• 한국토양비료학회지
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• 제16권3호
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• pp.280-285
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• 1983

Subterraneum 클로바의 생장(生長)에 미치는 pH와 칼슘의 영향(影響) 및 품종간(品種間) 반회차이(反灰差異)를 수경재배(水耕栽培) 실험(實驗)으로 조사(調査)하였다. 1. 산성(酸性)(pH 4.5이하(以下))에서 발아율(發芽率)을 제외(除外)한 조사조(調査調)된 모든 생장요인(生長要因)이 저하(低下)되었다. 2. 산성(酸性)에 의한 생장장해(生長障害)는 높은 농도(濃度)의 칼슘(3.0mM)처리(處理)로 감소(減少)되었다. 3. 산성(酸性)에 대한 반응(反應)에는 품종간(品種間)에 차이(差異)가 있어 Mt. Barker는 조사(調査)된 세 품종중(品種中) 가장 높은 발아율(發芽率) 및 가장 긴 유근신장(幼根伸長)을 보였으나, 지상부(地上部) 및 근건물중(根乾物重), 근장(根長) 및 측근(側根)의 수(數)에 있어서는 Tallarook가 가장 높은 값을 나타내었다.

• 한국환경과학회지
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• 제24권12호
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• pp.1681-1689
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• 2015

This study was conducted to investigate days after anthesis (DAA) and fruit after-ripening period (FAP) for seed-harvesting of high quality watermelon seeds. Fruit weight and number of seed per fruit increased according to DAA, while those did not significant about FAP. Ratio of cotyledon at whole seed was higher about 2 to 4% compared to seed coat irrespective of DAA and FAP. Germinability of watermelon was not a significant effect by DAA, however, it had differences by FAP. Percent of germination (PB) was below 50%, when 30 days maturated fruits after anthesis was omitted ripening, while PB was increased to 92% by ripening. In addition, seeds at DAA 40 and FAP 20 were higher general seedling vigors (hypocotyl length, diameter etc.) in BP test. Results indicated that considering seed productivity, it had maximized seed viability at DAA 40 and FAP 20.

• 한국작물학회지
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• 제32권4호
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• pp.417-424
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• 1987

보리종자의 노화과정에서 일어나는 생리화학적인 활성을 비교 검토하여 종자세를 예측하기에 적합한 방법을 구명하기 위하여 새쌀보리, 부호보리 및 두산2002의 3품종을 ‘Wire-mesh tray’ 방법으로 41$^{\circ}C$, 상대습도 100%로 조절하여 1일에서 8일간 인위노화시켰다. 종자세의 지표로서 발아율$\times$유아의 길이로 표시한 값과 각종 매개면수와의 관계를 비교하였다. 발아시험에 의한 방법이 보리종자의 퇴화진전을 확실히 반영해 주고 있으며, ATP 검정과 GADA 검정방법을 제외한 저온발아시험, 전기전도검정 및 테트라조리움 검정방법은 보리 종자세평가에 적합한 방법임을 알 수 있었다. 특히 저온발아시험에 의한 유아장과 데트라조리움 검정법이 가장 효과적인 방법임을 알 수 있었다.

• Animal cells and systems
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• 제2권2호
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• pp.239-242
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• 1998

Arabidopsis trp1 mutant plants, deficient in phosphoribosyI anthranilate transferase (PAT) activity, accumulate anthranilate compounds, which render them blue fluorescence. The visible phenotype of trp1 makes the PAT gene an excellent reporter gene in the mutant. In order to develop a system for the homologous recombination using the phenotypic characteristic of trp1-100, we established optimum conditions for the isolation and regenera tion of protoplast from auxin-conditioned, trp1-100 root cultures. Trvptophan had to be supplemented in the germination medium for the efficient cell division and subsequent plant regeneration. When 10 uM tryptophan was added to the germination medium, we obtained the highest yield of protoplasts ($3{\times}10^6 cells/g$) and the best viability (92%). Thirty percent of root protoplast derived from meristematic cells underwent cell division within 5 days in callus-induction medium. Regenerated rosette leaves (2-3 mm) were transferred to rooting medium and finally acclimated to the soil for flowering.

• 한국환경복원기술학회지
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• 제7권3호
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• pp.86-95
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• 2004

This study was conducted to develop recycle revegetation methods for the restoration of the steep slopes by using fresh wood chip from construction sites. In general, the fresh wood chips can be used as soil media for the restoration works, because they can increase infiltration of rainfall and give enough porous to breathe and elongate for the root growth as well as economic value. The experiment was carried out to compare the effect of fresh wood chips from different mixing with soil, organic material and macromolecular compound which used for slope restoration works conducted by Hyunwoo green(Ltd.). The main results by monitoring for two years are summarized as follows; 1. The soil media made with low percentage of fresh wood chip covered quickly by herb plants. Especially, the soil mixture Type C (wood chip 20%) showed 80 percent ground coverage within two months after seeding. 2. The soil mixture type E (wood chip 40%) and type F (wood chip 50%) which contains more fresh wood chips than soil type C was under 30 percent ground coverage because wood plants are germinated well. If the restoration works aims at making forest, then the soil type E and F would be recommended than using soil type C. 3. Among the woody plants, Ailanthus alfissima, Pinus rigida, Pinus densiflora, and Albizzia julibrissin showed high percentage of germination rates and vigorous growth. In case of shrubs, Lespedeza cyrtobotria and Indigofera pseudo-tintoria scored high percentage of germination rates. 4. In native plants, Chrysanthemum indicum, Artemisia princeps, Lutos corniculatus and Imperata cylindrica showed high percentage of appearance. In case of introduced herbs, Coreopsis lanceolata, Coreopsis tinctoria and Oenothera oborata grew so vigorously. 5. The soil types which including fresh wood chips over 30-40 percentage showed the most diverse plant composition and the most effective germination rates and growth pattern with woody plants. 6. This works to develop recycle revegetation methods using fresh wood chips need more efforts for monitoring the exact effect of fresh wood chips as the soil media.

• 한국작물학회지
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• 제49권1호
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• pp.46-51
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• 2004

The film coated snap bean (Phaseolus vulgaris) seeds with five different coating materials treated with 3% increase in seed weight were planted at sandy loam soil controlled moisture content of 18, 19, 20 and 21 %. The oxygen diffusion rate (ODR) was calculated from the different moisture content soil. The number of normal seedlings, seedling vigor, and seedling capability in field (seed vigor x dry matter weight) were observed at 9 days after planting and compared to the changes of ODR. The germination rate and ODR were sharply decreased simultaneously in the seeds planted at 21 % soil moisture content. Seedling emergence did not occur at all as the soil moisture content increased above 22 %. Hence this value should be considered as the threshold of soil moisture content for seedling emergence. An ODR value under 20% did not influence the percent emergence significantly. The certain difference observing in the emergence at the same ODR was not related clearly to the condition of soil. So it can be assumed that the limit of soil moisture content for the emergence of snap bean was approximately 20%. The value of 18% soil moisture content may be considered as the optimum for snap bean emergence. There was close relationship between the mean value of ODR in different soil moisture contents and the emergence. The germination rates of the seeds coated with the different materials were quite different when the seeds were planted at 21 % soil moisture. Dry weight of the seedlings from film coated seeds was decreased slightly, but the germination rates were not much different from the non-treated control under relatively higher soil moisture content (21 %). Major factor lowering emergence rate was oxygen stress while film coating act as a minor constraint for snap bean sown in excess soil moisture condition.

• The Plant Pathology Journal
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• 제32권1호
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• pp.1-7
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• 2016

Responses of Botryosphaeria dothidea to interrupted wetness periods were investigated under in vivo and in vitro conditions. Conidia of B. dothidea were allowed to germinate on apple fruits under wetting condition at $25^{\circ}C$ for 5 hr. They were air-dried for 0, 1, 2 or 4 hr, and then rewetted at $25^{\circ}C$ for 5 hr. Following an initial wetness period of 5 hr, 83% of the conidia germinated. The percent conidial germination increased to 96% when wetting was extended continuously another 5 hr. However, no further conidial germination was observed when wetting was interrupted by dry periods of 1, 2 and 4 hr, resulting in 83, 81 and 82%, respectively. The mean length of the germ tubes was $37{\mu}m$ after 5 hr of wetting and elongated to $157{\mu}m$ after 10 hr of continuous wetting. On the other hand, interruption of wetting by a dry period of 1 hr or longer after the 5 hr of initial wetting arrested the germ tube elongation at approximately $42{\mu}m$ long. Prolonged rewetting up to 40 hr did not restore germ tube elongation on slide glasses under substrate treatments. Model simulation using weather data sets revealed that ending infection periods by a dry period of at least 1 hr decreased the daily infection periods, avoiding the overestimation of infection warning. This information can be incorporated into infection models for scheduling fungicide sprays to control apple white rot with fewer fungicide applications.

• 한국작물학회지
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• 제30권3호
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• pp.245-251
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• 1985

땅콩품종에 대한 입중의 변이를 조사하였으며, 동일품종에서의 종자크기에 따른 발아력, 생육 및 수량을 비교하는 한편 자엽을 인위적으로 절단, 조절했을 때의 생육과 수량에 대한 반응을 검토하였다. 1. 입중의 변이는 소립품종보다 대립종에서 크게 나타났으며, 2. Cold test에 의한 발아시험결과 종자가 작을수록 발아율이 낮았으며 포장출아율도 떨어졌다. 3. 종자크기에 따른 후기생육의 차이는 없었으나 소립품종의 경우는 종자가 큰 것을 파종했을 때 수량이 많았다. 4. 자엽의 일부를 절단하였을 때는 개화성기의 생육이 정상종자보다 저조했으나 협비대기이후에는 차이가 없었다. 5. 소립품종에서는 자엽절단이 수량을 감소시키나대립종에서는 정상립과 차이가 없었다.

• 한국초지조사료학회지
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• 제5권1호
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• pp.79-83
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• 1985

우리나라에서 많이 보급되고 있는 사배체품종(四倍體品種)들의 채종적기(採種適期)를 구명(究明)하기 위하여 Giant, Wase king, Wase hope, Tetrone를 공시(供試)하여 1984년 축산시험장(畜産試驗場) 시험포장(試驗圃場)에서 개화후(開花後) 10일(日)부터 34일(日)까지 주기적(週期的)으로 6회(回)에 걸쳐 종실(種實)의 수분함량(水分含量) 탈립(脫粒), 천립중(千粒重), 발아율(發芽率)을 조사(調査)하였다. 1. 수분함량(水分含量)은 개화후(開花後) 24일(日)까지는 서서히 감소(減少)하다가 24일(日)부터 급격히 감소(減少)하였으며 감소정도(減少程度)는 일당평균(日當平均) 1.5%이었다. 2. 천립중(千粒重)의 증가(增加)는 개화후(開花後) 24일(日)에 최대(最大)에 도달하였으며 Wase king이 가장 무거웠고 Giant가 제일 가벼웠다. 3. 탈립(脫粒)은 개화후(開花後) 17일(日)부터 20일(日) 사이에 시작(始作)되었으며 그 후에는 급격히 증가(增加)하였다. 4. 발아율(發芽率)은 개화후(開花後) 10일(日)에 약 50%이었고 24일(日)에는 90%에 도달하였으며 그 후에는 증가되지 않았다. 5. 채종적기(採種適期)는 개화후(開花後) $24{\sim}27$일(日)로서 수분함량(水分含量) $55{\sim}44%$, 천립중(千粒重) 3.6g, 발아율(發芽率) 약 90%, 탈립수(脫粒數) 10이삭당 $2{\sim}5$ 개(個)이었다.

• 한국작물학회지
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• 제31권4호
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• pp.447-453
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• 1986

콩 품종(williams)의 발아와 묘신장을 두 온도, 세 종자, 수분함양 및 Polyethylene glycol-800(PEG) 처리와 무처리에서 측정하였다. 분할구배치법 사반복으로 실시하였으며 시료를 2시간부터 8일까지 각처리 조합에서 채취하였다. 콩 종자를 0.2% Thiram을 첨가한 30% PEG 여액에 놓아 15$^{\circ}C$에서 6 일간 침청후 증류수로 선정하여 25.5$^{\circ}C$에서 3 일간 건조하여 10개의 처리 또는 무처리 종자를 plastic petri-dish안의 9 cm 여과지에 놓아 수분함양에 정당한 증류수를 첨가하였다. Parafilm으로 밀봉하여 항온무광조건의 처리 온도에서 발아시켰다. 수분함양이 50% 또는 이하인 처리에서는 두 온도 모두 묘신장이 일어나지 않았다. PEG를 이용한 삼투처리는 15$^{\circ}C$에서는 묘수분 흡수와 체신장간에 정의 상관 효과를 보였으나 3$0^{\circ}C$에서는 거의 없었다. 종자 또는 묘수분함량은 발아기간동안 초기에 급속히 증가하여 15$^{\circ}C$에서는 56시간에, 그리고 3$0^{\circ}C$에서는 28시간에 60%에 도달하였다. 묘신장은 종자수분함양이 15$^{\circ}C$에서는 60%에서 시작되었지만 3$0^{\circ}C$에서는 20시간에 시작되었다. 콩묘는 15$^{\circ}C$에 비하여 3$0^{\circ}C$에서 사배 이상 신장되었다. 발아기간 동안의 수분흡수양상은 이 시험에서는 두 단계로 특정되었다.