Browse > Article
http://dx.doi.org/10.9721/KJFST.2011.43.1.114

Modulation of Cytotoxic Effects of Resveratrol by Its Anti- or Pro-oxidant Properties  

Kim, Da-Ram (Department of Food Science and Technology, College of Natural Science, Seoul Women's University)
Hong, Jung-Il (Department of Food Science and Technology, College of Natural Science, Seoul Women's University)
Publication Information
Korean Journal of Food Science and Technology / v.43, no.1, 2011 , pp. 114-118 More about this Journal
Abstract
Resveratrol is a polyphenolic compound frequently found in the diet, and its physiological actions have been extensively investigated. In the present study, modulation of the antioxidant and cytotoxic properties of resveratrol at different pHs by various antioxidants were investigated. To measure its antioxidant effects, resveratrol was incubated at different pHs, including 6.5, 7.4, and 8.0. Resveratrol incubated at pH 6.5 showed significantly higher DPPH radical scavenging activity, whereas resveratrol incubated at pH 8.0 did not show antioxidant effects. Resveratrol produced much higher amounts of $H_2O_2$ at pH 8.0 than 7.4. The cytotoxic effects of resveratrol on HeLa cells were significantly enhanced by several antioxidants, including superoxide dismutase, N-acetyl cysteine, glutathione, and ascorbic acid. The present results suggest that resveratrol shows anti- or pro-oxidant effects in different cellular organelles according to the pH conditions, and blocking of reactive oxygen species from resveratrol enhances its cytotoxic effects.
Keywords
resveratrol; antioxidant; prooxidant; cytotoxicity; reactive oxygen species;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Frankel E, Parks E, Xu R, Schneeman B, Davis P, German J. Effect of n-3 fatty acid-rich fish oil supplementation on the oxidation of low density lipoproteins. Lipids 29: 233-236 (1994)   DOI   ScienceOn
2 Bertelli AA, Giovannini L, Giannessi D, Migliori M, Bernini W, Fregoni M, Bertelli A. Antiplatelet activity of synthetic and natural resveratrol in red wine. Int. J. Tissue React. 17: 1-3 (1995)
3 Stojanovi S, Sprinz H, Brede O. Efficiency and mechanism of the antioxidant action of trans-resveratrol and its analogues in the radical liposome oxidation. Arch. Biochem. Biophys. 391: 79-89 (2001)   DOI   ScienceOn
4 Murcia MA, Martinez-Tome M. Antioxidant activity of resveratrol compared with common food additives. J. Food Protect. 64: 379-384 (2001)
5 Gehm BD, McAndrews JM, Chien PY, Jameson JL. Resveratrol, a polyphenolic compound found in grapes and wine, is an agonist for the estrogen receptor. Proc. Natl. Acad. Sci. USA 94: 14138-14143 (1997)   DOI   ScienceOn
6 Bhat KPL, Pezzuto JM. Resveratrol exhibits cytostatic and antiestrogenic properties with human endometrial adenocarcinoma (Ishikawa) cells. Cancer Res. 61: 6137-6144 (2001)
7 Holmes-McNary M, Baldwin AS. Chemopreventive properties of trans-resveratrol are associated with inhibition of activation of the $I{\kappa}B$ kinase. Cancer Res. 60: 3477-3483 (2000)
8 Lobo RA. Benefits and risks of estrogen replacement therapy 1. Obstet. Gynecol. 173: 982-989 (1995)   DOI   ScienceOn
9 Kopp P. Resveratrol, a phytoestrogen found in red wine. A possible explanation for the conundrum of the 'French paradox'. Eur. J. Endocrinol. 138: 619-620 (1998)   DOI   ScienceOn
10 Soleas GJ, Diamandis EP, Goldberg DM. Resveratrol: A molecule whose time has come? and gone? Clin. Biochem. 30: 91-113 (1997)   DOI   ScienceOn
11 Alarcon de la Lastra C, Villegas I. Resveratrol as an antioxidant and pro-oxidant agent: Mechanisms and clinical implications. Biochem. Soc. T. 35: 1156-1160 (2007)   DOI   ScienceOn
12 Boonstra J, Post JA. Molecular events associated with reactive oxygen species and cell cycle progression in mammalian cells. Gene 337: 1-13 (2004)   DOI
13 Hou Z, Sang S, You H, Lee MJ, Hong J, Chin KV, Yang CS. Mechanism of action of (-)-epigallocatechin-3-gallate: Auto-oxidation- dependent inactivation of epidermal growth factor receptor and direct effects on growth inhibition in human esophageal cancer KYSE 150 cells. Cancer Res. 65: 8049-8056 (2005)
14 Leonard SS, Xia C, Jiang BH, Stinefelt B, Klandorf H, Harris GK, Shi X. Resveratrol scavenges reactive oxygen species and effects radical-induced cellular responses. Biochem. Bioph. Res. Co. 309: 1017-1026 (2003)   DOI   ScienceOn
15 Losa G. Resveratrol modulates apoptosis and oxidation in human blood mononuclear cells. Eur. J. Clin. Invest. 33: 818-823 (2003)   DOI   ScienceOn
16 Juan ME, Wenzel U, Daniel H, Planas JM. Resveratrol induces apoptosis through ROS-dependent mitochondria pathway in HT- 29 human colorectal carcinoma cells. J. Agr. Food Chem. 56: 4813-4818 (2008)   DOI   ScienceOn
17 Ozgova S, Hermanek J, Gut I. Different antioxidant effects of polyphenols on lipid peroxidation and hydroxyl radicals in the NADPH-, Fe-ascorbate-and Fe-microsomal systems. Biochem. Pharmacol. 66: 1127-1137 (2003)   DOI   ScienceOn
18 Demaurex N. pH homeostasis of cellular organelles. News Physiol. Sci. 17: 1-5 (2002)
19 Waslidge NB, Hayes DJ. A colorimetric method for the determination of lipoxygenase activity suitable for use in a high throughput assay format. Anal. Biochem. 231: 354-358 (1995)   DOI   ScienceOn
20 Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 26: 1699-1203 (1958)
21 Zini R, Morin C, Bertelli A, Bertelli AA, Tillement JP. Effects of resveratrol on the rat brain respiratory chain. Drug Exp. Clin. Res. 25: 87-97 (1999)
22 Ahmad KA, Clement V, Pervaiz S. Pro-oxidant activity of low doses of resveratrol inhibits hydrogen peroxide-induced apoptosis. Ann. NY Acad. Sci. 1010: 365-373 (2003)   DOI   ScienceOn
23 Mahal H, Mukherjee T. Scavenging of reactive oxygen radicals by resveratrol: Antioxidant effect. Res. Chem. Intermediat. 32:59-71 (2006)   DOI   ScienceOn
24 Martinez J, Moreno JJ. Effect of resveratrol, a natural polyphenolic compound, on reactive oxygen species and prostaglandin production. Biochem. Pharmacol. 59: 865-870 (2000)   DOI   ScienceOn
25 Gusman J, Malonne H, Atassi G. A reappraisal of the potential chemopreventive and chemotherapeutic properties of resveratrol. Carcinogenesis 22: 1111-1117 (2001)   DOI
26 Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CWW, Fong HHS, Farnsworth NR, Kinghorn AD, Mehta RG. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275: 218-220 (1997)   DOI