• 한국식품영양과학회지
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• 제25권4호
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• pp.588-592
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• 1996

미나리의 MeOH추출물에서 분획한 ethyl acetate 가용부를 silica gel column chromatography를 이용하여 isorhamnetin, hyperoside 및 persicarin의 플라보노이드 화합물을 분리하였으며, in vitro에서 Salmonella typhimurin TA 100균주를 이용하여 항돌연변이 활성을 관찰하였다. 이들 화합물 중 isorhamnetin은 0.5mg/plate에서 33%, 1.0mg/plate에서는 59%, persicarin은 0.5mg/plate에서 40%, 1.0mg/plate에서 47%의 돌연변이 저해효과를 나타내었다. 이와 유사한 플라보노이드 화합물로서 엉겅퀴 지상부에서 분리한 acacetin 7-rutinoside, cirsimaritin 4＇-glucoside 및 hispidulin 7-neohesperidoside에 대해서도 1mg/plate 농도에서 acacetin 7-rutinoside와 cirsimaritin 4＇-glucoside가 40% 및 49%의 돌연변이 억제활성이 관찰되었다.

• 생약학회지
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• 제39권2호
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• pp.142-145
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• 2008

Five compounds were isolated from the methanol extract of the aerial parts of Oenanthe javanica(Umbelliferae). Their chemical structures were identified as isorhamnetin(1), uracil(2), adenosine(3), persicarin(4) and uridine(5) by comparison of their spectral data with those of authentic samples and compounds 2, 3 and 5 were isolated from Oenanthe javanica for the first time. Compound 1 showed the strong radical scavenging activity among the compounds isolated from Oenanthe javanica.

• 생약학회지
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• 제28권2호
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• pp.88-92
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• 1997

The full NMR assignment of hispidulin 7-0-neohesperidoside (1) isolated from Cirsium japonicum var. ussuriense was made with the aid of 2D correlation NMR techniques such as HMQC and HMBC. To investigate detoxification of bromobenzene-induced hepatic lipid peroxidation by compound 1, hepatic lipid peroxide level and the activities of enzymes responsible for production and removal of epoxide were studied. The level of lipid peroxide elevated by bromobenzene was significantly reduced by compound 1. This compound administered daily over one week before intoxication with bromobenzene did not affect the activities of aminopyrine N-demethylase, aniline hydroxylase, glutathione S-transferase. Epoxide hydrolase activity was decreased significantly by bromobenzene, which was restored to the control level by pretreatment of persicarin. The results suggest that the bromobenzene-induced hepatic lipid peroxidation by compound 1 is reduced by enhancing the activity of epoxide hydrolase, an enzyme removing bromobenzene epoxide.