• Applied Biological Chemistry
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• v.39 no.3
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• pp.245-248
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• 1996

Thermal stability of the major color component, cyanidin 3-glucoside, isolated from Korean pigmented rice (Oryza sativa var. Suwon 415) were investigated to explore possible application of value-added natural colors as food additives. The anthocyanin showed red and blue color with maximum absorption peaks at 511 nm and 572 nm in acidic (pH 2.0) and alkaline (pH 9.0) buffer solutions, respectively, and the thermal degradation reactions were carried out with different temperature ranges at $50{\sim}95^{\circ}C$. Degree of degradation was determined with UV/Vis spectra which indicate characteristic absorption patterns with sharp isosbestic points at 350 nm (pH 2.0), and 275, 310, and 405 nm (pH 9.0). Thus the reaction follows simple first-order kinetics. The anthocyanin was very stable against heat at acidic pH and relatively stable at alkaline pH with half-life values of 50.3 hr and 0.6 hr at $70^{\circ}C$, respectively. The activation energies and Arrhenius frequency factors of the pigment were 26.9 kcal $mol^{-1}\;and\;6.0{\times}10^{11}\;s^{-1}$, at pH 2.0, and 15.2 kcal $mol^{-1}\;and\;1.4{\times}10^{6}\;s^{-1}$, pH 9.0, and respectively.

• Applied Biological Chemistry
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• v.25 no.4
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• pp.206-210
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• 1982

Effects of pH, organic acides and UV irradiation on the browning of ginseng were investigated. Browning of ginseng tended to increase slightly in the alkaline pH range, whereas no discernible pH effect was observed in the pH ranges of 3.0-5.0 and 10.0-12.5. The treatment of fresh ginseng with organic acid such as citric acid, succinic acid, and tartaric acid greatly enhanced the browning reaction of ginseng but all treatments of mixture of glucose, glutamic acid, and glucose+glutamic acid with fumaric acid decreased the browning reaction except the treatment of fumaric acid only. The addition of ascorbic acid in concentration of greater than 0.1 M markedly accelerated the browning reaction and concurrently increased the red color, a characteristic color of quality red ginseng. Irradiation of ultraviolet light while drying red ginseng increased the brown color intensity in the first 5 days and thereafter decreased the brown color intensity.

• Applied Biological Chemistry
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• v.35 no.2
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• pp.69-75
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• 1992

Viscosity changes of acidified milk at the various pH $ranges(5.2{\sim}4.2)$was measured as a function of temperature. The rate and extent of acid-induced coagulation of milk protein were monitored by turbidity changes as a function of temperature, preheating and salt. Relative viscosities of acidified milk were also measured. The coagulation of casein occurred in a specific pH range and was accompanied by a sharp increase in viscosity at pH of $5.0{\sim}5.2$, depending on the heating temperatures. Onset pH of coagulation and maximum coagulation rate were enhanced by increasing temperatures and preheating process and reduced by addition of salt. Relative viscosity of acidified milk was reversed at the same conditions, reflecting the size of casein coagulum formed was related to the coagulation rate.

• Microbiology and Biotechnology Letters
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• v.25 no.3
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• pp.258-265
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• 1997

The effects of medium pH and culture temperature on the expression and secretion of inulinase and invertase were investigated with recombinant Saccharomyces cerevisiae cells. These cells were obtained by transformation of 2$\mu$-based plasmids pYI10 and pYS10 which contain Kluyveromyces marxianus inulinase gene (INU1A) and S. cerevisiae invertase gene (SUC2), respectively, in the downstream of GAL1 promoter. The expression level and localization of inulinase and invertase were not affected significantly by the initial medium pH: secretion efficiencies of inulinase and invertase into the medium were about 90% and 60%, respectively, in the pH ranges of 4.0 to 6.5. However, the expression and secretion of both enzymes were strongly dependent on the culture temperature. The highest expression (7.7 units/mL) and secretion (6.7 units/mL) of inulinase were observed at 28$\circ$C and 30$\circ$C. As a consequence of decreased localization of inulinase in the periplasmic space, the secretion efficiency increased from 68% at 20$\circ$C, to 95% at 35$\circ$C,. The total expression level and secretion efficiency of invertase increased from 19 units/mL and 55% at 20$\circ$C to 25 units/mL and 68% at 35$\circ$C, respectively. Irrespective of the culture temperature, the invertase activity in the cellular fraction (periplasmic space and cytoplasmic fractions) was kept constant at around 33-45%.

• Applied Biological Chemistry
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• v.30 no.1
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• pp.1-8
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• 1987

The distribution of the surface charges in two kinds of peats and their physico-chemical properties have a been studied by means of the potentiometric titration and by ion adsorption in different strength of electrolytes. The organic matter contents were 43.3% and 53.7% in Yeong Yang peat and Peong Tack peat, respectively. Their X-ray diffractograms showed that quartz was dominant in both peats, and illite, kaolinite, hydrated-halloysite and feldspars were contained in a trace. Total acidic group contents was 1.257me/g in Poeng Tack peat that was a little more than of Peong Yang heat were pKa values of Yeong Yang and Poeng Tack peat were 13.1 and 12.65, respectively. The titration curves at different ionic strength of electrolytes crossed at pH 3.9 and 4.4 in Yeong Yang peat, and pH 3.8 and 4.0 in Peong Tack peat. The pH ranges of suspensions when the net surfacecharge of the peats varied from positive to negative value were $pH\;4.55{\sim}5.20\;(NaCl)$ and $pH\;3.95{\sim}5.70\;(CaCl_2)$ in Yeong Fang peat, and $pH\;4.15{\sim}5.40\;(NaCl)$ and $pH\;3.80{\sim}4.15\;(CaCl_2)$ in Peong Tack peat. Therefore it is apparent that the zero point of charge of these peats was about pH 4.0.

• Korean journal of food and cookery science
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• v.27 no.6
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• pp.661-669
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• 2011

This study investigated the quality characteristics of saccharified minor cereal gruel prepared with different grain kojis (rice, buckwheat, sorghum, adlay, and Italian millet koji). The moisture, crude protein, crude lipid, and crude ash contents of raw materials showed ranges of 11.12 - 12.85; 5.81-16.24; 0.56 - 4.36, and 0.28 - 1.93%, respectively. The crude protein, crude lipid, and crude ash contents of the samples showed ranges of 1.64 to 2.44; 0.08 to 0.28, and 0.09 to 0.18%, respectively. The pH, L, a, and b values ranged from 6.11- 6.43; 58.72 - 65.96; 2.92 - 5.76, and 7.81- 9.42, respectively. The viscosities of saccharified minor cereal gruel prepared with different grain kojis were significantly (p<0.05) lower than the unsaccharified minor cereal gruel. After the saccharification, the soluble solids, glucose, and maltose content were significantly (p<0.05) increased, with ranges of $9.58-10.61^{\circ}Brix$; 0.64 - 0.90%, and 0.32 - 0.50%, respectively. A sensory evaluation indicated that minor cereal gruels saccharified using sorghum koji and adlay koji were more acceptable than unsaccharified minor cereal gruel. In conclusion, the cereal kojis could be used as a gruel processing method that would increase the sensory properties and nutritional values of gruels.

• Journal of Korean Society of Forest Science
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• v.87 no.2
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• pp.145-152
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• 1998

We conducted this study as a fundamental study on the response of various tree species against acid rain. The tree species used for this study were Zelkova serrata, Robinia pseudoacacia, Quercus acutissima, Prunus serrulata, Ginkgo biloba, Pinus koraiensis and Pinus densiflora. The leaves were examined for the pH changes by treatment time and the chlorophyll content into various pH solution in vitro. The results obtained were as follows ; 1. When the leaves were immersed in the solution of various pH(pH 3.0-pH 6.0) levels, the pH were changed to species specific pH ranges as pH 5.0~pH 5.5 of Z. serrata, pH 5.5~pH 6.0 of R. pseudoacacia, pH 4.5~pH 5.0 of Q. acutissima, pH 5.5~pH 6.0 of P. serrulata, pH 3.5~pH 4.5 of G. biloba, pH 3.5~pH 4.5 of P. koraiensis until 48 hours. However, in case of P. densiflora, it was difficult to find specific pH range of the species. Z. serrata, R. pseudoacacia and P. serrulata showed a little pH increasing by pH 2.0 solution treatment, while other species showed no change by the solution. 2. The amount of chlorophyll contents in Z. serrata, R. pseudoacacia and P. serrulata were decreased after immersing in the pH 2.0 solution. Chlorophyll content was almost constant in other pH levels. Other species showed almost constant chlorophyll contents in various pH levels and treatment time.

• Journal of Microbiology and Biotechnology
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• v.30 no.10
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• pp.1552-1558
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• 2020

With an increase in the consumption of non-heated fresh food, foodborne shiga toxin-producing Escherichia coli (STEC) has emerged as one of the most problematic pathogens worldwide. Endolysin, a bacteriophage-derived lysis protein, is able to lyse the target bacteria without any special resistance, and thus has been garnering interest as a powerful antimicrobial agent. In this study, rV5-like phage endolysin targeting E. coli O157:H7, named as LysECP26, was identified and purified. This endolysin had a lysozyme-like catalytic domain, but differed markedly from the sequence of lambda phage endolysin. LysECP26 exhibited strong activity with a broad lytic spectrum against various gram-negative strains (29/29) and was relatively stable at a broad temperature range (4℃-55℃). The optimum temperature and pH ranges of LysECP26 were identified at 37℃-42℃ and pH 7-8, respectively. NaCl supplementation did not affect the lytic activity. Although LysECP26 was limited in that it could not pass the outer membrane, E. coli O157: H7 could be effectively controlled by adding ethylenediaminetetraacetic acid (EDTA) and citric acid (1.44 and 1.14 log CFU/ml) within 30 min. Therefore, LysECP26 may serve as an effective biocontrol agent for gram-negative pathogens, including E. coli O157:H7.

• Journal of Environmental Health Sciences
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• v.23 no.3
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• pp.109-120
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• 1997

The purpose of this study is to evaluate the effectiveness of wastewater treatment containing surfactant. For that, comparative analysis of effectiveness of Featon Oxidation, Aluminum Sulfate, PAC (Poly Aluminum Chloride) on the treatment of the synthetic wastewater containing LAS (Linear Alkyl Sulfate), a main component of the commercial detergent was carried. Then, the optimum pH, the dosage of reagents, and the concentration of the LAS in each treatment were determined. The results of the study were summarized as following. 1. In Fenton Oxidation, optimal pH was 3 and 97.92% removal of LAS was achieved. However, the increase of the pH reduced the efficiency of LAS removal. The proper chemical dosages of FeSO$_4$ and $H_2O_2$ were 300 mg/l and the increase of dosages didn't affected the removal efficiency. Therefore, it was concluded that the economic chemical dosage was 300 mg/l of FeSO$_4$ and $H_2O_2$. 2. In case of Alum treatment, optimal pH was 11 with 61.13% removal efficiency. At other pH range, the removal efficiency was very low indicating that removal efficiency is greatly influenced by pH. The proper chemical dosage was 200 mg/l with the removal efficiency of 77.65%. The increase of chemical dosage, however, reduced the removal efficiency. 3. In case of using PAC, optimal pH was 6 with 97.99% removal efficiency. The result showed that wastewaters containing surfactant were almost completely removed at pH 6 by PAC. Removal efficiency was decreased by increasing PAC dosage higher than 400 mg/l and dosage over 700 mg/l of PAC abolished the treatment. 4. The comparative analysis of three methods revealed that the effective pH ranges were at pH 2-5 with Fenton oxidation, at pH 6-11 with PAC, and pH 11 with Alum. The removal efficiencies at these pH were 83.95-97.92%, 75.98-97.99% and 61.13%, respectively. 5. Increase in LAS concentration reduced the removal efficiencies of all three methods. In the case of PAC or Alum treatment, treatment abolished at LAS concentration higher than 700 mg/l.

• Food Science and Preservation
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• v.4 no.3
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• pp.343-349
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• 1997

The rheological Voperties of biopolymers produced by Bacilli sp. K-1 and its mutant strains(KM-21, KM-83) were studied at the temperature ranges with 20∼80$^{\circ}C$, at the concenration of 2∼6%, at the pH ranges from 3.0 to 9.0 and at the shear rate of 9.3-930sec-1 The apparent viscosity of biopolymers was decreased with increasing shear rate, and thereby biopolymers showed pseudoplastic characteristics. It was found that the apparent viscosity models respected 19 temperature, concentration and both temperature and concentration were expressed by Arrhenius Model, Exponential Model and combined of the above two Models. Therefore, the apparent viscosity could be predictable by Arrhenius and Exponential Models with high R2.