• Herbal Formula Science
• /
• v.30 no.2
• /
• pp.37-44
• /
• 2022

Objectives : The purpose of this study was to examine the effects of Alisma canaliculatum Extract (ACE) on pacemaker potentials of small and large intestinal interstitial Cells of Cajal (ICC) in mice. Methods : We used enzymatic digestions to dissociate the ICC in the small and large intestine in mice. The whole-cell patch-clamp method was used to record pacemaker potentials in ICC. Results : 1. The ICC generated the pacemaker potentials in small intestine in mice. ACE (0.1-1mg/ml) induced membrane depolarization and decreased frequency with concentration-dependent manners. 2. Pretreatment with a Ca²⁺ free solution, Na⁺ 5 mM solution or 2-APB, a nonselective cation channel blocker, stopped the small intestinal ICC pacemaker potentials. In the case of Ca²⁺-free solution, Na⁺ 5 mM solution or 2-APB, ACE had no effects on the membrane depolarizations in small intestinal ICC. 3. The ICC generated the pacemaker potentials in large intestine in mice. Membrane depolarization appears regularly in the small intestine, but irregularly in the large intestine. ACE induced membrane depolarization (0.1-1mg/ml) and increased frequency (0.1-0.5mg/ml). 4. Pretreatment with a Ca²⁺ free solution, Na⁺ 5 mM solution or 2-APB, stopped the large intestinal ICC pacemaker potentials. In the case of Ca²⁺-free solution, Na⁺ 5 mM solution or 2-APB, ACE depolarized the membrane depolarizations in large intestinal ICC. 5. In mice, intestinal transit rate (ITR) values were dose-dependently decreased by the intragastric administration of ACE. Conclusions : These results suggest that ACE can regulate the pacemaker activity of ICC and the reaction by ACE is different from the small and large intestinal ICC, and the control of the intestinal motion by ACE may be caused by many complex processes.

• BMB Reports
• /
• v.49 no.11
• /
• pp.587-589
• /
• 2016

The circadian clock system enables organisms to anticipate the rhythmic environmental changes and to manifest behavior and physiology at advantageous times of the day. Transcriptional/translational feedback loop (TTFL) is the basic feature of the eukaryotic circadian clock and is based on the rhythmic association of circadian transcriptional activator and repressor. In Drosophila, repression of dCLOCK/CYCLE (dCLK/CYC) mediated transcription by PERIOD (PER) is critical for inducing circadian rhythms of gene expression. Pacemaker neurons in the brain control specific circadian behaviors upon environmental timing cues such as light and temperature cycle. We show that amino acids 657-707 of dCLK are important for the transcriptional activation and the association with PER both in vitro and in vivo. Flies expressing dCLK lacking AA657-707 in $Clk^{out}$ genetic background, homologous to the mouse Clock allele where exon 19 region is deleted, display pacemaker-neuron-dependent perturbation of the molecular clockwork. The molecular rhythms in light-cycle-sensitive pacemaker neurons such as ventral lateral neurons ($LN_vs$) were significantly disrupted, but those in temperature-cycle-sensitive pacemaker neurons such as dorsal neurons (DNs) were robust. Our results suggest that the dCLK-controlled TTFL diversify in a pacemaker-neuron-dependent manner which may contribute to specific functions such as different sensitivities to entraining cues.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.27 no.1
• /
• pp.112-117
• /
• 2013

We studied the modulation of pacemaker activities by Samchulkunbi-tang (SCKB) in cultured interstitial cells of Cajal (ICC) from murine small intestine with the whole-cell patch-clamp technique. Externally applied SCKB produced membrane depolarization in the current-clamp mode. The pretreatment with $Ca^{2+}$-free solution and thapsigargin, a $Ca^{2+}$-ATPase inhibitor in endoplasmic reticulum, abolished the generation of pacemaker potentials and suppressed the SCKB-induced action. The application of flufenamic acid (a nonselective cation channel blocker) abolished the generation of pacemaker potentials by SCKB. However, the application of niflumic acid (a chloride channel blocker) did not inhibit the generation of pacemaker potentials by SCKB. In addition, the membrane depolarizations were inhibited by not only GDP-${\beta}$-S, which permanently binds G-binding proteins, but also U-73122, an active phospholipase C inhibitor. These results suggest that SCKB modulates the pacemaker activities by nonselective cation channels and external $Ca^{2+}$ influx and internal $Ca^{2+}$ release via G-protein and phospholipase C-dependent mechanism. Therefore, the ICC are targets for SCKB and their interaction can affect intestinal motility.

• Journal of Chest Surgery
• /
• v.42 no.2
• /
• pp.256-258
• /
• 2009

A 63-year-old man who had a permanent pacemaker placed 17 years ago presented to us with local inflammatory signs on his chest. The skin was opened to remove the permanent pacemaker. However, the pacing wire was not easily removed. So, the permanent pacemaker was removed from the chest with leaving the remnant wire inside. However, the local infection recurred because of the remnant wire. Right thoracotomy was performed and cardiopulmonary bypass was started. After total circulatory arrest, the right atrium was opened and the wire was completely removed.

• Korean Journal of Adult Nursing
• /
• v.17 no.3
• /
• pp.484-492
• /
• 2005

Purpose: This study was undertaken to identify the effect of providing information on anxiety, knowledge and compliance in permanent pacemaker patients. Method: A quasi experimental design with non-equivalent control group and non-synchronized design was used. The subjects of this study were 50 patients who had received permanent pacemaker implantation at a university hospital in Seoul. They were divided into an experimental group of 22 patients who received education and a control group of 28 patients. The education was composed of group education(twice) and individualized reinforcement education(once) using an education booklet. Results: Anxiety decreased in the experimental group. Knowledge significantly increased in the experimental group compared to that in the control group. Compliance significantly increased in the experimental group. Conclusion: It can be concluded that providing information is effective for reducing anxiety, increasing knowledge and improving compliance of the permanent pacemaker patients.

• Journal of Korean Critical Care Nursing
• /
• v.11 no.3
• /
• pp.23-34
• /
• 2018

Purpose : This study develops a substantive theory on the recipients' experience of adapting to a permanent pacemaker. Method : The study used the grounded theory method developed by Strauss and Corbin (1998). The participants of the study were 13 adults who had received a permanent pacemaker during the previous year. The study addressed the research question "what is the experience of adaptation in people who had received permanent pacemakers?" From October 2016 to March 2017, data were collected from the participants through in-depth interviews. Results : The core category indicating the essence of the adaptation experience was shown to be "accepting the pacemaker as part of my body and living in line with it." Conclusions : In nursing practice, the results of this study will assist nurses in improving their communications with and developing guidelines or interventions for their clients who have received permanent pacemakers. In the field of nursing education, this study is expected to provide a framework to understand the experiences of future nurses and other healthcare workers working with permanent pacemaker recipients.

• Journal of Chest Surgery
• /
• v.32 no.7
• /
• pp.668-670
• /
• 1999

The most important indication for the removal of the lead-electrode system is the presence of an infection. When an infection occurs, the entire pacemaker system including the impluse generator and lead-electrode system should be removed. The entrapped electrode can be removed by a continous traction, by the use of forceps, snares or baskets, by the use of a locking stylet or a dilator sheath, and by an operation. We report a case that underwent a removal of an entrapped transvenous pacemaker electrode by the use of snaring technique.

• Journal of Chest Surgery
• /
• v.14 no.2
• /
• pp.168-174
• /
• 1981

Since cardiac pacemaker was first totally implanted by Chardack, Gage and Greatbatch [1966], the electrical circuity of the cardiac pacemaker has been improved, modified, and refined. The problem of transvenous electrodes, however, is still remained; this may be due to electrode displacement, exit and/or entrance block, lead fracture and insulation defects. In permanent cardiac pacing, Irreversible loss of function of the transvenous electrode catheter eventually requires insertion of new lead. Authors now report one case that disclosed easy displacement of electrode tip in early phase of implantation and then two years and five months later, malfunctioning electrode could not be withdrawn from the cardiovascular system because it has become firmly enclosed by fibrous tissue along its course from the vein tract to the right ventricle. Under such circumstances, the electrode catheter tip was left in tricuspid annulus after being sutured at its entrance and burying the loop of lead in generator pocket. New other one electrode was then reimplantation through left external jugular vein.

• Journal of Chest Surgery
• /
• v.53 no.2
• /
• pp.82-85
• /
• 2020

Insertion of an epicardial pacemaker is a useful treatment for pediatric patients with an abnormal heart rhythm. However, there are limitations and concerns when implanting epicardial pacemakers in infants and neonates due to their small body size. We report a patient who experienced rare complications after implantation of a permanent pacemaker.

• Journal of electromagnetic engineering and science
• /
• v.10 no.4
• /
• pp.206-211
• /
• 2010

In this paper, an implantable planar inverted-F antenna (PIFA) for an artificial cardiac pacemaker is proposed. The antenna has a simple structure with a low profile and is placed on the top side of the pacemaker. The dimensions of the pacemaker system, including the antenna element, are $42{\times}43.6{\times}11$ mm. When the antenna is embedded in pig tissue, its $S_{11}$ value is -10.94 dB at 403 MHz and the -10 dB impedance bandwidth of the antenna is 6 MHz (399~406 MHz). The proposed PIFA in tissue has a peak gain of -20.19 dBi and a radiation efficiency of 1.12 % at 403 MHz. When the proposed antenna is placed in a flat phantom, its specific absorption ratio (SAR) value is 0.038 W/kg (1 g tissue). Performances of the proposed PIFA is sufficient to operate at the MICS band (402 ~ 405 MHz).