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Establishment of In Vitro Test System for the Evaluation of the Estrogenic Activities of Natural Products  

Kim, Ok-Soo (Department of Bioscience and Biotechnology, Silla University)
Choi, Jung-Hye (Division of Civil and Environmental Engineering, Korean Maritime University)
Soung, Young-Hwa (Department of Pathology, College of Medicine, The Catholic University of Korea)
Lee, Seon-Hee (Department of Bioscience and Biotechnology, Silla University)
Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Silla University)
Ha, Jong-Myung (Department of Bioscience and Biotechnology, Silla University)
Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University)
Heo, Moon-Soo (Faculty of Applied Marine Science, Cheju National University)
Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University)
Publication Information
Archives of Pharmacal Research / v.27, no.9, 2004 , pp. 906-911 More about this Journal
Abstract
In order to evaluate estrogenic compounds in natural products, an in vitro detection system was established. For this system, the human breast cancer cell line MCF7 was stably trans-fected using an estrogen responsive chloramphenicol acetyltransferase (CAT) reporter plas-mid yielding MCF7/pDsCAT-ERE119-Ad2MLP cells. To test the estrogenic responsiveness of this in vitro assay system, MCF7/pDsCAT-ERE119-Ad2MLP cells were treated with various concentrations of 17f3-estradiol. Treatments of 10$^{-8}$ to 10$^{-12}$ M 17$\beta$-estradiol revealed significant concentration dependent estrogenic activities compared with ethanol. We used in vitro assay system to detect estrogenic effects in Puerariae radix and Ginseng radix Rubra extracts. Treat-ment of 500 and 50 $\mu\textrm{g}$/ml of Puerariae radix extracts increased the transcriptional activity approximately 4- and 1.5-fold, respectively, compared with the ethanol treatment. Treatment of 500, 50, and 5 $\mu\textrm{g}$/ml of Ginseng radix Rubra extracts increased the transcriptional activity approximately 3.2-,2.7, and 1.4-fold, respectively, compared with the ethanol treatment. These observations suggest that Puerariae radix and Ginseng radix Rubra extracts have effective estrogenic actions and that they could be developed as estrogenic supplements.
Keywords
Chloramphenicol acetyltransferase (CAT）; Estrogen; Isoflavone; Ginseng radix Rubra; Korea red ginseng; MCF7 cells; Phytoestrogen; Puerariae radix;
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