• Title/Summary/Keyword: pH-mapping

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Molecular Characterization, Chromosomal Localizations, Expression Profile, and Association Analysis of the Porcine PECI Gene with Carcass Traits

  • Gao, H.;Fan, B.;Zhu, M.J.;Liu, Bang
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.1
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    • pp.7-12
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    • 2010
  • The full-length cDNA of the porcine peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase (PECI) gene encodes a monofunctional peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase. Cloning and sequencing of the porcine PECI cDNA revealed the presence of an 1185-base pair open reading frame predicted to encode a 394-amino acid protein by the 5'rapid amplification of cDNA ends (5'RACE) and EST sequences. The porcine PECI gene was expressed in seven tissues (heart, liver, spleen, lung, kidney, skeletal muscle, fat) which was revealed by reverse transcriptase-polymerase chain reaction (RT-PCR). The porcine PECI was mapped to SSC71/2 p11-13 using the somatic cell hybrid panel (SCHP) and the radiation hybrid panel (RH) (LOD score 12.84). The data showed that PECI was closely linked to marker S0383. A C/T single nucleotide polymorphism in PECI exon 10 (3'UTR) was detected as a PvuII PCR-RFLP. Association analysis in our experimental pig population showed that different genotypes of PECI gene were significantly associated with the Average Backfat thickness (ABF) (p<0.05) and Buttock backfat thickness (p<0.01).

On Learning and Structure of Cerebellum Model Linear Associator Network(I) -Analysis & Development of Learning Algorithm- (소뇌모델 선형조합 신경망의 구조 및 학습기능 연구(I) -분석 및 학습 알고리즘 개발-)

  • Hwang, H.;Baek, P.K.
    • Journal of Biosystems Engineering
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    • v.15 no.3
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    • pp.186-198
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    • 1990
  • 인간 소뇌의 구조와 기능을 간략하게 수학적으로 모델링하여 입력에 따른 시스템의 적정 출력을 학습에 의한 적응 제어 방식으로 추출해 내는 소뇌모델 대수제어기(CMAC : Cerebellar Model Arithmetic Controller)가 제안되었다. 본 논문에서는 연구개발된 기존 신경회로망과의 비교 분석에 의거하여, 소뇌모델 대수제어기 대신 네트의 특성에 따라 소뇌모델 선형조합 신경망(CMLAN : Cerebellum Model Linear Associator Network)이라 하였다. 소뇌모델 선형조합 신경망은 시스템의 제어 함수치를 결정하는 데 있어, 기존의 제어방식이 시스템의 모델링을 기초로 하여 알고리즘에 의한 수치해석적 또는 분석적 기법으로 모델 해를 산출하는 것과 달리, 학습을 통하여 저장되는 분산기억 소자들의 함수치를 선형적으로 조합함으로써 시스템의 입출력을 결정한다. 분산기억 소자로의 함수치 산정 및 저장은 소뇌모델 선형조합 신경망이 갖는 고유의 구조적 상태공간 매핑(State Space Mapping)과 델타규칙(Delta Rule)에 의거한 시스템의 입출력 상태함수의 학습으로써 수행된다. 본 논문을 통하여 소뇌모델 선형조합신경망의 구조적 특성, 학습 성질과 상태공간 설정 및 시스템의 수렴성을 규명하였다. 또한 기존의 최대 편차수정 학습 알고리즘이 갖는 비능률성 및 적용 제한성을 극복한 효율적 학습 알고리즘들을 제시하였다. 언급한 신경망의 특성 및 제안된 학습 알고리즘들의 능률성을 다양한 학습이득(Learning Gain)하에서 비선형 함수를 컴퓨터로 모의 시험하여 예시하였다.

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RENARKS ON REWEAKLY COMMUTING MAPPONGS AND COMMON FIXED POINT THEOREMS

  • Pathak, H.-K;Cho, Y.-J;Kang, S.-M
    • Bulletin of the Korean Mathematical Society
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    • v.34 no.2
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    • pp.247-257
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    • 1997
  • It was the turning point in the "fixed point arena" when the notion of weak commutativity was introduced by Sessa [9] as a sharper tool to obtain common fixed points of mappings. As a result, all the results on fixed point theorems for commuting mappings were easily transformed in the setting of the new notion of weak commutativity of mappings. It gives a new impetus to the studying of common fixed points of mappings satisfying some contractive type conditions and a number of interesting results have been found by various authors. A bulk of results were produced and it was the centre of vigorous research activity in "Fixed Point Theory and its Application in various other Branches of Mathematical Sciences" in last two decades. A major break through was done by Jungck [3] when he proclaimed the new notion what he called "compatibility" of mapping and its usefulness for obtaining common fixed points of mappings was shown by him. There-after a flood of common fixed point theorems was produced by various researchers by using the improved notion of compatibility of mappings. of compatibility of mappings.

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Purification and Characterzation of a Restriction Endonuclease from Pseudomonas syringae pv.phaselicola (Pseudomonas syringe pv. phaseolicola로 부터 제한효소의 분리정제 및 특성)

  • Bae, Moo;Lee, Eun-Young
    • Microbiology and Biotechnology Letters
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    • v.22 no.5
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    • pp.485-490
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    • 1994
  • A restriction endonuclease, PsyI, has been isolated from Pseudomonas syringae pv. pha- seolicola, and its catalytic properties have been studied. This enzyme was purified through strepto- mycin sulfate and ammonium sulfate fractionation, phosphocellulose Pll, DEAE-cellulose, hydroxy- apatite and Sephadex G-100 column chromatography. It's molecular weight was about 50,000 dalton as determined by 7.5% polyacrylamide gel electrophoresis containing 0.1% SDS. In catalytic proper- ties, PsyI shows stable at wide ranges of pH between 7.0 and 10.0, of temperature between 30$\circ$C and 37$\circ$C, and its thermal stability is between 25$\circ$C, and 45$\circ$C, at the presence Of 10 mM MgCl$_{2}$-PsyI essentially require Na salt for enzyme reaction, is rather inhibited in the high Na salt concent- ration. The presence of 2-mercaptoethanol is absolutely required for the enzyme activity. This endonuclease, PsyI was determined to be an isoschizomer of SalI from the results of the restriction mapping and DNA sequencing.

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Karyotypic Analysis and Physical Mapping of rRNA Gene Loci in Persicaria tinctoria (쪽의 핵형분석과 rRNA 유전자의 염색체상 위치)

  • Choi, Hae-Woon;Lee, Sang-Hoon;Kim, Soo-Young;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.3
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    • pp.195-198
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    • 2008
  • Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 ${\mu}m$ to 1.50 ${\mu}m$ in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1,2,3,4,6,7,8,9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.

Re-writing World Literature through Juxtaposition: Decolonizing Comparative Literature in Vietnam

  • Pham, Chi P.;Do, Ninh H.
    • SUVANNABHUMI
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    • v.14 no.1
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    • pp.9-29
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    • 2022
  • Postcolonial critics have criticized Comparative Literature for exclusively studying literatures from the non-Western world through Western lenses. In other words, postcolonial criticism asserts that theorists and practitioners of comparative literature have traced the "assistance" of the classic "comparison and contrast" approach to an imperialist discourse, which sustains the superiority of Western cultures and economies. As a countermeasure to reading through the comparative lens, literary theories have offered a "juxtapositional model of comparison" that connects texts across cultures, places, and times. This paper examines practices of Comparative Literature in Vietnam, revealing how the engagement with decolonizing processes leads to a knowledge production that is paradoxically colonial. The paper also analyses implementations of this model in reading select Vietnamese works and highlights how conventional comparisons, largely based on historical influences and reception, maintain the colonial mapping of World Literature, centralizing Western, and more particularly, English Literature and in the process marginalizing the others. Therefore, the practice of juxtaposing Vietnamese literary works with canonical works of the World Literature will provoke dialogues and raise awareness of hitherto marginalized works to an international readership. In this process, the paper considers the contemporary interest of Comparative Literature practice in trans- national, trans-regional, trans-historical, and trans-cultural perspectives.

Characterization and Mapping of the Bovine FBP1 Gene

  • Guo, H.;Liu, W-S.;Takasuga, A.;Eyer, K.;Landrito, E.;Xu, Shang-zhong;Gao, X.;Ren, H-Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.9
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    • pp.1319-1326
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    • 2007
  • Fructose-1,6-bisphosphatase (FBP1) is a key regulatory enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose-1,6-bisphosphate to generate fructose-6-phosphate and inorganic phosphate. Deficiency of fructose-1, 6-bisphosphatase is associated with fasting hypoglycemia and metabolic acidosis. The enzyme has been shown to occur in bacteria, fungi, plants and animals. The bovine FBP1 gene was cloned and characterized in this study. The full length (1,241 bp) FBP1 mRNA contained an open reading frame (ORF) encoding a protein of 338 amino acids, a 63 bp 5' untranslated region (UTR) and a 131 bp 3' UTR. The bovine FBP1 gene was 89%, 85%, 82%, 82% and 74% identical to the orthologs of pig, human, mouse, rat and zebra fish at mRNA level, and 97%, 96%, 94%, 93% and 91% identical at the protein level, respectively. This gene was broadly expressed in cattle with the highest level in testis, and the lowest level in heart. An intronic single nucleotide polymorphism (SNP) (A/G) was identified in the $5^{th}$ intron of the bovine FBP1 gene. Genotyping of 133 animals from four beef breeds revealed that the average frequency for allele A (A-base) was 0.7897 (0.7069-0.9107), while 0.2103 (0.0893-0.2931) for allele B (G-base). Our preliminary association study indicated that this SNP is significantly associated with traits of Average Daily Feed Intake (ADFI) and Carcass Length (CL) (p<0.01). In addition, the FBP1 gene was assigned on BTA8 by a hybrid radiation (RH) mapping method.

Antigenic Determinant Mapping in preS2 Region of Hepatitis B Surface Antigen (B형 간염바이러스 표면항원 preS2 부위의 항원결정인자 규명)

  • 권기선;김창수;박주상;한문희;유명희
    • Korean Journal of Microbiology
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    • v.28 no.1
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    • pp.13-18
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    • 1990
  • A DNA sequence encoding the adr subtype preS2 region of hepatitis B virus envelope protein was fused to 5' end of lacZ gene yielding a plasmid pTSZ, in order to produce a preS2-$\beta$-galactosidase fusion protein. Serial deletions from 3' and 5' end of preS2 were constructed in plasmids, which were expressed and their antigenicities were examined with the monoclonal antibody H8. Deletions from amino and carboxy terminal to certain points did not affect the antigenicity, but the longer deletions destroyed the antigenicity. End points of deleted preS2 sequence were determined by DNA sequencing. As a result, each end of preS2 epitope was located in the region of amino acid residue 130-132 and 140-142, respectively. Residue 143 may be supplementary for antigenic epitope since the deletion from carboxy terminal to residue 143 revealed partial defect of antigenicity. In the interval of antigenic epitope the amino acid differences between adr and adw2 subtype occurred ar residue 130, 132, and 141. This result indicated that one or more of the three residues are responsible for the binding specificity of monoclonal antibody H8 to adr subtype preS2 fusion protein.

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Variation of Soil Characteristics in Lava Plain (용암류대지(熔岩類臺地)에 분포(分布)한 토양(土壤) 특성(特性)의 변이분석(變異分析))

  • Park, Chang-Seo;Um, Ki-Tae;Min, Kyeong-Beom
    • Korean Journal of Soil Science and Fertilizer
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    • v.17 no.4
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    • pp.343-348
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    • 1984
  • Variation of soil properties within mapping unit of lava plain soils was statistically summarized. Properties such as particle size distribution, moisture retention, color, pH, and CEC are relatively unaffected by soil management while Na, K, base saturation, and available $P_2O_5$ most affected by management. These soils were correctly classified with regard to order at 66.5, to greatgroup at 56.0, and to series at 43.8%. CV values greater than 90% could be symptomatic of skew distribution. Distributions of sand content and some chemical properties were log-normal.

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Sex Differences in Cerebellar Structure of Healthy Adults (정상 성인에서 남녀의 소뇌 구조 차이)

  • Kim, Ji-Hyun H.;Bae, Su-Jin;Ryu, Keun-Taik;Kang, Min-Seong;Lim, Soo-Mee;Lee, Sun-Ho;Lee, So-Jin;Ko, Eun;Jeong, Do-Un
    • Korean Journal of Biological Psychiatry
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    • v.19 no.2
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    • pp.77-83
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    • 2012
  • Objectives : Although there have been studies that examine sex differences of the brain structures using magnetic resonance imaging, studies that specifically investigate cerebellar structural differences between men and women are scarce. The purpose of current study was to examine sex differences in structures of the cerebellum using cerebellar template and cerebellum analysis methods. Methods : Sixteen men and twenty women were included in the study. A MATLAB based program (MathWorks, Natick, MA, USA), Statistical Parametric Mapping 5 (SPM5) using the spatially unbiased infra-tentorial atlas template (SUIT) as the cerebellum template, was used to analyze the brain imaging data. Results : There was no significant difference in age between men (mean age=28.1) and women (mean age=27.2). Men showed higher gray matter density than women in two left cerebellar areas including the clusters in the lobules IV and V (a cluster located across the lobules IV and V), and the lobule VIIIb (lobules IV and V, t=4.75, p<0.001 ; lobule VIIIb, t=3.08, p=0.004). Conclusions : The current study found differences in cerebellar gray matter density between men and women. The current study holds its significance for applying the template specifically developed for the analysis of cerebellum.