics and antifungal agent, and cultured in Dulbecco's Modified Eagle's Medium(DMEM, Gibco, U.S.A.). Every cell were cultured in state at $37^{\circ}C$, 100% of humidity, 5% of $CO_2$ incubator. Bone marrow stromal cells were isolated from 5-clay-old rat femur with using medium irrigation mathod by syringe. Cell suspension medium were centrifuged at 1500 rpm for 5 min and then cultured in the petri dish. Two kinds of cell were freezed and stocked in the liquid nitrogen tank until experiment. Cell were incubated into the 24 multi-well plate with $5{\times}10^4$cell/well of medium at $37^{\circ}C$, 100% of humidity 5% $CO_2$ incubator for 24 hours. After discarded of the supernatent of medium, O.5ml of medium were reapplied and incubated. And counted the number of cell using the hemocytometer and inverted light microscope. We have measured the number of mineralized nodule with using Alizarin red S. staining in microscope. Furthermore every cell were observed the morphological change between every rate of co-culture of the two kinds of cell. The results were as follows; The rate of proliferation of co-culture cell revealed high rate tendency compared the bone marrow stromal cell only and low growth rate to compared with gingival fibroblast only. The tendency of formation of the mineralized nodule were observed dose-depend pattern of bone marrow stromal cell. It is concluded that the gingival fibroblast may inhibit the formation of mineralized nodule in the culture of the bone marrow stromal cell.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.200-200
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• 2017

Salinity stress is one of the most important abiotic stresses and severely impairs plant growth and production. Root is the first site for nutrient accumulation like as $Na^+$ in the plant. To investigate the response of wheat root under salinity stress, we executed the characterization of morphology and analysis of metabolites. Wheat seeds cv. Keumgang (Korean cultivar) were grown on the moist filter paper in Petri dish. After 5 days, seedlings were transferred to hydroponic apparatus at 1500 LUX light intensity, at $20^{\circ}C$ with 70% relative humidity in a growth chamber. Seedlings (5-day-old) were exposed to 50mM, 75mM, 100mM NaCl for 5 days. Ten-day-old seedlings were used for morphological characterization and metabolite analysis. Root and leaf length became shorter in high NaCl concentration compared to following NaCl treatment. For confirmation of salt accumulation, wheat roots were stained with $CoroNa^+$ Green AM, and fluoresce, and the image was taken by confocal microscopy. $Na^+$ ion accumulation rate was higher at 100mM compared to the untreated sample. Furthermore, to analyze metabolites in the wheat root, samples were extracted by $D_2O$ solvent, and extracted sample was analyzed by 1H NMR spectroscopy. Fourteen metabolites were identified in wheat roots using NMR spectroscopy. Methanol and ethanol were up-regulated, whereas formate, aspartate, aminobutyrate, acetate and valine were down-regulated under salinity stress on roots of wheat. Fumarate had no change, while glucose, betaine, choline, glutamate and lactate were unevenly affected during salinity stress.

• Korean Chemical Engineering Research
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• 제51권6호
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• pp.760-765
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• 2013

액적기반 미세유체 시스템을 이용해 난백단백질인 라이소자임의 결정화실험을 하였다. Flow-focusing 칩을 이용해 water-in-oil 형태의 액적을 만들고 페트리 디쉬와 십자몰드에 넣은 후, 액적 내부에서 라이소자임 수용액과 침전제 (NaCl) 사이의 액-액 반응을 관찰하였다. 그리고 수용액의 pH가 4.8일 때와 7.2일 때의 결정형태를 비교하였다. 그 결과, pH 4.8에서는 다면체 또는 판상형의 결정이 형성되었고, pH 7.2에서는 침상형 결정이 생성되었다. pH 4.8, 7.2 두 경우 액적이 홀로 있을 때에는 액적부피가 유지되거나 감소하면서 결정이 형성되었다. 하지만 액적이 서로 인접해 있을 때는 액적사이의 상호작용이 관찰되었고, 두 pH에서 다른 경향성을 보였다. pH 4.8에서는 인접한 액적의 부피에 영향을 주어 한 액적의 부피가 커졌고, 부피가 커진 액적에서 결정이 형성되었다. pH 7.2에서는 부피에 영향을 서로 주지 않고 각각의 액적에서 결정이 형성되었다.

• Asian Journal of Atmospheric Environment
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• 제5권1호
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• pp.21-28
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• 2011

Occupational nanomaterial exposure is an important issue in the manufacture of such products. People are also exposed to various nanoparticles in their living environments. In this study, we investigated nanoparticle formation during the reaction of ozone and volatile organic compounds (VOCs) emitted from a commercial air freshener, one of many widely used consumer products, in a $1-m^3$ reaction chamber. The air freshener contained various VOCs, particularly terpenes. A petri dish containing 0.5 mL of the air freshener specimen was placed in the bottom of the chamber, and ozone was continuously injected into the center of the chamber at a flow rate of 4 L/min with an ozone concentration of either 50, 100 or 200 ppb. Each test was conducted over a period of about 4 h. The higher ozone concentrations produced larger secondary nanoparticles at a faster rate. The amount of ozone reacted was highly correlated with the amount of aerosol formation. Ratios of reacted ozone concentration and of formed particle mass concentration for the three injected ozone concentrations of 50, 100 and 200 ppb were similar to one other; 4.6 : 1.9 : 1.0 and 4.7 : 2.2 : 1.0 for ozone and aerosol mass, respectively.

• 대한수의학회지
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• 제30권4호
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• pp.487-497
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• 1990

This study was carried out to observe in vitro effect of praziquantel on the tegumental changes of Heterophyopsis continua with scanning electron microscope. Metacercariae were collected from the perch, Lateolabrax japonicus, by artificial digestion technique and fed to 2-week old chickens. Adult worms were recovered from small intestine of chickens 8 days after infection. For working solutions, praziquantel was diluted with TC199 medium at concentration of 0.01, 0.1. 1 and $10{\mu}g/ml$. To each petri dish containing 10ml of solution, 5~10 worms were introduced and incubated at $37^{\circ}C$. For the scanning electron microscopy(SEM), the worms were fixed in cold 2.5% glutaraldehyde, dehydrated in a series of graded ethanol and freeze-dried. Dried specimen was mounted on stub and coated with gold and observed in an SEM. The results were as follows: 1. Severe tegumental alterations were recognized by scanning electron microscope. Bleb formation of tegument was observed in 5 minute group and most pronounced on anterior tegument of worms. The number and size of blebs increased as incubation time prolonged. 2. The surface destruction was more pronounced at ventral margin between the oral and the ventral suckers. 3. The sensory papillae were slightly affected, but destruction of tegumental spine was not recognized. 4. The effect of praziquantel on the worm was found dependent on the concentration and incubation time, however, the effect was more dependent upon the incubation time.