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Effect of Short-term and Long-term Preservation on Motion Characteristics of Garole Ram Spermatozoa: A Prolific Microsheep Breed of India

  • Joshi, Anil;Bag, Sadhan;Naqvi, S.M.K.;Sharma, R.C.;Rawat, P.S.;Mittal, J.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.11
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    • pp.1527-1533
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    • 2001
  • Garole is a prolific, rare, less known and small size Indian sheep breed found in low and humid Sunderban region of West Bengal. Although information on stored Garole ram liquid semen upto 24 h is available, but there is a need to further investigate the short-term and long-term preservability of Garole ram semen for extensive utilization of this valuable germplasm by artificial insemination. The aim of the present study was to apply computer-assisted sperm analysis technique for assessing the motion characteristics of Garole ram semen stored (i) in liquid state at refrigeration temperature for short-term preservation upto 48 h and (ii) in frozen state at $-196^{\circ}C$ for long-term preservation after packaging in mini straws. Short-term preservation had a significant effect on motility (p<0.01) as the motility progressively decreased from 90.1% at 0 h to 85.5% and 73.2% after 24 and 48 h of storage, respectively. Although the decline in rapid moving sperms was also significant (p<0.01) on storage but the decrease was more pronounced at 48 h as compared to 24 h of storage period. Storage of chilled semen had also a significant effect on % linearity (p<0.05), % straightness (p<0.01), sperm velocities (p<0.01), amplitude of lateral head displacement (p<0.01) and beat frequency (pO.Ol) of spermatozoa. The replication had a significant effect for all the variables except average path and straight line velocity. However, the interactions of short-term storage and replication were non-significant for most of the variables except % of medium moving sperms, sperm velocities and beat frequency. On long-term preservation of Garole ram spermatozoa under controlled conditions the mean post-thaw recovery of 70.4 and 71.4% motile spermatozoa was achieved having 48.8 and 48.9% of rapidly motile spermatozoa, respectively in both the replicates. The effect of replication on cryopreservation was significant (p<0.05) on amplitude of lateral head displacement and beat frequency, but there was no significant effect on motility, rapidly motile spermatozoa, linearity, straightness and sperm velocities of frozen-thawed spermatozoa. It can be concluded from these results that an average 70% motility can be achieved on storage of Garole ram semen in chilled liquid state upto 48 h or in liquid nitrogen after freezing under controlled conditions in straws. However, further studies are required to evaluate the fertility of short-term and long-term preserved Garole ram semen for extensive use of this prolific sheep breed.

A Study on the Analysis Technique of Sequence Landscaping through the Application and Development of Visual Amount Calculation Program of Landscapes (경관의 시각량 산출 프로그램 개발과 적용을 통한 연속경관 시퀀스 분석기법 연구)

  • Koo, Min-Ah
    • Journal of the Korean Institute of Landscape Architecture
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    • v.44 no.5
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    • pp.12-25
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    • 2016
  • In this study, in order to facilitate analysis in a continuous sequence, including the concept of the landscape experience time, countless frames of a continuous landscape were extracted. The amount of visual elements in each frame was data-converted numerically to take advantage of the quantitative data necessary for landscape planning and design was calculated in the rhythm of the sequence. In Order to shoot video with the flow of the line of sight of experience in landscape districts and landscape control points along the landscape corridor which is a continuous path, each of the corresponding computer motion techniques. This study developed a CRVP Koo computer program to effectively calculate the continuous visual number of specific landscape components by extracting uncounted frames at regular intervals, and after verifying, attempting to apply this to the target site. Through the applied result, it was possible to extract the digitized quantitative rhythm for each component of each landscape, the margin of error is very small when compared with the results of manual in photoshop, it was able to overcome the drawbacks of the manual. Using the rhythm of the derived sequence, and those close to the experience of the landscape, it was possible to achieve quantitative analysis derived from a variety of perspectives as well as was possible to be used as quantitative basis data and analysis technique for landscape planning and design.

Delay Fault Test Pattern Generator Using Indirect Implication Algorithms in Scan Environment (스캔 환경에서 간접 유추 알고리즘을 이용한 경로 지연 고장 검사 입력 생성기)

  • Kim, Won-Gi;Kim, Myeong-Gyun;Gang, Seong-Ho
    • The Transactions of the Korea Information Processing Society
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    • v.6 no.6
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    • pp.1656-1666
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    • 1999
  • The more complex and large digital circuits become, the more important delay test becomes which guarantees that circuits operate in time. In this paper, the proposed algorithm is developed, which enable the fast indirect implication for efficient test pattern generation in sequential circuits of standard scan environment. Static learning algorithm enables application of a new implication value using contrapositive proposition. The static learning procedure found structurally, analyzes the gate structure in the preprocessing phase and store the information of learning occurrence so that it can be used in the test pattern generation procedure if it satisfies the implication condition. If there exists a signal line which include all paths from some particular primary inputs, it is a partitioning point. If paths passing that point have the same partial path from primary input to the signal or from the signal to primary output, they will need the same primary input values which separated by the partitioning point. In this paper test pattern generation can be more effective by using this partitioning technique. Finally, an efficient delay fault test pattern generator using indirect implication is developed and the effectiveness of these algorithms is demonstrated by experiments.

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The Effects of $PGF_2{\alpha}$ on Sexual Activity and Semen Collection Training (Prostaglandin $F2{\alpha}$ 투여가 수퇘지의 성행동과 정액 채취 훈련에 미치는 영향)

  • Hong, Joon-Ki;Ryu, Jae-Weon;Cho, Kyu-Ho;Kim, Myung-Jick;Park, Jun-Chul;Kim, In-Cheul;Jung, Il-Byung
    • Journal of Embryo Transfer
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    • v.24 no.1
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    • pp.29-32
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    • 2009
  • Prostaglandin $F_2{\alpha}$ ($PGF_2{\alpha}$) can facilitate release of epinephrine from the adrenal gland. The objective was to extend these findings and determine the effects of $PGF_2{\alpha}$ on sexual activity and semen collection training in sexually inexperienced boars. Boars (n=32; $281{\pm}18$ days of age) were moved individually once weekly to a semen collection room equipped with an artificial sow. Before entering the semen collection room, boar received i.m. treatments of $PGF_2{\alpha}$ at doses of 5 (n=8), 10 (n=8), or 20 (n=8), and control boar (n=8) were not treated. Reaction time (elapsed time after entering collection pen until the start of mounting) for boars receiving 5mg ($3.3{\pm}0.9\;min$), 10mg ($3.3{\pm}0.8\;min$) $PGF_2{\alpha}$ was shorter (p<0.05) than for controls ($6.7{\pm}0.9min$). Duration of ejaculation (min) per session was longer (p<0.05) for $PGF_2{\alpha}$ (10 mg, 20 mg)-treated boars ($7.3{\pm}0.7\;min$, $6.9{\pm}0.7\;min$), compared to control ($3.4{\pm}0.8\;min$). The number of training session per boars was less (p=0.056) for $PGF_2{\alpha}$ 10mg-treated boars ($1.0{\pm}0.4$), compared to control ($2.0{\pm}0.4$). Semen characteristic such as volume, concentration, the number of total ejaculated sperm, were similar for $PGF_2{\alpha}$-treated and controls. There was no apparent difference on sperm movement characteristics (Mot: motility, VCL : curve linear velocity, VSL : straight line velocity, VAP : average path velocity, LIN : linearity) after semen preservation by collected with or without $PGF_2{\alpha}$ treatment. In summary, administration of $PGF_2{\alpha}$ in boars increased the sexual activity and facilitated the training boars to mount an artificial sow for semen collection, but did not affect semen characteristic.

Compensation Characteristics of Distorted Channels in 200 Gbps WDM Systems using Mid-Span Spectral Inversion Method (200 Gbps WDM 시스템에서 Mid-Span Spectral Inversion 기법을 이용한 채널 왜곡의 보상 특성)

  • 이성렬
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.14 no.8
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    • pp.845-854
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    • 2003
  • In this paper, the characteristics of compensation for WDM channel signal distortion due to both chromatic dispersion and Ken effect in 1,000 km 200 Gbps(5${\times}$40 Gbps) WDM systems was investigated. The WDM system has a path-averaged intensity approximation(PAIA) mid-span spectral inversion(MSSI) as a compensation method. This system has a highly nonlinear dispersion shifted fiber(HNL-DSF) optical phase conjugator(OPC) in the mid-way of transmission line. In order to evaluate the degree of compensation, 1 dB eye opening penalty(EOP), bit error rate(BER) characteristics and power penalty of 10$\^$-9/ BER are used. It is confirmed that HNL-DSF is an useful nonlinear medium in OPC fur wideband WDM system with PAIA MSSI and that the optimal compensation for WDM channel distortion is achieved by the selection of pump light power of OPC, which equalize the conjugated light power into the second half fiber section with the input WDM signal light power depending on total transmission length, dispersion coefficient of fiber, OPC pump light wavelength, conversion efficiency of WDM channel in OPC.

Performance Analysis of Interference Cancellation Algorithms for an FM Based PCL System (FM 신호 기반 PCL 시스템에서 간섭 신호 제거 알고리즘의 성능 분석)

  • Park, Geun-Ho;Kim, Dong-Gyu;Kim, Ho Jae;Park, Jin-Oh;Lee, Won-Jin;Ko, Jae Heon;Kim, Hyoung-Nam
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.42 no.4
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    • pp.819-830
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    • 2017
  • An FM radio based PCL system is a passive radar technique for detecting the multiple moving targets from FM radio signals and tracking the trajectories of the targets by calculating the cross-correlation function of direct-path signal and target echo signals. However, the interference signals are received from a surveillance channel, which is designed to receive the target echo signals. Because of this problem, the target echo signals are masked by the strong interference signals and this makes it difficult to detect the true targets from the cross-correlation function. Adaptive filters are known as effective methods for suppressing the interference signals but there is a problem to present their accurate performances in the PCL system because many literatures used the cross-correlation function and the ratio of input and output power as a measure of the performance analysis. In this paper, a performance analysis method is proposed to evaluate the performance of interference cancellation algorithms. By using the property that each component of the filter weight vector is adjusted to suppress the specific interference signal, a performance measure of the interference signal suppression is defined by a function of adaptive filter weights. Based on the proposed method, we compare the performance of the adaptive filters used in the PCL system. Simulation results show that the proposed method can be very effective for evaluating the performance of interference cancellation algorithms.

Effect Analysis of Classical Line TI-21 type Audio Frequency Track Circuit from KTX Sancheon Return Current Harmonics (KTX산천 귀선전류고조파가 일반선 TI-21형 AF궤도회로에 미치는 영향분석)

  • Choi, Jae Sik;Kim, Hie Sik;Park, Ju Hun;Kim, Bun Gon
    • Journal of the Korean Society for Railway
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    • v.19 no.1
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    • pp.38-45
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    • 2016
  • The power transformation system of High Speed rolling stocks like KTX-Sancheon has shown excellent control capacities in the areas of riding comfortability, switching efficiency, safety and energy consumption due to technical developments in power-electronics, high speed & large scale integrated semiconductors and microprocessors. However, harmonics from IGBT, a high speed switching device used in the Convertor & Invertor equipment of rolling stocks have given rise to various problems in transformer substations, signaling systems, data transmission systems and facility monitoring systems. Especially, TI21 non-insulated track circuits have malfunctioned due to the influence of returning current harmonics which were generated at around of integer times of the number of power transformation equipment in the frequency domain. This paper, measures and analyzes various schemes to analyze the traveling path of the returning current harmonics generated due to the relationship between the rolling stocks and track circuits on site. Ultimately, theseschemes will be used to design high speed rolling stocks, AF track circuits and a common grounding network.

An Efficient Walkthrough from Two Images using Spidery Mesh Interface and View Morphing (Spidery 매쉬 인터페이스와 뷰 모핑을 이용한 두 이미지로부터의 효율적인 3차원 애니메이션)

  • Cho, Hang-Shin;Kim, Chang-Hun
    • Journal of KIISE:Computing Practices and Letters
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    • v.7 no.2
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    • pp.132-140
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    • 2001
  • This paper proposes an efficient walktlu-ough animation from two images of the same scene. To make animation easily and fast, Tour Into the Picture(TIP) enables walkthrough animation from single image but lacks the reality of its foreground object when the viewpoint moves from side to side, and view morphing uses only 2D transition between two images but restricts its camera path on the line between two views. By combining advantages of these two image-based techniques, this paper suggests a new virtual navigation technique which enable natural scene transformation when the viewpoint changes in the side-to-side direction as well as in the depth direction. In our method, view morphing is employed only in foreground objects , and background scene which is perceived carelessly is mapped into cube-like 3D model as in TIP, so as to save laborious 3D reconstruction costs and improve visual realism simultaneously. To do this, we newly define a camera transformation between two images from the relationship of the spidery mesh transformation and its corresponding 3D view change. The result animation shows that our method creates a realistic 3D virtual navigation using a simple interface.

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Wind and Bathymetry Effects on the Fresh Water Plume Structures (담수 확산에 미치는 바람과 해저 지형의 영향)

  • Lee, Jungwoo;Yun, Sang-Leen;Oh, Hye-Ceol
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.10
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    • pp.698-703
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    • 2014
  • The structures of fresh water plume depending on estuarine geometries and wind directions (upwelling, onshore, downwelling, and offshore winds) were studied using the Regional Ocean Modeling System (ROMS). Four idealized estuaries, which are different in bathymetry, were considered. The results showed that the fresh water plume was restricted close to the shore line where a river was connected to the sloping shelf rather than the flat shelf due to the fast momentum exchanges from x, y to z momentums on the sloping shelf. Mild upwelling and offshore winds (${\mid}{\tau}_{\omega}{\mid}=0.01Pa$) enhanced stratification on the contrast to previous studies which showed that winds destroyed stratification by enhanced vertical mixing. However, downwelling and onshore winds had similar effects on the vertical structure of the fresh water plume as in previous studies enhancing vertical mixing. The plume was confined above the underneath submarine channel, thus the plume path was directly affected by the direction of the submarine channel on the shelf.

Fast algorithm for incorporating start and goal points into the map represented in a generalized visibility graph (출발점과 목표점을 일반화 가시성그래프로 표현된 맵에 포함하기 위한 빠른 알고리즘)

  • Yu, Kyeon-Ah;Jeon, Hyun-Joo
    • Journal of the Korea Society for Simulation
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    • v.15 no.2
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    • pp.31-39
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    • 2006
  • The visibility graph is a well-known method for efficient path-finding with the minimum search space modelling the game world. The generalized visibility graph is constructed on the expanded obstacle boundaries to eliminate the "wall-hugging" problem which is a major disadvantage of using the visibility graph. The paths generated by the generalized visibility graph are guaranteed to be near optimal and natural-looking. In this paper we propose the method to apply the generalized visibility graph efficiently for game characters who moves among static obstacles between varying start and goal points. Even though the space is minimal once the generalized visibility graph is constructed, the construction itself is time-consuming in checking the intersection between every two links connecting nodes. The idea is that we build the map for static obstacles first and then incorporate start and goal nodes quickly. The incorporation of start and goal nodes is the part that must be executed repeatedly. Therefore we propose to use the rotational plane-sweep algorithm in the computational geometry for incorporating start and goal nodes efficiently. The simulation result shows that the execution time has been improved by 39%-68% according to running times in the game environment with multiple static obstacles.

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