• Journal of Plant Biology
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• 제39권3호
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• pp.167-172
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• 1996

Efficient plant regeneration has been achieved via organogenesis in the red pepper plant (Capsicum annum L.). Shoots were induced from seed explants of cultivar 'Friendship' on Murashige and Skoog's (MS) basal medium supplemetned with; NAA or IAA, and BAP or zeatin. Seed explants on the medium supplemented with 0.1-0.3 mg/L IAA and 2-5 mg/L zeatin for 2 weeks vigorously formed normal shoots in more than 90% of the explants. When these were transferred to MS medium containing 0.5-1.0 mg/L GA, 90-100% of the shoots have elongated within 1-2 weeks. The elongated shoots rooted in media supplemented with 0.3 mg/L NAA. It was revealed that this method is very rapid and efficient regeneration system for red pepper and regenerated plants can be obtained after only 5-6 weeks of culture.

• 식물병연구
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• 제10권3호
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• pp.203-208
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• 2004

붉은 고추 탄저병균의 우점종인 Colletotrichum gloeosporioides는 C. acutatum에 비해 병원성이 강하였다. C. gloeosporioides는 G와 R계통이 존재하였으며, G계통이 R계통보다 더욱 강한 병원성을 보였다. 붉은 고추 품종간의 병원성 검정 결과, 금세기 품종이 가장 감수성이었고, 파페트 품종이 가장 약한 감수성이었다. 참깨, 홍화, 마, 그리고 딸기와 같은 다른 기주에서 분리되어진 C. gloeosporioides는 붉은 고추에 감염을 일으켰다.

• Food Science and Biotechnology
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• 제18권5호
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• pp.1076-1082
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• 2009

Six pesticides were determined in hot pepper leaves after successive processing steps of washing, blanching, and drying. The tested pesticides included dichlofluanid, flusilazole, folpet, iprodione, ${\lambda}$-cyhalothrin, and lufenuron. Each pesticide was singly applied to the leaves of the pepper plants, which were being cultivated in a greenhouse. The processing factors were dependent on the type of pesticide, and were in the following ranges: 0.09-0.73 by washing, <0.00-0.48 after blanching, and <0.00-3.30 after drying. Only lufenuron showed a processing factor of more than 1, at 3.30 in dried leaves, while the processing factors of the other pesticides were less than 1. The removal ratios of the tested pesticides by washing ranged from 27 to 90%. The blanching step increased their removals by 10-25%. However, drying did not have an effect on residue reduction. Finally, after proceeding to the drying step, removal ratios ranged from 85 to 100%, with the exception of lufenuron at 47%.

• The Plant Pathology Journal
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• 제32권1호
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• pp.65-69
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• 2016

In plant virus ecology, weeds are regarded as wild reservoirs of viruses and as potential sources for insect-mediated transmission of viruses. During field surveys in 2013-2014, three Leonurus sibiricus plants showing virus-like symptoms were collected from pepper fields in Daegu, Seosan, and Danyang in Korea. Molecular diagnosis assays showed that the collected L. sibiricus samples were infected with either Tomato spotted wilt virus (TSWV), Pepper mild mottle virus (PMMoV), or Beet western yellow virus (BWYV), respectively. Since this is the first identification of TSWV, PMMoV, and BWYV from L. sibiricus, complete genome sequences of three virus isolates were determined to examine their phylogenetic relationships with the previously reported strains and isolates. Phylogenetic analyses performed using full genome sequences of the viruses showed the isolates of TSWV and PMMoV obtained from L. sibiricus are closely related to the pepper isolates of the corresponding viruses. Our results suggest that L. sibiricus could act an alternative host and reservoir of viruses that cause damages in pepper fields.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.335-341
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• 2007

For the development of qualitative and quantitative PCR methods of genetically modified (GM) pepper developed in Korea, a capsanthin-capsorubin synthase (CCS) gene was used as the endogenous reference gene. The primer pair ccs-F/R amplifying the pepper endogenous gene gave rise to an amplicon of 102 bp. No amplified product was observed when DNA samples from 16 different plants were used as templates. The construct-specific primer pairs amplifying the junction region of the bar gene and Ti7 introduced in GM pepper gave rise to an amplicon of 182 bp. Quantitative PCR assay was performed using a TaqMan probe and a standard plasmid as a reference molecule, which contained both an endogenous and event-specific sequence. For the validation of this method, the test samples containing 0.1, 1, 3, 5, and 10% GM pepper were quantified.

• The Plant Pathology Journal
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• 제37권3호
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• pp.307-314
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• 2021

Pepper (Capsicum annuum L.) is an important agricultural crop worldwide. Recently, Colletotrichum scovillei, a member of the C. acutatum species complex, was reported to be the dominant pathogen causing pepper anthracnose disease in South Korea. In the present study, we isolated bacterial strains from rhizosphere soil in a pepper field in Gangwon Province, Korea, and assessed their antifungal ability against C. scovillei strain KC05. Among these strains, a strain named BS1 significantly inhibited mycelial growth, appressorium formation, and disease development of C. scovillei. By combined sequence analysis using 16S rRNA and partial gyrA sequences, strain BS1 was identified as Bacillus velezensis, a member of the B. subtilis species complex. BS1 produced hydrolytic enzymes (cellulase and protease) and iron-chelating siderophores. It also promoted chili pepper (cv. Nockwang) seedling growth compared with untreated plants. The study concluded that B. velezensis BS1 has good potential as a biocontrol agent of anthracnose disease in chili pepper caused by C. scovillei.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.68-68
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• 2022

The pepper mild mottle virus (PMMoV), belonging to the tobamovirus genus, is currently one of the most destructive pathogens in pepper production. Tobamoviruses have been classified in terms of increased pathogenicity as pathotypes P₀, P₁, P_1,2, P_1,2,3 and P_1,2,3,4, based on their ability to infect systemically Capsicum L⁰ , L¹ , L² , L³ and L⁴ resistant plants, respectively. Two hundred eighty pepper germplasms and 5 reference accessions known as resistant L alleles, were analyzed to select the resistance cultivars against PMMoV- P_1,2,3 (CV130614-2) using bioassay and genetic markers. The susceptible accessions showed systemic symptom when inoculated with PMMoV- P_1,2,3. However, accessions including IT223737, were resistant as they developed necrotic local lesions only on inoculated leaves, whereas no symptoms were observed on the upper leaves. Moreover, RT-PCR results for detecting the presence of virus were also negative. Thus, those accessions will be used as a novel source to facilitate introduction the resistant gene into commercial cultivars of pepper.

• 식물병연구
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• 제30권1호
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• pp.13-19
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• 2024

In July 2022 and 2023, black mold symptoms were observed sporadically on fruits of sweet pepper (Capsicum annuum) plants grown in a greenhouse located in Suwon, Korea. The incidence of black mold on the fruits was 5-24% (average 14.8%) in variety SP-504 (yellow and elongate type) and 1-8% (average 5%) in variety SP-505 (red and round type) investigated. Four single-conidium isolates of Alternaria sp. obtained from the diseased fruits were identified as Alternaria alternata based on the morphological characteristics and molecular phyogenetic analyses. The isolates were tested for pathogenicity to sweet pepper fruits of varieties SP-504 and SP-505 through artificial inoculation. The isolates mostly induced large lesions on fruits of the two varieties in the wound inoculation, but only two isolates small lesions on fruits of the variety SP-504 in the non-wound inoculation. No lesions formed on fruits of the variety SP-505 in the non-wound inoculation. The pathogenicity tests revealed that susceptibility of sweet pepper fruits to the disease differs between the varieties. The symptoms induced by pathogenicity tests with the isolates were similar to those observed on fruits from the greenhouse investigated. This is the first report of A. alternata causing black mold on sweet pepper fruits in Korea.

• 한국균학회지
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• 제43권4호
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• pp.253-259
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• 2015

고추 탄저병은 국내 고추재배에 가장 큰 피해를 일으키며, Colletotrichum acutatum이 주요 원인균이다. 본 연구에서는 포장에서 고추탄저병의 방제와 식물생장촉진효과를 선발된 길항미생물을 이용하여 평가하였다. 4개의 길항미생물은 이전 연구를 통하여 고추포장(GJ01, GJ11)과 갯벌(LB01, LB14)에서 선발하였다. 4개의 길항미생물은 대조균주 EXTN-1을 포함하여 C. acutatum과 대치배양에서 길항효과를 보였다. 식물생장촉진효과를 알아보기 위해 고추종자의 발아율과 초기생장효과, 그리고 포장에서 식물의 생장효과를 검정하였다. 그 결과 4개의 선발균주는 모두 식물생장효과가 있었다. 그 중에서도 GJ01은 초기생육에서 가장 높은 생장효과를 보였으며, GJ11은 포장에서 가장 높은 고추수확량을 얻었다. 그리고 포장에서 탄저병의 방제효과는 4개의 길항미생물 처리에 의해 63.2~72.5%의 방제가를 보였다. 현재 연구를 토대로 4개의 길항미생물은 고추 탄저병에 대한 잠재적인 생물학적 방제제로서의 가능성을 보여주었다.

• The Plant Pathology Journal
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• 제33권4호
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• pp.393-401
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• 2017

Efforts to control viral diseases in crop production include several types of physical or chemical treatments; antiviral extracts of a number of plants have also been examined to inhibit plant viral infection. However, treatments utilizing naturally selected microorganisms with activity against plant viruses are poorly documented. Here we report isolation of a soil inhabiting bacterium, Pseudomonas oleovorans strain KBPF-004 (developmental code KNF2016) which showed antiviral activity against mechanical transmission of tobamoviruses. Antiviral activity was also evaluated in seed transmission of two tobamoviruses, Pepper mild mottle virus (PMMoV) and Cucumber green mottle mosaic virus (CGMMV), by treatment of seed collected from infected pepper and watermelon, respectively. Pepper and watermelon seeds were treated with culture supernatant of P. oleovorans strain KBPF-004 or control strain ATCC 8062 before planting. Seeds germinated after treatment with water or ATCC 8062 yielded about 60% CGMMV or PMMoV positive plants, whereas < 20% of KBPF-004-treated seeds were virus-infected, a significantly reduced seed transmission rate. Furthermore, supernatant of P. oleovorans strain KBPF-004 remodeled aggregation of PMMoV 126 kDa protein and subcellular localization of movement protein in Nicotiana benthamiana, diminishing aggregation of the 126 kDa protein and essentially abolishing association of the movement protein with the microtubule network. In leaves agroinfiltrated with constructs expressing the coat protein (CP) of either PMMoV or CGMMV, less full-size CP was detected in the presence of supernatant of P. oleovorans strain KBPF-004. These changes may contribute to the antiviral effects of P. oleovorans strain KBPF-004.