Korean Journal of Animal Reproduction (한국가축번식학회지)

The Korean Society of Animal Reproduction (한국동물번식학회)
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Onset of Pronuclear Formation and DNA Synthesis in Porcine Oocytes following Intracytoplasmic Injection of Porcine or Murine Spematozoa

  • Kim, N. H. (Department of Animal Science, Chungbuk National University Research Center for Transgenic Cloned pig) ;
  • Cui, X. S (Department of Animal Science, Chungbuk National University Research Center for Transgenic Cloned pig) ;
  • Kim, B. K . (Department of Animal Science, Chungbuk National University Research Center for Transgenic Cloned pig) ;
  • S. H. Jun (Department of Animal Science, Chungbuk National University Research Center for Transgenic Cloned pig) ;
  • D. I. Jin (Research Center for Transgenic Cloned pig Division of Applied Biological Sciences, Sunmoon University) ;
  • Lee, S. H. (Research Center for Transgenic Cloned pig Department of Nursing, Kongju National University) ;
  • Park, C. S. (Research Center for Transgenic Cloned pig Department of Animal science, Chungnam)
  • Published : 2002.12.01
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Abstract

The onset of pronucleus formation and DNA synthesis in porcine oocytes following the injection of porcine or murine sperm was determined in order to obtain insights into species-specific paternal factors that contribute to fertilization. After 44h in vitro maturation, spermatozoa was injected into the cytoplasm of oocytes. After injection, all oocytes were transferred to NCSU23 medium and cultured at 39'E under 5% CO2 in air. Similar frequencies of oocytes with female pronuclei were observed after injection with porcine sperm or with murine sperm. In contrast, male pronuclei formed 8 to 9 h following the injection of porcine sperm, and 6 to 8 h following the injection of murine sperm. After pronucleus formation maternally derived microtubules were assembled and appeared to move both male and female pronuclei to the oocyte center. A few porcine oocytes entered metaphase 22 h after the injection of murine sperm, but normal cell division was not observed. The mean time of onset of S-phase in male pronuclei was 9.7 h following porcine sperm injection and 7.4 h following mouse sperm injection. These results suggested that DNA synthesis was delayed in both pronuclei until the sperm chromatin fully decondensed, and the sperm nuclear decondensing activity and microtubule nucleation abilities of the male centrosome are cell cycle dependent.

Keywords

References

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