• Title/Summary/Keyword: pectin

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The Effect of Dietary Fat Levels and Sources of Dietary Fiber on Serum and Liver Lipids of Rats (지방의 섭취량과 첨가된 섬유소의 종류가 흰쥐의 체내 지질 수준에 미치는 영향)

  • Chang, You-Kyung;Youn, Hong-Jae
    • Journal of Nutrition and Health
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    • v.17 no.4
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    • pp.253-261
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    • 1984
  • The effect of fiber sources on lipid metabolism was investigated in relation to the level of dietary fat. After rats were fed each diet for 4 weeks, all animals were sacrificed to collect the liver, and blood samples by heart puncture. Total lipid, total cholesterol, phospholipid and HDL- cholesterol were determined in serum. Total lipid and total cholesterol were also determined in liver. These results were compared with the histochemical appearances by the method of Oil red-O staining. Higher level of fat in the diet appeared to increase the total lipid levels in serum and more fat in the liver cells were shown from the high fat diet judging from the E.M. pictures. Phospholipid concentration in serum was greater in the rats of high fat groups than those of the control or non-fat groups. High fat level caused to decrease the HDL- cholesterol concentration in serum. Pectin decreased serum and liver lipids and the deposit of fat in the liver cells. HDL-cholesterol levels in serum was increased by pectin. Whereas the cellulose supplementation had no effect to reduce serum and liver total lipid levels, and cellulose did not decreased the deposit of fat in the liver cells. From these results, it is concluded that dietary fat levels led to changes in the lipid metabolism of rats and pectin may exhibit a lowering total lipid contents in serum and liver. Cellulose may slightly elevate serum and liver levels, or have no lowering effect.

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Manufacturing of Monodisperse Pectin Hydrogel Microfibers Using Partial Gelation in Microfluidic Devices (미세유체 장치에서 부분젤화법을 이용한 단분산성 펙틴 하이드로젤 미세섬유의 제조)

  • Jin, Si Hyung;Kim, Chaeyeon;Lee, Byungjin;Shim, Kyu-Rak;Kim, Dong Young;Lee, Chang-Soo
    • Clean Technology
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    • v.23 no.3
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    • pp.270-278
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    • 2017
  • This study introduces a method to easily fabricate highly monodisperse pectin hydrogel microfibers in a microfluidic device by using partial gelation. The hydrodynamic parameters between the pectin aqueous solution and the calcium ions containing oil solution are precisely controlled to form a stable elongation flow of the pectin aqueous solution, and partial gelation of the pectin aqueous solution is performed by the chelating of the calcium ions at the interface between the two phases. The partially gelled pectin aqueous solution is phase-separated from the oil solution in an aqueous calcium chloride solution outside the microfluidic device and is completely gelled to produce monodisperse pectin hydrogel microfibers. The thickness of the pectin hydrogel microfiber is controlled in a reproducible manner by controlling the volumetric flow rate of the initially injected pectin aqueous solution. The pectin hydrogel microfibers were 200 to 500 micrometers in diameter and had a coefficient of variation below 5% under all thickness conditions, indicating that the pectin hydrogel microfibers produced by partial gelation are highly monodisperse. In addition, biomaterials can be immobilized to the pectin hydrogel microfibers produced by a single process, demonstrating the possibility that our pectin hydrogel microfiber can be used as carriers for biomaterials or tissue engineering.

Changes in Pectin and Pectin Degrading Enzymes Activity during Storage of Kiyomi Tangor Produced in Jeju (제주산 만감류 청견의 저장 중 펙틴 및 펙틴분해효소 활성의 변화)

  • 강문장;임자훈;고정삼
    • Food Science and Preservation
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    • v.8 no.2
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    • pp.131-139
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    • 2001
  • Kiyomi tangor(Citus unshiu x sinensis) was stored at 3$\^{C}$ and 85% relative humidity, and the changes in firmness, pectin degrading enzymes activity and other physicochemical properties of citrus fruits during storage were investigated. Decay ratio and weight loss during 180 days’ storage were increased gradually to 13.0% and 12.9%, respectively. Firmness of fruits with 2 mm probe was decreased gradually from 808.7 g-force to 406.4 g-force, and moisture of peel and flesh were decreased from 76.5% to from 89.6% to 87.6% during storage, respectively. Exo-polygalacturonase activity of peel after 150 days’ storage were increased gradually to 558.09 units/100g. Pectin methylesterase activity of peel and flesh were increased from 14.7 units/g to 2.3 units/g, and from 9.4 units/ml to 2.7 units/ml at 150days’ storage, respectively. Endo-polygalacturonase activities were not changed notably during storage. Alcohol-insoluble solid(AIS) of peel was not changed notably. During storage of the fruits water soluble pectin(WSP) of peel and flesh were increased from 474.49 mg/100g to 614.29mg/100g, and from 66.91mg/100g to 92.74mg/100g as wet basis, respectively. Hexameta-phosphate soluble pectin(HMP) of peel were decreased from 405.5mg/100g to 270.43mg/100g, hydochloric acid soluble pectin(HSP) of peel was also decreased from 544.02mg/100g to 412.64mg/100g during storage. Total pectin substance(TPS) of peel and flesh were decreased from 1,424.01mg/100g to 1,297.36mg/100g, and from 165.51mg/100g to 171.54mg/100g, respectively. Composition ratio of pectin was in order of WSP > HSP > HMP.

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A Study on Pectic Substances of Korean Apple Varieties (한국산 사과의 품종별 펙틴물질들에 관한 연구)

  • 임화재;이혜수
    • Korean journal of food and cookery science
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    • v.2 no.1
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    • pp.65-70
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    • 1986
  • Total pectin content of Korean apple varieties at optimum harvest time was deter-mined by carbazole reaction method and pectin fraction content in each variety was also determined. Properties of pectin extracted ty 2a Sodium hexa metaphesphate were compared with commercial pectin. Among apple varieties, pectin contents varied in the range of 0.26-0.48% on fresh basis and 1.76-4. 0% on dry basis. Ball's was the highest and then high in order of Spur early blaze, Golden delicious, Fuji. , Jonathan. In each pectin fraction, soluble pectin fractions in Rall's was the lowest and in both Spur early blaze and Golden Delicious were highest but insoluble fractions in the latter were lowest. Thub the changes in poetic substances in Spur early blaze and Golden delicious seem to he occurred: during early ripening. In the analysis of properties of isolated pectin, ash, anhydrogalacturonic arid7(AUA) content and methoxyl content were different among apple varieties. Ash and methoxyl content in all isolated pectin were higher and AUA content lower than in commercial pectin. Degree of esterification (DE) in all apple varieties were above 70%.

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Influence of Sources and Levels of Dietary Fiber on Lipid Composition in Rats (식이중에 첨가된 섬유소의 종류와 수준이 흰쥐의 체내 지질함량에 미치는 영향)

  • 서정숙
    • Journal of Nutrition and Health
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    • v.21 no.3
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    • pp.164-172
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    • 1988
  • This study was performed to investigate the effects of sources and levels of dietary fiber on lipid metabolism in rats. Male weanling rats of Sprague-Dawley were fed ad libitum a diet containing perctin or cellulose at 5%, 10% and 20% for 4 weeks. Rats fed pectin gained significantly less weight than rats fed cellulose. Feed efficiency ratio was decreased at 10% and 20% group rats of pectin compared to control rats. Pectin was effective in lowering liver cholesterol, total lipid and triglyceride content at all levels. Whereas the cellulose supplementation had no effect to reduce serum and liver lipids. Cellulose led to an accumulation of serum and liver cholesterol. The least accumulation of lipid was in fed a cholesterol free diet. It is likely that lipid metabolism is quite different between pectin and cellulose feeding.

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Single Oral Toxicity of Jeju Citrus Rind Pectin in Spraque-Dawley Rats

  • Shim, Kyoo-Jung;Choung, Se-Young
    • Biomolecules & Therapeutics
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    • v.11 no.2
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    • pp.109-111
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    • 2003
  • The single oral toxicity of Jeju citrus rind pectin (Jeju pectin) was studied in Spraque-Dawley rats of both sexes. In this study, rats were administrated orally with dosages of 100, 250 and 500 mg/kg of Jeju pectin. We daily examined number of deaths, clinical signs, body weights and gross findings for 14 days after Jeju pectin administration. When we administered different doses of 100, 250 and 500 mg/kg. We found no rats died in both sex after administration. Some clinical signs (decrease locomotor activity, salivation, soft stool, prone position, lacrimation, crouching position, convulsion, ataxic gait, incontinence of mine) were also observed during the experimental period.

Compaction Simulator Study on Pectin Introducing Dwell Time

  • Kim, Hyun-Jo;Venkatesh, Gopi
    • Journal of Pharmaceutical Investigation
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    • v.35 no.4
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    • pp.243-247
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    • 2005
  • Although many scientists have used pectin, its feasibility in terms of tablet manufacturability with a high speed machine has never been evaluated. Therefore, compactibility of different pectin types for large scale tableting operation has been evaluated. The compactibility behavior of powder pectins was studied by a compaction simulator. It was found that pectin on its own does not produce tablets of acceptable quality even at a punch velocity as low as 20 rpm (e.g. low tensile strengths, capping and lamination irrespective of applied compression force). Thus, dwell time was introduced and more hard compact was produced as relaxation time in die increases. It was concluded that frequent structural failure observed in both pectin types was due to lack of plastic deformation, poor compactibility and high elastic recovery.

Physicochemical Properties of Pectic Substances from Citrus Fruits (감귤과 유자중의 펙틴질의 이화학적 성질)

  • Rhee, Hun-Joo;Rhee, Hei-Soo
    • Korean journal of food and cookery science
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    • v.6 no.1
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    • pp.9-12
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    • 1990
  • This study was carried out in order to investigate the physicochemical properties of isolated pectic substances from Citron, Satsuma mandarin, Marumero, and Natsudaidai. Among varieties, Citron had the highest value of isnoluble pectin and Marumero had the hightest ratio of soluble pection. The total pectin contents isolated by 2% Sodium hexameta-phosphate(SHMP) varied in the range of 1.85% ~ 2.63%. Anhydrogalacturonic acid(AUA) contents were 78.64%~89.14% and were lower than commercial pectin. Isolated pectin contained above 7% methoxyl content and above 50% esterification. The apparent molecular weight of commercial pectin and isolated pectin were 72,000 and 42,000~46,000 respectively. Esterification and ester methoxyl content were the lowest and apparent molecular weight was the highest in Satsuma mandarin. Citron's values were a little higher in esterification and ester methoxyl content and lower in apparent molecular weight than the others.

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Effect of Various Cooking Method on Pectin in Vegetables (여러 가지 조리방법에 따른 펙틴 함량의 변화)

  • Gye, Su-Gyeong
    • Journal of Applied Tourism Food and Beverage Management and Research
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    • v.17 no.2
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    • pp.27-38
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    • 2006
  • The contents and physicochemical properties of fiber in 15 kinds of vegetables being consumed commoly in Korea were investigated, and the effects of various cooking and Kimchi fermentation) on fiber were studied. Contents of total pechin in raw vegetables were 0.89-2.75g/100g, and most were 1-2g/100g, on dry weight basis. Considering each fraction of pectic substances, the content of HWSP of vegetables ranged in 0.33-0.98g/100g, HXSP, 0.29-0.81g/100g and HCLSP, 0.30-1.40g/100g. HCLSP of three pectic fractions showed the greatest variation according to the kinds of vegetables. Every vegetables showed similar contents of three pectic fraction. Cooking decreased total pectin content in all vegetables regardless of cooking methods and the greatest reduction was observed in boiling. Among three pectic fractions, hot water soluble pectin(HWSP) increased, and sodium hexamtaphosphate soluble pectin(HXSP) and HCL soluble pectin(HCLSP) decreased.

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The Content and Chemical and Physical Properties of the Pectin in Tangerine Peel (한국산 감귤류 폐과피 내의 펙틴함량과 펙틴의 특성에 관한 연구)

  • Moon, Soo-Jae;Sohn, Kyung-Hee;Yun, Seon;Lee, Myung-Hae;Lee, Myung-Hee
    • Korean Journal of Food Science and Technology
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    • v.14 no.1
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    • pp.63-66
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    • 1982
  • The pectin content in tangerine peel and the characteristics of extracted pectin and its gel were determined. The pectin contents of citrus peel and citrus albedo were 11.52% and 9.3% on a dry weight basis, respectively The equivalent weight, methoxyl content and acetyl content of the extracted citrus peel pectin were 596, 13.41% and 0.125% respectively. The intrinsic viscosity and molecular weight of extracted citrus peel pectin were higher than those of commercial citrus pectin samples. The anhydrouronic acid content and jelly grade of the extracted pectins were 98.4% and 138.1, respectively. These were higher than those of commercial citrus pectin. In the textural characteristics of gels, the gel made from the extracted pectin was shown to have lower values in hardness, cohesiveness, adhesiveness and gumminess than the gels made from commercial citrus pectins.

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