• Title/Summary/Keyword: pH effects

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Effects of pH Change by CO2 Induction and Salinity on the Hatching Rate of Artemia franciscana

  • Salma, Umme;Uddowla, Md. Hasan;Lee, Gi-Hun;Yeo, Young-Min;Kim, Hyun-Woo
    • Fisheries and Aquatic Sciences
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    • v.15 no.2
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    • pp.177-181
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    • 2012
  • To understand the effects of lower pH levels due to elevated $CO_2$ and salinity, we designed and constructed a pH-control system that included automatic $CO_2$ infusion and measured the hatching rate of a crustacean model species, Artemia franciscana. The pH-control system was cost-effective and capable of performing animal tests in which pH fluctuated around $8.0{\pm}0.1$, with the temperature around $27{\pm}0.5^{\circ}C$. Hatching rate was observed under four different pH levels (7.0, 7.3, 7.6, and untreated control) combined with three salinity ranges (15, 25, and 35 ppt). The results demonstrated that lower pH levels led to decreased hatching rates regardless of salinity, and the minimum hatching rate was detected at pH 7.0 compared to the control (pH $8.0{\pm}0.1$), supporting the idea that OA has adverse effects on hatching rates and increases the risk of juveniles being introduced in the ecosystem. In contrast, salinity changes exhibited no synergistic effects with pH and had independent effects.

The Effects of pH on Early Embryo Development of Sea Urchins (수소이온농도(pH)가 성게의 초기 배 발생에 미치는 영양)

  • 유춘만
    • Journal of Environmental Health Sciences
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    • v.30 no.2
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    • pp.104-107
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    • 2004
  • This study was conducted to evaluate the effects of pH, using gametes, embryos and early development system of three sea urchins species, Hemicentrotus pulcherrimus, Anthocidaris crassispina and Scaphechinus brevis. As the result of performing effects of pH on early embryo development, the conditions of appropriate pH on formation of normal pluteus were pH 7.0-8.0 for H. pulcherrimus, A. crassispina and S. brevis. Otherwise, the conditions of pH 5.0, pH 6.0, pH 9.0 and pH 10.0 damaged the development of early embryos of each testing animal moderately or strongly.

An Experimental Study on Some Effects of SOYANGIN-HYEONGBANGPAEDOK-SAN (소양인(少陽人) 형방패독산(刑防敗毒散)의 효능(效能)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Kim, Dae-Seong;Hong, Soon-Yong
    • Journal of Sasang Constitutional Medicine
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    • v.1 no.1
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    • pp.171-181
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    • 1989
  • We have studied some effects of the SOYANGIN-HYEONGBANGPAEDOK-SAN (S.H.P.). Several empirical remarks, depending on the different treatments, are investigated through this study as follow; 1. The Analgesic effects of S.H.P. are remarked by Acetic acid method in mice, 100 and 300mg/kg (Sample II, III), 2. The Antipyretic effect of S.H.P. are remarked by yeast method in rats, 100 and 300mg/kg (Sample II, III). 3. The inhibitory effects of S.H.P. on strychnine induced convulsion in mice is remarked. However the inhibitory effects of S.H.P. on picrotoxin induced convulsion in mice is not observed. 4. The effects of S.H.P. on muscle relaxation in mice, using the Rota-Rod test, is remarked on 1,2,3 hours by 300mg/kg (Sample III).

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Combination Effects of Benzoate, Sorbate and pH for Control of Escherichia coli O157:H7 (Escherichia coli O157:H7의 제어를 위한 Benzoate, Sorbate 및 pH의 병용처리 효과)

  • 권오진;김덕진;변명우
    • Journal of Food Hygiene and Safety
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    • v.12 no.3
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    • pp.200-204
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    • 1997
  • Effects of benzoate (0~0.6 g/$\ell$ ) and sorbate (0~2.0 g/$\ell$) on the growth of Escherichia coli O157:H7 in tryptic soy broth at various pH levels (4~8) and temperatures (4$^{\circ}C$, 37$^{\circ}C$) were investigated. Benzoate and sorbate were inhibited the growth of E. coli O157:H7 up to 12 hours cultivation at 4$^{\circ}C$, and 2.0 g/$\ell$ sorbate was only inhibited during 48 hours cultivation at 37$^{\circ}C$. Among the pH levels tested, pH 4 showed significant inhibitory effect against the E. coli O157:H7 on 4$^{\circ}C$ and at 37$^{\circ}C$, respectively. When used in combination 0.2 g/$\ell$ benzoate and sorbate were completely inhibited the growth of E. coli O157:H7 on pH 4 and at 37$^{\circ}C$. While on pH 5 at 4$^{\circ}C$, all of the concentration tested did not exert any inhibitory effect. The combined effects were retarded more than single treatment of E. coli O157:H7.

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Modulation of Cytotoxic Effects of Resveratrol by Its Anti- or Pro-oxidant Properties (Resveratrol의 항산화 및 산화촉진 활성이 세포독성에 미치는 영향)

  • Kim, Da-Ram;Hong, Jung-Il
    • Korean Journal of Food Science and Technology
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    • v.43 no.1
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    • pp.114-118
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    • 2011
  • Resveratrol is a polyphenolic compound frequently found in the diet, and its physiological actions have been extensively investigated. In the present study, modulation of the antioxidant and cytotoxic properties of resveratrol at different pHs by various antioxidants were investigated. To measure its antioxidant effects, resveratrol was incubated at different pHs, including 6.5, 7.4, and 8.0. Resveratrol incubated at pH 6.5 showed significantly higher DPPH radical scavenging activity, whereas resveratrol incubated at pH 8.0 did not show antioxidant effects. Resveratrol produced much higher amounts of $H_2O_2$ at pH 8.0 than 7.4. The cytotoxic effects of resveratrol on HeLa cells were significantly enhanced by several antioxidants, including superoxide dismutase, N-acetyl cysteine, glutathione, and ascorbic acid. The present results suggest that resveratrol shows anti- or pro-oxidant effects in different cellular organelles according to the pH conditions, and blocking of reactive oxygen species from resveratrol enhances its cytotoxic effects.

pH Effects at Doped Si Semiconductor Interfaces (Doping된 Si 반도체 세계에서 pH 효과)

  • 천장호;라극환
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.27 no.12
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    • pp.1859-1864
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    • 1990
  • The effect of H+ and OH- ion concentrations at doped Si semiconductor/pH buffer solution interfaces were investigated in terms of cyclic current-voltage characteristics. The effects of space charge on oppositely doped Si semiconductors, i.e., p-and n-Si semiconductors, can be effectively applied to study the pH effects and the slow surface states at the interfaces. The adsorptions of H+ and OH- inons on the doped Si semiconductor surfaces are physical adsorption rather than chemical adsorption. Adsorptive processes and charging effects of the slow surface states can be explained as the potential barrier variations and the related current-voltage characteristics at the interfaces. Under forward bias, the charged slow surface states on the p-and n-si semiconductor surface are donor and acceptor slow surface states, respectively. The effects of minority carriers on the slow surface states can be neglected at the doped Si semiconductor interfaces.

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The Effect of Salt and pH on the Phase Transition Behaviors of pH and Temperature-Responsive Poly(N,N-diethylacrylamide-co-methylacrylic acid)

  • Liu, Tonghuan;Fang, Jian;Zhang, Yaping;Zeng, Zhengzhi
    • Macromolecular Research
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    • v.16 no.8
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    • pp.670-675
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    • 2008
  • A series of pH and temperature-responsive (N,N-diethylacrylamide-co-methylacrylic acid) copolymers were synthesized by radical copolymerization and characterized by elemental analysis, Fourier-transform infrared (FT-IR), nuclear magnetic resonance (NMR) $^1H$, $^{13}C$ and LLS. The effects of salt and pH on the phase transition behaviors of the copolymers were investigated by uv. With increasing NaCl concentration, significant salt effects on their phase transition behaviors were observed. UV spectroscopic studies showed that the phase transition became faster with increasing NaCl concentration. In addition, the phase transition behaviors of copolymers were sensitive to pH. The pH and temperature sensitivity of these copolymers would make an interesting drug delivery system.

Effects of Different Extraction Extraction Media and Reaction Mixtures on Photosystem II Activity of Spinach Chloroplasts (시금치 엽록체의 광계의 활성에 미치는 추출용매와 반응용액의 영향)

  • 권병규
    • Journal of Plant Biology
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    • v.19 no.4
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    • pp.95-99
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    • 1976
  • This work deals with different extraction media and reaction mixtures on photosystem II activity of Spinach chloroplasts. The photoreduction rate of ferricyanide and DPIP by intact chloroplasts which extracted with four kinds of extraction media; S-Tris-N pH 7.2, 8.0, S-Tricine-N pH 7.2, 8.0, was measured in five kinds of reaction mixtures; S-Tris-N pH 7.2, 8.0, S-Tricine-N pH 7.2, 8.0, 0.05 M-Tris pH 7.2. The extraction medium which shows the highest photoreduction rate was S-Tris-N at pH 7.2 and S-Tricine-N at pH 8.0. As to the reaciton mixture, S-Tricine-N pH 8.0 showed the highest rate. On the complex effects of extraction media and reaction mixtures, the highest photordeuction rate of Hill oxidant by intact chloroplasts was obtained by S-Tris-N pH 7.2 extraction medium and S-Tricine-N pH 8.0 reaction mixture. The second highest activity was obtained by S-Tricine-N pH 8.0 extraction medium and reaction mixture.

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The Study on Emulsifying and Foaming Properties of Buckwheat Protein Isolate (분리 메밀 단백질의 유화 및 기포특성에 관한 연구)

  • 손경희;최희선
    • Korean journal of food and cookery science
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    • v.9 no.1
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    • pp.43-51
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    • 1993
  • Buckwheat protein isolate was tested for the effects of pH, addition of sodium chloride and heat treatment on solubility, emulsion capacities, emulsion stability, surface hydrophobicity, foam capacities and foam stability. The solubility of buckwheat protein isolate was affected by pH and showed the lowest value at pH 4.5, the isoelectric point of buckwheat protein isolate. The solubility significantly as the pH value reached closer to either ends of the pH, i.e., pH 1.0 and 11.0. The effects of NaCl concentration on solubility were as follows; at pH 2.0, the solubility significantly decreased when NaCl was added; at pH 4.5, it increased above 0.6 M; at pH 7.0 it increased; and at pH 9.0 it decreased. The solubility increased above $80^{\circ}C$, at all pH ranges. The emulsion capacity was the lowest at pH 4.5. It significantly increased as the pH approached higher acidic or alkalic regions. At pH 2.0, when NaCl was added, the emulsion capacity decreased, but it increased at pH 4.5 and showed the maximum value at pH 7.0 and 9.0 with 0.6 M and 0.8 M NaCl concentrations. Upon heating, the emulsion capacity decreased at acidic pH's but was maximised at pH 7.0 and 9.0 on $60^{\circ}C$ heat treatment. The emulsion stability was the lowest at pH 4.5 but increased with heat treatment. At acidic pH, the emulsion stability increased with the increase in NaCl concentration but decreased at pH 7.0 and 9.0. Generally, at other pH ranges, the emulsion stability was decreased with increased heating temperature. The surface hydrophobicity showed the highest value at pH 2.0 and the lowest value at pH 11.0. As NaCl concentrationed, the surface hydrophobicity decreased at acidic pH. The NaCl concentration had no significant effects on surface hydrophobicity at pH 7.0, 9.0 except for the highest value observed at 0.8 M and 0.4 M. At all pH ranges, the surface hydrophobicity was increased, when the temperature increased. The foam capacity decreased, with increased in pH value. At acidic pH, the foam capacity was decreased with the increased in NaCl concentration. The highest value was observed upon adding 0.2 M or 0.4 M NaCl at pH 7.0 and 9.0. Heat treatments of $60^{\circ}C$ and $40^{\circ}C$ showed the highest foam capacity values at pH 2.0 and 4.5, respectively. At pH 7.0 and 9.0, the foam capacity decreased with the increased in temperature. The foam stability was not significantly related to different pH values. The addition of 0.4 M NaCl at pH 2.0, 7.0 and 9.0 showed the highest stability and the addition of 1.0 M at pH 4.5 showed the lowest. The higher the heating temperature, the lower the foam stability at pH 2.0 and 9.0. However, the foam stability increased at pH 4.5 and 7.0 before reaching $80^{\circ}C$.

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Effects of Simulated Acid Rain on Histology, Water Status and Growth of Pinus densiflora (인공산성빗물이 소나무의 조직, 수분수지 및 생장에 미치는 영향)

  • 이창석;길지현;유영한
    • The Korean Journal of Ecology
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    • v.21 no.2
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    • pp.117-124
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    • 1998
  • To clarify the effects of acid precipitates on histological damage, water status, and growth of Pinus densiflora green house experiment applyin simulated acid rain was carried out. Contact angle of water droplet on needles of P. densiflora seedlings treated with simulated acid rain of different pHs simulated acid rain was, the more rapid transpiration was. Leaf water potential after water withdrawal was also reduced rapidly in proportion to acidity of simulated acid rain. Height growth of P. densiflora seedlings treated with simulated acid rain of pH 2 decreased, while growth of seedlings treated with that of pH 3 and 4 increased comparing with that treated with normal rain of pH 5.6. pH of cultivated soil in pH 2 plot was acidified with the amount of simulated acid rain applied but that in pH 3 and 4 plots did not show any directional change. From those results, it could be interpreted that decrease of height growth in pH 2 plot was originated from multiple effects of water deficit from rapid transpiration and soil acidification. On the other hand, increased of height growth in pH 3 and 4 plots would be originated from the supply of N and S included in simulated acid rain.

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