• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.195-195
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• 2012

Recently, graphene based solution-gated field-effect transistors (SGFETs) have been received a great attention in biochemical sensing applications. Graphene and reduced graphene oxide (RGO) possess various advantages such as high sensitivity, low detection limit, label-free electrical detection, and ease of fabrication due to their 2D nature and large sensing area compared to 1D nanomaterials- based nanobiosensors. Therefore, graphene or RGO -based SGFET is a good potential candidate for sensitive detection of protons (H+ ions) which can be applied as the transducer in various enzymatic or cell-based biosensing applications. However, reports on detection of H+ ions using graphene or RGO based SGFETs have been still limited. According to recent reports, clean graphene grown by CVD or exfoliation is electrochemically insensitive to changes of H+ concentration in solution because its surface does not have terminal functional groups that can sense the chemical potential change induced by varying surface charges of H+ on CVD graphene surface. In this work, we used RGO -SGFETs having oxygen-containing functional groups such as hydroxyl (OH) groups that effectively interact with H+ ions for expectation of increasing pH sensitivity. Additionally, we also investigate RGO based SGFETs for bio-sensing applications. Hydroloytic enzymes were introduced for sensing of biomolecular interaction on the surface of RGO -SGFET in which enzyme and substrate are acetylcholinesterase (AchE) and acetylcholine (Ach), respectively. The increase in H+ generated through enzymatic reaction of hydrolysis of Ach by AchE immobilized on RGO channel in SGFET could be monitored by the change in the drain-source current (Ids).

• BMB Reports
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• v.35 no.4
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• pp.384-388
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• 2002

A method for the ratiometric pH sensing using LysoSensor DND-192 is presented in this paper. It works in the physiological pH range. It is based on the use of two fluorophores which differ significantly in their lifetimes. As the discrimination of their emissions is performed through two different frequencies, this method can allow significant overlap of the emission spectra. A simple long-pass filter, or a combination of long-and short-pass filters, was used instead of narrow-bandpass devices. Importantly, the measurements were carried out under strong ambient light. The method could be used in a wide variety of applications, such as intracellular measurements, microscopy, bioprocess monitoring, etc.

• Journal of the Optical Society of Korea
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• v.10 no.1
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• pp.28-32
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• 2006

We propose a new pH-sensing scheme using a methylene blue adsorption on an optical fiber cladding surface. Interactions between the silica and hydroxyl ions of a base solution induce the surface of the silica negatively charged. The charged surface attracts the positively charged chromophores of methylene blue. As the pH of the solution is reduced, the electrostatic attraction will also be reduced. This electrostatic attraction can change the transmitted light intensity of the cladding mode, since the boundary condition changes. We also carried out a simulation to verify the effect from external refractive index change around a long-period fiber grating. Our results confirm that the wavelength shift by external refractive index change is negligible compared to the transmitted light intensity variation of the cladding mode. By using a long-period grating pair, we can detect the cladding mode transmittance variations. Experimentally, we showed the possibility of pH sensing in the $1.5{\mu}m$ infrared region.

• Proceedings of the KSRS Conference
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• 2004.10a
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• pp.61-63
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• 2004

The procurement of a mobile microwave scatterometer platform involved the consideration to ensure a mobile platform and equipment selected full-filled technical requirement and safety standard in Malaysia. Designing, and modification works involved engineering methodology in determining and selecting a suitable hydraulic telescopic boom that suit a selected mobile platform available locally. The mobile platform is a delivery system for microwave remote sensing microwave scatterometer and other accessories to any locations in Malaysia. Total loading to be carried by the mobile platform is 4500 kg and its overall weight must be 16,000 kg as recommended by hydraulic telescopic boom manufacturers. The telescopic boom will elevate microwave scatterometer system including the antenna to a maximum height of 27 m, and can also be rotated through $3600^{\circ}$. A mechanism is incorporated in the system to enable tracking or monitoring angular movement of the hydraulic telescopic boom when positioned towards predetermined target.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.1
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• pp.89-93
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• 2009

In this paper, we investigated effect of sensing time on the performance of enhanced spectrum sensing method, which is the energy detector with multiples of verification using time delay, under Suzuki channel. We assumed that SNR is 1dB, $P_{FA}=0.1$ and various mobile speed such as 3, 60, 110 km/h. The performance is investigated by simulation and compared to that of conventional energy detector.

• Bulletin of the Korean Chemical Society
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• v.25 no.7
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• pp.997-1002
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• 2004

A hydrogenperoxide sensor containing peroxidase extracted from horseradish was constructed and pH effect on its sensing ability was investigated. Current profiles of the biosensor with pH and the electrophoretic analysis showed that horseradish peroxidase consists of two isozymes. Assuming that it is a hypothetical twoisozyme mixture, the current profiles were deconvoluted into two Gaussians. Application of the new Michaelis-Menten equation connoting pH concept to this system enabled to find all the related dissociation constants of the isozyme-substrates and the isozyme-proton complexes and to determine pHs for the maximal isozyme activities.

• Proceedings of the KIEE Conference
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• 1988.07a
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• pp.301-304
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• 1988

A pH-meter has been newly developed by using on ISFET as the ion sensing element. The performance characteristics of the developed pH-meter have been investigated and confirmed to be used practically. The stable operation and the temperature compensation were stressed in this study.

• Proceedings of the KIEE Conference
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• 1987.07a
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• pp.493-496
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• 1987

Theoretical analysis was performed to understand the sensing mechanism of pH-ISFET by using the site binding model. The calculated pH-${\varphi}$ relationship showed good fittings with the experimental results.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.1009-1011
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• 2003

The procurement of mobile microwave scatterometer involve the consideration to ensure vehicle and equipment selection full-filled technical requirement and safety standard in Malaysia. Designing, and modification works involve engineering methodology in determining and selecting a suitable hydraulic telescopic boom that suit a selected vehicle available from the market. The vehicle is also a delivery system for microwave remote sensing equipment and other accessories to any locations in Malaysia. Total loading to be carried by the vehicle is about 4500 kg and its overall weight must be 16,000 kg as recommended by hydraulic telescopic boom manufacturers. The telescopic boom will elevate microwave scatterometer system and antenna to a maximum height of 27 m, and can also be rotated through 360$^{\circ}$. A mechanism is incorporated in the system to enable tracking or monitoring angular movement of the hydraulic telescopic boom when positioned towards required target.

• Journal of the Korean Ceramic Society
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• v.27 no.1
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• pp.79-85
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• 1990

A study on the oxidation of SiO2 sensing layer was done at 950, 1000, 105$0^{\circ}C$ under dry O2 atmosphere. The rate determining step around the oxide layer thickness, 1000$\AA$ was different with the oxidation temperature, as follows ; ⅰ) linear growth at 95$0^{\circ}C$ and ⅱ) parabolic growth at 100$0^{\circ}C$ and 105$0^{\circ}C$. The flatness of SiO2 film was observed within $\pm$1% and surface state charge density was reduced by annealing in N2 atmosphere. Finally, pH sensitivity of SiO2 film, in the range of pH 3-9, was 20mV/pH.