• Title/Summary/Keyword: pH change

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Arginase Activity in Response to pH Change in Canavalia Lineata Callus (해녀콩 캘러스에서 pH 변화에 따른 Arginase의 활성)

  • 이주용
    • Journal of Plant Biology
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    • v.36 no.4
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    • pp.407-413
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    • 1993
  • 해녀콩(Canavalia lineata L. DC) 잎에서 유도한 캘러스를 pH 8인 완충용액에 한 시간 동안 처리하면 pH 6에서 보다 arginase의 활성이 약 두 배로 증가했다. 캘러스에서 얻은 arginase의 조효소액을 Sephacryl S-200 컬럼에서 분획하면 분자량이 각각 380 kD과 179 kD으로 나타났는데 분자량이 큰 arginase의 분획은 pH 8 처리구에서 각각 상대적으로 많이 나타났다. 그리고 pH 6에 0.5 mM Mn2+를 첨가하였을 때도 380 kD arginase의 분획이 크게 나타났으며, pH 6 처리에서 얻은 179 kD arginase 분획에 pH 8 처리를 하면 380 kD 분획으로 쉽게 전이될 수 있었다. pH 6 처리 후 추출한 arginase는 Mn2+의 첨가로 활성이 크게 증가하여 pH 8 처리 후 추출한 arginase와 비슷한 활성을 보이나, pH 8 처리 후 추출한 arginase는 Mn2+의 첨가로 더 이상의 활성증가를 보이지 않았다. Mn2+이 없는 조건에서 두 arginase의 Km값과 Vmax를 조사한 결과, 380 kD arginase는 22 mM과 1.61 $\mu$mole urea.min-1.mg-1 protein, 그리고 179 kD arginase는 30 mM과 0.79 $\mu$mole urea.min-1.mg-1 protein으로 측정되었다.

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The Study on the Surface State of Mg2Ni Exposed to Air by a Chemical Treatment (화학처리방법을 이용한 공기중에 노출된 Mg2Ni의 표면상태에 관한 연구)

  • Han, Jeong-Seop
    • Transactions of the Korean hydrogen and new energy society
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    • v.6 no.2
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    • pp.91-100
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    • 1995
  • To investigate the surface state of $Mg_2Ni$ which was exposed to the air, a chemical treatment was undertaken with $H_2SO_4$ solution. During chemical treatment, the change of pH was measured continuously and the chemically treated specimen was hydrided to study the effect of chemical treatment on the hydrogenation. The pH changing behavior with the various $H_2SO_4$ concentration appeared very diffemrently. Especially in the solution including 3CC $H_2SO_4$, the behavior of pH change can be divided 3 steps. It is also shown that the $Mg_2Ni$ chemically treated with $H_2SO_4$ can be hydrided even under room temperature. By the SEM observation the was reasion that after chemical treatment the surface of a particle was covered with Ni layer.

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Study on the Reactivity of Sodium Phosphate Ammonium Chloride pH Reduction Agents (인산나트륨계 염화암모늄 pH저감제의 반응성 검토)

  • Shin, Ki-Don;Gao, San;Lee, Gun-Cheol
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2017.11a
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    • pp.105-106
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    • 2017
  • Previous studies have confirmed the performance of pH reduction agents using liquid sodium phosphate based ammonium chloride as a pH reduction agent. In this study, the pH reduction performance considering economical and applicability as a practical stage and the property change analysis for the identification of the reaction mechanism of the pH reduction agent were carried out. As a result, the pH reduction performance at a low rate of the pH reducing agent was confirmed. The specific gravity of CaO decreased significantly after XRF analysis. It is also believed that this reduces the amount of Ca(OH)2 produced and contributes to pH reduction.

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pH equation model of RFID-pH sensor using fermented foods (RFID-pH 센서를 이용한 발효식품의 pH 모델식)

  • Lee, Chang Won;Kim, Joo Woong;Son, Dong Sul;Eom, Ki-Hwan
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2013.05a
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    • pp.849-852
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    • 2013
  • These fermented food has been increased interest to people's health in recent years. Factors which have taste, storability and trophism of fermented food is affected food of material, temperature, humidity and pH. But fermented food has yet not been established of model equation for the change in pH. If they that seller and consumer can know the status and quality, customer could increase credibility and consumption-oriented about fermented foods. In this paper, we obtain model equation through measured PH of fermented foods. So, model equation is offered platform about maturity of fermented foods. In order to confirm the usefulness of the proposed model equation, we tested the change in PH about a kimchi and a rice wine which is fermented food of Korea representative.

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A Study of Sex Difference by Chemical Determination of Dentin in Human Teeth (치아경조직의 화학적 판정에 의한 성차 연구)

  • 장세활;김종열
    • Journal of Oral Medicine and Pain
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    • v.8 no.1
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    • pp.127-142
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    • 1983
  • This thesis was to investigate sex defferencd by employing the methodes of Bernadskij's and it's modification by means of the evaluation of pH of dentin in 162 extracted teeth. The obtained were as follows: 1. In 72% of all experinments, pH of human dentin substance was higher in female than in male. Mean pH was 13.175±0.031 in male and 13.245±0.030 in female. 2. After comparative studies on each pH in anatomical region of tooth arrangement, the sex difference of pH was most obviously showed in molars. 3. In pH change according to ages, the 30's was the highest in male and the 20's in female, also pH showed decresing tendency by the increasing of age in both male and female. 4. There was no pH difference in anatomical regions of tooth arrangment. 5. Mean pH was 13.282±0.013 in under 50 years of age and 13.282±0.022 over 50. It proved that pH was higher in under 50 years of age than in over 50.

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Molasses clarifying method used by lime-phosphate for yeast culture (효모배지용 당밀의 청징법에 관하여)

  • 임억규
    • Korean Journal of Microbiology
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    • v.19 no.3
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    • pp.121-127
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    • 1981
  • The constituents of molasses and effect of pH precipitate formation in molasses solution, vary according to its producing districts. The formation of precipitation is not so changeable in the range of buffering zone of molasses solution(pH4.3-6.3) in philippine molasses according to the change of pH value. On lower or higher than the range of buffering zone, the precipitation is increased from pH 4.3 to 2.8 and from 6.3 to 8.1, it is decreased when pH value is lower or higher than the pH value range. For molasses clarifying, it had better adjust the pH of molasses solution to neutral or weak alkali range out of the alkai side of the buffering zone, with lime solution. And then, add the calcium super phosphate solution to pH value of alkali side in buffering zone, as much as the pH of clarified molasses solution can reach to middle value in buffering zone. For the equilibrium of pH value on clarifying molasses, it takes plenty of time more than 6 hours.

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Micro pH Sensor Using Patterned Hydrogel with pH Indicators

  • Jang, Ji-Sung;Kwon, Sung-Hoon
    • Journal of Sensor Science and Technology
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    • v.20 no.4
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    • pp.234-237
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    • 2011
  • This paper presents a study into pH Indicator-Embedded hydrogel micro-particles which are encoded various shapes according to the captured indicator. We incorporate various pH indicators into a photo-curable hydrogel, PEG-DA(Poly(ethylene glycol) diacrylate). Using the latest fluidic lithography techniques, we can easily fabricate variously patterned hydrogel particles based on in-situ photopolymerization of the PEG-DA in a micro-fluidic channel. The shape of the particle is related to the pH indicator inside the particle. We demonstrate that the micro pH sensors change their colors according to the pH levels. The micro pH sensors have various characteristics that are related to the curing time, particle size, etc. By changing these conditions, we can adjust the long term stability and reaction time of the hybrid micro pH sensors.

The Effects of Light and $CO_2$ on the Changes of Electrical Potential Difference in Isolated Epidermis and Intact Leaves of Commeina communis L

  • Lee Joon-Sang
    • Korean Journal of Environmental Biology
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    • v.23 no.3 s.59
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    • pp.221-227
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    • 2005
  • The effects of light and $CO_2$ on the electrophysiological characteristics of guard cells in the intact leaf and isolated epidermis have been investigated. Fast hyperpolarization of guard cell apoplastic PD in the intact leaf was recorded reaching up to around 7 mV and 20 mV in response to light and $CO_2$. Whenever the experiments were attempted with isolated epidermis, there was no response to light and $CO_2$. In order to determine the influence of the mesophyll cells, the apoplastic PD of guard cells in isolated epidermis was measured in the presence of the mesophyll supernatant or the control medium. The apoplastic PD in isolated epidermis was hyperpolarized to -7mV, changing from -22mV to -29mV at 40 min. But, when isolated epidermis was incubated with the supernatant from mesophyll cells incubated in the light, the apoplastic PD in isolated epidermis was hyperpolarized to -19 mV, changing from -22 mV to -40.5 mV. $CO_2$ also caused a change of 0.1 to 0.3 pH unit in the intact leaf. However, this change was absent in isolated epidermis. A vibrating probe was used to detect the change in electrical currents at the surface of excised intact leaves and isolated epidermis. The reading of excised intact leaves in the dark was $0.5\muA\;cm^{-2},$ remaining steady until illuminated. Light increased the current on the surface of excised leaves to about $0.8\muA\;cm^{-2},$. However, light had no effect in the current on the surface of isolated epidermis. Apoplastic pH changes across the stomatal complex in response to light and dark were measured both in the intact leaves and isolated epidermis over the same time period using pH micro-electrodes. The guard cell wall of intact leaf was acidified to 2.5 pH unit, falling from pH 7.5 to pH 5.0 in the first 10 min. in the light. At the same time the guard cell wall pH of isolated epidermis fell from pH 7.5 to pH 7.0 at 10 min. The guard cell wall pH of isolated epidermis incubated in the mesophyll supernatant fell from pH 7.6 to pH 6.7 at 10 min. Likewise, It could be imagined that an electrical signal, chemicals and hormones propagated from the mesophyll in response to light and $CO_2$ could control a fast stomatal response.

ALKALINITY CHANGE IN ROOT DENTIN FOLLOWING CALCIUM HYDROXIDE DRESSING (수종 수산화칼슘 제재를 이용한 근관충전 후 치근상아질에서의 염기도 변화)

  • Lee, Kyung-Ha;Ahn, Yun-Sil;Lee, Kwang-Won;Lee, Myung-Jong
    • Restorative Dentistry and Endodontics
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    • v.22 no.2
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    • pp.670-679
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    • 1997
  • The pH changes in 4 small cavities prepared at the facial inner dentin and lingual outer dentin of the cervical and apical portion of root filled with calcium hydroxide pastes were investigated. Forty extracted permanent teeth with single canal were instrumented with step-back method, and then 4 small cavities were prepared. Two inner dentin cavities were cut a distance of about 1.0mm from the canal wall and two outer dentin cavities were cut to a depth of about 0.5mm from the root surface. Root canals and prepared cavities were flushed with 17% EDTA, and then irrigated with 5% NaOCl to remove smear layer. Teeth were randomly divided into four groups. Control group was not filled and the remaining other groups were filled with mixture of calcium hydroxide and distilled water, Vitapex$^{(R)}$ paste and Pulpdent$^{(R)}$ paste respectively. The pH change of the dentin in each cavity was measured at 0, 1, 3, 7, 14, 21, 28, 60, 90 days with pH microelectrode(WPI Co., USA). The results were as follows : 1. The groups obturated with Pulpdent$^{(R)}$ paste and Aqueous calcium hydroxide produced the increased pH level at 1 day and maintained plateau over next 3weeks and decreased after 3weeks. 2. The group obturated with Vitapex$^{(R)}$ paste observed no significant pH change until 2weeks and slight increased pH at 3weeks and sequential increasing after 3weeks. But, the pH in the group obturated with Vitapex$^{(R)}$ paste remained significantly below the pH measured in the other two experimental groups(P<0.05). 3. All experimental groups showed pH level similar to control group after 28 days. 4. The pH of outer dentin is slightly higher than that of inner dentin. There is no significant difference in pH level between apical and cervical dentin throughout the duration of the experiment, though apical dentin showed slightly higher pH than cervical dentin at 1 day(P<0.05).

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A study on the relationship between concentration of phosphorus, turbidity, and pH in water and soil (물과 토양에서 인의 농도, 탁도 그리고 pH와의 관계에 관한 연구)

  • Min, Young-Hong;Hyun, Dae-Yoeung;Eum, Chul-Hun;Lee, Seung-Ho
    • Analytical Science and Technology
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    • v.24 no.4
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    • pp.304-309
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    • 2011
  • In this research, behaviour of turbidity and phosphorus in water and soil dependent upon pH and a change of water was studied. Phosphorus dissolve rate from turbidity was increased for water if potential of hydrogen was less than pH 4 or more than pH 7. Turbidity release rate from soil was increased with pH. Turbidity release rate from soil was drastically increased for water if potential of hydrogen was more than pH 4. turbidity release rate from soil was stabilized more than pH 6. Dissolved phosphorus was increased from 2 hours to 24 hours and stabilized in 24 hours. Turbidity was reached the peak of 24 hours and decreased from 24 hours to 96 hours. Turbidity and dissolved phosphorus was decreased for water if these samples were changed a overlying water. Behaviour of turbidity was analogous to dissolved phosphorus when potential of hydrogen was increased from pH 6 to pH 10 and a change of overlying water was increased from 1 time to 4 times. These results suggest that phosphorus dissolve rate and turbidity were directiy correlated with pH. These results are of great importance in lakes because most lakes have a pH in the range of pH 7-10.