• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.1038-1045
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• 2001

The electrokinetic technology was applied in bioremediation for the purpose of supplying a Pseudomonas strain capable of degrading diesel to contaminated soil bed, and their biodegradation of diesel was carried out after a desired cell distribution was obtained. Electrokinetic injection of the strain was made possible because the cells acted as negatively charged particles at neutral pH, and thus the cells were transported with a precise directionality through the soil mostly by the mechanism of electrophoresis and in part by electroosmosis. A severe pH change in the soil bed was formed due to the penetration of electrolysis products, which was harmful to the cell viability and cell transport. To achieve a desirable cell transport and distribution, the control of pH in soil bed by a recirculating buffer solution in electrode chambers was essential during the appliation of an electric field. The judicious selections of electrolyte concentration and conductivity were also important for achieving an efficient electrokinetic cell transport since a higher electrolyte concentration favored the maintenance of pH stability in soil bed, but lowered electrophoretic mobility on the other hand. With electrolyte solution of pH 7 phosphate buffer, a 0.05 M concentration showed a better cell transport buffer, a 0.05 M concentration showed a better cell transport than 0.02 M and 0.08 M. The cell under pH 8 were obtained, compared to the cells under pH 7 or pH 9 in a given time period Up to $60\%$ of diesel was degraded in 8 days by the Pseudomonas cell, which were distributed electrokinetically under the conditions of pH 8 ($1,800{\mu}S/cm$, a mixture of phosphate and ammonia buffers) and 40 mA in a soil bed of 15 cm length.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1827-1834
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• 2015

The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the binding site groove, on the function of SMG1 lipase. Site-directed mutagenesis was conducted at these sites, and each of the mutants was expressed in the yeast Pichia pastoris, purified, and characterized for their activity toward DAG and p-nitrophenol (pNP) ester. Compared with wild-type SMG1, F278A retained approximately 78% of its activity toward DAG, but only 11% activity toward pNP octanoate (pNP-C8). L103G increased its activity on pNP-C8 by approximately 2-fold, whereas F104G showed an approximate 40% decrease in pNP-C8 activity, and they both showed decreased activity on the DAG emulsion. The deletion of 103-104 retained approximately 30% of its activity toward the DAG emulsion, with an almost complete loss of pNP-C8 activity. The deletion of 103-104 showed a weaker penetration ability to a soybean phosphocholine monolayer than wild-type SMG1. Based on the modulation of the specificity and activity observed, a pNP-C8 binding model for the ester (pNP-C8, N102, and F278 form a flexible bridge) and a specific lipid-anchoring mechanism for DAG (L103 and F104 serve as "anchors" to the lipid interface) were proposed.

• Journal of Pharmaceutical Investigation
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• 제36권3호
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• pp.169-174
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• 2006

The aim of this work was to prepare tenoxicam-ethanolamine salt with improved physicochemical properties for transdermal application. Tenoxicam-ethanolamine salt was prepared in methylene chloride and its physicochemical properties were investigated by DSC and FT-lR. The broad peak of tenoxicam around 3600-3200 $cm^-1$ was shifted to lower wavenumber and more broadened. The characteristic endothermic melting peak of tenoxicam appeared at $223^{\circ}C$. The melting peak of tenoxicam-ethanolamine salt was shifted to $159^{\circ}C$. In contrast to relatively small difference in the partition coefficients of tenoxicam and the tenoxicam-ethanolamine salt, large difference in aqueous solubility was observed. $Crovol^{\circledR}$ PK4O (PEG-12 palm kernel glycerides) provided the highest skin flux for both compounds. The order of the enhancing effect of the various vehicles tested was similar for tenoxicam and tenoxicam-ethanolamine salt, which indicated that their enhancing mechanism for tenoxicam and tenoxicam-ethanolamine salt is similar. Tenoxicam-ethanolamine salt had a higher skin flux than tenoxicam by 1.2- to 31.7-fold, depending on the vehicles used. It is suggested that the vehicles with medium HLB value, 1 double bond, and lower ethylene oxide chain length have a better ability to modify the permeability of the stratum corneum and to promote the effective penetration of tenoxicam and tenoxicam-ethanolamine salt.

• 만화애니메이션 연구
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• 통권47호
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• pp.439-461
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• 2017

중국 고대사회에 있어 효 문화는 가부장제도 아래에서 도덕규범의 실천적 원리였다. 조아(曹娥)는 중국의 대표적인 효의 상징인물로, 원래 무녀(巫女)였으나 한대의 주류 사상인 유가철학의 전파와 함께 효녀의 형상으로 전환되었으며, 이후 지역문화의 발전과 함께 민간의 신앙과 접목하여 수신(水神)의 개념으로 재탄생하기도 하였다. 조아설화의 전파과정 중, 구전과 문학적 가공은 상호적인 작용을 통해 영향을 주고받으며, 미화되거나 신성화되고 체계화 되었는데, 조아도상은 이러한 인류가 추구하는 보편적이며 온전한 사유적 형상의 체현이다. 예컨대, 현대사회에 있어 문화적 정체성과 함께 그 이면의 근본적인 내적 함의의 인식이 중요하다. 그러므로 조아의 효를 주제로 한 사회기반의 문화콘텐츠의 창작과 이를 통해 효가 가진 문화적 핵심가치를 찾는 것은 의미 있는 일이다. 본문은 조아설화와 효사상 그리고 문화원형을 사례중심으로 서술하였으며, 조아의 민간신앙적 신성화 측면에서 문학으로부터 파생된 가공과 도상의 형성 사이의 관계를 이해하고, 이미지의 변화와 조형성을 통한 문화콘텐츠로서의 가능성을 살펴보았다.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2002년도 Proceedings of the International Welding/Joining Conference-Korea
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• pp.561-569
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• 2002

Laser has been called a "Quantum Machine" because of its mechanism of generation since the development on July 7,1960.by T.H.Maiman. We can now use this machine as a tool for manufacturing in industries. At present, 45kW CO2 laser, 10kW Nd:YAG laser, 6kW LD pumped YAG laser and 4kW direct diode laser facilities are available for welding a heavy steel plate of 40mm in thickness and for cutting metals at high speed of 140m/min. Laser Materials Processing is no longer a scientific curiosity but a modern tool in industries. Lasers in manufacturing sector are currently used in welding, cutting, drilling, cladding, marking, cleaning, micro-machining and forming. Recently, high power laser diode, 10kW LD pumped YAG laser, 700W fiber laser and excimer laser have been developed in the industrialized countries. As a result of large numbers of research and developments, the modem laser materials processing has been realized and used in all kinds of industries now. In the present paper, metallurgical studies on laser materials processing such as porosity formation, hot cracking and the joint performances of steels and aluminum alloys and dissimilar joint are discussed after the introduction of laser facilities and laser applications in industries such as automotive industry, electronics industry, and steel making industry. The wave towards the use of laser materials processing and its penetration into many industries has started in many countries now. Especially, development of high power/quality diode laser will be accelerate the introduction of this magnificent tool, because of the high efficiency of about 50%, long life time and compact.

• 한국환경과학회지
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• 제16권12호
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• pp.1401-1409
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• 2007

The formation mechanism of the snow cells of the Yellow Sea associated with snowfall over the southwestern part of Korea on 4 February, 2004 has been investigated using special upper-air sounding and radar data obtained for the KEOP(Korea Enhanced Observing Period) Intensive Observing Period(IOP). Results show that the types of snow cells for the selected period are classified into L(Longitudinal)-mode, Low-level convergence, and T(Transverse)-mode with their evolution from L-mode to T-mode. In particular, the existence of low-level warm and humid layer associated with temporally southwesterly inflow for about 4 hours provides a favorable condition in forming the T-mode snow cells. The vertical depth of the T-mode snow cells is deeper than that of L-mode ones due to the southeastward penetration of cold and dry air into relatively warm and humid air. In addition, it is found that wind shear vector between 1000 hPa and 600 hPa is one of the factors which control the orientation of snow cells in formation embedded into the snowbands for the both modes.

• Journal of Advanced Marine Engineering and Technology
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• 제30권3호
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• pp.351-358
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• 2006

The capability of high pressure injection with small fuel quantify at all engine operating conditions is one of the main feature in common rail fuel injection system, which is used in small and light-duty Diesel engine. The key parameter for the better atomized fuel sprays and multiple injections of this common rail fuel injection control, that can be freely selected irrespective of the engine speed and load is the mechanism controlling the needle energizing and movement in high pressure Diesel injector. In the electro-hydraulic injector, the injection nozzle is being opened and closed by movement of the injector's needle which is balanced by pressure between the nozzle seat and the needle control chamber. This study describes the macroscopic spray structure characteristics of the common rail Diesel injectors with different electric driving method i.e. the solenoid-driven and piezo-driven type. The macroscopic spray characteristics such as spray tip speed. spray tip penetration and spray cone angle were investigated by the high speed spray, which is measured by the back diffusion light illumination method with optical system for the high speed temporal photography in a constant volume chamber pressurized by nitrogen gas. As the results, the prototype piezo-driven injector system was designed and fabricated for the first time in domestic case and the effect of injector's needle response driven by different drive type was compared between the solenoid and piezo-driven injector It was found therefore. that the piezo-driven injector showed faster needle response and had better needle control capability by altering the electric input value than the solenoid-driven injector.

• 한국결정성장학회지
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• 제10권3호
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• pp.226-232
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• 2000

침지노즐(SEN)의 재료로 사용되는 지르코니아/흑연 재질에서 용강 및 슬래그에 의한 침식의 형태를 살펴본 결과, 침식의 첫번째 기구는 부분안정화 지르코니아 내 안정화제인 CaO가 용강 및 슬래그에 의해 용해되면서 입방정 안정화 지르코니아가 단사정상으로 상전이하여 일어났는데, 상전이 시 부피팽창에 의한 균열이 발생하고 파괴가 진행되었고, 이로 인하여 미세 $ZrO_2$입자의 용해 및 탈락이 촉진되었다. 침식의 두 번째 기구는 슬래그가 큰 c-$ZrO_2$입자의 입계를 따라 침투하여 작은 입자들로 분리시키고, 작은 입자들이 용강과 슬래그에 탈락되어 진행되었다. 마지막 기구로는 입방정 지르코니아와 흑연이 반응하여 다공질의 미세한 ZrC 상을 형성하고, 이들이 slag에 용해되면서 침식이 진행되었다.

• 한국세라믹학회지
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• 제45권7호
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• pp.387-394
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• 2008

The Physical property changes of calcined clay by carburization were investigated studied. The carburization mechanism is the penetration of carbon which occurred during incomplete fuel combustion into crevice of clay structure. The experiments for elasticity and freeze-thaw resistance were conducted, and the results can be summarized as follows: Dynamic modulus of elasticity and also freeze-thaw resistance of calcined clay by carburization treatment increased more than 92% after testing 300 cycle, which was more improved than 88% of calcined clay. Therefore, it can decrease the possibility of winter-sowing, which is one the weakness of calcined clay. It is on the basis of the fact that the porosity of calcined clay by carburization treatment is about 12%, which indicates smaller pore spaces comparing with the 14% of porosity of calcined clay and those values were calculated by apparent porosity show and also supported by SEM images. Infrared emissivity of calcined clay by carburization treatment and calcined clay were respectively 0.92 and 0.9l at $80^{\circ}C$. However, those values were 0.91 and 0.88 at $200^{\circ}C$, which means infrared emissivity of calcined clay by carburization treatment shows 3.6% higher than the calcined clay. Moreover, within the wavelength range from 3 to $7\;{\mu}m$, while the calcined clay had low infrared emissivity, the calcined clay by carburization treatment had increased infrared emissivity. It is inferred that it was affected by carbon element that has high infrared absorptivity within this wavelength range.

• 대한기계학회논문집A
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• 제36권12호
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• pp.1577-1583
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• 2012

횡방향 관통 효율 강화 탄체(PELE)는 기폭장치가 없는 새로운 개념의 발사체이다. PELE 는 배면이 닫혀있는 고밀도 피복과 저밀도 충전재로 구성되어있다. PELE 의 폭발 특성을 연구하기 위해 AUTODYN-3D code 를 이용하여 발사체와 표적체의 모델을 구축하였다. PELE 의 의해 알루미늄-2024 합금 표적체를 천공하는 과정을 시뮬레이션으로 구현하였으며 또한 다양한 내부 충전재에 의해 분산되는 표적체의 파편 특성도 연구하였다. PELE 파편의 유한요소해석은 AUTODYN-3D code 의 추계학적 파괴기준을 사용하여 구현되었다. 내부 충전재의 팽창으로 인해 파편은 속도를 얻으며 횡방향으로 분산된다. 따라서 손상영역의 범위가 증강한다. 관통 및 횡방향 분산 과정에서 생성되는 파편은 내부 충전재의 충격 압력에 따라 그 양과 형태가 다른 것으로 나타났다.