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Bacterial Leaf Spot and Dry Rot of Lettuce Caused by Xanthomonas campestris pv. vitians (Xanthomonas campestris pv. vitians에 의한 상추 세균성점무늬병)

  • Lee, Seung-Don;Lee, Jung-Hee;Kim, Yong-Ki;Heu, Sung-Gi;Ra, Dong-Soo
    • Research in Plant Disease
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    • v.13 no.1
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    • pp.66-70
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    • 2007
  • During 1997 and 1998, a new disease of lettuce (Lactuca sativa) was observed on greenhouse-grown plants in Kwangju and Kwangmyung. Lesions on leaves were irregular, small, pale green to black, water-soaked, and 2 to 5 mm in diameter, Coalescing lesions sometimes caused defoliation of older leaves. Isolations made from diseased leaves on yeast extract dextrose calcium carbonate agar yielded nearly pure cultures of a yellow pigmented bacterium typical of a xanthomonad. Two bacterial strains (SL0246 and SL1352) were purified and used for further tests. Pathogenicity of strains was confirmed on 5-week-old lettuce plants injected with bacterial suspensions containing $10^8$ cfu/ml of phosphate buffered saline. The representative Xanthomonas strains isolated from lettuce were compared with a reference strain X. campestris pv, vitians for fatty acid profiles and metabolic fingerprints using GN2 microplate, showing that all outcomes were indistinguishable between the representative and reference strains. This is the first report of bacterial leaf spot and dry rot of lettuce in Korea.

Study on Centyocestus armatus in Korea I. Infection status of Zucco plutupus and Z. temminckii with the metacercariae of C. armatus (Centrocestus armatus에 관한 연구 1. 피라미와 갈겨니의 피낭유충 감염상)

  • Hong, Seong-Jong;U, Ho-Chun;Kim, In-Tae
    • Parasites, Hosts and Diseases
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    • v.27 no.1
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    • pp.41-46
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    • 1989
  • This study was performed to observe the infection rate and infection intensity of fresh water fish such as pale chub (Z. platypus) and dark chub (Z. temminckii) with the metacercariae of C. armatus. The fish were caught in several rivers and streams from June to September, 1988 and examined by artificial digestion technique. Total 370 fish were caught at 19 rivers. The metacercarial (C. armatus) infection rate and average burden in Z. Platypus were 86.8% and 224, and those in Z. tsmminckii were 78.5% and 131. The infection rate and burden per fish were 73.4% and 32 in the fish caught from the Han river, and 88.0% and 44 from the Youngsan river. All fish caught from the Keum and the Tamjin river were infected and their mean metacercarial density was 45 and 59 respectively. The infection rate and density in fish caught from the Seomjin and Nagdong rivers were 100% and 119, and 81.0% and 348 respectively. From this study, it is confirmed that C. armatus is widely distributed along 5 major rivers in Korea.

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A pathological observation on the experimental rabbit viral hepatitis (실험적 토끼 바이러스성 간염의 병리학적 관찰)

  • Jyeong, Jong-sik;Kwon, Young-ran;Lee, Cha-soo;Shin, Tae-kyun
    • Korean Journal of Veterinary Research
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    • v.32 no.4
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    • pp.605-608
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    • 1992
  • This paper dealt with the clinical and pathological observations on the experimental rabbit viral hepatitis. Rabbits with 2-14 months of ages were intramuscularly inoculated with virus suspension. The results were summarized as follows. Ninety percents of experimental rabbits inoculated with virus died within 96 hours postinoculation. Common clinical signs were inappetence, increase in body temperature, depression, mild diarrhea and in three cases, bloody foam from nostrils was recognized. At necropsy, in most of the experimental cases, there were hyperemia or haemorrhages in many organs and pale liver. Intestinal catarrh and retention of turbid urine in urinary bladder were seen in some cases. Histopathologically, liver necrosis was found in all the cases died of this disease. However, there was a difference in the severity of hepatic necrosis. Haemorrhages were Iecognized in lungs, heart, liver, spleen, kidneys and thymus, in order. Liver necrosis was marked even in the cases with no haemorrhage. Perivascular cuffing in brain and catarrhal enteritis in small intestine were seen in many cases. From these results, consistent and primary lesion in this viral disease is hepatitis in susceptible rabbits. It was concluded that rabbit hepatitis virus might have the properties of hepatotropism and consequently induce peripheral necrosis in the liver leading to acute viremia with haemorrhages.

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Physicochemical Properties and Sensory Evaluation of Citrus Unshiu Produced in Cheju (제주산 온주밀감의 특성과 관능평가)

  • Koh, Jeong-Sam;Koh, Jeong-Eun;Yang, Sang-Ho;Ahn, Sung-Ung
    • Applied Biological Chemistry
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    • v.37 no.3
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    • pp.161-167
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    • 1994
  • Soluble solids $(^{\circ}Brix)$, acid content and vitamin C content of Citrus miyakawa unshiu and C. okitsu unshiu juice harvested middle of November 1993 in Seuho-Dong and Topyung-Dong, Seogwipo-si, south area of Cheju were $10.7{\sim}11.0$, 1.04% and $41.19{\sim}44.94\;mg/100\;g$ juice, respectively. Carbohydrate was consisted of about 1/2 sucrose, 1/4 glucose, and 1/4 fructose. Fruit weight, peel thickness, soluble solids, pH, acid content and Brix/Acid ratio had a good correlation in linear function with increasing fruit size, respectively. However, hardness and edible part ratio were not showed correlation with increasing fruit size. Middle size of citrus fruit was favorable to panelists, and the citrus peel color of red yellow was more favorable than pale yellow color in sensory evaluation. Sensory evaluation score showed a linear correlation among citrus fruits of C. okitsu unshiu produced in Namwon-ri, Seuho-Dong, Seogwipo-si, south area of Cheju, and that of Chochun-ri, Ara-Dong, Cheju-si, north area of Cheju, and the score was higher according to citrus fruit produced in optimum cultivation area. With increasing Brix/Acid ratio of citrus juice, high sensory evaluation score was gained in panelists. These data obtained are supposed to be applied to the quality evaluation of Citrus unshiu produced in Cheju.

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Authigenic Phillipsite in Deep-sea Manganese Nodules from the Clarion-Clipperton Fracture Zones, NE Equatorial Pacific (적도 북동 태평양, 클라리온-클리퍼톤 균열대에서 산출되는 망간단괴내의 자생 필립사이트)

  • Lee, Chan Hee;Lee, Sung-Rock
    • Economic and Environmental Geology
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    • v.29 no.4
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    • pp.421-428
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    • 1996
  • The occurrence, optical property, chemical composition, crystal structure and formation environments of the phillipsite within deep-sea manganese nodules were systematically investigated in this study. Phillipsite in manganese nodules occurs in nucleus of nodules along with consolidated bottom sediments, weathered volcanic debris, and interstitial grains in the each layer of manganese encrusts. Phillipsite is predominantly pseudomorphs of volcanic shards, and occurs as white to pale yellow in color lath-shaped and equant crystals. These show aggregations of prismatic, blocky, and bladed of 2 to $20{\mu}m$ long, and 2 to $5{\mu}m$ thick. The simplified average chemical formula of phillipsite is $({Ca_{0.1}Mg_{0.3}Na_{1.1}K_{1.5}})_3{(Fe_{0.3}Al_{4.2}Si_{11.8})O_{32}{\cdot}10H_2O}$ with a very siliceous and alkalic. The $Si/(Al+Fe^{+3})$ ratio is 2.37 to 2.78 and alkalis greatly exceed the divalent exchangeable cations, and Na/K ratio is 0.59 to 0.81. The phillipsite is monoclinic ($P2_l/m$) with the unit-cell parameters, $a=10.005{\AA}$, $b=14.129{\AA}$, $c=8.686{\AA}$, ${\beta}=124.35^{\circ}$, and $V=1013.6{\AA}^3$. Phillipsites in manganese nodules formed apparently authigenically at a temperature less than $10^{\circ}C$, and they crystallized at a pressure of less than 0.7 kb, and pH of about 8 in deep-sea environments.

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Mineralogy and Geochemistry of Green-colored Cr-bearing Sericite from Hydrothermal Alteration Zone of the Narim Gold Deposit, Korea (나림 금광상의 열수변질대에서 산출되는 녹색크롬-견운모의 광물학적 및 지구화학적 특징)

  • Lee, Hyun Koo;Lee, Chan Hee
    • Economic and Environmental Geology
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    • v.30 no.4
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    • pp.279-289
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    • 1997
  • Dark to pale green-colored, Cr-bearing sericites from hydrothermal alteration zone of the Narim gold deposit were investigated mineralogically and geochemically. The alteration zone is composed mineralogically of quartz, carbonate minerals and green sericite with minor amounts of chlorite, barite and sulfide minerals (pyrite, sphalerite, galena). The zone is enriched in As (967 to 1520 ppm), Cu (31 to 289 ppm), Ni (1027 to 1205 ppm), Pb (0.20 to 1.24 wt.%) and Zn (1.03 to 1.07 wt. %) compared with fresh rocks such as granitic gneiss, porphyritic biotite granite and basic dyke. The Cr, probably the chromophore element, is highly enriched in the alteration zone (1140 to 1500 ppm), host granitic gneiss (1200 ppm) and porphyritic biotite granite (1200 ppm). Occurrence and grain size of sericite are diverse, but most of the Cr-bearing sericites (150 to $200{\mu}m$ long and 20 to $30{\mu}m$ wide) occur along the boundaries between ore veins and host rocks (especially basic dyke and granitic gneiss). X-ray diffraction data of the sericite show its monoclinic form with unit-cell parameters of $a=5.202{\AA}$, $b=8.994{\AA}$, $c=20.103{\AA}$, ${\beta}=95.746^{\circ}$ and $V=935.83{\AA}^3$, which are similar with the normal 2M1-type muscovite. Representative chemical formula of the sericite is ($K_{1.54}Ca_{0.03}Na_{0.01}$)($Al_{3.42}Mg_{0.38}Cr_{0.14}Fe_{0.06}V_{0.02}$)($Si_{6.69}Al_{1.31}$)$O_{20}(OH)_4$. The Cr content increases with decrease of the octahedral Al content, and ranges from 0.36 to 2.58 wt.%. DTA and TG curves of the sericite show endothermic peaks at $342^{\circ}$ to $510^{\circ}$, $716^{\circ}$ to $853^{\circ}$ and $1021^{\circ}C$, which are due to the expulsion of hydroxyl group. The total weight loss by heating is measured to be about 8.8 wt. %, especially at $730^{\circ}C$. Infrared absorption experiments of the sericite show broad absorption band due to the O-H bond stretching vibration near the $3625cm^{-1}$, coupled with the 825 and $750cm^{-1}$ doublet. The vibration bands related with the H-O-Al and Si-O-Al bonds occur at $1030cm^{-1}$ and 500 to $700cm^{-1}$, respectively. Based on paragonite content of the sericite, the formation temperature of the Narim gold deposit is calculated to be $220{\pm}10^{\circ}C$.

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Estimation of Optimal Ecological Flowrate of Fish in Chogang Stream (초강천에서 어류의 최적 생태유량 산정)

  • Hur, Jun Wook;Kim, Dae Hee;Kang, Hyeongsik
    • Ecology and Resilient Infrastructure
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    • v.1 no.1
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    • pp.39-48
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    • 2014
  • In order to establish fundamental data for stream restoration and environmental flow, we investigated optimal ecological flowrate (OEF) and riverine health condition in the Chogang Stream, a tributary to Geum River, Korea. The number of fish individuals sampled in this period were 4,669 in 36 species of 9 families. The most abundant species was Korean chub (Zacco koreanus, 34.0%) followed by pale chub (Z. platypus, 22.6%) and Korean shinner (Coreoleuciscus splendidus, 13.3%). Index of biological integrity (IBI) and qualitative habitat evaluation index (QHEI) values decreased from upstream to downstream along the stream. The estimated IBI value ranged from 27.9 to 38.6 with average 32.2 out of 50, rendering the site ecologically fair to good health conditions. OEF was estimated by the physical habitat simulation system (PHABSIM) using the habitat suitability indexes (HSI) of three fish species Z. koreanus, C. splendidus and Pseudopungtungia nigra selected as indicator species. In Z. koreanus, HSI for flow velocity and water depth were estimated at 0.1 to 0.4 m/s and 0.2 to 0.4 m, respectively. In P. nigra, HSI for flow velocity, water depth and substrate size were estimated at 0.2 to 0.5 m/s and 0.4 to 0.6 m and fine gravel to cobbles, respectively. OEF values increasing from up to downstream was found to increase, weighted usable area (WUA) values increased accordingly.

Effects of Handling and Transport Vehicle on Stress and Carcass Quality of Market Pigs (출하취급과 차량형태가 출하돈의 스트레스와 도체품질에 미치는 영향)

  • Kim, Doo-Hwan;Song, Jun-Ik;Jeon, Jung-Hwan
    • Journal of Animal Environmental Science
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    • v.17 no.2
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    • pp.101-106
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    • 2011
  • One hundred and forty-four cross-bred market pigs weighing approximately 110 kg were randomly divided into four groups in a 2 (handling stress; minimal vs stimulated) ${\times}$ 2 (transport vehicle type; enclosed box vs conventional open) factorial arrangement of treatments. The stimulated handling stress group received overally rough handling including electric prod stimulation during loading, transport and lairage at least once at each step. All the animals received 3-h lairage prior to slaughter. Blood and longissimus dorsi muscle (LM) samples were taken at slaughter and after overnight chilling of the carcass, respectively. Plasma concentrations of stress indicators glucose, cortisol, creatine kinase and lactate dehydrogenase were greater in the stimulated vs minimal handling stress group. There were no interactions between the handling stress and transport vehicle type in their effects on these blood variables. The incidence of pale, soft and exudative (PSE) carcass and drip loss of LM, 24-h postmortem LM pH and color including the lightness and redness were not affected by the handling stress and transport vehicle type. In conclusion, results suggest that rough handling inflicts a stimulated stress on the animal, which is manifested by increased blood concentrations of stress indicators, and therefore should be avoided for animal welfare.

Carcass Grade and Characteristics by Carcass Weight and Backfat Thickness of Pigs (돼지의 도체중과 등지방 두께에 따른 도체 등급 및 도체 특성)

  • Kim Gye-Woong;In Byoung-Soon
    • Food Science of Animal Resources
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    • v.26 no.2
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    • pp.183-188
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    • 2006
  • This study was carried out to investigate the carcass grade and characteristics of pigs by carcass weight and backfat thickness. Data used in this experiment were collected and analyzed from a total of 656 pigs composed of 128 gilts and 528 barrows at commercial public slaughter house. The carcass grade of gilts was superior to that of barrow. The carcass weight by gender tended to be heavier in castrated pig than in gilt. Backfat was significantly (p<0.05) thicker in castrated pig than in gilt. Differences between castrated pig and gilt on meat colour, marbling standard, water holding capacity, and marketing age were not significantly found. However, the level of carcass grade was higher ratio in gilt group than in castration. The appearance of percentage of PSE (pale, soft, exudative) of gilt group was superior to that of castrated group. There were significant differences among carcass weight in the marbling score (p<0.05) and water holding capacity (p<0.05). There was significant difference among backfnt thickness in carcass weight (p<0.05), marbling score (p<0.05) and water holding capacity (p<0.05).

A New Record of Tarsonemid Mite, Stenotarsonemus spinki (Acari, Tarsonemidae) and Its Damage on Rice in Korea (한국 미기록 벼먼지응애, Steneotarsonemus spinki와 그 피해)

  • 조명래;김덕수;임대준;나승용;임명순
    • Korean journal of applied entomology
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    • v.38 no.2
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    • pp.157-164
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    • 1999
  • A tarsonemid mite. Stet~rotc~r.sot~rrrs~puin.sk i Smiley 1967. is an important rice pest in major rice producing countries of Thailand. Philippine. China, and Taiwan. S. spinki was found first time in Korea from rice grown in environment controlled greenhouse. Rice fed by the mite showed damage symptoms of deformed panicles and inflorescence, lesions on the inner surface of leaf sheath, and browning of rice hulls. Females of S. .spi~lkwi as 263.0 pni (246.5-284.6f 12.2) in body length and 92.4 pm (79.5- 104.9 t 7.6) in body width. Body was elongate and broadest in region of hysterosoma. Body color was pale brown. Legs were robust except for the legs IV which were typical tarsonemid female legs terminating in a whiplike seta two times the length of the leg. Male of S. spir~kiw as 196.5 pm ( 176.5-222.8 + 15.8) in body length and 109.3 ym (98.6- 1 17.7 + 6.4) in body width. Anterior ends of apodemes 111 were extended further than apodemes IV. Femur IV had large inner median lateral flange. and inner anterior and outer median setae were short about equal length. Tarsal claw was stout and curved ventrally.

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