• The Korean Journal of Zoology
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• v.28 no.3
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• pp.194-208
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• 1985

The ultrastructure of the integumental epidermis of Korean planaria (Dugesia japonica) is studied by light microscope, scanning electron microscope and transmission electron microscope. The planaria has mono-layered integumental epidermis in which most of cells exhibit irregularly columnar shape. The epidermal cells of the integument are classified into six types on the basis of cytochemical and ultrastructural characteristics. 1) Ciliated epithelial cells: These cells have cilia in their free surfaces. The axonemes of cilia exhibits fundamental 9+2 microtubular pattern. 2) Eosinophilic cells: These cells contain a few large eosinophilic granules. The core of eosinophilic granule is consisted of sparsely dispersed fibrillar structures in relatively electron-lucent ground material. 3) Mucous cells: These cells are filled with irregularly shaped, PAS-positive mucous granules which have an average size of $0.8\\times0.3 \\muM$. 4) Rhabdite-forming cells: These cells possess a few strongly-eosinophilic large rhabdite granules. The rhabdite granules are synthesized either in the rhabdite-forming cells which constitute integumental epidermis or in the corresponding cells which are developed in the parenchyma and later transferred to epidermal cells of integumental epidermis through basement membrane. 5) A-type of basophilic granule cells: These granule cells possess round or irregularly-shaped granules which are strongly stained with Alcian blue. These electron-dense granules have an average size of $1.5\\times1.0 \\muM$. This type of cells is derived from parenchymal tissue. 6) B-type of basophilic granule cells: These basophilic granule cells with PAS-positive granules, are found in the epidermis of lateral body wall. The granules, which are about $0.7\\times0.4 \\muM$ in size, occupying most part of this cell type are originated from the parenchyma.

• Korean Journal Plant Pathology
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• v.10 no.2
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• pp.83-91
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• 1994

Stem tissues of tomato plants (cv. Kwanyang) inoculated with Phytophthora capsici were examined by light and electron microscopy to compare early cytological differences between comaptible and incompatible interactions of tomatoes with the fungus. Twenty four hours after inoculation, the compatible isolate S 197 colonized severely the epidermis, cortex, and xylem vessels of stem tissue, whereas only few fungal cells colonized the stem tissues inoculated with the incompatible isolate CBS 178.26. Fragmented plasma membrane, distorted chloroplast, degraded cell wall, remnants of host cytoplasm were early ultrastructural features of the damaged host cell observed both in the compatible and incompatible interaction, a number of vesicles were distributed in the space between fungal cell walls and plasma membrane. The degradation of host cell walls by P. capsici was more pronounced in the compatible than the incompatible interactions. The incompatible interactions of tomato cells with P. capsici were characterized by formation of host cell wall apposition in the cortical parenchyma cells, indicating that the apposition of electron-dense material from the host cell walls may function as a plant defense reaction to the fungus. The fungal cells encased by wall appositions had abnormal cytoplasm and separated plasma membranes. The haustorium which formed from the fungal hyphae did not further penetrate through the host wall apposition and cytoplasmic aggregation, especially in the incompatible reactions. In contrast, the haustorium of the compatible isolate S 197 was not encased by wall appositions.

• Journal of the Korea Furniture Society
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• v.29 no.1
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• pp.1-7
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• 2018

A study was carried out to observe the 1% aqueous safranine solution flow speed in longitudinal and radial directions of softwood Metasequoia glyptostroboides, diffuse-porous wood Anthocephalus cadamba and ring-porouswood Fraxinus rhynchophylla. In radial direction, ray cells and in longitudinal direction, tracheids, vessel and wood fiber were considered for the measurement of liquid penetration speed at less than 12% moisture contents (MC). The length, lumen diameter, pit diameter, end wall pit diameter and the numbers of end wall pits determined for the flow rate. The liquid flow in the those cells was captured via video and the capillary flow rate in the ones were measured. Vessel in hardwood species and tracheids in softwood was found to facilitate prime role in longitudinal penetration. Anatomical features like the length and diameter, end-wall pit numbers of ray parenchyma were found also responsible fluid flow differences. On the other hand, vessel and fiber structure affected the longitudinal flow of liquids. Therefore, the average liquid penetration depth in longitudinal tracheids of Metasequoia glyptostroboides was found the highest among all cells considered in Anthocephalus cadamba and Fraxinus rhynchophylla In radial direction, ray parenchyma of Metasequoia glyptostroboides was found the highest depth and the one of Fraxinus rhynchophylla was the lowest. The solution was penetrated lowest depth in the wood fiber of Fraxinus rhynchophylla. The large vessel of Fraxinus rhynchophylla was found the lowest depth among the vessels. The solutin was penetrated to the wood fiber of Anthocephalus cadamba higher than the one of Fraxinus rhynchophylla.

• Journal of the Korea Furniture Society
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• v.28 no.1
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• pp.88-93
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• 2017

A study was carried out to observe the 1% aqueous safranine solution flow speed in longitudinal and radial directions of softwood Larix kaempferi (Lamb.)Carriere, diffuse-porous wood Betula davurica Pall.. and ring-porouswood Castanea crenata S.etZ. In radial direction, ray cells and in longitudinal direction, tracheids, vessel and wood fiber were considered for the measurement of liquid penetration speed at less than 12% moisture contents (MC). The length, lumen diameter, pit diameter, end wall pit diameter and the numbers of end wall pits determined for the flow rate. The liquid flow in the those cells was captured via video and the capillary flow rate in the ones were measured. Vessel in hardwood species and tracheids in softwood was found to facilitate prime role in longitudinal penetration. Anatomical features like the length and diameter, end-wall pit numbers of ray parenchyma were found also responsible fluid flow differences. On the other hand, vessel and fiber structure affected the longitudinal flow of liquids. Therefore, the average liquid penetration depth in longitudinal tracheids of Larix kaempferi was found the highest among all cells considered in Betula davurica and Castanea crenata, In radial direction, ray parenchyma of Larix kaempferi was found the highest depth and the one of Betula davurica was the lowest. The solution was penetrated lowest depth in the wood fiber of Castanea crenata. The large vessel of Castanea crenata was found the lowest depth among the vessels. The solutin was penetrated to the wood fiber of Betula davurica higher than the one of Castanea crenata.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.06b
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• pp.373-379
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• 2006

Fiber characteristics and fiber distribution of thermomechanical pulp(TMP), bisulfite chemithermomechanical pulp(bisulfite CTMP), neutral sulfite chemithermomechanical pulp(neutral sulfite CTMP) from kenaf(Hibiscus cannabinus L., Malvaceae) cultivar Tainug-2 cultivated in the reclaimed land of Korea were examined to use effectively nonwood fibers as an alternative raw material sources for papermaking. Yields of TMP and CTMP from kenaf were lower than those of TMP from hardwoods and CTMP from softwoods and hardwoods. Bark fibers of kenaf cultivar Tainung-2 ranged 2.04 to 2.30 mm long and $18.7{\sim}19.7{\mu}m$ width. Core fibers averaged 0.63 to 0.80 mm long and $29.5{\sim}31.4{\mu}m$ wide. Coarseness of bark fiber was higher than that of core fiber, and fiber from TMP were higher than those from both bisulfite CTMP and neutral sulfite CTMP. Curl indexes of bark fibers were higher than those of core fibers. However curl indexes were not significantly affected by the pulping conditions. Short fiber distributions were higher in core fibers from TMP and CTMP and long fiber distributions were higher in bark fibers. There was no significant difference in fiber distribution of whole and core fibers obtained from TMP and CTMP, Fibers from neutral sulfite CTMP, however, exhibited a little higher long fiber distribution. Distinct difference in anatomical characteristics was found between core and bast fibers of kenaf plant. Parenchyma cell, pith parenchyma cell and vessel were observed in core fibers and bast fiber in bast sections.

• Applied Microscopy
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• v.25 no.2
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• pp.39-52
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• 1995

Pinealocytes in the lower vertebrate are known to have photoreceptive function. These photoreceptor cells have been characterized morphologically in various species of lower vertebrates. No such ultrastructural studies, however, were reported in fresh water turtle. The purpose of this study is to characterize the pinealocytes and the phylogenetic evoluton of these cells is discussed in terms of functional analogy. I. Light microscopy: The pineal body was divided into incomplete lobules by connective tissue septa containing blood vessels, and parenchymal cells were arranged as irregular cords or follicular pattern. In the lobules, glandular lumina were present and contained often densely stained materials. II. Electron microscopy: The pineal parenchyma had three categories of cells: photoreceptor cells, supportive cells and nerve cells. The photoreceptor cells had darker cytoplasm compared to the supportive cells, and the enlarged apical cytoplasm(inner segment) containing abundant mitochondria and dense cored vescles protruded into the glandular lumen in which lamellar membrane stacks(outer segment), dense membranous materials, and cilia were present. Some of these lamellated membrane stacks appeared to be dege-nerating while others were apparently newly formed. Constricted neck portion of the photoreceptor cells contained longitudinally arranged abundant microtubules. centrioles and cross-striated rootlets. Cell body had well developed Golgi apparatus, abundant mitochondria, dense granules($0.5{\sim}1{\mu}m$), dense cored vesicles($70{\sim}100nm$), and rough endoplasmic reticulum occasionally with dense material within its cisterna. Basal portion of the photoreceptor cells had basal processes often with synaptic ribbons, which terminate in the complicated zone of cellular and neuronal processes. Synatpic ribbons often made contact with the nerve processes and the cell processes of neighboring cells. In some instances, these ribbons were noted free within the basal process and were also present at the basal cell mem-brane facing the basal lamina. Obvious nerve endings with clear and dense cored vesicles were observed among the parenchymal cells. Photoreceptor cells of the snapping turtle pineal body were generally similar in fine structure to those of other lower verterbrates reported previously, and suggested to have both photoreceptive and secretory functions which were modulated by pinealofugal and pinealopedal nerves. The supportive cells were characterized by having large dense granules($0.3{\sim}1{\mu}m$), abundant ribosomes, well developed Golgi apparatus and rough endoplasmic reticulum. These cells were furnished with microvilli on the luminal cell surfaces, and often had centrioles, striated rootlets, abundant filaments especially around the nucleus, and scattered microtubules. Some supportive cells had cell body close to the lumen and extended a long process reaching to basal lamina, which appeared to be a glial cell. Nerve cells within the parenchyma were difficult to identify, but some large cells located basally were suspected to be nerve cells, since they had synaptic ribbon contact with photoreceptor cells.

• Journal of Life Science
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• v.9 no.2
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• pp.127-135
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• 1999

Gentiana scabra Bunge var buergeri Max, can be widely used such as cut-flower, ornamental and medicinal plant among the native plants. Microscopic and Scanning electron microscopic observation plant were carried out to estimates the characteristics correlated with germinability and germination process, and the results were as follows. The seeds were germinated just after harvest, and it means that there is no or little dormancy of the seeds. Matured seeds showed higher germinability than those of immatured. As the storage period was longer, immatured seeds showed shorter longevity than those of matured. The average longevity was about 190 days. According to the observation results of morphological changes during the germination, seed coat was expanded with water absorption immediately after seeding, and radicals were observed 2 days after seeding. Root hairs were formed behind the growing tip of the root on 4 days after seeding and cotyledon were unfolded on 6 days after seeding. In the microscopic observation of leaf tissues, it could be easily distinguished between midrib, and leaf blade, and also observed upper epidermis, palisade parenchyma and spongy parenchyma consisted as a sing1e layer. Palisade parenchyma was consisted of a single layer of cell. Stomata were restricted lower surface, arranged as anamocytic type, and surrounded by guard cells. In the microscopic anatomy of stem were consisted of cortical layer, forming a single layer of epidermis and parenchyma, and vascular bundle which was consisted of continuous cylinder of vascular tissues.

• Journal of the Korean Wood Science and Technology
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• v.42 no.2
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• pp.138-146
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• 2014

Stem and root of Lindera erythrocarpa were described and compared in the wood anatomical aspects. Root wood appeared to differ from stem wood in the qualitative features of growth ring boundary, extraneous materials in vessel element and ray parenchyma cell, outline of ray, and sheath cell. In the quantitative features, there were differences between these two tissues in vessels per square millimeter, tangential diameter of vessel lumina, length of vessel element, and width of ray. These wood anatomical differences between stem above ground and root below ground were thought to be attributed to their different growth environments.

• Journal of Plant Biology
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• v.33 no.1
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• pp.31-40
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• 1990

Procambium developed in the histogenetical zone below the marginal meristem. The development of procambium is correlated with the developmental stage of the leaf. As long as the marginal cells are crescent-shaped, the first-order procambium develops. When the marginal cell becomes wedge-shaped, the higher order procambium appears. The reticulated venation is developed through forking and anastomosis. The meshes of the first order enlarge in the process of leaf growth and the meshes of the second and third order develop in the meshes of the first-order through the differentiation of the residual meristem. Therefore, the venation is hierachically arranged and is as recognizable from the thickening of the veins. The outermost vein is produced parallel to the leaf margin, in which the differences between the costal and intercostal parts of the marginal meristem are removed. The endodermis and pericycle differentiate from the same mother cell. The procambium and sclerenchyma originate from a common source during the first developmental stage. A small cellular cluster lies within the parenchyma at the upper and lower sides of the procambial trace and differentiates into sclerenchyma.

• Korean Journal of Plant Resources
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• v.32 no.3
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• pp.237-243
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• 2019

Soybean is the one of recalcitrant legume species for shoot induction. Shoot regeneration via direct organogenesis was investigated in five soybean cultivars, 'Dawon', 'Pungsan', 'Daewon', 'Taekwang' and 'Chongdoo 1' by using cotyledonary node explants. Out of 5 soybean cultivars, an efficient shoot regeneration condition was developed in the two soybean cultivars, 'Dawon' and 'Pungsan'. When various kinds of plant growth regulators with different concentration were estimated, the optimum medium condition for shoot induction in both soybean cultivars was MS + B5 vitamin supplemented with BA at concentration 2 mg/L. In addition, shoot formation efficiency was increased with 97.09% and 93.88% by the pretreatment of BA onto the explants before in vitro culture in both cultivars. Shoot induction in 'Dawon' cultivar was originated from epidermal tissue and sub-epidermal layers when histological changes were investigated under shoot regeneration after culturing cotyledonary node segments on shoot induction medium for 0 to 21 days. Especially, cell dedifferentiation was observed from parenchyma cells to meristematic cell in 3-day cultured segments.