• Journal of Korean Academy of Nursing
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• v.42 no.4
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• pp.517-524
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• 2012

Purpose: To compare and confirm the impact of prepregnancy body mass index on pregnancy outcome in women with a singleton conceived by assisted reproductive technology and spontaneously conceived pregnancy. Methods: A sample of 165 and 247 pregnant women with and without assisted reproductive technology were retrospectively recruited from electronic medical charts of C hospital. Results: There were significant differences between the two groups for maternal age, paternal age, length of marriage, prepregnancy body mass index, parity, spontaneous abortion experience, and preterm delivery. A prepregnancy body mass index of ${\geq}25$ was associated with higher risk for maternal and neonatal complication in the assisted reproductive technology group. Conclusion: The results indicate that a higher prepregnancy body mass index is associated with increased risks for adverse pregnancy outcomes for women using assisted reproductive technology. So these women need appropriate care to compensate for the risk.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.84-93
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• 2016

This study was conducted to determine family factors related to obesity of high school students. A descriptive correlational study was conducted with 384 high school students in I metropolitan city. Data were collected from February to April of 2015 using self-report structured questionnaires. Questionnaires included weight, height, individual characteristics of subjects and their familial characteristics. Collected data were analyzed by multiple logistic regression. Logistic regression analysis revealed that male gender (aOR = 4.37; 95% CI [1.26, 15.16]), having a non-regular paternal job (aOR = 3.10; 95% CI [1.15, 8.40]), and not being in a two parent family (aOR = 3.52; 95% CI [1.29, 9.61]) were statistically significant contributors to obesity. These results indicate that it is necessary to improve social awareness regarding the obesity risk of male adolescents. Health care providers should recognize the importance of family factors such as parental socioeconomic status and family structures.

• Korean Journal of Animal Reproduction
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• v.26 no.4
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• pp.361-368
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• 2002

The onset of pronucleus formation and DNA synthesis in porcine oocytes following the injection of porcine or murine sperm was determined in order to obtain insights into species-specific paternal factors that contribute to fertilization. After 44h in vitro maturation, spermatozoa was injected into the cytoplasm of oocytes. After injection, all oocytes were transferred to NCSU23 medium and cultured at 39'E under 5% CO2 in air. Similar frequencies of oocytes with female pronuclei were observed after injection with porcine sperm or with murine sperm. In contrast, male pronuclei formed 8 to 9 h following the injection of porcine sperm, and 6 to 8 h following the injection of murine sperm. After pronucleus formation maternally derived microtubules were assembled and appeared to move both male and female pronuclei to the oocyte center. A few porcine oocytes entered metaphase 22 h after the injection of murine sperm, but normal cell division was not observed. The mean time of onset of S-phase in male pronuclei was 9.7 h following porcine sperm injection and 7.4 h following mouse sperm injection. These results suggested that DNA synthesis was delayed in both pronuclei until the sperm chromatin fully decondensed, and the sperm nuclear decondensing activity and microtubule nucleation abilities of the male centrosome are cell cycle dependent.

• Journal of Families and Better Life
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• v.32 no.5
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• pp.41-61
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• 2014

The purpose of this study was to investigate whether differentiation of self mediates the relationship between parenting styles (i.e., care and overprotection) and the level of career decision-making among young adults. We separately measured the mother's and the father's parenting styles and five dimensions of differentiation of self that S. Je (1989) suggested. The data came from 387 young adults who were juniors and seniors at universities in Seoul and its surrounding cities. Using Baron and Kenny's (1986) steps based on regression, we found that the relationships between both caring and overprotective parenting styles and young adults' level of career decision-making were mediated by four dimensions of differentiation of self (i.e., intellectual functioning vs. emotional functioning, family projection process, emotional cutoff, and family regression) except for integration of self. The results were consistent for both the mother and the father. Bootstrapping tests showed that all of the mediation effects were statistically significant. This study contributes to the literature by showing that young adults' level of career decision-making is not simply determined by inputs such as parenting styles but is also mediated by differentiation of self. The results of this study are meaningful in that both the mother's and the father's parenting styles could make a difference in young adults' level of career decision-making through differentiation of self.

• Korean Journal of Veterinary Research
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• v.30 no.2
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• pp.123-127
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• 1990

By nuclear transplantation technology twenty eight mice have been produced after transfer of heterozygous biparental eggs. Also heterozygous gynogenetic eggs with two female pronuclei and heterozygous androgenetic eggs with two male pronuclei have been obtained by injecting a male or female pronucleus with Sendai virus into the perivitelline space of enucleated haploid zygotes at pronuclear stage. The success rate of enucleation, karyoplast injection and fusion of both the pronuclei was 80.3, 83.4 and 81.8%, respectively. The overall pronuclei fusion rates by this technique were 56, 50 and 56% in biparental, gynogenetic and androgenetic eggs, respectively. The evidence was ascertained that the gynogenetic and androgenetic eggs were also able to develop in vitro up to blastocyst stage, even though their developmental potential was greatly diminished beyond 2-cell stage. The gynogenetic eggs were able to develop in vivo up to day 10 of pregnancy, while the androgenetic eggs failed to develop in vivo during the same period.

• The Journal of the Korea Contents Association
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• v.16 no.12
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• pp.233-243
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• 2016

The movie offers a chance to observe the social signifier and the sensitivity of audiences who feel sympathy with the tragic history of crown prince Sado. While watching the movie, the audience halts between ideas whether to go back to "primary ego", or to play the unconscious hypocrisy or the ingrained social behavior which is to resolve the internal conflicts while they face two contrary opinions; one is the obedient subject to obey fatherness, as a symbol of historic value and convention in a society, and the other is the resistant body to break the bond of convention. The audience may feel some ambivalent feelings of the moral values between the paternal authority and the existential philosophical value of 'self'. This study will observe the audience's response of Sado toward the moral norms of the society when they face the signifier of father in the movie with experience the conflict between their resistant ego and madness. Also the study will discuss that the social landscape such as norms, order, and legal system is ever-changing and open to be subverted by the future even though audience hope that the present moral value with which we are content ever-last.

• Journal of Aquaculture
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• v.10 no.1
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• pp.39-47
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• 1997

Induction of hybrid and allotriploid between female rainbow trout, oncorhynchus mykiss and male cherry salmon, O. masou were performed. Triploid rate in allotriploid was 92% and the survival rates of allotriploid exceeded that of their hybrid counterparts. Karyological studies revealed that the hybrid had exactly the same intermediate diploid karyotypers as that of two parental species, while allotriploid had two sets of the maternal haploid complement and one set of the paternal haploid complement. Hybrid revealed an intermediate size in celluar and nuclear size of erythrocyte, while allotriploid revealed larger cellular and nuclear size of erythrocytes than hybrid. The number of nucleolar organizer regions of hybrid were 1 or 2, while those of allotriploids were 1, 2 or 3. These results proved that allotriploidization can lead to increase cytogenetical characters compared to counterpart hybridization.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.37-43
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• 2012

In this study, we invesgated a cytogenetic analysis of the Pacific triploid abalone, Haliotis discus hannai induced by low temperature treatment. We got a lot of mitotic metaphase chromosome spreads from the triploid and diploid Pacific abalones' hatched larvae (trochophores). The chromosome number of diploid abalone was 2n = 36 and that of triploid abalone was 3n = 54, so the chromosome number of triploid abalone was 1.5 times higher than that of diploid abalone. We developed a modified flow cytometric method for Pacific abalone from the existing methods. We uesd 51 months aged triploid and diploid Pacific abalones' hemolymph to get the DNA contents by flow cytometry. The DNA content of diploid abalone was 1.743 pg/cell and the DNA content of triploid abalone was 1.49 times higher than that of diploid one. It proved that triploid abalone was consisted with two sets of maternal diploid and one set of paternal genome.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.63-71
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• 2012

To assess parameters of mating system in seed orchard, such as outcrossing rates, number of potential pollen contributors, and degree of pollen contamination, seeds, produced in '77 plot of the Japanese red pine (Pinus densiflora S et Z) seed orchard at Anmyeon island, were collected in 2007 and analysed by nSSR and cpSSR markers. Estimates of outcrossing rates ranged from 91.2 to 100% (mean 97.7%) on the basis of the analysis of cpSSR haplotypes and from 81.6 to 100% (mean 95.3%) on the basis of the analysis of nSSR genotypes. By cross checking of both DNA markers, seeds, presumed to be products of self pollination on the basis of single marker, were confirmed as outcrossed seeds, which resulted in cumulative outcrossing rates of 98.9%. On the basis of pooled cpSSR haplotype of each seed, the number of pollen contributors and paternal contribution rates were estimated as 14.8 and 0.512, respectively. In conclusion, considering pretty high level of outcrossing rates observed in a seed orchard, good genetic potential of the seeds, produced in '77 plot of the seed orchard of Japanese red pines at Anmyeon island, may be guaranteed. Investigated results from the analysis of mating system of Japanese red pines in a '77 plot of the seed orchard may also be expected to provide useful information for the management and establishment of the seed orchard of the progressive generation.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.12
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• pp.1816-1825
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• 2019

Objective: We tried to analyze allele-specific expression in the pig neocortex using bioinformatic analysis of high-throughput sequencing results from the parental genomes and offspring transcriptomes from reciprocal crosses between Korean Native and Landrace pigs. Methods: We carried out sequencing of parental genomes and offspring transcriptomes using next generation sequencing. We subsequently carried out genome scale identification of single nucleotide polymorphisms (SNPs) in two different ways using either individual genome mapping or joint genome mapping of the same breed parents that were used for the reciprocal crosses. Using parent-specific SNPs, allele-specifically expressed genes were analyzed. Results: Because of the low genome coverage (${\sim}4{\times}$) of the sequencing results, most SNPs were non-informative for parental lineage determination of the expressed alleles in the offspring and were thus excluded from our analysis. Consequently, 436 SNPs covering 336 genes were applicable to measure the imbalanced expression of paternal alleles in the offspring. By calculating the read ratios of parental alleles in the offspring, we identified seven genes showing allele-biased expression (p<0.05) including three previously reported and four newly identified genes in this study. Conclusion: The newly identified allele-specifically expressing genes in the neocortex of pigs should contribute to improving our knowledge on genomic imprinting in pigs. To our knowledge, this is the first study of allelic imbalance using high throughput analysis of both parental genomes and offspring transcriptomes of the reciprocal cross in outbred animals. Our study also showed the effect of the number of informative animals on the genome level investigation of allele-specific expression using RNA-seq analysis in livestock species.