• Korean Journal of Child Studies
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• v.32 no.5
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• pp.13-28
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• 2011

This study explored the relationship between parental reaction to children's negative emotions, child's self-regulation and stress-coping behaviors. This study also examined the mediating effect of a child's self-regulation between parental reactions to children's negative emotions and a child's stress-coping behaviors. The sample included 407 elementary school 5th-6th grades and their parents (comprising 407 couples) in Seoul. The research results are summarized as follows. First, parental reactions to children's negative emotions were significantly related to the child's self-regulation and stress-coping behaviors. Secondly, it was also found that parental reactions to children's negative emotion were both partially and indirectly related to a child's stress-coping behaviors through the child's use of self-regulation.

• The Journal of the Convergence on Culture Technology
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• v.9 no.5
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• pp.769-778
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• 2023

This study compared and analyzed parental leave and child allowance, two major policies among solutions to the current serious low fertility rate problem, using unstructured data, and sought future directions and implications for related response policies based on this. The collection keywords were "low fertility + parental leave" and "low fertility + child allowance", and data analysis was conducted in the following order: text frequency analysis, centrality analysis, network visualization, and CONCOR analysis. As a result of the analysis, first, parental leave was found to be a realistic and practical policy in response to low fertility rates, as data analysis showed more diverse and systematic discussions than child allowance. Second, in terms of child allowance, data analysis showed that there was a high level of information and interest in the cash grant benefit system, including child allowance, but there were no other unique features or active discussions. As a future improvement plan, both policies need to utilize the existing system. First, parental leave requires improvement in the working environment and blind spots in order to expand the system, and second, child allowance requires a change in the form of payment that deviates from the uniform and biased system. should be sought, and it was proposed to expand the target age.

• Journal of Korean Public Health Nursing
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• v.23 no.2
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• pp.207-218
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• 2009

Purpose: In an attempt to develop a parental health improvement education program for day care children, survey questions were devised to measured the extent of parental health knowledge, health-guidance execution and needs. Method: Participants were 727 parents whose children attended daycare centers in J province informed consent was provided prior to participation. Result: Respondents were overwhelmingly (91.6%) mothers. Questions with high percentage of incorrect answers were "What should I do if my child swallows harmful drugs, bleach or detergents?" (98.1%) and "What should I do if insects or other bugs enter my child's ear?" (96.6%). In most cases, parental health-guidance execution exceeded 90% except in response to the survey question "Does your child have a dental check-up every 6 months" (51%). Taken together, parental education is necessary concerning growth and development (12.8%), nutrition (12.1%), healthy life styles (10.3%) and general health issues (10.0%). Conclusions: Parental health knowledge about preschooler's health varies with degree of income, education and type of employment. Consideration of these variables is important in a parental health education program.

• Journal of the Korean Home Economics Association
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• v.50 no.7
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• pp.59-66
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• 2012

The purpose of this study was to identify a causal relationship in the career preparation behavior, parental social support, career decision making self-efficacy and the career maturity of the pre-service elementary school teachers. A total of 374 questionnaires were used for data analysis, excluding the 23 copies deemed insincere in response. To ensure the reliability and validity of the questions, technical statistics of the frequency, ratio, average, standard deviation, skewness, and kurtosis via PASW 18.0, item-total correlation, the totality, and the reliability analysis. The structural analysis via AMOS 7.0 in the bootstrapping method was undertaken to perform the path analysis among the variables and to assess the suitability of the model. The findings of the study led to the following conclusions: First, the causal model for the career preparation behavior, parental social support, career decision making self-efficacy, and the career maturity of the pre-service elementary school teachers is suitable to empirical analysis on research variables. Second, the career decision making self-efficacy of pre-service elementary teachers has direct effect on career preparation behavior positively. Third, parental social support of the pre-service elementary teachers has indirect effects on the career preparation behavior positively.

• Journal of Families and Better Life
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• v.32 no.2
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• pp.53-75
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• 2014

The purpose of this study was to develop and validate the "Authentic Parental Competence Scale" for mothers with school-age children. Based on a literature review, the study found that 'authentic parental competence' could be divided into parenting comperence, self-system competence, and social competence. In addition, the scale comprised three factors consisting of 43. The study collected data through behavior event interview(BEI), focus group interview(FGI), and three times Delphi investigation. The scale for mothers of school-age children consisted of a total of 129 items, which included three different dimensions (knowledge, skill, and attitude), and four factors were extracted from factor analysis following a survey of 362 mothers. The factors were labeled as "ability of developmental positive parenting," "ability of playing a role as a citizen and making social contributions," "ability of self-understanding and interpersonal relations," and "ability of self-growth and self-determination." In addition, the results revealed that the "Authentic Parental Competence Scale" showed an appropriate level of construct validity, reliability of the scales, distribution of item response, and item discrimination.

• Journal of Microbiology and Biotechnology
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• v.32 no.1
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• pp.6-14
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• 2022

Brucella spp. are facultative intracellular pathogens that invade, survive and proliferate in numerous phagocytic and non-phagocytic cell types, thereby leading to human and animal brucellosis. Outer membrane proteins (Omps) are major immunogenic and protective antigens that are implicated in Brucella virulence. A strain deleted of the omp16 gene has not been obtained which suggests that the Omp16 protein is vital for Brucella survival. Nevertheless, we previously constructed an omp16 conditional deletion strain of Brucella, ∆Omp16. Here, the virulence and immune response elicted by this strain were assessed in a mouse model of infection. Splenomegaly was significantly reduced at two weeks post-infection in ∆Omp16-infected mice compared to infection with the parental strain. The bacterial load in the spleen also was significantly decreased at this post-infection time point in ∆Omp16-infected mice. Histopathological changes in the spleen were observed via hematoxylin-eosin staining and microscopic examination which showed that infection with the ∆Omp16 strain alleviated spleen histopathological alterations compared to mice infected with the parental strain. Moreover, the levels of humoral and cellular immunity were similar in both ∆Omp16-infected mice and parental strain-infected mice. The results overall show that the virulence of ∆Omp16 is attenuated markedly, but that the immune responses mediated by the deletion and parental strains in mice are indistinguishable. The data provide important insights that illuminate the pathogenic strategies adopted by Brucella.

• Phonetics and Speech Sciences
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• v.1 no.2
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• pp.51-59
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• 2009

This study was designed to investigate the effects of neonatal hearing screening program (NHSP) information on parental satisfaction with the Parent Satisfaction Questionnaire with Neonatal Hearing Screening Program (PSQ-NHSP) by Mazlan et al. (2006). The PSQ-NHSP consisted of four aspects including: information, personnel in charge of the hearing test, appointment activity, and overall satisfaction in the neonatal hearing screening program. A total of 106 parents (50 in the experimental group and 56 in the control group) participated in this study in one general hospital and two delivery clinics. The fifty parents in the experimental group received information and counseling with educational materials before filling out the PSQ-NHSP, but the fifty-six parents in the control group did not receive any counseling or education materials before completing the PSQ-NHSP. The PSQ-NHSP demonstrated excellent internal consistency reliability (${\sigma}=0.914$). The results of the study were as follows. First, the overall satisfaction ($3.77{\pm}0.81$) and personnel in charge of hearing test ($3.52{\pm}0.79$) aspects showed higher rates of satisfaction than the appointment activity aspect ($3.51{\pm}0.80$) for total subjects. Second, the overall parental satisfaction rate of the experimental group ($4.15{\pm}0.50$) was significantly higher than that of the control group ($3.09{\pm}0.53$) in all items. Lastly, thirty-two participants (30%) made at least one comment in response to the open-set items. A total of 29 comments were related to satisfaction with participating in the NHSP and II comments were related to dissatisfaction. In conclusion, to improve parental satisfaction it is important to provide parents with education and information about the NHSP before the test. In addition, PSQ-NHSP was found to be a useful instrument for identifying the benefits and shortfalls of the NHSP.

• Genomics & Informatics
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• v.11 no.4
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• pp.245-253
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• 2013

A radioresistant cell line was established by fractionated ionizing radiation (IR) and assessed by a clonogenic assay, flow cytometry, and Western blot analysis, as well as zymography and a wound healing assay. Microarray was performed to profile global expression and to search for differentially expressed genes (DEGs) in response to IR. H460R cells demonstrated increased cell scattering and acidic vesicular organelles compared with parental cells. Concomitantly, H460R cells showed characteristics of increased migration and matrix metalloproteinase activity. In addition, H460R cells were resistant to IR, exhibiting reduced expression levels of ionizing responsive proteins (p-p53 and ${\gamma}$-H2AX); apoptosis-related molecules, such as cleaved poly(ADP ribose) polymerase; and endoplasmic reticulum stress-related molecules, such as glucose-regulated protein (GRP78) and C/EBP-homologous protein compared with parental cells, whereas the expression of anti-apoptotic X-linked inhibitor of apoptosis protein was increased. Among DEGs, syntrophin beta 2 (SNTB2) significantly increased in H460R cells in response to IR. Knockdown of SNTB2 by siRNA was more sensitive than the control after IR exposure in H460, H460R, and H1299 cells. Our study suggests that H460R cells have differential properties, including cell morphology, potential for metastasis, and resistance to IR, compared with parental cells. In addition, SNTB2 may play an important role in radioresistance. H460R cells could be helpful in in vitro systems for elucidating the molecular mechanisms of and discovering drugs to overcome radioresistance in lung cancer therapy.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.712-720
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• 2007

To investigate clonal variations of recombinant Chinese hamster ovary(rCHO) clones in response to culture pH and temperature, serum-free suspension cultures of two antibody-producing CHO clones(clones A and B), which were isolated from the same parental clone by the limiting dilution method, were performed in a bioreactor at pH values in the range of 6.8-7.6, and two different temperatures, $33^{\circ}C\;and\;37^{\circ}C$. In regard to cell growth, clone A and clone B displayed similar responses to temperature, although their degree of response differed. In contrast, clones A and B displayed different responses to temperature in regard to antibody production. In the case of clone A, no significant increase in maximum antibody concentration was achieved by lowering the culture temperature. The maximum antibody concentration obtained at $33^{\circ}C$(pH 7.4) and $37^{\circ}C$(pH 7.0) were $82.0{\pm}2.6$ and $73.2{\pm}4.1{\mu}g/ml$, respectively. On the other hand, in the case of clone B, an approximately 2.5-fold increase in maximum antibody concentration was achieved by lowering the culture temperature. The enhanced maximum antibody concentration of clone B at $33^{\circ}C$($132.6{\pm}14.9{\mu}g/ml$ at pH 7.2) was due to not only enhanced specific antibody productivity but also to prolonged culture longevity. At $33^{\circ}C$, the culture longevity of clone A also improved, but not as much as that of clone B. Taken together, CHO clones derived from the same parental clone displayed quite different responses to culture temperature and pH with regards antibody production, suggesting that environmental parameters such as temperature and pH should be optimized for each CHO clone.

• BMB Reports
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• v.56 no.12
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• pp.669-674
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• 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection leads to various clinical symptoms including anemia. Lipocalin-2 has various biological functions, including defense against bacterial infections through iron sequestration, and it serves as a biomarker for kidney injury. In a human protein array, we observed increased lipocalin-2 expression due to parental SARS-CoV-2 infection in the Calu-3 human lung cancer cell line. The secretion of lipocalin-2 was also elevated in response to parental SARS-CoV-2 infection, and the SARS-CoV-2 Alpha, Beta, and Delta variants similarly induced this phenomenon. In a Calu-3 implanted mouse xenograft model, parental SARSCoV-2 and Delta variant induced lipocalin-2 expression and secretion. Additionally, the iron concentration increased in the Calu-3 tumor tissues and decreased in the serum due to infection. In conclusion, SARS-CoV-2 infection induces the production and secretion of lipocalin-2, potentially resulting in a decrease in iron concentration in serum. Because the concentration of iron ions in the blood is associated with anemia, this phenomenon could contribute to developing anemia in COVID-19 patients.