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Electron Microscopic Studies on Olfactory Bulbs in the Vertebrates by Phylogenetics (계통발생에 따른 척추동물의 뇌후구에 대한 전자현미경적 연구)

  • Choi, W.B.;Chung, Y.H.;Seo, J.E.
    • Applied Microscopy
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    • v.15 no.2
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    • pp.31-68
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    • 1985
  • Authors are trying to unveil the ultrastructural organization of olfactory bulb, which has been summerized under light microscopic level or communicated only in some detail in different view point until now. For the critical point of view, since the phylogenetical approach will give the ultimate value in the correlative study between structural and functional bases (Brodal, 1969), the present study was carried out light and electron microscopic analyses of the structures of the neurons and synaptic organizations in olfactory bulbs from different animals in phylogenetical scale. We selected each one species from five animal classes: the house rabbit(Oryctolagus cuniculus var. domesticus [Gmelin]) from Mammalia, the domestic fowl (Gallus gallus domesticus Brisson) from Aves, the viper (Agkistrodon hylys [G.P. Pallas]) from Reptilia, a frog (Bombiana orientalis Boulenger) from Amphibia and the crussian carp (Carassius carassius [Linne]) from Pisces. For light microscopic study, samples were fixed in 10% formalin and paraffin sections were stained with hematoxylin-eosin. For the electron microscopic study, the tissues were fixed by perfusion through the heart or immersion with 1% paraform-aldehyde-glutaraldehyde mixture (phosphate buffer, pH 7.4), and final tissue block trimmed under dissecting microscope were osmicated (1% OsO4), they were embedded in Araldite or Epon 812, and ultrathin sections were made by LKB-V ultratome following the inspection of semi-thin sections stained with toluidine blue-borax solution. Ultra-thin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100CX electron microscope. We have summerized our morphological analyses as follows: 1. The olfactory bulb of rabbit, viper and frog shows the eight layers of fila olfactoria, glomerular, external granular, external plexiform, mitral cell, internal plexiform, internal granular, medullary but domestic fowl shows the five layers of glomerular, fibrillar, mitral, granular and medullary and the three layers of fibrilla, glomerular and medullary in crussian carp. The sharpness of demarcation between the layers shows deferential tendency according to phylogenetical order. 2. Mitral cells of vertebrate have large triangular or oval shape with spherical nuclei which contain not so much chromatin. The cytoplasm contains numerous cell organelles, of which Nissl's bodies or granular endoplasmic reticula arranged as parallel strands. Development of granular endoplasmic reticula were declined as the phylogentical grade is going lower. 3. Tufted cells of all animal are mostly spindle or polygonal contour and contain oval nuclei which located in periphery of cytoplasm. The nuclei of rabbit, fowl, viper and frog has relatively space chromatin, but a nucleus of crussian carp contain irregularly aggregated chromatin in karyoplasm. Their cytoplasmic volume and cell organelle contents are in between those of mitral cell and granular cell. They contain moderate amount of mitochondria, granular endoplasmic reticula, a few Golgi complex, polysomes, lysosome, etc. 4. Granule of cells of all the vertebrate amimals studied exhibit similar features; cells and their dense nuclei show spherical or oval contour, and they have the thin rim of cytoplasm which contain only a few cell organelles. 5. In rabbit, the soma of mitral cells were in contact with boutons with two types of synaptic vesicles, that is, round and flat vesicles, especially flat vesicles in boutons were showing reciprocal synapses. However, in domestic fowls, vipers, frogs and crussian carps, there were found boutons showing only spherical synaptic vesicles. 6. The boutons containing round synaptic vesicles were made contact with the some of tufted cell of olfactory bulb in the rabbits, fowls, vipers and frogs, but no synaptic boutons were observed in soma of tufted cells in crussian carps. In the frogs, there were observed dendrites were contact with the soma of tufted cells. 7. In the neuropils of plexiform, granular and glomerular layers olfactory bulbs in the vertebrate, the synapses were axo-large dendrites, axo-median and small dendrites, dendrodendritic, and axo-axonal contacts. However, in the neuropil of crussian carps, synapses were observed only in glomerular layer.

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Calculation of Expected Sliding Distance of Concrete Caisson of Vertical Breakwater Considering Variability in Wave Direction (파향의 변동성을 고려한 직립방파제 콘크리트 케이슨의 기대활동량 산정)

  • 홍수영;서경덕;권혁민
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.16 no.1
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    • pp.27-38
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    • 2004
  • In this study, the reliability design method developed by Shimosako and Takahashi in 1999 for calculation of the expected sliding distance of the caisson of a vertical breakwater is extended to take into account the variability in wave direction such as directional spreading of waves, obliquity of the deep-water design principal wave direction from the shore-normal direction, and its variation about the design value. To calculate the transformation of random directional waves, the model developed by Kweon et al. in 1997 is used instead of Goda's model, which was developed in 1975 for unidirectional random waves normally incident to a straight coast with parallel depth contours and has been used by Shimosako and Takahashi. The effects of directional spreading and the variation of deep-water principal wave directions were minor compared with those of the obliquity of the deep-water design principal wave direction from the shore-normal direction, which tends to reduce the expected sliding distance as it increases. Especially when we used the field data in a part of east coast of Korea, considering the variability in wave directions reduced the expected sliding distance to about one third of that not considering the directional variability. Reducing the significant wave height calculated at the design site by 6% to correct the effect of wave refraction neglected in using Goda's model was found to be proper when the deep-water design principal wave direction is about 20 degrees. When it is smaller than 20 degrees, a value smaller than 6% should be used, or vice versa. When we designed the caisson with the expected sliding distance to be 30㎝, in the area of water depth of 25 m or smaller, we could reduce the caisson width by about 30% at the maximum compared with the deterministic design, even if we did not consider the variability in wave directions. When we used the field data in a part of east coast of Korea, considering the variability in wave directions reduced the necessary caisson width by about 10% at the maximum compared with that not considering the directional variability, and is needed a caisson width smaller than that of the deterministic design in the whole range of water depth considered (10∼30 m).

Genetic Relationship and Structural Characteristics of the Fe-Ti Ore Body and the Sancheong Anorthosite, Korea (산청 회장암과 철-티탄 광체의 구조적 특징과 발생적 관계)

  • Kang, Ji-Hoon;Lee, Deok-Seon
    • Economic and Environmental Geology
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    • v.47 no.6
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    • pp.571-588
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    • 2014
  • It consists of the Precambrian Jirisan metamorphic complex and Sancheong anorthosite complex and the Mesozoic granitoids which intrude them in the Sancheong area, the Jirisan province of Yeongnam massif, Korea. The study area is located in the western part of the stock-type Sancheong anorthosite complex. We performed a detailed fieldwork on the Sancheong anorthosite (SA) and Fe-Ti ore body (FTO) which constitute the Sancheong anorthosite complex, and reinterpreted the origin of FTO foliation and the genetic relationship between them from the foliations, shear zones, occurrences of the SA and FTO. The new structural characteristics between them are as follows: the multilayer structures of FTO, the derived veins of straight, anastomosing uneven types and block structures related to the size reduction of SA, the gradual or irregular boundaries of SA blocks and FTO showing bulbous lobate margins and comb structures, the FTO foliation and linear arrangements of flow occurrence which is not ductile shear deformation, the discontinuous shear zone of SA, the orientation of FTO foliations parallel to the boundaries of SA blocks, the predominance of FTO foliations toward the boundaries of SA blocks and being proportional to the aspect ratio of plagioclase xenocrysts and SA xenoblocks, and the flow folding structures of FTO foliation. Such field evidences indicate that the SA is not fully congealed when the FTO is melt and the fracturing of partly congealed SA causes the derived veins of FTO and the size reduction of SA. Also the gradual or irregular boundaries of SA blocks and FTO result from the mutual reaction between the not fully congealed SA blocks and the FTO melt, and the FTO foliation is a magmatic foliation which was formed by the interaction between the FTO melt and the partly congealed SA blocks. Therefore, these suggest that the SA and FTO are not formed from the intrusion of different magmas in genesis and age but from a coeval and cogenetic magma through multiple fractionation. We predict that the FTO will show an very irregular occurrence injected along irregular fractures, not the regular occurrence like as the intrusive vein and dike. It can be applied to the designing of Fe-Ti mineral resource exploration in this area.

A study on dosimetric comparison of craniospinal irradiation using tomotherpy and reproducibility of position (토모테라피를 이용한 뇌척수조사의 선량적 비교와 자세 재현성에 대한 고찰)

  • Lee, Heejeong;Kim, Jooho;Lee, Sangkyu;Yoon, Jongwon;Cho, Jeonghee
    • The Journal of Korean Society for Radiation Therapy
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    • v.29 no.1
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    • pp.69-76
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    • 2017
  • Purpose: The purpose of this study was to verify dosimetric results and reproducibility of position during craniospinal irradiation (CSI) using tomotherapy (Accuray Incorporated, USA). Also, by comparing with conventional CSI Technique, we confirmed the efficiency of using a Tomotherapy. Materials and Methods: 10 CSI patients who get tomotherapy participate. Patient-specific quality assurances (QA) for each patient are conducted before treatment. When treating, we took Megavoltage Computed Tomography (MVCT) that range of head and neck before treatment, L spine area after treatment. Also we conducted in-vivo dosimetry to check a scalp dose. Finally, we made a 3D conventional radiation therapy(3D-CRT) of those patients to compare dosimetric differences with tomotherapy treatment planning. Results: V107, V95 of brain is 0 %, 97.2 % in tomotherapy, and 0.3 %, 95.1 % in 3D-CRT. In spine, value of V107, V95 is 0.2 %, 18.6 % in tomotherapy and 89.6 %, 69.9 % in 3D-CRT. Except kidney and lung, tomotherapy reduced normal organ doses than 3D-CRT. The maximum positioning error value of X, Y, Z was 10.2 mm, -8.9 mm, -11.9 mm. Through in-vivo dosimetry, the average of scalp dose was 67.8 % of prescription dose. All patient-specific QA were passed by tolerance value. Conclusion: CSI using tomotherapy had a risk of parallel organ such as lung and kidney because of integral dose in low dose area. However, it demonstrated dosimetric superiority at a target and saved normal organ to reduce high dose. Also results of reproducibility were not exceeded margins that estimated treatment planning and invivo dosimetry showed to reduce scalp dose. Therefore, CSI using tomotherapy is considered to efficient method to make up for 3D-CRT.

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On the Utilization of Inactive BHC isomers -Synthesis of 3-(2,4,5-trichlorophenyl)-1-methyl urea as a herbicide- (BHC 이성질체(異性質體)의 활용(活用)에 관(關)한 연구(硏究) -제초제(除草劑)로서 3-(2,4,5-trichlorophenyl)-1- methyl urea의 합성(合成)-)

  • Lee, Kyu-Seung;Park, Chang-Kyu
    • Applied Biological Chemistry
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    • v.22 no.2
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    • pp.109-122
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    • 1979
  • Present study was carried out to reduce residual toxicity of BHC insecticides inherent in the organochlorine pesticides. For This end, r-isomer, the most potent insecticidal component among the BHC stereoisomers, was isolated and thus fortified by means of solvent precipitation. In parallel, 3-(2,4,5-trichlorophenyl)-1-methyl urea was prepared in good yield from technical BHC via 1,2,4-trichlorobenzene, 1,2,4,-trichloronitrobenzene, and 2,4,5-trichloroaniline. In addition, certain merit of the compound which make it possible to use as a herbicide is discussed. The results are summarized as follows; 1. Recrystallizing technical BHC from methanol-water binary solvent system, r-isomer was enriched to 49.7% at 95% recovery of r-isomer. 2. By partitioning technical BHC in 85% of methanolic solution into chloroform, r-isomer was fortified to 89.6% at 90.5% recovery of r-isomer. 3. Yield of 1,2,4-trichlorobenzene from technical BHC was greatly dependent upon concentration of alkalies and to less degree on the alkalies. 4. Surfactants, in particular cationic a quartenary ammonium salt, increased yield of 1,2,4-trichlorobenzene from technical BHC by alkaline hydrolysis. 5. Conversion of 1,2,4-trichlorobenzene to 2,4,5-trichloronitrobenzene was effected almost quantitatively utilizing $HNO_3-H_2SO_4$ nitrating agent at low temperature. 6. Yield of 91.4% was observed for the synthesis of 2,4,5-trichloroaniline by reducing 2,4,5-trichloronitrobenzene in the presence of iron turning and hydrochloric acid. 7. Overall yield based on BHC of 3-(2,4,5-trichlorophenyl)-1- methyl urea was 60.8%. 8. Inhibition effects, both germination and growth, 3-(2,4,5-trichlorophenyl)-1-methyl urea on several crops were found comparable to or more potent than those of $linuron{\circledR}\;and\;diuron{\circledR}$. In addition, it was also noted that susceptibility to the prepared compound depended upon the crops as well as specific part (shoots, roots) of the plant exposed to the chemicals.

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Measurement of shoulder motion fraction and motion ratio (견관절 운동 분율의 측정)

  • Kang, Yeong-Han
    • Journal of radiological science and technology
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    • v.29 no.2
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    • pp.57-62
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    • 2006
  • Purpose : This study was to understand about the measurement of shoulder motion fraction and motion ratio. We proposed the radiological criterior of glenohumeral and scapulothoracic movement ratio. Materials and Methods : We measured the motion fraction of the glenohumeral and scapulothoracic movement using CR(computed radiological system) of arm elevation at neutral, 90 degree, full elevation. Central ray was $15^{\circ},\;19^{\circ},\;22^{\circ}$ to the cephald for the parallel scapular spine, and the tilting of torso was external oblique $40^{\circ},\;36^{\circ},\;22^{\circ}$ for perpendicular to glenohumeral surface. Healthful donor of 100 was divided 5 groups by age(20, 30, 40, 50, 60). The angle of glenohumeral motion and scapulothoracic motion could be taken from gross arm angle and radiological arm angle. We acquired 3 images at neutral, $90^{\circ}$ and full elevation position and measured radiographic angle of glenoheumeral, scapulothoracic movement respectively. Results : While the arm elevation was $90^{\circ}$, the shoulder motion fraction was 1.22(M), 1.70(W) in right arm and 1.31, 1.54 in left. In full elevation, Right arm fraction was 1.63, 1.84, and left was 1.57, 1.32. In right dominant arm(78%), $90^{\circ} and Full motion fraction was 1.58, 1.43, in left(22%) 1.82, 1.94. In generation 20, $90^{\circ} and Full motion fraction was 1.56, 1.52, 30' was 1.82, 1.43, 40' was 1.23, 1.16, 50' was 1.80, 1.28, 60' was 1.24, 1.75. There was not significantly by gender, dominant arm and age. Conclusion : The criterior of motion fraction was useful reference for clinical dignosis the shoulder instability.

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Policy Suggestions Regarding to Soil Quality Levels in Korea from a Comparison Study of the United States, the United Kingdom, Germany, the Netherlands, and Denmark's Soil Quality Policies (토양질 기준에 관한 주요 외국 정책의 비교분석을 통한 우리나라의 토양질 기준 개념설정과 적용)

  • Park Yong-Ha;Yang Jae-E;Ok Yong-Sik
    • Journal of Soil and Groundwater Environment
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    • v.10 no.4
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    • pp.1-12
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    • 2005
  • Policies regarding to soil quality of the United States, the United Kingdom, the Netherlands, Germany, and Demark were analyzed to suggest Korean policy for improving soil quality concept and it's implementation. All countries met four criteria: I) Soil quality levels of contaminants are indebt to concept of contaminant risk to recipients (human and ecosystem); ii) Any soil quality value can't be a magic number to determine whether a site is contaminated or not. To determine risk of sites, risk assessment of the sites should be followed; iii) Concentrations of contaminants of sites are not always significantly certain to risk of human and ecosystem of the sites; and iv) Soil quality levels are adopted based on land uses and plans. Considering our rooms to improve policies and analysis of the other country reports on their legislations about soil quality levels, our policy implementation could be approached from these directions: i) Our concept for soil quality levels needs to develop in scientific and rational. ii) Soil quality levels and risk assessment should be implemented as determining tools of site contamination in parallel, and iii) Soil quality levels depending on land uses and plans should be developed in debt with rational and scientific concept of risk. Increasing efficacy of Korea policy regarding the soil quality levels would be in dept to applying concepts of SCL (Soil Contamination Level) and SRL (Soil Regulatory Level) developed, implementing soil quality levels and risk assessment of contaminated sites in conjunction, and classifying three distinctions of land uses based on sensitiveness of recipients (human and ecosystem) to contaminants in soil in this research.

Lateral Strength of Double-Bolt Joints to the Larix Glulam according to Bolt Spacing (볼트 간격에 따른 낙엽송 집성재 이중 볼트접합부의 전단강도)

  • Kim, Keon-Ho;Hong, Soon-Il
    • Journal of the Korean Wood Science and Technology
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    • v.36 no.3
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    • pp.1-8
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    • 2008
  • The lateral strength test of bending type was done to investigate the lateral capacity of the double bolt connection of domestic larix glulam according to bolt spacing. In the shear specimen, which is bolted connection in the inserted plate type, the hole of bolt was made, changing the diameter of bolt (12 mm and 16 mm), the number of bolt (single bolt : control and double bolt), the direction of bolt row (in parallel to grain : Type-A and in perpendicular to grain : Type-B) and the bolt spacing (Type-A : 4 d and 7 d and Type-B : 3 d and 5 d). Lateral capacity and failure mode of bolt connection were compared according to conditions. In prototype design (KBCS, 2000), the reduction factor of the allowable shear resistance that the bolt spacing is reduced was calculated. The results were as follows. 1) Bearing stress per bolt in the single and double bolt connection of Type-A was directly proportional to bolt diameter and bolt spacing. Bearing stress of Type-B decreased as bolt diameter was increased, and decreased by 2~10% when bolt diameter was increased. 2) In the single bolt connection and the double bolt connection of Type-A, the splitted failure was formed in the edge direction. When the bolt spacing was 3 d in Type-B, bolt was yielded more in the part of tension than in the part of compression, and the splitted failure started at the bolt in the part of tension. In the 5 d spacing specimen, the bolt in the part of tension was yielded similarly to bolt in the part of compression, and the splitted failure started in the part of compression. 3) In the prototype design, the reduction factor was calculated by non-dimensionizing the yielding load in the standard of bolt spacing (Type A : 7 d and Type B : 5 d). In 12 mm bolt connection, the reduction factor of bolt spacing 4 d (type-A) and single bolt connection was 0.87 and 0.55, respectively, and the reduction factor of bolt spacing 3 d (Type-B) and single bolt connection was 0.91 and 0.55, respectively. In 16 mm bolt connection, the reduction factor of bolt spacing 4 d (type-A) and single bolt connection was 0.96 and 0.76, respectively, and the reduction factor of bolt spacing 3 d (Type-B) and single bolt connection was 0.91 and 0.77, respectively.

Dose Alterations at the Distal Surface by Tissue Inhomogeneity in High Energy Photon Beam (조직 불균질성에 의한 고에너지 광자선의 선량변화)

  • Kim, Young-Ai;Choi, Tae-Jin;Kim, Ok-Bae
    • Radiation Oncology Journal
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    • v.13 no.3
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    • pp.277-283
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    • 1995
  • Purpose : This study was performed to measure dose alteration at the air-tissue interface resulting from rebuild-up to the loss of charged particle equilibrium in the tissues around the air-tissue interfaces. Materials and Methods : The 6 and 10-MV photon beam in dual energy linear accelerator were used to measure the surface dose at the air-tissue interface The polystyrene phantom sized $25{\times}25{\times}5\;cm^3$ and a water phantom sized $29{\times}29{\times}48\;cm^3$ which incorporates a parallel-plate ionization chamber in the distal side of air gap were used in this study. The treatment field sizes were $5{\times}5\;cm^2,\;10{\times}10\;cm^2\;and\;20{\times}20\;cm^2$. Air cavity thickness was variable from 10 mm to 50 mm. The observed-expected ratio (OER) was defined as the ratio of dose measured at the distal junction that is air-tissue interface to the dose measured at the same point in a homogeneous phantom. Results : In this experiment, the result of OER was close or slightly over than 1.0 for the large field size but much less (about 0.565) than 1.0 for the small field size in both photon energy. The factors to affect the dose distribution at the air-tissue interface were the field size, the thickness of air cavity. and the photon energy. Conclusion : Thus, the radiation oncologist should take into account dose reduction at the air-tissue interface when planning the head and neck cancer especially pharynx and laryngeal lesions, because the dose can be less nearly $29{\%}$ than predicted value.

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Architecture and Depositional Style of Gravelly, Deep-Sea Channels: Lago Sofia Conglomerate, Southeyn Chile (칠레 남부 라고 소피아 (Lago Sofla) 심해저 하도 역암의 층구조와 퇴적 스타일)

  • Choe Moon Young;Jo Hyung Rae;Sohn Young Kwan;Kim Yeadong
    • The Korean Journal of Petroleum Geology
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    • v.10 no.1_2 s.11
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    • pp.23-33
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    • 2004
  • The Lago Sofia conglomerate in southern Chile is a lenticular unit encased within mudstone-dominated, deep-sea successions (Cerro Toro Formation, upper Cretaceous), extending from north to south for more than $120{\cal}km$. The Lago Sofia conglomerate is a unique example of long, gravelly deep-sea channels, which are rare in the modern environments. In the northern part (areas of Lago Pehoe and Laguna Goic), the conglomerate unit consists of 3-5 conglomerate bodies intervened by mudstone sequences. Paleocurrent data from these bodies indicate sediment transport to the east, south, and southeart. The conglomerate bodies in the northern Part are interpreted as the tributary channels that drained down the Paleoslope and converged to form N-S-trending trunk channels. In the southern part (Lago Sofia section), the conglomerate unit comprises a thick (> 300 m) conglomerate body, which probably formed in axial trunk channels of the N-5-trending foredeep trough. The well-exposed Lago Sofia section allowed for detailed investigation of sedimentary facies and large-scale architecture of the deepsea channel conglomerate. The conglomerate in Lago Sofia section comprises stratified conglomerate, massive-to-graded conglomerate, and diamictite, which represent bedload deposition under turbidity currents, deposition by high-density turbidity currents, and muddy debris flows, respectively. Paleocurrent data suggest that the debris flows originated from the failure of nearby channel banks or slopes flanking the channel system, whereas the turbidity currents flowed parallel to the orientation of the overall channel system. Architectural elements produced by turbidity currents represent vertical stacking of gravel sheets, lateral accretion of gravel bars, migration of gravel dunes, and filling of channel thalwegs and scoured hollows, similar to those in terrestrial gravel-bed braided rivers. Observations of large-scale stratal pattern reveal that the channel bodies are offset stacked toward the east, suggestive of an eastward migration of the axial trunk channel. The eastward channel migration is probably due to tectonic tilting related to the uplift of the Andean protocordillera just west of the Lago Sofia deep-sea channel system.

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