• Title/Summary/Keyword: pH-sensitivity

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Fabrication and Electrical Properties of Anodic Aluminum Oxide Membrane with Various Anodizing Temperatures for Biosensor (바이오센서로 응용을 위한 양극산화알루미늄의 양극산화 온도에 따른 제작 및 전기적 특성)

  • Yeo, Jin-Ho;Lee, Sung-Gap;Kim, Yong-Jun;Lee, Young-Hee
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.27 no.6
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    • pp.394-398
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    • 2014
  • We fabricated the electrolyte-dielectric-metal (EDM) sensor on the base of AAO (anodic aluminum oxide) template with variation of the anodizing temperature. When a surface is immersed or created in an aqueous solution, a discontinuity is formed at the interface where such physicochemical variables as electrical potential and electrolyte concentration change significantly from the aqueous phase to another phase. Because of the different chemical potentials between the two phases, charge separation often occurs at the interfacial region [1]. This interfacial region, togeter with the charged surface, is usually known as the electrical double layer (EDL) [2]. The structural and electrochemical properties of AAO sensor were investigated for applications in capacitive pH sensors. To change the thickness of the AAO template, the anodizing temperature was varied from $5^{\circ}C$ to $20^{\circ}C$, the thickness of the AAO template invreased from 300 nm to 477 nm. The pH sensitivity of sensors with the anodizing temperature of $20^{\circ}C$ showed the highest value of 56.4 mV/pH in the pH range of 3 to 11. The EDM sensor with the anodizing temperature of $20^{\circ}C$ exhibited the best long-term stability of 0.037 mV/h.

An Advanced Kinetic Method for HO2·/O2-· Determination by Using Terephthalate in the Aqueous Solution

  • Kwon, Bum Gun;Kim, Jong-Oh;Kwon, Joong-Keun
    • Environmental Engineering Research
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    • v.17 no.4
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    • pp.205-210
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    • 2012
  • Hydroperoxyl radical/superoxide anion radical ($HO_2{\cdot}/O_2^-{\cdot}$, $pK_a$=4.8) as an intermediate is of considerable importance in oxidation processes. Hence, the method of detecting $HO_2{\cdot}/O_2^-{\cdot}$ with high sensitivity is necessary to be developed. To achieve this objective, this study newly employed terephthalate (TA) as a probe for the measurement of $HO_2{\cdot}/O_2^-{\cdot}$ in the kinetic method presented in our previous study. This method was based on the hydroxylation of TA to produce mainly hydroxyterephthalic acid or hydroxyterephthalate (OHTA), which was analyzed by fluorescence detection (${\lambda}_{ex}$=315nm, ${\lambda}_{ex}$=425nm). The life-time of $HO_2{\cdot}/O_2^-{\cdot}$ and its concentration formed from the photolysis technique of $H_2O_2$ were reported in this study. At range of pH 2-10, the life-time of $HO_2{\cdot}/O_2^-{\cdot}$ was 51-422 sec. In particular, an increase in the life-time with pH was observed. The sensitivities of the kinetic method by using TA were always higher with 1.7-2.5 times at pH 8.0 than those by using benzoic acid. From these results, this study can contribute to understanding the basic functions of $HO_2{\cdot}/O_2^-{\cdot}$ in oxidation processes.

Characterization of a cysteine proteinase from adult worms of Paragonimus westermani (폐흡충(Parnonimr westemani)성충에서 정제한 cysteine proteinase의 특성)

  • 송철용;김동수
    • Parasites, Hosts and Diseases
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    • v.32 no.4
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    • pp.231-242
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    • 1994
  • Pnragonimus westermnni, the lung fluke, is known to migrate to the pulmonary tissue of mammalian hosts and causes pathological changes in the lungs. An acidic thiol-dependent proteinase with a molecular weight of approximately 20,000 daltons was purified to homogeneity using ion-exchange chromatography and gel filtration chromatography. On SDS-PAGE, the molecular weight of the enzyme was 17,500 daltons. Isoelectric point was 6.45. The enzyme was similar to the acidic cysteine proteinase of vertebrates in the properties of pH optimum, substrate specificity and inhibitor sensitivity. Enzymatic activity was stable at pH 5.5 for at least two days when stored at 4℃. The cysteine proteinase was capable of degrading collagen and hemoglobin. Sera of patients with paragonimiasis and mice infected with R westermani reacted in immunoblots with the partially purified proteinase. This result suggested that the cysteine proteinase of P. westermnni may play a role in migration in tissues, and in acquisition of nutrients by parasites from the host. It is also potentially an antigen for the serodiagnosis of paragonimiasis.

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TCE, PCE 측정을 위한 바이오센서의 특성

  • 류두현;김용미;최상일
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.388-391
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    • 2004
  • A sol-gel fiber-optic biosensor with encapsulated pH-sensitive fluorophore and immobilized genetically modified toluene-o-xylene monooxygenase was developed to detect TCE and PCE, which are carcinogenic chlorinate organic compounds prevailing in ground water. The sensitivity was characterized for the composition of sol-gel, and manufacturing procedure. The intensity curve reveals a linear range of intensity for pollutant concentration range of 0.01ppm and 1ppm. The change in intensity was appeared to be larger at each of L for same condition, and, therefore, the wavelength of λ was chosen for the analytical measurement.

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Development of New Fibers Related Sensitivity and Comfortability -Preparation of Polystyrene microcapsules containing Aqueous material and their Application- (감성기능 섬유신소재의 개발(II) -수용성 기능성물질 함유 폴리스티렌 마이크로캡슐의 제조와 섬유에의 응용-)

  • Hong, Ki Jeong;Park, Soo Min
    • Textile Coloration and Finishing
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    • v.9 no.3
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    • pp.33-41
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    • 1997
  • Polystyrene microcapsules were prepared by microencapsulation technique of solvent-evaporation. These microcapsules were characterized by Infrared spectroscopy, thermal analysis, scanning electron microscopy, and shape analysis. Fragrant water was used as a core material. Thermal and pH stabilities of polystyrene microcapsules were relatively excellent. And functional finishing on cotton fabric with microcapsules was applied.

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Shuffled toluene-o-xylene monooxygenase를 이용한 TCE 측정용 fluorescence biosensor

  • ;;;;Thomas Wood
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.475-478
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    • 2003
  • A sol-gel fiber-optic biosensor with encapsulated pH-sensitive fluorophore and immobilized genetically modified toluene-o-xylene monooxygenase was developed to detect TCE, which is carcinogenic chlorinate organic compounds prevailing in ground water. The sensitivity was characterized for the composition of sol-gel, and manufacturing procedure.

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Growth Responses of Lettuce Seeds Germinated in Aqueous Solutions of Sulfur Dioxide (아황산 수용액에서 발아시킨 상치 종자의 생장반응)

  • 이미순
    • Journal of Plant Biology
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    • v.18 no.4
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    • pp.155-160
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    • 1975
  • Growth responses of lettuce seeds germinated in aqueous solutions of $SO_2$ were investigated for mode of action studies of this gas. The concentration and pH of $SO_2$ solution greatly affected the growth of germinating seeds. Root growth was more sensitive to $SO_2$ than hypocotyl growth. The sensitivity of total seedling growth lay between root and hypocotyl growth. Growth inhibition of germinating seeds appeared more serioius when $SO_2$ was added during the early stage of germination.

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Spectroscopic Studies of Eu(III) Complexes with Iminodiacetic and Methyliminodiacetic Acids

  • 김동철;윤수경;김윤두;강준길;배준현;김종구
    • Bulletin of the Korean Chemical Society
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    • v.19 no.1
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    • pp.83-86
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    • 1998
  • The luminescence spectra of Eu(Ⅲ) complexes have been measured as a function of molar metal-to-ligand ratio and pH. The ligands used in this study are tridentate iminodiacetate (IDA) and methyliminodiacetate (MIDA). The 620 nm emission band, attributed to the $^5D_0{\to}^7F_2$ transition, is monitored to investigate the optimum condition for $EuL_3^{3-}$ complex formation in aqueous state. In addition, the sensitivity of the 465 nm absorption band, attributed to the $^7F_0{\to}^5D_2$ transition, to the ligand environment has been also investigated.

Characteristics and pathogenicity of Cladobotryum mycophilum isolated from cobweb disease of button mushroom (Agaricus bisporus) in Korea

  • Lee, Chan-Jung;Han, Hye-Su;Jhune, Chang-Sung;Cheong, Jong-Chun;Oh, Jin-A;Kong, Won-Sik
    • Journal of Mushroom
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    • v.9 no.4
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    • pp.198-201
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    • 2011
  • Cobweb disease symptoms were observed in a mushroom farm in Buye, Korea during a disease survey in 2008-2011. Five isolates of Cladobotryum sp. were obtained from the infected caps and stipes. These isolates of Cladobotryum sp. were identified as C. mycophilum based on their morphological, cultural characteristics and analysis of the ITS sequences. Early symptoms were noticed as round, fleshy, yellowish brown lesions on mushroom caps. Late symptoms progressed when the parasitic fungus formed white cobweb circular colonies on dead or damaged pinheads, spread on the surface of the casing, and covered entirely fruiting bodies. Optimal temperature and pH for mycelial growth on MEA is $23^{\circ}C$ and 6.0. Microscopically the spores of the fungus are large and most 2~3 celled produced on vertically branched conidiophores. Mushroom caps turned dark brown and shrunk due to soft rot. Testing of sensitivity to selected fungicides showed that isolate was highly resistance to Mancozeb and Thiophanate-methyl, moderately sensitivity to Iprodione, and highly sensitivity to Benomyl, Prochloraz-Mn and Carbendazim.

Studies on the Edwardsiellosis of cultured flounder, Paralichthys oliuaceus;- Characteristics, pathgenicity and control of Edwardsiella tarda - (양식넙치(Paralichthys olivaceus)의 복수증에 관한 연구 - Edwardsiella tarde의 일부 특성과 병원성 및 대책 -)

  • 한재철;김영진;서형석;김영길;이근광;안병목
    • Korean Journal of Veterinary Service
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    • v.20 no.1
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    • pp.11-18
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    • 1997
  • Characteristics, pathogenicity and control of the causative organisms isolated front diseased cultured flounder, Paralichthys olivaceus were studied. The causative organisms were identified as E tarda by biochemical and biophysical characteristics. Also, it strains were named as E tarda KBF-1 and E tarda KMF-1, and optimal pH of E tarda KBF-1 and E tarda KMF-1 were 8.0, and optimal concentration of NaCl. E tarda KBF-1 was 0% and E tarda KMF-1 was 1%. In the pathogenicity test, 0~10 of the flounders of artificially infected group(E tarda KBF-1) with $1.0{\times}10^7$ cfu/fish were died within 60 hrs, but 0~9 flounders infected group with 41.0{\times}10^6$ cfu/fish were died within 60 hrs. Also, 0~10 flounders infected group(E tarda KMF-1) with $1.0{\times}10^7$ cfu/fish were died within 36 hrs, while 0~7 flounders infected with $1.0{\times}10^6$ cfu/fish were died within 60 hrs. Drug sensitivity of E tarda KBF-1 strain was resistant to AM, CF and N, and intermediate to E, K and S, and sensitivity to C, G, SxT and FF. But E tarda KMF-1 strain was resistant to CF, E and V, and intermediate to AM, C, N and SxT, and sensitivity to GM and FF.

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