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http://dx.doi.org/10.9713/kcer.2013.51.6.760

Lysozyme Crystallization in Droplet-based Microfluidic Device  

Ko, Kwan-Young (Department of Chemical Engineering, Chungnam National University)
Kim, In-Ho (Department of Chemical Engineering, Chungnam National University)
Publication Information
Korean Chemical Engineering Research / v.51, no.6, 2013 , pp. 760-765 More about this Journal
Abstract
Lysozyme crystallization was performed by using flow-focusing chip in droplet-based microfluidic system. Water-in-oil droplets were formed in the system and collected on petri-dish and cross type mold. Liquid-liquid reaction of lysozyme and sodium chloride occurred in the droplet and crystals were observed through microscope. Solution pH was varied as 4.8 and 7.2. Crystals of polyhedron and plate-like shape were obtained at pH 4.8, while needle structure crystals formed at pH 7.2. Lysozyme in single droplet for two pHs were crystallized with constant or decreased droplet size. However, crystals at pH 4.8 were only obtained in the droplet of which size was increased by the interaction between droplets. Droplet volume did not change at pH 7.2 and crystals formed in both droplets.
Keywords
Microfluidic System; Droplet; Lysozyme; Protein Crystallization;
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Times Cited By KSCI : 4  (Citation Analysis)
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