• Animal cells and systems
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• v.16 no.4
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• pp.321-328
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• 2012

The oily bittering Acheilognathus koreensis is a freshwater species that is endemic to Korea and is experiencing severe declines in natural populations as a result of habitat fragmentation and water pollution. For the conservation and restoration of this species, it is necessary to assess its genetic diversity at the population level. We developed 13 polymorphic microsatellite loci that were used to analyze the genetic diversity of two populations collected from the Kum River and the Tamjin River in Korea. All loci exhibited Mendelian inheritance patterns when examined in controlled crosses. Both populations revealed high levels of variability, with the number of alleles ranging from 3 to 20 and observed and expected heterozygosities ranging from 0.500 to 0.969 and from 0.529 to 0.938, respectively. None of the loci showed significant deviation from Hardy-Weinberg equilibrium, and one pair of loci showed significant linkage disequilibrium after Bonferroni correction. Pairwise $F_{ST}$ and genetic distance estimation showed significant differences between two populations. These results suggest that the microsatellites developed herein can be used to study the genetic diversity, population structure and conservation measure of A. koreensis.

• Animal cells and systems
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• v.15 no.1
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• pp.37-43
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• 2011

The Korean freshwater crayfish, Cambaroides similis, has recently suffered from range reduction and habitat degradation caused by environmental changes and water pollution. For the conservation and restoration of this species, it is necessary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight micro satellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was revealed between any of the locus pairs after Bonferroni correction. From the pairwise Fst results over all samples, higher differentiation between GA-BH population pairs (mean 0.1789) was observed than between GA population pairs (mean 0.0454). This was also supported by Mantel's test showing that the genetic distances of these crayfish populations were significantly correlated with geographic distances. This result may show the regional differentiation caused by restricted gene flow between northern (BH) and southern (GA) populations within Seoul. These micro satellite markers have the potential for use in analyses of the genetic diversity and population structure of C. similis species, with implications for its conservation and management plans.

• YAKHAK HOEJI
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• v.54 no.1
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• pp.49-54
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• 2010

SLC6A19 which reported as a neurotransmitter was composed of seven minisatellites. In previous our study, the minisatellites variants of SLC6A19-MS7 showed the susceptibility for hypertension. When this minisatellte sequences were analyzed using the bioinformatic tool, USF1 (upstream transcription factor 1) was found in this region as a putative transcription factor binding site. USF1 is binding with E-boxes which has a consensus sequence of CACGTG. USF1 is a ubiquitously expressed transcription factor and involved in the transcriptional control of many genes including the molecular pathogenesis of cardiovascular disease. Thus, we investigated that the putative functional relationship between the minisatellites variants and susceptibility for myocardial infarction. A case-control study was performed that compared genomic DNA from 400 controls and 225 cases with myocardial infarction. There were no significant differences observed in the overall allelic distribution of minisatellites between controls and cases, which indicates that this polymorphism is not responsible for myocardial infarction susceptibility. Hence, we analyzed the five different minisatellites alleles from this study and characterized 14 different repeats units (Unit1~Unit14). Then, we evaluated the DNA composition, phylogenic tree, and pairwise distances of its repeats. The variability of each repeats differed from 2.33% to 16%. The phylogenic trees for the four SLC6A19-MS7 minisatellites exhibited very different shapes in their braches and distances, and present most common 8 repeats allele was the longest 14 repeats allele. Therefore, this result may help to understand for the evolutional level of the length of minisatellites.

• Restorative Dentistry and Endodontics
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• v.45 no.2
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• pp.13.1-13.10
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• 2020

Objective: The aim of this study was to evaluate the influence of different modeling agents on the surface microhardness (Vickers hardness number; VHN), roughness (Ra), and color change (ΔE) of a nano-hybrid composite with or without exposure to discoloration by coffee. Materials and Methods: Sixty-four cylinder-shaped nano-hybrid composite specimens were prepared using a Teflon mold. The specimens' surfaces were prepared according to the following groups: group 1, no modeling agent; group 2, Modeling Liquid; group 3, a universal adhesive (G-Premio Bond); and group 4, the first step of a 2-step self-adhesive system (OptiBond XTR). Specimens were randomly allocated into 2 groups (n = 8) according to the storage medium (distilled water or coffee). VHN, Ra, and ΔE were measured at 24 hours, 1 week, and 6 weeks. The Kruskal-Wallis test followed by the Bonferroni correction for pairwise comparisons was used for statistical analysis (α = 0.05). Results: Storage time did not influence the VHN of the nano-hybrid composite in any group (p > 0.05). OptiBond XTR Primer application affected the VHN negatively in all investigated storage medium and time conditions (p < 0.05). Modeling Liquid application yielded improved Ra values for the specimens stored in coffee at each time point (p < 0.05). Modeling Liquid application was associated with the lowest ΔE values in all investigated storage medium and time conditions (p < 0.05). Conclusion: Different types of modeling agents could affect the surface properties and discoloration of nano-hybrid composites.

• Journal of Information Technology Services
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• v.18 no.4
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• pp.67-82
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• 2019

The purpose of this study is to improve the calculation method of the software operation pricing proposed by the software business price calculation guide from 'input manpower method' to 'rate calculation method'. The software operation pricing of the input manpower method is not objectively calculated in the domestic IT outsourcing situation where the statistical data based on the activity based estimating is insufficient and it is decided by agreement between the owner and the client. In addition, there was no standard for adjusting the productivity according to the characteristics of the operation service. In order to improve this, an operational correction factor item that can affect the software operation productivity was selected based on foreign and domestic standards, and it was confirmed through the first questionnaire to IT operation managers. In order to determine the level of difficulty of the fixed operational correction factors, the operational correction factor using AHP technique was confirmed through a second questionnaire for pairwise comparison. The operational difficulty calculation table was developed with reference to COCOMO and ITIL standards. Finally, we propose a new pricing scheme that reflects the operating rate. Regression analysis was carried out by collecting the data of the domestic public institutions on the estimated cost and the actual cost calculated from the new rate method software operation pricing. The results of the regression analysis show that the estimated cost and the actual cost are related to each other. Mean magnitude of relative error(MMRE) and PRED[25] analysis were added for accuracy analysis. MMRE and PRED also showed satisfactory results, confirming the possibility of replacing the rate method software operation pricing.

• The KIPS Transactions:PartC
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• v.16C no.2
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• pp.183-188
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• 2009

Wireless sensor networks will be broadly deployed in the real world and widely utilized for various applications. A prerequisite for secure communication among the sensor nodes is that the nodes should share a session key to bootstrap their trust relationship. The open problems are how to verify the identity of communicating nodes and how to minimize any information about the keys disclosed to the other side during key agreement. At any rate, any one of the existing schemes cannot perfectly solve these problems due to some drawbacks. Accordingly, we propose a new pre-distribution scheme with the following merits. First, it supports authentication services. Second, each node can only find some indices of key spaces that are shared with the other side, without revealing unshared key information. Lastly, it substantially improves resilience of network against node capture. Performance and security analyses have proven that our scheme is suitable for sensor networks in terms of performance and security aspects.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.3
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• pp.223-231
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• 2009

This study intends to develop a GIS-based environmental assesment technique for environmental friendly road route plan using a spatial analysis function. After embodying an impact range based on the analysis of factors which cause the environmental damage by the road construction, a literature survey on a diverse grading cases and legal regulations was conducted. As a quantitative model for the spatial analysis, AHP was selected. As the environmental assessment, 8 items such as topography & geology, animals & plants, soil ecology, water quality, air quality, noise, land use, and landscape were selected. and then finally classified into three-grades using a pairwise comparison of the alate collected by a questionnaire surveying, weighting values were applied to there 8 items. The highest weight value, the grade 1 among eight was 0.191 for animals & plants. As the result of verifying the above method for the case region, the most environment-friendly road route could be generated.

• Mycobiology
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• v.48 no.5
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• pp.410-417
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• 2020

Fungal ecological interactions play a key role in structuring community assemblages. These associations may involve both antagonistic and synergistic relationships, which are commonly influenced by abiotic factors such as nutrient conditions. However, information for extreme, oligotrophic systems remain poor. Herein, interactions among key members of the aquatic transient fungal community (Aspergillus niger, Cladosporium sp., and Coprinellus micaceus) of a low-nutrient freshwater system in the Cuatro Ci enegas Basin, Mexico were studied. Pairwise interaction bioassays were explored in vitro under different nutrient conditions, including carbohydrates-rich, carbohydrates and amino peptides-rich, and low nutrients. Our results indicated that antagonistic patterns prevail among the studied taxa. However, nutrient-dependent changes were observed in Cladosporium sp. shifting to synergy under carbohydrates-rich conditions, suggesting changes in the fungal community composition as a result of nutrient enrichment. Remarkably, our findings contrast with previous work demonstrating mainly synergistic interactions between our tested fungal isolates and co-occurring autochthonous bacteria (Aeromonas spp. and Vibrio sp.) under low-nutrient conditions. This observation may indicate that bacteria and fungi exhibit distinct community-level responses, driven by nutrient conditions. This contributes to the knowledge of fungal community dynamics and interspecific interactions in an oligotrophic ecosystem, highlighting the relevance of nutrient-based shifts and antagonistic interactions in ecosystem dynamics.

• The Journal of Korean Physical Therapy
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• v.26 no.6
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• pp.449-452
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• 2014

Purpose: The purpose of this study was to analyze the coordination between trunk flexion and lower limb extension contributing to vertical propulsion during sit-to-stand (STS) at different chair heights in the elderly. Methods: Ten elderly subjects were asked to stand up at their natural speed from different chair heights : (1) $90^{\circ}$ knee flexion; (2) $100^{\circ}$ knee flexion; (3) $110^{\circ}$ knee flexion; and (4) $120^{\circ}$ knee flexion. A standard chair without a backrest or armrests was used in this study. To remove inertial effects of upper limb movements, subjects were asked to stand up from a chair with their arms crossed at the chest. Mean of results of three trials were used in the analysis at different knee flexion angles. Distances moved by the shoulder for compensatory trunk movement was recorded by motion analysis and vertical force was recorded under foot using force plates. Distances moved by the shoulder and vertical ground reaction force measurements were analyzed using repeated ANOVA. Results: Distances moved by the shoulder significantly decreased with higher chair (p<0.05). Vertical forces were not significant difference on chair heights (p>0.05), but results of pairwise comparisons for vertical force revealed significant difference between $90^{\circ}$ knee flexion and $120^{\circ}$ knee flexion (p<0.05). Conclusion: Trunk movement is probably used as a compensatory mechanism at low chair heights to increase lift-off from sitting by the elderly.

• Journal of Korean Society of Transportation
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• v.27 no.5
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• pp.63-74
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• 2009

One of the effective countermeasures for preventing traffic accidents is to provide traffic safety warning information to drivers. Provision of warning information would lead to safer driving to avoid accident occurrence. This study investigated the effects of in-vehicle warning information on driver's behavior. A variety of warning information contents using text, sound, and pictograms were prepared for the field experiments. Individual vehicle speed and acceleration data, which represent quantitative drivers' behavior in response to in-vehicle warning information, were collected using differential global positioning systems (DGPS). Statistical analyses including ANOVA and Tukey's pairwise comparison were conducted. It is expected that the results could be invaluable for designing more effective warning information.