• 한국식품영양과학회지
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• 제44권10호
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• pp.1504-1509
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• 2015

생물전환에 의한 고려엉겅퀴 pectolinarin의 pectolinarigenin 전환 시 지표성분의 함량 및 표준화를 위한 분석법 검증을 실시하였다. 분석법 검증 결과 HPLC를 이용한 분석방법에서 표준용액의 retention time과 발효 고려엉겅퀴 추출물의 retention time이 일치하였으며 동일한 spectrum을 나타내는 것으로 특이성을 확인하였다. Pectolinarin 및 pectolinarigenin의 검량선은 상관계수 값이 모두 1로 나타나 높은 직선성을 보여주어 분석에 적합함을 알 수 있었다. Pectolinarin 및 pectolinarigenin의 검출한계는 각각 $4.25{\mu}g/mL$, $2.46{\mu}g/mL$였고, 정량한계는 $12.88{\mu}g/mL$, $7.46{\mu}g/mL$로 나타났다. Pectolinarin 및 pectolinarigenin의 함량을 확인한 결과 고려엉겅퀴 0시간, 5시간 발효물에서는 pectolinarin만 관찰되었으며, 19시간 발효물에서 생물전환에 의해 pectolinarin이 pectolinarigenin으로 전환되는 것을 확인할 수 있었다. 그 이후 28시간부터 72시간까지의 고려엉겅퀴 발효물에서는 pectolinarigenin만 관찰할 수 있었다. 정밀도 측정 결과 pectolinarin 및 pectolinarigenin은 일간 정밀도에서 각각 0.9%, 0.5%의 정밀도를 보여주었으며 일내 정밀도에서는 각각 0.5%, 0.2%로 높은 정밀성을 나타내었다. 또한 pectolinarin은 99.7~104.0%, pectolinarigenin은 99.7~102.4% 범위의 회수율을 보여주어 실험방법에 대한 정확성을 검증하였다. 본 연구 결과 고려엉겅퀴의 지표성분인 pectolinarin 및 pectolinarigenin의 HPLC를 이용한 동시분석방법이 적합한 분석방법임이 검증되었다.

• 약학회지
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• 제43권1호
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• pp.1-4
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• 1999

Four flavonoids were isolated from adenophora remotiflora var. hirticalyx. Their structures were identified as quercetin (1), kaempferol (2), luteolin (3), $pectolinarigenin-7-O-{\beta}-glucopyranoside(4)$ on the basis of spectral data.

• 한국식품영양학회지
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• 제30권2호
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• pp.388-394
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• 2017

본 연구에서는 생물전환 발효고려엉겅퀴 주정추출물을 온도별(4, 25 및 $50^{\circ}C$), pH별(4.0, 7.0, 및 10.0)로 21일간 저장하면서 7일 간격으로 pectolinarin과 pectolinarigenin 함량, 총 페놀 함량, DPPH 라디칼 소거능 및 FRAP를 측정하여 안정성을 평가하였다. 발효고려엉겅퀴 추출물인 pectolinarin과 pectolinarigenin 함량은 저장기간이 증가함에 따라 감소하는 경향을 보였으며, 온도에 의한 영향보다 pH에 의한 영향이 큰 것으로 나타났다. pH 10.0에서 pectolinarin의 함량 변화는 초기 $2.13{\pm}0.05mg/g$에서 $1.60{\pm}0.01mg/g$으로, pectolinarigenin 함량 변화는 $24.96{\pm}0.07mg/g$에서 $0.45{\pm}0.02mg/g$으로 크게 감소하였다. 총 페놀 함량도 저장기간이 따라 같이 감소하는 경향을 보였으며, $50^{\circ}C$와 pH 10.0에서 큰 감소치를 보였다. 항산화 안정성은 온도별 저장조건에서는 DPPH 라디칼 소거능은 큰 차이를 보이지 않았고, pH 10.0에서는 크게 감소하는 경향을 보였으나, FRAP은 온도별, pH별 저장조건에 따른 큰 변화를 보이지 않았다. 이상의 저장 온도별, pH별 결과를 종합해 볼 때, 생물전환 발효고려엉겅퀴 주정 추출물은 저온($4^{\circ}C$)에서 산성(pH 4.0)과 중성(pH 7.0) 상태에서 안정하였다.

• 생약학회지
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• 제30권1호
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• pp.87-91
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• 1999

Two carotenoids and four flavonoids were isolated from the whole plant of Artemisia sylvatica Max. Their structures were identified as ${\beta}-carotene$, lutein, luteolin, rutin, $pectolinarigenin-7-O-{\beta}-glucopyranoside$ and luteolin-7-O-neohesperidoside on the basis of spectroscopic means.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.487-489
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• 1994

A flavone glycoside was isolated from the leaves of Betula platyphylla var. latifolia and characterized as $4, 6-Dimethoxy-5-hydroxyflavone-7-O-{\beta}-D-glucoside(pectolinarigenin-7-O-{\beta}-D-glucopy-ranoside)$ by method of chemical and NMR spectral analysis. $^13C-^1H$ long range coupling was confirmative for determination of its substituted position. In connection with this study, 6-C-Glucosylnalingenin and 6-C-Glucosylaromadendrin were confirmed its structures using this technique.

• 생약학회지
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• 제33권3호통권130호
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• pp.173-176
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• 2002

In search for the plant-derived antioxidant compound, it was found that the EtOAc, BuOH extracts obtained from the leaves parts of Oryza sativa L. which exhibited a significant antioxidant activity from 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical cleavage. Activity-guided fractionation on the basis of the inhibitory activity upon the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical cleavage. and repeated column chromatography afforded several antioxidant compounds from Oryza sativa L. The structures and stereochemistry of these compounds were established on the basis of analysis of spectra and some chemical transformations as follows: 5,7-dihydroxy- 8-methoxyflavone, $acacetin-7-O-{\beta}-rutinoside$, $pectolinarigenin-7-O-{\beta}-rutinoside$. At antioxidant activity test for isolated three compounds, antioxidant activity was showed too.

• Archives of Pharmacal Research
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• 제17권4호
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• pp.273-277
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• 1994

Six flavonoids were isolated from the aerial parts of Cirsium flavonoids were identified as apigenin, luteolin, $pectolinarigenin-7-O-{\betha}-D-glucopyranoside$, linarin, pectolinarin and $hispidulin-7-O-{\alpha}-L-rhamonopyranosyl-(1{\rightarrow}2)-{\betha}-D-glucopyranoside$ on the basis of chemical and spctral evidence.

• 생약학회지
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• 제36권2호통권141호
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• pp.145-150
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• 2005

Nine compounds were isolated from the methanol extract of the aerial parts of Cirsium nipponicum. Their structures were identified to be phytene-1,2-diol (1), ${\beta}-sitosterol$ (2), pectolinarigenin (3), epilupeol acetate (4), 9,12-octadecadienoic acid (5), pinoresinol (6), apigenin (7), linaroside (8) and siparunoside (9) by spectroscopic means.

• 생약학회지
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• 제25권1호
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• pp.73-75
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• 1994

A flavone glycoside was isolated from the aerial parts of Cirsium nipponicum Makino in good yield and identified as pectolinarigenin $7-O-{\alpha}-{_L}-rhamnopyranosyl(1{\longrightarrow}6)-{\beta}-{_D}-glucopyranoside$, pectolinarin, on the basis of chemical and spectroscopic evidence.

• The Korean Journal of Physiology and Pharmacology
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• 제28권3호
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• pp.229-237
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• 2024

Cough is a common symptom of several respiratory diseases. However, frequent coughing from acute to chronic often causes great pain to patients. It may turn into cough variant asthma, which seriously affects people's quality of life. For cough treatment, it is dominated by over-the-counter antitussive drugs, such as asmeton, but most currently available antitussive drugs have serious side effects. Thus, there is a great need for the development of new drugs with potent cough suppressant. BALB/c mice were used to construct mice model with cough to investigate the pharmacological effects of pectolinarigenin (PEC). Hematoxylin-eosin and Masson staining were used to assess lung injury and airway remodeling, and ELISA was used to assess the level of inflammatory factor release. In addition, inflammatory cell counts were measured to assess airway inflammation. Airway hyperresponsiveness assay was used to assess respiratory resistance in mice. Finally, we used Western blotting to explore the potential mechanisms of PEC. We found that PEC could alleviate lung tissue injury and reduce the release of inflammatory factors, inhibit of cough frequency and airway wall collagen deposition in mice model with cough. Meanwhile, PEC inhibited the Ras/ERK/c-Fos pathway to exhibit antitussive effect. Therefore, PEC may be a potential drug for cough suppression.