• Title/Summary/Keyword: pea

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Variation of Potato virus Y Isolated from Potato, Tobacco, Pea and Weeds in Korea on the C-terminal Region of Coat Protein Gene and 3'Non-translated Region

  • Yun, W.S.;Jung, H.W.;Oh, M.H.;Hahm, Y.I.;Kim, K.H.
    • The Plant Pathology Journal
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    • v.18 no.3
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    • pp.130-137
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    • 2002
  • Potato virus Y (PVY) is one of the most important viruses in many field crops in Korea. In this study, 31 PVY isolates were isolated from infected potato (Solanum tuberosum), tobacco (Nicotiana tabacum), pea (Pisum sativum), and weeds (Veronica persica, Lamium amplexicause and Capsella bursa-pastoris) showing different mosaic symptoms in Jeonbuk, Chungnam, Gangwon, and Gyeongbuk areas in Korea. The 640 nucleotide region containing the C-terminal portion of coat protein (CP) gene and 3'non-translated region (NTR) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using PVY-specific oligonucleotide primers. Sequence analyses of the amplified DNA fragments showed that the C-terminal portion of CP gene was not significantly different from that of previously reported PVY strains from potato (PVY-OK and -T) and tobacco (PVY-VN) in Korea. Homologies of the deduced CP amino acid sequences were 93.3-99.0% to corresponding regions of the other PVY strains including PV $Y^{N}$, PV $Y^{o}$ , PV $Y^{OK}$ , PV $Y^{T}$ , and PV $Y^{VN}$ . In contrast the sequences located at the 3'-NTR showed more diverse sequence homologies (76.4-99.7%). These results indicate that the C-terminal portion of the CP gene was relatively conserved while sequences at the 3'NTR were more diverse and variable over the host species and the regions where they were isolated.e isolated.

Synthesis and Herbicidal Activity of 1,3-Dimethyl-4-Acyl-Pyrazol-5-yl Carbamates (1,3-Dimethyl-4-Acyl-Pyrazol-5-yl Carbamates의 합성과 제초활성)

  • Lee, Chun-Soo;Park, Hyun-Soo;Kim, Jeung-Bae
    • Applied Biological Chemistry
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    • v.32 no.2
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    • pp.154-161
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    • 1989
  • 1,3-Dimethyl-4-acyl-5-hydroxypyrazoles were treated with methyl isocyanate to give corresponding carbamates. They were 1,3-dimethyl-4-benzoyl-pyrazol-5-yl N-methylcarbamate(V), 1, 3-dimethyl-4-(2-chlorobenzoyl)-pyrazol-5-y1 N-methyl-carbamate(VI), and 1, 3-dimethyl-4-(2,4-dichlorobenzoy1)-pyrazol-5-yl N-methyl-carbamate(VII). The structures of the compounds were fully identified by UV, IR, $^{1}H-NMR$ and $^{13}C-NMR$. The synthesized carbamate compounds were tested for the phytotoxicity on the seed germination and the seedling growth of rice, radish, green-pea and turf grass. At 50ppm concentraction, the germination of seeds was not significantly affected. At higher concentraction, however, phytotoxic tendency on the seed germination and seedling growth was observed. In addition, these compounds were less phytotoxic to rice and turf seedlings as compared with radish and green pea seedlings.

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BIOLOGICAL ACTIVITIES OF PRODUCTS FROM SUGAR CANE BAGASSE FERMENTATION BY Pleurotus sajor-caju (사탕수수 찌꺼기를 이용한 여름느타리 발효부산물의 생물활성)

  • Lee, Young-Keun;Chang, Hwa-Hyoung;Kim, Won-Rok;Kim, Jin-Kyu;Kim, Jae-Sung
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.39-42
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    • 1998
  • In order to evaluate the biological activities of some fractions from the fungal(Pleurotus sajorcaju) fermentation products of sugar cane bagasses, the antimutagenicity, the glyceollin elicitor activity on soybean and the effect on the stem elongation in pea were observed. The alcohol extract fraction and DMSO soluble fraction had excellent antimutagenicity even though it is weaker than that of the extracts from the fruiting bodies. All of the extracts had the ability to elicit glyceollins in soybean cotyledons and these extracts could be helpful for plants to protect themselves from pathogenic contaminations. IAA and the extracts had shown synergistic effects on pea stem elongation in all experimental groups positively determined.

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Interrelations Among Fast Food, Beverage Intake and Sociality, Anger Expression of Adolescents in the Busan Area (부산지역 일부 청소년의 패스트푸드, 음료 섭취와 사회성, 분노 표현과의 관계)

  • Lyu, Eun-Soon;Chae, In-Sook;Lee, Kyung-Hae
    • Korean Journal of Community Nutrition
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    • v.13 no.6
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    • pp.829-839
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    • 2008
  • The purpose of this study was to investigate the relation of the fast food and beverage intake on sociality and anger expression of adolescents. Questionnaires were distributed to the adolescents of 599 middle and high school students in Busan. According to the results, the preference-intake frequency analysis (PEA) on fast food grid, high preference and high intake frequency were 'dukbokki', 'chicken' and 'mandu' and low preference and high intake frequency were 'ramyon', 'gimbab. PEA on beverage grid, high preference and high intake frequency were 'milk-dairy product', 'fruit juice', 'isotonic beverage' and low preference and high intake frequency were 'carbonate drink'. The intake frequency of 'pizza', 'sandwich', 'udong', and 'dukbokki' had a positive relationship with sociality. 'Hamburger', 'chicken', 'french fry', 'gimbab', 'mandu', and 'ramyon' showed a positive relationship with anger-out. The intake frequency of 'carbonated drink' had a negative relationship with anger-control, but 'green tea' showed a positive relation with it. 'Carbonate drink', 'isotonic beverage', 'coffee', and 'milkshake' had a negative relationship with anger-out. The explanation power ($R^2$) of intake of fast food and beverage on sociality was $0.019{\sim}0.038$, and 'carbonated drink' and 'coffee' had a negative influence on sociality. The explanation power ($R^2$) of intake of fast food and beverage on anger expression was $0.011{\sim}0.041$, and 'carbonated drink' had a negative influence on angercontrol. 'Hamburger', 'carbonated drink', and 'coffee' showed a positive influence on anger-out. From these results, it was necessary to develop the practical eating-out habits program on proper fast food and beverage choice for adolescents.

Lipid and Carbohydrate Contents in the Adult Hemolymph during Flight of the Oriental Tobacco Budworm (Helicoverpa assulta (Guenee)) (비행중인 담배나방의 혈림프내 지질과 탄수화물의 함량변화)

  • 정진교;부경생
    • Korean journal of applied entomology
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    • v.31 no.4
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    • pp.329-337
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    • 1992
  • Studies were carried out to investigate changes of lipid and carbohydrate contents in the hemolymph of the Oriental tobacco budworm(Helicoverpa assulta (Guenee» adults during flight and hormonal effects on mobilization of energy sources in the hemolymph. During a few minutes after flight, both sexes showed a rapid increase in lipid content and the high level was maintained for about 2 hours. But carbohydrate content in the hemolymph during flight showed almost no change but a slight increase seen during the first 10 min of flight in males only. Synthetic adipokinetic(Lom-AKH- n), hypertrehalosemic(Bld-HrTH) hormones and brain/ corpora cardiaca extract of H. assulta adult elevated lipid and carbohydrate contents in hemolymph and the effect was much more pronounced for lipid. These results suggested that lipid is a main fuel for flight activity and lipid mobilization is under the hormonal control. And this study showed that both adipokinetic and hypertrehalosemic factors may exist in H. assulta and these factors may have similar structures to those of Mas-AKH, Hez-HrTH, Lom-AKH- n or Pea-HrTH.

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Physicochemical Properties of Starch from Cow Pea (동부 전분의 이화학적 특성)

  • Kim, Hyang-Sook;Kweon, Mee-Ra;Ahn, Seung-Yo
    • Korean Journal of Food Science and Technology
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    • v.19 no.1
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    • pp.18-22
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    • 1987
  • Starch granules of cow pea were oval with their sizes of $6-22{\mu}$m. X-ray diffraction pattern of starch granules showed some crystallinity at $2{\theta}$ $15^{\circ}$, $17.1^{\circ}$, $18.1^{\circ}$, $22.9^{\circ}$. The blue value of starch was 0.370, amylose contents 28.1% and water binding capacity 70.6%. Swelling power of the starch increased rapidly from $65^{\circ}C$ to $75^{\circ}C$ thereafter increased less rapidly. Solubility showed the same pattern as the swelling power. Optical transmittance of 0.2% starch suspension was increased rapidly from $70^{\circ}C$ and the gelatinization between $70^{\circ}C$ and $90^{\circ}C$ was single stage. Amylogram of 4% starch solution showed no peak viscosity but 6% starch solution showed peak viscosity.

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The Spatial Characteristics of Stratification in Deukryang Bay, Korea (밀도류 효과에 으히나 득령만의 성층변동 특성)

  • Byung-Gul Lee;Kyu-Dae Cho
    • Journal of Environmental Science International
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    • v.7 no.2
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    • pp.223-232
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    • 1998
  • The spatial characteristics of stratification In Deukryang Bay were studded using observed data and analytical models. From the description of the density structure and its the potential energy anomaly (PEA) from observed data along longitudinal direction (from the mouth to head of the bays, we found that the stratification Intensity could be changed strongly by density current effect during the spring-neap tidal cycle, and depth variation. To find out density current effect for the formation of the stratification In detail, we Implemented a diagnostic approach by using the modified analytical model including density current, tidal current, surface heating and wind stirring. The model allowed for the observed similarities for the whole domain in the bay and increased tidal mixing efficiency value s up to 0.006-0.007 as compared to the results without density current effect. We found that the density current effect was also an important key factor In determining the formation of the spatial distribution of stratification.

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Changes in the Activity of IAA Oxidase during Chilling Pea Seedlings (저온처리에 따른 완두 유모의 IAA Oxidasee 활성(活性)의 변화)

  • Park, Ro-Dong;Suh, Young-Tack;Shin, Yong-Kwang
    • Applied Biological Chemistry
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    • v.26 no.2
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    • pp.132-136
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    • 1983
  • The indole-3-acetic acid(IAA) oxidase activity of shoot and root tips of etiolated pea seedlings (Pisum sativum L. var. Sparkle) during and after chilling was determined. The IAA oxidase level of root tips was 4 to 15 times as high as that of shoot tips. During chilling the seedlings apical and subapical 5 mm shoot sections increased in IAA oxidase activity but apical and subapical 5 mm root sections decreased. When chilled plants were returned to $25^{\circ}C$ to recover, the enzyme activity had a tendency to restore to the activity level of controlled plants.

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A Study on the Relationship between the Amount of Toothpaste Used for Children by Daycare Teachers and the Methods of Squeezing Toothpaste (세치제 짜는 방법에 따른 보육교사의 어린이용 세치제 사용량 조사)

  • Son, Jung-Hui;Park, Il-Soon;Ma, Deuk-Sang
    • Journal of the Korean Society of School Health
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    • v.22 no.1
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    • pp.67-72
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    • 2009
  • Purpose: The purpose of this study was to examine the relationship between the toothpaste squeezing methods and the amount of toothpaste that the daycare teachers apply for the children in order to prevent the overuse of fluoride-containing toothpaste among children, and to understand their educational experiences on oral health and their awareness of the right amount of toothpaste used. Methods: The subjects in this study were 87 teachers at 16 different daycare centers in the city of Sokcho, Gangwon Province. They were interviewed to find out whether they had ever received oral health education and whether they were cognizant of the right amount of toothpaste to be used. And a transverse technique and a pea-sized squeezing technique were selected to look for connections between their toothpaste squeezing methods and the amount of toothpaste used by them. Results: 89.7 percent of the daycare teachers investigated taught to preschoolers how to do toothbrushing after they took meals and had a snack. 36.8 percent of the daycare teachers had children squeeze toothpaste on their own. The amount of toothpaste applied by the daycare teachers at a time was 0.58g. When they utilized two other squeezing methods, transverse technique and pea-sized squeezing technique, they used each 0.38 g (p<0.005) and 0.38 g (p=0.405) of toothpaste. Conclusion: To prevent children from being overly exposed to fluoride-containing toothpaste, daycare teachers should learn about the right amount of toothpaste to be used when oral health education is provided, and the transverse technique should be recommended to ensure the use of the proper amount of toothpaste.

Expression of Pea Superoxide Dismutase Gene in Transgenic Cucumber (Cucumis sativus L.) Plants (형질전환 오이(Cucumis sativus L.) 식물체에서 완두 Superoxide Dismutase 유전자의 발현)

  • 김재훈;오승용;이행순;조만현;이은모;우인식;곽상수
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.3
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    • pp.201-206
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    • 1998
  • To develop the fruits of cucumber (Cucumis sativus L.) producing high yields of superoxide dismutase (SOD), the MnSOD cDNA from pea (Pisum sativum) under the control of the cauliflower mosaic virus 35S promoter was introduced into cucumber using Agrobacterium tumefaciens (strain LBA 4404)-mediated transformation. The kanamycin-resistant shoots were selected on the selection medium containing MS basal salt, 1.0 mg/L zeatin, 0.1 mg/L IAA, 300 mg/L claforan, and 100 mg/L kanamycin. After 6 weeks of culture on the selection medium, the shoots were transferred to MS medium containing 0.2 mg/L NAA to induce roots. PCR analysis using the primers for neomycin phosphotransferase (NPTII) gene revealed that three plantlets were transformed. The fruits of one transgenic plant had approximately 3.2-fold higher SOD activity than those of non-transgenic plants. MnSOD isoenzyme band was strongly detected on native gel in fruits of transgenic plants.

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