• 한국육종학회지
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• 제42권5호
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• pp.431-438
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• 2010

Anthers of sweet pepper $F_1$ cultivar 'Special' were cultured on Dumas De Vaulx (C medium), supplemented with $0.1mgL^{-1}$ 2, 4-D and $0.1mg{\cdot}L^{-1}$ kinetin with 3% sucrose, and 0.32% phytagel. The calluses obtained were further sub-cultured on Murashige and Skoog (MS) medium without growth regulators for regeneration. Regenerated plantlets were grown in plastic pots under plastic house and characterized their cytological and morphological characters in spring, 2008. Twenty percent plantlets were identified as haploid plants after chromosome and ploidy analysis. Haploid plants contained 12 chromosomes, high stomatal density with small stomatal length as compared to diploid plants. Stomatal length in haploids was 23.3% smaller than diploids. Haploid plants were characterized as small leaf and petiole size, poor vigor, thin stem and short plant height, short internodes and small flower buds, fruit size and fruit weight as compared to diploid plants and most of the haploid fruits were seedless. SP55, SP62, SP68, SP72 and SP77 are found high yielding double haploids with high total soluble content (8.6, 8.7, 9.2, 9.1 and $9.8^{\circ}Brix$, respectively) and desirable fruit shape, and recommended them to exploit as inbred lines for heterosis breeding.

• 식물분류학회지
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• 제49권1호
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• pp.1-7
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• 2019

Early leaves within the Viola albida complex were investigated by scanning electron microscopy in order to determine the morphological segments during morphogenesis. The early leaf development of V. albida var. albida could be morphologically divided into the eight stages in the following order: I, the initiation of shoot germination; II, the conical growth directionally of the leaf; III, the adaxial and abaxial formation of the leaf; IV, the initiation of the stipule; V, the formation of a transitional zone between the leaf blade and petiole; VI, the expansion of the upper part of the leaf blade; VII, the formation of almost all parts of the early leaf; VIII, the early simple leaf. Viola albida var. takahashii differs from V. albida var. albida by additional stages, i.e., V-1, the initiation of the first lateral lobe at the both lateral parts of the leaf after the stage V and an early lobed leaf. Viola albida var. chaerophylloides is also distinguished from two taxa by two developmental features, V-2, the initiation of a second lateral lobe below of the first lateral lobe, and an early palmately compound leaf. These findings suggest that the Viola albida complex would be in the process of peramorphosis, showing developmental changes in a chain of events, leading to a different leaf shape. These data would also be useful for isolating genes that give rise to different leaf morphogenesis outcomes among the taxa in the Viola albida complex.

• 한국작물학회지
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• 제25권3호
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• pp.63-67
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• 1980

인삼의 재래종에 대해서 유용형질과 관계가 있는 가시 Marker gene을 탐색하고, 선발을 용역하게 할수 있는 육종의 기초자료를 얻고저 년생별 각종 농경형질간의 상관관계를 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. 형질상호간의 상관관계는 년생에 따라 다소 차이가 있었으나 경직경, 엽중, 경중은 각년생 공히 거의 모든 형질과 고도의 유의성이 있는 정(+) 상관을 보였다. 2. 각년생차에 관계없이 수량형질인 근중과 상관이 높았던 지상부형질은 경직경, 엽중, 엽폭, 장엽수, 소엽수, 엽중, 경중이었으며, 이들은 모두 정(+)상관을 보였다. 3. 따라서 경직경이 굵고, 엽면적이 넓고, 엽수가 많은 것이 다수성일 것으로 사료됨으로 경직경과 엽면적 및 엽수를 표식형질로 활용, 선발하는 것이 효과적일 것으로 생각된다.

• 식물병연구
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• 제29권1호
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• pp.82-87
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• 2023

Exogenous ferrous chloride (FeCl₂) suppressed in vitro growth of Ralstonia pseudosolanacearum, causing bacteria for tomato bacterial wilt. More than 50 μM of FeCl₂ reduced the in vitro bacterial growth in dosedependent manners. Two to 200 μM of FeCl₂ did not affect the fresh weight of detached tomato leaves at 3 and 5 days after the petiole dipping without the bacterial inoculation. The bacterial wilt of the detached tomato leaves was evaluated by inoculating two different inoculum densities of R. pseudosolanacearum (10⁵ and 10⁷ cfu/ml) in the presence of FeCl₂. Bacterial wilt in the detached leaves by 10⁵ cfu/ml was efficiently attenuated by 10-200 μM of FeCl₂ at 3 and 5 days post-inoculation (dpi), but bacterial wilt by 10⁷ cfu/ml was only reduced by 200 μM of FeCl₂ at 3 and 5 dpi. These results suggest that iron nutrients can be included in the integrated disease management of tomato bacterial wilt.

• Journal of Plant Biotechnology
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• 제1권2호
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• pp.91-95
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• 1999

Genetic engineering of stone fruit species like apricot, plum, peach and cherry are hampered by the inefficient and low-level regeneration processes in tissue culture. The first transgenic stone fruit species have emerged from transformed hypocotyls. These great achievements were applauded by the scientific community contrary the fact that hypocotyl derived transgenic plants have no real brooding value. Tissue culture of different organs of valuable cultivars are recorded with an extremely low-level of regeneration in the literature. To improve the tissue culture basis of stone fruit plants an extensive tissue culture programme were launched and dozens of different media were compared including a series of hormone concentration in the tissue culture systems. Our continuous efforts were crowned by a very efficient method for achieving up to 30-40% regenerable petioles. Usually on a single petiole several well-separated meristems were induced. After 3-4 weeks of cultivation shoots were developed. The basic media $K_2$ were supplemented with 10g/L saccharose, 10g/L glucose and 10g/L maltose. The following plant hormones were used BAP 1mg/L, TDZ 1mg/L, 2-iP 1mg/L and IAA 0,1 mg/L concentrations. The Petri dishes were kept for 3 weeks in dark at a temperature 22$^{\circ}C$ for 8 hours and 22-24$^{\circ}C$ for 16 hours. The Petri dishes were sealed with Parafilm. The regeneration of the petioles were genotype independent and we were able to regenerate different plum cultivars with almost the same efficiency.

• 한국환경생태학회지
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• 제19권4호
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• pp.348-357
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• 2005

본 연구는 우리나라의 중요한 조경소재로 과도한 남획 및 서식지 변경으로 개체군이 급격히 파괴되어 위협에 처해있는 호랑가시나무를 대상으로 자생지와 자생지 인근의 식재지를 대상으로 잎의 형태적 특성을 분석하였다. 엽 형태변이의 분석결과, 엽장 $0.9cm{\~}11.8cm$, 엽폭 $0.4cm{\~}7.7cm$, 엽병장 $0.2mm{\~}13.0mm$, 엽 면적 $0.7cm^2{\~}46.7cm^2$ 및 결각수 $1{\~}13$개로 나타났다. 주로 잎의 모양, 결각의 수, 잎의 색 및 열매의 색등에 의해 구분하는 호랑가시나무의 재배품종을 자생지와 식재지에서 구분을 시도한 바, 절부암('Cholbuam'), 인천('Inchon'), 순천('Sun Chun')등 총9종류의 재배품종과 1종류의 교잡종으로 식별하였다.

• 한국환경과학회지
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• 제27권10호
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• pp.821-829
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• 2018

This study was conducted to examine the influence of Light-Emitting Diode (LED) light quality in urban agricultural plant factories on the growth and development of Seolhyang strawberry daughter plants in order to improve the efficiency of daughter plant growth and urban agriculture. LED light quality by demonstrated that above-ground growth and development were greatest for daughter plant 2. Daughter plant 1 showed the next highest growth and development, followed by daughter plant 3. Among the different qualities of LED light, the stem was thickest and growth rate of leaves was highest for R + B III (LED quality: red 660 nm + blue 450 nm/photosynthetic photon flux density (PPFD): $241-243{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and lowest for R (red $660nm/115-117{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). Plant height, leaf width, petiole length, and the leaf growth rate were highest for W (white fluorescent lamp/$241-243{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and lowest for R + B I (red 660nm+blue 450nm/$80-82{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). For above-ground growth and development, as the plants surpassed the seedling age, mixed light (red + blue), rather than monochromatic light (red or blue), and higher PPFD values tended to increase development. Regarding the quality of the LED light, daughter plant 2 showed the highest chlorophyll content, followed by daughter plant 1, and daughter plant 3 showed the least chlorophyll content. When the wavelength was monochromatic, chlorophyll content increased, compared to that when PPFD values were increased. Mixed light vitality was highest in daughter plant 2, followed by 1, and 3, showed increased photosynthesis when PPFD values were high with mixed light, in contrast to the results observed for chlorophyll content.

• 한국식품과학회지
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• 제23권4호
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• pp.420-427
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• 1991

배추의 염절임 및 가열처리 중의 조직구조 변화를 현미경적으로 관찰하고 펙틴질을 분별 정량하였으며 조직의 내부구조를 cytorrhysis로 측정하였다. 배추줄기 조직의 유세포의 세포막은 염절임시 수축 파괴되며 세포간 공간의 포집공기와 세포액은 세포수축과 함께 중엽을 파괴하면서 유출되었다. 배추조직의 펙틴은 주로고 메톡실 펙틴인 pA와 저 메톡실 펙틴인 pB로 구성 되었고 염절임 및 $60{\sim}70^{\circ}C$의 저온 가열시 상당량의 pA는 작용으로 pB로 전환되었고 $80^{\circ}C$ 이상으로 가열처리하면 열분해로 pB가 감소되었으며 열처리 중 조직의 firmness는 pB의 함량과 상관관계가 높았다(r=0.996). Cytorrhysis 실험결과 염절임 및 $60^{\circ}C$의 저온처리에서는 조직의 pore size가 더 작아졌고 $80^{\circ}C$ 이상의 고온 열처리에 의하여 조직의 pore size가 커졌는데 이는 저온에서 pB의 증가에 의해 조직이 더 치밀해지고 고온 열처리에서는 가열분해된 pB가 용출되면서 조직이 더 엉성해졌기 때문인 것으로 판단되었다.

• 한국작물학회지
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• 제47권5호
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• pp.356-360
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• 2002

The seedlings of yacon (Polymnia sonchifolia Poeppig ＆ Endlicher), which were cuttings, plug seedling (PS), crown bud before sprouting (CBBS), crown bud after sprouting (CBAS), and divided seedling after budding (DSAB) were planted at 70$\times$50 cm planting distance on ridge; that was interow spacingintrarow spacing, and about 28,500$\pm$71 plants/ha. CBBS didn't need work and equipment to raise seedlings. PS and DSAB grew taller to 140.5 and 143.3cm, respectively, than others at 150 days after planting. In the changes of plant height, PS and DSAB showed taller than others during growth period, cuttings, CBBS, and CBAS grew rapidly in middle growth stage. Excepting main stem and petiole length, other characters were significant for seedling. Fresh weights were different among seedlings. Even though the yield of plants grown from CBAS and CBBS were lower with 34.7 and 36.4 ton/ha, respectively, than 3.6 ton/ha of DSAB; its yield index were over 95%, hence, those of plants grown from cutting and PS were lower with 73 and 87%, respectively. The ratio of tuberous roots over 200g to total tuberous roots per plant was the highest from DSAB. Most of tuberous roots were under 200g per tuberous root from cuttings. CBBS, CBAS, and DSAB are suitable to use seedlings for high yield of yacono. Yacon plant by DSAB much produced tuberous root of over 200g.

• 원예과학기술지
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• 제17권1호
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• pp.23-28
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• 1999

Prunus cerasifera, P. domestica 및 P. salicina에서 기원된 자두 53 종 또는 품종으로 부터 27 형태형질의 특성을 조사하여 품종 동정에 필요한 기초자료를 제공하고, 그들간의 분류적 관계를 알아보기 위하여 주성분분석 및 집괴분석을 실시하였다. 조사된 형태형질 중 잎의 크기, 잎의 형태 및 엽내 털 발생정도 등이 자 두아속 식물의 동정 및 분류적 관계 해석에 있어서 상당히 유용하게 이용될 수 있을 것으로 단되었으며, 잎의 길이, 엽병 길이, 밀선수, 형, 엽저, 만개기 등은 P. domestica계 자두와 P. salicina계 자두간에 명확한 차이를 나타내었다. 또한 주성분분석 득점치를 이용한 비가 평균결합법에 의한 집괴분석 결과 53개 분류 군들은 평균거리 1.0을 기준으로 할 때 P. alicina-P. cerasifera 표현군, P. domestica 표현군 및 P. pinosa 표현군 등 3개의 표현군으로 분류될 수 있어, 분류군들의 대략적인 분류적 관계 분석이 가능하였다. 그러나 정확한 종군 분류를 위해서는 보다 많은 형태형질의 용이 필요할 것으로 판단되었다.