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Establishment of In Vitro Test System for the Evaluation of the Estrogenic Activities of Natural Products  

Kim, Ok-Soo (Department of Bioscience and Biotechnology, Silla University)
Choi, Jung-Hye (Division of Civil and Environmental Engineering, Korean Maritime University)
Soung, Young-Hwa (Department of Pathology, College of Medicine, The Catholic University of Korea)
Lee, Seon-Hee (Department of Bioscience and Biotechnology, Silla University)
Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Silla University)
Ha, Jong-Myung (Department of Bioscience and Biotechnology, Silla University)
Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University)
Heo, Moon-Soo (Faculty of Applied Marine Science, Cheju National University)
Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University)
Publication Information
Archives of Pharmacal Research / v.27, no.9, 2004 , pp. 906-911 More about this Journal
Abstract
In order to evaluate estrogenic compounds in natural products, an in vitro detection system was established. For this system, the human breast cancer cell line MCF7 was stably trans-fected using an estrogen responsive chloramphenicol acetyltransferase (CAT) reporter plas-mid yielding MCF7/pDsCAT-ERE119-Ad2MLP cells. To test the estrogenic responsiveness of this in vitro assay system, MCF7/pDsCAT-ERE119-Ad2MLP cells were treated with various concentrations of 17f3-estradiol. Treatments of 10$^{-8}$ to 10$^{-12}$ M 17$\beta$-estradiol revealed significant concentration dependent estrogenic activities compared with ethanol. We used in vitro assay system to detect estrogenic effects in Puerariae radix and Ginseng radix Rubra extracts. Treat-ment of 500 and 50 $\mu\textrm{g}$/ml of Puerariae radix extracts increased the transcriptional activity approximately 4- and 1.5-fold, respectively, compared with the ethanol treatment. Treatment of 500, 50, and 5 $\mu\textrm{g}$/ml of Ginseng radix Rubra extracts increased the transcriptional activity approximately 3.2-,2.7, and 1.4-fold, respectively, compared with the ethanol treatment. These observations suggest that Puerariae radix and Ginseng radix Rubra extracts have effective estrogenic actions and that they could be developed as estrogenic supplements.
Keywords
Chloramphenicol acetyltransferase (CAT）; Estrogen; Isoflavone; Ginseng radix Rubra; Korea red ginseng; MCF7 cells; Phytoestrogen; Puerariae radix;
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1 Arao, T., Udayama, M., Kinjo, J., and Nohara, T., Preventive effects of saponins from the Pueraria lobata root on in vitro immunological liver injury of rat primary hepatocyte cultures. Planta Med., 64, 413-416 (1998)   DOI   ScienceOn
2 Diel, P., Smolnikar, K., and Michna, H., In vitro test systems for the evaluation of the estrogenic activity of natural products. Planta Med., 65, 197-203 (1999)   DOI   ScienceOn
3 Farnsworth, N. R., Bingel, A. S., Cordell, G. A., Crane, F. A., and Fong, H. H. S., Potential value of plants as sources of new antifertility agents II. J. Pahrm. Sci., 64, 717-754 (1975)   DOI   PUBMED
4 Hirakura, K., Morita, M., Nakajima, K., Sugama, K., Takagi, K., Niitsu, K., Ikeya, Y., Maruno, M., and Okada, M., Phenolic glucosides from the root of Pueraria lobata. Phytochemistry, 46, 921-928 (1997)   DOI   ScienceOn
5 Howes, M. J., Perry, N. S., and Houghton, P. J., Plants with traditional uses and activities, relevant to the management of Alzheimer's disease and other cognitive disorders. Phytother. Res., 17, 1-18 (2003)   DOI   ScienceOn
6 Knight, D. C. and Eden, J. A., A review of the clinical effects of phytoestrogens. Obstet. Gynecol., 87, 897-904 (1996)
7 Lephart, E. D., West, T. W., Weber, K. S., Rhees, R. W., Setchell, K. D., Adlercreutz, H., and Lund, T. D., Neurobehavioral effects of dietary soy phytoestrogens. Neurotoxicol. Teratol., 24, 5-16 (2002)   DOI   ScienceOn
8 Miyamoto, N. G., Moncollin, V., Egly, J. M., and Chambon, P., Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter. EMBO J., 4, 3565-3570 (1985)
9 Price, K. R. and Fenwich, G. R., Naturally occurring oestrogens in foods-a review. Food Add. Contam., 2, 73-106 (1985)   DOI   PUBMED   ScienceOn
10 Xuan, B., Zhou, Y. H., Yang, R. L., Li, N., Min, Z. D., and Chiou, G. C., Improvement of ocular blood flow and retinal functions with puerarin analogs. J. Ocul. Pharmacol. Ther., 15, 207-216 (1999)   DOI   ScienceOn
11 Zhao, L., Chen, Q., and Diaz Brinton, R., Neuroprotective and neurotrophic efficacy of phytoestrogens in cultured hippocampal neurons. Exp. Biol. Med.(Maywood), 227, 509-519 (2002)   DOI
12 Amato, P., Christophe, S., and Mellon, P. L., Estrogenic activity of herbs commonly used as remedies for menopausal symptoms. Menopause, 9, 145-150 (2002)   DOI   PUBMED   ScienceOn
13 Nah, S. Y., Park, H. J., and McCleskey, E. W., A trace component of ginseng that inhibits Ca$Ca^{2+}$ channels through a pertussis toxin-sensitive G protein. Proc. Natl. Acad. Sci. U.S.A., 92, 8739-8743 (1995)   DOI   ScienceOn
14 Barnes, S., Effect of genistein on in vitro and in vivo models of cancer. J. Nutr., 125, 777S-783S (1995)
15 Keung, W. M. and Vallee, B. L., Daidzin and its antidipsotropic analogs inhibit serotonin and dopamine metabolism in isolated mitochondria. Proc. Natl. Acad. Sci. U.S.A., 95, 2198-2203 (1998a)   DOI   ScienceOn
16 Liu, W. K., Xu, S. X., and Che, C. T., Anti-proliferative effect of ginseng saponins on human prostate cancer cell line. Life Sci., 67, 1297-1306 (2000)   DOI   ScienceOn
17 Lin, R. C., Guthrie, S., Xie, C. Y, Mai, K., Lee, D. Y., Lumeng, L., and Li, T. K., Isoflavonoid compounds extracted from Pueraria lobata suppress alcohol preference in a pharmacogenetic rat model of alcoholism. Alcohol Clin. Exp. Res., 20, 659-663 (1996)   DOI   ScienceOn
18 Cao, X., Tian, Y., Zhang, T., Li, X., and Ito, Y., Separation and purification of isoflavones from Pueraria lobata by high-speed counter-current chromatography. J. Chromatogr. A., 855, 709-713 (1999)   DOI   PUBMED   ScienceOn
19 Auborn, K. J., Fan, S., Rosen, E. M., Goodwin, L., Chandraskaren, A., Williams, D. E., Chen, D., and Carter, T. H., Indole-3-carbinol is a negative regulator of estrogen. J. Nutr., 133, 2470S-2475S (2003)   DOI
20 Lee, H. P., Gourley, L., Duffy, S. W., Esteve, J., Lee, J., and Day, N. E., Dietary effects on breast-cancer risk in Singapore. Lancet, 337, 1197-1200 (1991)   DOI   ScienceOn
21 Adlercreutz, H., Does fiber-rich food containing animal lignan precursors protect against both colon and breast cancer? An extension of the 'fiber hypothesis.' Gastroenterology, 86, 761-764 (1984)   PUBMED
22 Whitten, P. L. and Patisaul, H. B., Cross-species and interassay com-parisons of phytoestrogen action. Environ. Health Perspect, 109 Suppl 1, 5-20 (2001)   DOI   ScienceOn
23 Whitten, P. L. and Naftolin, F., Dietary estrogens-a biologically active background for estrogen action. In New Biology of Steroid Hormones(Hochberg RB, Naftolin F, eds), 155-167, Ravel Press, New York, NY (1991)
24 Ponglikitmongkol, M., White, J. H., and Chambon, P., Synergistic activation of transcription by the human estrogen receptor bound to tandem responsive elements. EMBO J., 9, 2221-2231 (1990)   PUBMED
25 Adlercreutz, H., Fotsis, T., Heikkinen, R., Dwyer, J. T., Woods, M., Goldin, B. R., and Gorbach, S. L., Excretion of the lignans enterolactone and enterodiol and of equol in omnivorous and vegetarian women and in women with breast cancer. Lancet, 2, 1295-1299(1982)   PUBMED
26 Anderson, J. J. and Garner, S. C., Phytoestrogens and bone. Baillieres. Clin. Endocrinol. Metab., 12, 543-557 (1998)   DOI   ScienceOn
27 Lin, R. C. and Li, T. K., Effects of isoflavones on alcohol pharmacokinetics and alcohol-drinking behaviour in rats. Am. J. Clin. Nutr., 68, 1512S-1515S (1998)   DOI
28 Melis, G. B., Paoletti, A. M., Bartolini, R., Tosti Balducci, M., Massi, G. B., Bruni, V., Becorpi, A., Ottanelli, S., Floretti, P., and Gambacciani, M., Isoflavone and low doses of estrogens in the prevention of bone mineral loss in climacterium. Bone Miner., 19, Suppl 1:S49-S56 (1992)   DOI   ScienceOn
29 Anderson, J. J., Ambrose, W. W., and Garner, S. C., Biphasic effects of genistein on bone tissue in the ovariectomized, lactating rat model. Proc. Soc. Exp. Biol. Med., 217, 345-350 (1998)   DOI
30 Setchell, K. D. R., Lawson, A. M., Borriello, S. P., Harkness, R., Gordon, H., Morgan, D. M. L. and Kirk, D. N., Adlercreutz, H., Anderson, L. C., Axelson, M., Lignan formation in manmicrobial involvement and possible roles in relation to cancer. Lancet, 2, 4-7 (1981)   PUBMED
31 Bronstein, I., Fortin, J., Stanley, P. E., Stewart, G. S., and Kricka, L. J., Chemiluminescent and bioluminescent reporter gene assays. Anal. Biochem., 219, 169-181 (1994)   DOI   ScienceOn
32 Jung, N. P. and Jin, S. H., Studies on the physiological and biochemical effects of Korean ginseng. Kor. J. Ginseng Sci., 20, 431-471 (2000)
33 Keung, W. M. and Vallee, B. L., Kudzu root: an ancient Chinese source of modern antidipsotropic agents. Phytochemistry, 47, 499-506 (1998b)   DOI   ScienceOn
34 Guerra, M. C., Speroni, E., Broccoli, M., Cangini, M., Pasini, P., Minghett, A., Crespi-Perellino, N., Mirasoli, M., Cantelli-Forti, G., and Paolini, M., Comparison between Chinese medical herb Pueraria lobata crude extract and its main isoflavone puerarin antioxidant properties and effects on rat liver CYP-catalysed drug metabolism. Life Sci., 67, 2997-3006 (2000)   DOI   ScienceOn