• 한국식품영양학회지
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• 제14권5호
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• pp.418-422
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• 2001

시판되는 4개 사 고춧가루를 구입하여 고춧가루의 품질에 관한 이화학적 특성을 비교 분석하였다. 고춧가루 선호도를 결정하는 것은 색상인데 C사 제품의 L값이 46.667로 가장 높았으며 a, b값도 22.738과 19.941로 제일 높게 나왔다. 고추의 적색소인 capsanthin은 D사가 80.0mg/100g으로 가장 많았으며 A, C, B사가 각각 75.0, 58.9, 51.0mg/100g 순이었고 ASTA color 도 같은 경 향을 보였다. Capsanthin 양과는 달리 L, a, b값 모두 C사가 제일 높게 나왔는데 이는 고춧가루의 입도에 의한 영향 때문으로 여겨진다 각 고춧가루의 pH 차이는 크지 않았으며 유기산은 A, B, D사 제품에서 quinic acid가 가장 많이 검출되었으며 C사 제품은citric acid가 가장 높게 검출되었다. 신미성분 가운데 capsaicin은 D사가 59.93mg/100g으로 가장 많았으며 B사가 25.87mg/100g으로 제일 적었으며 capsaicinoids는 각각 62.93, 28.20mg/100g로 나타나 2배 이상의 차이를 보였다. 미생물의 발생 가능성을 추정할 수 있는 수분활성 도는 A, B, C, D사가 각각 0.584, 0.676, 0.526 및 0.568 로 나타나 A, C, D사 제품은 문제가 없을 것으로 예측되나 B사 제품의 수분활성도가 0.676으로 곰팡이 발생 우려가 있을 것으로 예측되었다.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1073-1081
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• 2024

Obesity is spawned by an inequality between the portion of energy consumed and the quantity of energy expended. Disease entities such as cardiovascular disease, arteriosclerosis, hypertension, and cancer, which are correlated with obesity, influence society and the economy. Suppression of adipogenesis, the process of white adipocyte generation, remains a promising approach for treating obesity. Oil Red O staining was used to differentiate 3T3-L1 cells for screening 20 distinct Lactobacillus species. Among these, Lactobacillus acidophilus DS0079, referred to as YBS1, was selected for further study. YBS1 therapy decreased 3T3-L1 cell development. Triglyceride accumulation and mRNA expression of the primary adipogenic marker, peroxisome proliferator-activated receptor gamma (PPARγ), including its downstream target genes, adipocyte fatty acid binding protein 4 and adiponectin, were almost eliminated. YBS1 inhibited adipocyte differentiation at the early stage (days 0-2), but no significant difference was noted between the mid-stage (days 2-4) and late-stage (days 4-6) development. YBS1 stimulated the activation of p38 mitogen-activated protein kinase (p38 MAPK) during the early stages of adipogenesis; however, this effect was eliminated by the SB203580 inhibitor. The data showed that YBS1 administration inhibited the initial development of adipocytes via stimulation of the p38 MAPK signaling pathway, which in turn controlled PPARγ expression. In summary, YBS1 has potential efficacy as an anti-obesity supplement and requires further exploration.

• 한국토양비료학회지
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• 제29권4호
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• pp.396-402
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• 1996

식물생육촉진 유용미생물을 지리산 일대의 침엽수와 활엽수 밑의 부엽토(腐葉土)에서 선발하고, 고추에 접종하였을때 고추 묘소질과 생육 및 수량에 미치는 영향에 대한 결과를 요약하면 다음과 같다. 1. 혼합배양결과 Pseudomonas P배지는 $25^{\circ}C$에 Micrococcus sp., Enterobacter agglomerans, Pseudomonas putida, Pseudomonas fluorescens등 4균주, Sabouraud dextrose배지에서는 $35^{\circ}C$에서 Bacillus subtilis, Cellulomonas sp., Bacillus megaterium, Staphylococcus xylosus등 4균주의 생육이 양호하였다. 2. 혼합균주 처리구는 무처리구에 비해 초장, 분지수등은 증가하였고, EC 수준이 높아질수록 초장은 작아진 반면, 분지수는 혼합균주 처리 58일(日) 이후 EC 2.9ds/m에 비해 EC 8.6 dS/m에서 많아지는 경향이었다. 수량은 혼합균주 처리구가 무처리구에 비해 13% 증수되었고, EC 수준이 높아질수록 감소하는 경향이었다. 3. 시험후 토양의 EC, $P_2O_5$, K, Ca, Mg, $NO_3-N$은 EC 수준이 높아질수록 현저하게 증가하는 경향이었으며, 혼합균주 처리구는 무처리구에 비해 OM 및 $NH_4{^+}-N$은 낮은 반면, EC, $P_2O_5$, K, Ca, Mg, $NO_3-N$은 높은 경향이었다. 4. 시험후 혼합균주 처리구는 무처리구에 비해 수분율이 높은 경향이었으며, EC 수준이 높아 질수록 가밀도 수분율, 공극율 등은 증가하는 경향이었다. 5. 혼합균주 처리구는 무처리구에 비해 과실과 엽에서 T-N, $P_2O_5$, $K_2O$, CaO, MgO함량이 높았으며, EC 수준이 높아질수록 $P_2O_5$, $K_2O$, MgO 함량은 증가한 반면, CaO 함량은 낮아지는 경향이었다.

• Archives of Pharmacal Research
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• 제27권9호
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• pp.906-911
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• 2004

In order to evaluate estrogenic compounds in natural products, an in vitro detection system was established. For this system, the human breast cancer cell line MCF7 was stably trans-fected using an estrogen responsive chloramphenicol acetyltransferase (CAT) reporter plas-mid yielding MCF7/pDsCAT-ERE119-Ad2MLP cells. To test the estrogenic responsiveness of this in vitro assay system, MCF7/pDsCAT-ERE119-Ad2MLP cells were treated with various concentrations of 17f3-estradiol. Treatments of 10$^{-8}$ to 10$^{-12}$ M 17$\beta$-estradiol revealed significant concentration dependent estrogenic activities compared with ethanol. We used in vitro assay system to detect estrogenic effects in Puerariae radix and Ginseng radix Rubra extracts. Treat-ment of 500 and 50 $\mu\textrm{g}$/ml of Puerariae radix extracts increased the transcriptional activity approximately 4- and 1.5-fold, respectively, compared with the ethanol treatment. Treatment of 500, 50, and 5 $\mu\textrm{g}$/ml of Ginseng radix Rubra extracts increased the transcriptional activity approximately 3.2-,2.7, and 1.4-fold, respectively, compared with the ethanol treatment. These observations suggest that Puerariae radix and Ginseng radix Rubra extracts have effective estrogenic actions and that they could be developed as estrogenic supplements.

• 성인간호학회지
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• 제23권2호
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• pp.111-122
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• 2011

Purpose: The aim on this study was to establish the validity, reliability and efficiency of a Pain Self-Report Scale for elderly with dementia and compare these results with an observational pain rating scale. Methods: Study subjects were 136 elderly with dementia who were residents in a nursing home, geriatric hospital, or day care center. The subject's pain was measured by five self-report scales and observational scale. DS-DAT (discomfort scale-dementia of the Alzheimer's type) was used for pain behavior observational measure. Cognitive state was assessed using the MMSE (Mini-Mental State Examination). Results: Observational rating correlated moderately with self-report (r=.225~.585, p<.05) and tended to underestimate pain intensity. Test-retest reliability was high for all five self-report scales, and the correlation between these scales was very strong (r=.735~.856, p<.05). Comprehension rate of VDS (verbal descriptor scale) was 88.3%, and NRS (numeric rating scale) 69.9%, FPS (face pain scale) 66.9%, HVAS (horizontal visual analog scale) and VVAS (vertical visual analog scale) 65.4%. Conclusion: Nurses should not apply observational scales routinely in demented patients as many of these are capable of reporting their own pain. Self-report, the highest standard of pain measurement can be reliably performed in a large proportion of demented elderly.

• Journal of Microbiology and Biotechnology
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• 제25권4호
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• pp.503-510
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• 2015

The iLOV protein belongs to a family of blue-light photoreceptor proteins containing a light-oxygen-voltage sensing domain with a noncovalently bound flavin mononucleotide (FMN) as its chromophore. Owing to advantages such as its small size, oxygen-independent nature, and pH stability, iLOV is an ideal candidate over other reporter fluorescent proteins such as GFP and DsRed. Here, for the first time, we describe the feasibility of applying LOV domain-based fluorescent iLOV as a metal sensor by measuring the fluorescence quenching of a protein with respect to the concentration of metal ions. In the present study, we demonstrated the inherent copper sensing property of the iLOV protein and identified the possible amino acids responsible for metal binding. The fluorescence quenching upon exposure to Cu²⁺ was highly sensitive and exhibited reversibility upon the addition of the metal chelator EDTA. The copper binding constant was found to be 4.72 ± 0.84 µM. In addition, Cu²⁺-bound iLOV showed high fluorescence quenching at near physiological pH. Further computational analysis yielded a better insight into understanding the possible amino acids responsible for Cu²⁺ binding with the iLOV protein.

• 한국균학회지
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• 제26권3호통권86호
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• pp.387-395
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• 1998

여러가지 기주에서 유쾌한 Calonectria ilicicola 균주의 병원성에 대한 형태적, 유전적 표지를 검출하기 위하여 균사생장, 균핵, 자낭각 그리고 dsRNA를 조사하였다. 시험된 모든 균주에서 병원성, 균핵수, 자낭각수 와 균사생장 등의 다양한 변이가 관찰되었으나 dsRNA는 검출되지 않았다. 균의 병원성은 균핵과 자낭각 생산과 각각 정의 상관관계가 있었으나, 균사 생장은 자낭각의 생산과 부의 상관관계를 보여 주었다. 땅콩에서 유래한 균주보다 콩에서 유래한 균주가 기주인 콩에 더 강한 병원성을 보였으며 균핵과 자낭각의 생산도 더 많았다. 이와 같은 결과로 볼 때 C. ilicicla의 균핵과 자낭각 생산력은 inoculum potential의 구성요소로써 작용하며, 이중 균의 자낭각 생산력은 병원성과 기주 분화를 나타내는데 유용한 표지로써 이용될 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제27권6호
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• pp.1098-1105
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• 2017

Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones ($Mut^s$) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.

• Asian-Australasian Journal of Animal Sciences
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• 제22권4호
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• pp.492-499
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• 2009

A Silkie Bantam embryo fibroblast line (named SBF59 line) was successfully established by using direct explant culture and cryopreservation techniques. Cell morphology, viability, dynamic growth and contamination were tested and the karyotype and levels of isoenzymes of lactic dehydrogenase and malic dehydrogenase were analyzed. Four kinds of fluorescent protein extrogenes, including $pEGFP-N_3$, $pECFP-N_1$, $pEYFP-N_1$ and $pDsRed1-N_1$ were transfected into the cells. The results showed that the cells were healthy and possessed a fibrous structure without a change in morphology. The average viability of the cells was 96% before freezing and 90.5% after thawing. The growth curve appeared as typical "S" shape and the cell growth passed through a detention phase, a logarithmic phase and a platform phase; the estimated population doubling time (PDT) was 38.5 h; assays for the presence of bacteria, fungi, viruses and mycoplasmas were negative; the cell line showed no cross contamination when assessed by isoenzyme analysis; the chromosome number was 2n = 78 on more than 88% of occasions; four kinds of fluorescent protein extro-genes appeared to be expressed effectively with a high transfection efficiency between 18.3% and 42.3%. The cell line met the required quality control standard. It not only preserves the genetic resources of the important Silkie Bantam at the cellular level but also provides valuable materials for genomic, post-genomic, somatic cell cloning research and other applications.

• The Korean Journal of Ecology
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• 제19권5호
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• pp.417-430
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• 1996

This study was conducted to investigate the developmental process of plant community during the secondary succession and the dynamics of soil properties in the burned and unburned areas of Mt. Ch’oly-san. Owing to the forest fire occurred on April, 1989, the red pine(Pinus densiflora) forest and its floor vegetation were burned down. The floristic composition of burned and unburned areas were composed of 53 and 49 species of vascular plants, respectively. The dominant species based on SDR4 of the burned sites were lespedeza cyrtobotrya (89.62), Miscanthus sinensis var. purpurascens (62.50), and Carex humilis (58.73), Quercus serrata (43.33). In contrast, Pinus densiflora (83.56), Lespedeza cyrtobotrya (55.57), Miscanthus sinensis var. purpurascens (51.88) and Carex humilis (50.41) were dominant in the unburned area. The biological spectra showed the $H-D_1-R_5-e$ type in both the burned and unburned areas. The indices of similarity ($CC_S$) between the two areas were 0.74. Degree of succession (DS) was 604 in the burned area and 802 in the unburned area. From these facts, it is assumed that the succession is rapidly progressing because of the recovery of vegetation. The species diversity ($\={H}$) and evenness index(C) in the burned and unburned areas were 0.15 and 0.18, respectively. Red pine tree did not resprout after scorch by the forest fire, but Lespedeza, Quercus, Rhododendron, Albizzia, and Zanthoxylum resprouted from the roots and trunks after the forest fire. It seems that these species are the fire-resistant species. Soil properties such as soil pH, content of organic matter, available phosphous, total nitrogen, tatal carbon, exchangeable potssium, sodium, calcium, and magnesium increased due to forest fire. These results suggest the intensity of forest fire in the study area was relatively weak. Monthly changes of soil properties were of little significance except for some cases.