• Journal of Microbiology and Biotechnology
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• v.6 no.6
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• pp.386-390
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• 1996

The ${\beta}$-galactosidase gene from Lactococcus lactis subsp. lactis ATCC 7962 was cloned and its enzymatic properties were characterized, with a view to assessing its potential use as a selection marker in the food-grade cloning vector. Chromosomal DNA from L. lactis subsp. lactis 7962 was cleaved with PstI and ligated into pBR322 for transformation into Escherichia coli TGl. Transformants showing ${\beta}$-galactosidase activity possessed the pBR322 plasmid containing a 10 kilobase (kb) PstI fragment and this plasmid was named pCKL11. The cloned ${\beta}$-galactosidase gene came from the chromosomal DNA of L. lactis subsp. lactis 7962 was confirmed by Southern hybridization. A restriction map of pCKL11 was constructed from the cleavage of both pCKL11 and the purified 10kb insert fraqment. The. optimum pH of the ${\beta}$-galactosidase determined with the E. coli harboring the pCKL11 was 7.0. The optimum temperature was $50^{\circ}C$, while the pI of the enzyme was 7.4. These values were the same as those of the enzyme from the parent strain.

• Smart Structures and Systems
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• v.26 no.6
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• pp.721-733
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• 2020

The present study investigates the employing of piezoelectric patches in active control of a sandwich plate. Indeed, the active control and optimal patch distribution on this structure are presented together. A sandwich plate with honeycomb core and composite reinforced by carbon nanotubes in facesheet layers is considered so that the optimum position of actuator/sensor patches pair is guaranteed to suppress the vibration of sandwich structures. The sandwich panel consists of a search space which is a square of 200 × 200 mm with a numerous number of candidates for the optimum position. Also, different dimension of square and rectangular plates to obtain the optimal placement of piezoelectric actuator/senor patches pair is considered. Based on genetic algorithm and LQR, the optimum position of patches and fitness function is determined, respectively. The present study reveals that the efficiency and performance of LQR control is affected by the optimal placement of the actuator/sensor patches pair to a large extent. It is also shown that an intelligent selection of the parent, repeated genes filtering, and 80% crossover and 20% mutation would increase the convergence of the algorithm. It is noted that a fitness function is achieved by collection actuator/sensor patches pair cost functions in the same position (controllability). It is worth mentioning that the study of the optimal location of actuator/sensor patches pair is carried out for different boundary conditions of a sandwich plate such as simply supported and clamped boundary conditions.

• The Bulletin of The Korean Astronomical Society
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• v.45 no.1
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• pp.62.1-62.1
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• 2020

We present a scaling relation between black hole (BH) mass and bulge luminosity for 35 nearby (z<0.1) type 1 active galaxies, selected from the 70-month Swift-BAT X-ray source catalog. Thanks to the unbiased selection and proximity of the parent sample, our sample is suitable to study the physical connection between central black holes and host galaxies. We use the F814W images obtained with the Advanced Camera for Surveys on Hubble Space Telescope, to perform the imaging decomposition with GALFIT. With a careful treatment on the PSF model, we measure the I-band bulge brightness robustly. In combination with the BH mass estimated from a single-epoch spectroscopic data, we present the correlation between BH mass and bulge luminosity of the target AGNs. We demonstrate that our sample marginally lies off from the M(BH)-L(bulge) relation of inactive galaxies. We discuss possible physical origins of this discrepancy. Finally, we present how the relation depends on the photometric properties of AGNs and host galaxies, which may provide an useful insight on the co-evolution between BHs and host galaxies.

• Journal of Forest and Environmental Science
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• v.11 no.1
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• pp.81-101
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• 1995

Random amplified polymorphic DNA (RAPD) technology, a recent approach in molecular genetics, is much usable to select the elite trees and to maximize the genetic gain in forest tree breeding program, providing a clue to determine the genetic marker-trait correlation. This review intorduces research on bark thickness and breeding strategy in Pinus elliottii, Pinus caribaea and their hybrid by Queensland Forest Service and ForBio Research Pty Ltd, University of Queensland, which employ RAPD technology. Genetic linkage map of $F_1$ hybrids includes 186 RAPD markers and 16 linkage groups (1641 cM long in total) and 6 quantitative trait loci are located putatively for bark thickness. Following recent research results and experiences in pine breeding programs, the forseeable stages in the application and development are proposed for marker assisted selectin; stage 1-determination of species specific markers for genes controlling traits of commercial interest, and stage 2-determination of marker-allele association for specific allelic variants within pure species. As pines inherit their megagametophytes from the seed parent and zygotic embryos from both male and female parents, the determination of marker-trait correlation is possible even in embryo stage, eventually making ways for the early selection of elite individuals.

• Korean Journal of Social Welfare
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• v.58 no.1
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• pp.87-113
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• 2006

The research was performed responding to the growing needs of Korean foster care programs. It deals specifically with the people who voluntarily provide foster care despite the lack of Government support. The research has also made an effort to understand how the foster parents perceive their parent roles for the foster children. According to the research results based on the parents from the H foster care center, it showed that foster parents generally come from higher educational backgrounds compared to their equivalent age group and also similar or even higher income levels than the average family residing in an urban area. Conclusively, their traits in socio-demographic factors is said to be homogeneous. The research also focused on examining the role perceptions of Korean foster parents. The foster parents were well aware and educated on the significance of the children's psychological adjustments. However, the research recognized some role responsibilities that the parents failed to understand. Hopefully the foster care centers will take on such responsibilities and contribute in helping the parents realize their insufficiencies. Based on the results of the research, the researcher was able to recognize the important issues involved in the recruitment, selection, and education process for the foster parents. Additionally, new role adjustments are necessary among both foster parents and foster centers.

• Microbiology and Biotechnology Letters
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• v.30 no.1
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• pp.68-72
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• 2002

To obtain a yeast mutant with high RNA content and high growth rate, Saccharomyces cerevisiae MTY62 was mutated with ethylmethane sulfonate. Among the selected mutants that were sensitive to the high concentration of KCl, M40-10 strain was finally selected due to its rapid cell growth and high RNA content in the tube and baffled-flask cultures. In the batch culture of M40-10 mutant, the maximum specific growth rate ($\mu_{max}$) of $0.38 h^{-1}$ , RNA concentration of 3210 mg-RNA/1, and RNA content of 183 mg-RNA/g-DCW were obtained, which were 23%, 15%, and 12% increased levels, respectively, compared to those of MTY62 parent strain. The intermittent fed-batch culture of M40-10 strain resulted in the maximum cell concentration of 35.6 g-DCW/1, RNA concentration of 5677 mg/1, and RNA content of 160 mg-RNA/g-DCW. Through the constant fed-batch culture, the maximum cell concentration of 46.4 g-DCW/1, RNA concentration of 6270 mg-RNA/1, and RNA content of 135 mg-RNA/g-DCW were obtained. At the 20 h culture time in the fed-batch cultures of M40-10 strain, the cell and RNA concentrations were increased by 30% and 10%, respectively, over the parent strain MTY62. In addition, it was also found that the accumulated RNA within the mutant cell was not degraded until the end of fed-batch cultivation, indicating that the M40-10 cell is a mutant with weak acidic RNase activity.y.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.517-521
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• 2013

A new cultivar Dendranthema grandiflourm 'Dream Round' was developed at Gyeonggi-do Agricultural Research & Extension Services (GARES), Korea in 2010. The variety 'Dream Round' was initially derived from a cross in 2006 between 'Hebo' a seed parent, a spray chrysanthemum cultivar with white anemone type, and 'Samos' as a pollen parent, a spray chrysanthemum variety with white anemone type. The cultivar has anemone type with white petals. Trial evaluation was conducted from 2008 to 2010 for the selection of that cultivar, including a shading culture in spring and a retarding culture in summer. The flowering time of 'Dream Round' was October 24th, and year-round flowering was possible by shading or lighting treatment. The diameter of flower of 'Dream Round' was 34.1 mm. Numbers of flowers per stem and petals per flower of 'Dream Round' were 12.8 and 26.1, respectively. Its leaf color was green (Green Group 147A) and plant height was 92.4 cm. Days to flowering of 'Dream Round' under the short day treatment was about 54 in spring, and diameter of flower of 'Dream Round' was 34.2 mm in the summer. The vase life of 'Dream Round' was 21.7 days in autumn.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.1
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• pp.41-49
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• 2016

This study was conducted to evaluate maize downy mildew resistance using spreader row technique in Cambodia. A total of forty maize lines were used in this experiment. Seven Korean varieties and seven breeding lines showed high infection rates (80~100%) and highly susceptible (HS) to downy mildew disease in both spring and fall. Also most of nested association mapping (NAM) parent lines were highly susceptible (HS). Meanwhile three inbred lines, Ki3, Ki11, and CML228, showed highly resistant (HR) or resistant (R) in spring and moderately resistant (MR) in fall. These three lines were already known as resistant inbred lines against downy mildew disease. It appears that spreader row technique was suitable for selection of maize downy mildew resistance in Cambodia. The incidence of downy mildew was influenced by weather conditions, especially relative humidity and temperature. Among several inoculation methods to screen for downy mildew resistance, this spreader row technique is effectively and easily used in the field of Southeast Asia.

• Journal of KIISE:Information Networking
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• v.33 no.4
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• pp.289-298
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• 2006

Algorithms for application-level multicast often use trees to deliver data from the source to the multiple receivers. With the tree structure, the throughput experienced by the descendant nodes will be determined by the performance of the slowest ancestor node. Furthermore, the failure of an ancestor node results in the suspension of the session of all the descendant nodes. This paper focuses on the transmission of data using multiple virtual forwarders, and suggests a scheme to overcome the drawbacks of the plain tree-based application layer multicast schemes. The proposed scheme elects multiple forwarders other than the parent node of the delivery tree. A receiver receives data from the multiple forwarders as well as the parent node and it can increase the amount of receiving data per time unit. The multiple forwarder helps a receiver to reduce the impact of the failure of an ancestor node. The proposed scheme suggests the forwarder selection algorithm to avoid the receipt of duplicate packets. We implemented the proposed scheme using MACEDON which provides a development environment for application layer multicast. We compared the proposed scheme with Bullet by applying the implementation in PlanetLab which is a global overlay network. The evaluation results show that the proposed scheme enhanced the throughput by 20 % and reduced the control overhead over 90 % compared with Bullet.

• Journal of Families and Better Life
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• v.1 no.2
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• pp.89-106
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• 1983

After accepting the modernized western culture, we, the Koreans, had traditionally patriarchal valuation on the family life, which has been changed into modernize on in these days. Under these circumstances, we examined into family consciousness of workmen, office workers and student of universities in Pusan with questioning papers by inquiring their general views of family, marriage, family planning , and inheritance. The results obtained can be summarized as follows. 1. General views of family 1)Most of them prefer nuclear family to gross one in structure of family. In decision of family's affairs, the lower educated persons want an unilateral relationship, which means unconditional obdience to their parent's opinion. they are more concerned about the profit of the family than that of individual. on the other hand, they want 2-generations nuclear family system. 2) Concerning children's future affairs, they want compromising method. 2. Views of Marriage 1) Date with the other sex motivated their desire to improve social adaptation and social association. 17-19 year old students regard date as a preparatory stage of marriage. They consider it most desirable date to enjoy free conversation each other. They hope their date partners are high educated. 2) The conditions of mate selection are in order character, health, vitality in living, appearance and education. The less educated placed an emphasis on vitality in living. 3) They are not much interested in marital harmony. If parents are against their marriage an account of bad marital harmony, they will take into consideration about it. 4) They wish to keep purity before marriage, as possible. They want engagement period of 6 months. Any agreeable reasons shall compel them to break off their appointment. 5) they consider it ideal for mate's age to be 26-30 years old, and also think it affirmative to follow their parent's agreement in marriage. It is considerable that they put off their marriage only because they have lots of work to do before marriage. 6)Marriage declaration is to be made on the wedding day. It still exists that they don't want to marry when they are inth same surname and family tree. But it is clear that they don't regard it as the reason of breaking off the betrothal. 3. Family Planning 1) They are willing to agree to the campaign "just two is enough". They want a son and a daughter. Even though they have two daughters, they won's bear child to get son. 2) the lower educated persons are ignorant of the method of birth control. 4. Inheritance 1)Most of them say householder inheritance is to be kept up continuously. It is reasonable that anyone who can afford to perform religious service should bear it responsibility. 2)They don't want the difference in inheritance as the conscious to the conscious of the equality of the sexes spread widely into our society, but it is worthy of notice that some of them still don't mind unequal treatment. 3) When they have no child, the property inheritances are in order his wife and his parents. According to above mentions, we conclude like this: Their consciousness of marital harmony, marriage, family planning and inheritance shows definitely passive rationism in the transitional stage which is mixed with western individualism and traditional feudalism. On account of being lack of steady fast self-conscience, they can not make their positive reaction on anything. Finally, we should make every possible efforts to have our firm self-conscience through the re-education.