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The Clinical Usefulness of Cystatin C in Evaluating Renal Function in Children with Various Renal Diseases (다양한 신질환을 가진 소아에서 Cystatin C 검사의 임상적 유용성)

  • Kim, Khi-Joo;Kim, Joung-A;Shin, Jae-Il;Hwang, You-Sik;Cheung, Il-Chun;Lim, Jong-Baeck;Lee, Jae-Seung
    • Childhood Kidney Diseases
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    • v.11 no.2
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    • pp.161-167
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    • 2007
  • Purpose : GFR(glomerular filtration rate) is a fundamental parameter in detecting renal impairment and predicts the progression of renal disease. Because serum creatinine has several disadvantages, serum cystatin C has been recently proposed as a new endogenous marker for GFR. We compared serum cystatin C with creatinine and creatinine clearance to investigate the clinical usefulness of cystatin C. Methods : We retrospectively analyzed 46 patients(60 case numbers) who had various renal diseases and classified them into 3 groups according to creatinine clearance(Group 1 : CrCl <40 mL/min/$1.73m^2$, Group 2 : CrCl 40-60 mL/min/$1.73m^2$, Group 3 CrCl >60 mL/min/$1.73m^2$). We measured serum creatinine, cystatin C and creatinine clearance and also analyzed the correlations among them. Results : Serum cystatin C and creatinine showed a similar correlation to creatinine clearance (r=0.685, r=0.640, respectively) and showed similar diagnostic accuracy in detecting decreased GFR(AUC, cystatin C 0.829 vs. creatinine 0.826, P=0.848). Serum cystatin C showed a greater sensitivity for detecting a decreased GFR than creatinine in Group 2 and 3(Group 1 : 100% vs. 100%, Group 2 : 70% vs. 35%, Group 3 : 46% vs. 15%). Conclusions : Serum cystatin C could be a useful endogenous marker for GFR and would be superior to serum creatinine in early detection of renal impairment in pediatric patients with renal diseases.

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Influences of Extraction pH on the Functionality of Soybean Protein Isolate (추출 pH가 분리대두단백질의 기능성에 미치는 영향)

  • Bae, Song-Hwan;Rhee, Chul
    • Korean Journal of Food Science and Technology
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    • v.30 no.3
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    • pp.557-561
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    • 1998
  • This study was conducted to investigate the functionality of soybean protein isolates extracted in acidic range (pH 2.0 and 3.0), neutral range (pH 7.0) and alkaline range (pH 10.0 and 12.0). The protein content of soybean protein isolates extracted at pH 3.0 was maximum (93.31%), but that of pH 7.0 was minimum (73.93%). The extraction yield of soybean protein isolates extracted at pH 3.0 was minimum (0.36%), but that of pH 12.0 was maximum (47.54%). The functionality (solubility, water absorption, oil absorption, foam capacity, foam stability, emulsion capacity and gelation) of soybean protein isolates was significantly influenced by pH of extraction medium. The soybean protein isolates extracted at pH 2.0 and 3.0 were more soluble at acidic ranges and those of pH 3.0 and 7.0 were more soluble at neutral ranges, but those of pH 2.0, 3.0, 7.0, 10.0 and 12.0 were more soluble at alkaline ranges than other ranges. The soybean protein isolates extracted at pH 2.0 and pH 12.0 gave greater water absorption, oil absorption and foam capacity than those extracted at pH 3.0, pH 7.0 and pH 10.0. And the emulsion capacity of soybean protein isolates was increased by the increase of extraction pH.

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Controlled Release of Oxyfluorfen from the Variously Complexed Formulations - II. Selection of Promising Formulations (수종(數種) 결합제형(結合劑型)으로부터 Oxyfluorfen의 방출제어(放出制御) 연구(硏究) - II. 유용(有用)한 방출제어형(放出制御型의) 선발(選拔))

  • Guh, J.O.;Im, W.H.;Lim, K.P.;Cho, C.S.;Chung, I.W.
    • Korean Journal of Weed Science
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    • v.10 no.3
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    • pp.207-213
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    • 1990
  • Thirty formulations of oxyfluorfen(2-chloro-2,2,2-trifluoro-p-toyl 3-ethoxy-4-nitrophenyl ether were manufactured by adsorption and substitation. Of them 16 formulations n-ere selected because they showed rekatuvely high activity and long durability in the bioassay with (Brassica campestris V.L.) Then, the formulations selected and 4 commercial formulations were tested using Monochoria in pots. As the result, the formulations of polyer 11, elvan 7/40, coal-slag 7/40, zeolite(A), and bentonite(A) were selected because they showed almost complete inhibition of Monochoria even up the 73 days after treatment.

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Analysis of the Relationships Between Sasang Typology, Holland's Vocational Typology, and Myers-Brigg's Types Among Undergraduate Students at the College of Oriental Medicine

  • Lee, Soo-Jin;Park, Suzanne-H;Im, Jung-Hyeok;Sin, Ye-Sul;Ha, Hyon-Il;Shin, Sang-Woo;Kwon, Young-Kyu;Chae, Han
    • The Journal of Korean Medicine
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    • v.28 no.4
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    • pp.61-68
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    • 2007
  • Objectives : The present study investigated the integrative relationships between Sasang typology, Holland's vocational typology, and Myers-Briggs type. Methods : The sample was composed of 83 sophomores at the College of Oriental Medicine, Daegu Hanny University (56 men, 27 women; ages 19 to 39, mean age ${\pm}$S. D. = 24.38 ${\pm}$ 5.28) and was carried out with the QSCC II, Holland inventory, and MBTI. SPSS 12.0 was employed for statistical analyses. Results : The Sasang types of the subjects were as followed: 21 Soyangin (10 men, 11 women) (25.3%), 20 Taeumin (18 men, 2 women) (24.1%), and 42 Soeumin (28 men, 14 women) (50.6%). There were no significant differences in the mean scores of Realistic, Investigative, Artistic, Social, Enterprising, or Conventional scale between the 3 Sasang types, but in the mean scores of Realistic, Investigative, and Artistic scale between the 4 MBTI combinations (Sensing-Thinking, Sensing-Feeling, Intuition-Thinking, and Intuition-Feeling type): F (3, 73) = 3.11, p < .05 in Realistic scale, F (2, 73) = 3.70, p < .05 in Investigative scale, and F (2, 73) = 5.60, p < .01 in Artistic scale. Conclusions : The present study discovered that the first preference for vocational aptitude of undergraduate students at the College of Oriental Medicinewas Investigative and the second preference was Artistic, which fitted Holland's vocational codes as Investigative/Artistic or Investigative/Social scale. The personality traits underlying Sasang typology play an important factor in making career decisions.

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Comparative study of off-clamp, laparoscopic partial nephrectomy (OCLPN) and conventional hilar control, laparoscopic partial nephrectomy (HCLPN) for renal tumors: One-year follow-up results of renal function change

  • Kang, Su Hwan;Rhew, Hyun Yul;Kim, Taek Sang
    • Kosin Medical Journal
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    • v.33 no.2
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    • pp.191-199
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    • 2018
  • Objectives: We designed the study to compare the oncologic and renal function outcomes of off-clamp, laparoscopic partial nephrectomy (OCLPN) and conventional laparoscopic partial nephrectomy (HCLPN) for renal tumors. Methods: Between March 2008 and July 2015, 114 patients who underwent laparoscopic partial nephrectomy (LPN) of a renal neoplasm were studied. We performed LPN without hilar clamp on 40 patients (OCLPN, Group 1), and conventional LPN with hilar control and renorrhaphy on another 40 patients (HCLPN, Group 2). We retrospectively reviewed the medical records of each patient's age, sex, R.E.N.A.L. nephrometry score (RNS), operation time, complications, hospitalization period, tumor size, positive resection margin, histologic classification of tumor, pathologic stage, Fuhrman grade, estimated blood loss (EBL), warm ischemic time (WIT), and estimated glomerular filtration rate (eGFR) before and one year after surgery. Results: There were no significant differences in age, sex, preoperative eGFR, EBL, surgical (anesthesia) time, and tumor size between the two groups. The mean eGFR was not significantly different between the OCLPN and HCLPN groups 1 month (95 and $86.2mL/min/1.73m^2$, respectively; P = 0.106), 6 months (92.9 and $83.6mL/min/1.73m^2$, respectively; P = 0.151) and 12 months (93.8 and $84.7mL/min/1.73m^2$, respectively; P = 0.077) postoperatively. The change in eGFR after one year was 3.9% in the OCLPN group and -7.9% in the HCLPN group. Conclusions: OCLPN was superior to HCLPN in preserving renal function one year after surgery, and there was no statistically significant difference in tumor treatment results.

Studies on Quality of Silage from Domestic Herbages IV. Effects of starch addition on the quality of leaf and stem silage of kudzu (야초 사일리지의 품질향상에 관한 연구 III. 칡 사일리지에 있어서 잎과 줄기의 전분첨가효과)

  • 김종쾌;이상훈;김영규;김대진
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.11 no.1
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    • pp.60-67
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    • 1991
  • This experiment was conducted to investigate the effects of starch addition on the fermentative quality and dry matter digestibility(DMD) of Kudzu(Puerari~ thzmbergii Bentham) silage. The herbages was ensiled by the conventional methods in small plastic silo of 7.5 liters with addition of starch of 0 %, 2 %, 4 74, 6 % and 8 %, respectivery. The samples of kudzu silage were separated into leaf and stem, and was determined the pH, organic acid and characteristics of fiber such as neutral detergent fiber(NDF), acid detergent liber(ADF) and acid detergent lignin(ADL). The DMD of leaf and stem silage were evaluated by pepsin-cellulase technique method. The energy values(tota1 digestible nutrients, TDN; digestible energy, DE; metaboliz;~bie energy. ME) were calculated by DMD. The results obtained were as follows: 1. The fiberous meterials(such as NDF, AIIF and ADL) of leaf and stem were decreased with increasing levels of starch(p

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Effect of the Convergent Mock Interview Program on the Program Demand, Career Attitude Maturity, Job Preparation Stress (융합적 모의면접프로그램이 프로그램 요구도, 진로태도성숙도, 취업준비스트레스에 미치는 영향)

  • Joung, Hye-Young;Sim, Sun-Sook;Lee, Sang-Bok
    • Journal of the Korea Convergence Society
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    • v.8 no.9
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    • pp.59-65
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    • 2017
  • The purpose of this study is to investigate the effects of the convergent mock interview program on college students who are preparing for employment and their effects on program demand, career attitude maturity and job preparation stress. The study subjects were 73 nursing students and pre - 4th grade college students. Data collection was done before and after the application of the program. The collected data were analyzed using the SPSS WIN 23.0 program. The results of the study showed that there was a statistically significant difference between the program demand (t = 7.92, p <.001) and career attitude maturity (t = 3.35, p = .001).but Job stress (t = .98, p = .327) was not found to have any effect Based on these results, we propose to increase the program period and to develop and apply a customized convergence program tailored to the characteristics of the students.

Overproduction of Lignin Peroxidase from Phanerochaete chrysosporium PSBL-1 (Phanerochaete chrysosporium PSBL-1의 배양조건 최적화를 통한 Lignin Peroxidase의 과량생산)

  • 정병철;한윤전;장승욱;정욱진;원유정
    • Korean Journal of Microbiology
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    • v.36 no.3
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    • pp.228-235
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    • 2000
  • Until now, it was diIliculi to overproduce lignin peroxidase(LiP) fiom Pl~anemchaete ch~ysosporium since the lack of optimized growth conditions. In this paper, we optimized the LIP production conditions and monitored LIP isozyines of fl chqsospoi.ium PSBL-1. The optimized condition includes sponge matrix support, no addition of $MnSO_4$, excess addition of niixogen source(48 inM diarmnonium), and addition of stabilizer(2 mM verakyl alcohol). Finally we obtained Lip activity of 1,800 unitsll. HI isozyne was overproduced when inyceliuin was cultivated in media containing $Mn^{2+}$ (2.73 inM) and excess nitrogen(48 11d4 diannnonium). Three azo dyes(acid yellow 9, congo ued, orange IT; each concenimtion of50 $\mu$M) we1-e rapidly decolorized within 2 inins by 0.4 un~t or Lip.

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Localization and isozyme patterns of phosphatase in Fibricola seoulensis (Fibricola seoulensis에서 phosphatase의 분포와 동위효소유형)

  • 김홍자;김창환
    • Parasites, Hosts and Diseases
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    • v.31 no.4
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    • pp.353-362
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    • 1993
  • The present study was carried out to investigate the localization and isozyme patterns of acid phosphatase and alkaline phosphatase in metacercariae and in adults of F. seoulensis by enzyme-histochemistry method and electrophoresis. Acidphosphatase showed a strong activity at pH 5 in the intestinal caecum of adults, but showed no reactions in the nonsubstrate control and in the inhibitor-treated control. Alkaline phosphatase showed a strong activity at pH 8 in the intestinal caecum and the tribocytic organ of adults, and in the intestinal caecum and in the genital anlagen of metacercariae. In non-denature PAGE, ten bands of protein fraction from the extracts of metacercariae and twenty-two bands from adults were detected. In denature PAGE, two protein bands having molecular weights of 192 kDa and 123 kDa were detected in the metacercariae, but absent from adult stage. In adults, protein fractions of 27.5 kDa, 24.5 kDa, 21.4 kDa, 18 kDa, 16 kDa and 15 kDa were detected. In non-denature PAGE, isozymes of acid phosphatase showed the most strong activity at pH 5, whereas no activity was shown at pH 2 and pH 7. One isozyme 85 kDa, 73 kDa and 62 kDa) in adults.

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Purification and Pathogenicity of the Granulosis Viruses of Common Cabbage Worm, Pieris rapae and Pieris brassicae (배추흰나비 과립병바이러스의 정제 및 병원성)

  • 류강선;김근영;강석원
    • Korean journal of applied entomology
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    • v.30 no.3
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    • pp.187-195
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    • 1991
  • This study was carried out to acquire some basic biological informations on the granulosis virus (GV) of Pieris rapae and Pieris brassicae. Purified fractions of GV capsules in an sucrose density gradient centrifugation yielded on homogenous and sharp peak without a shoulder. Electron microscopy revealed that GV capsules were mostly ovalglove in shape. P. rapae and P. rapae GV isolated from P. rapae comprised granules($396\pm38\times238\pm25nm$ for P. rapae GV. $375\pm40\times255\pm28nm$ for P. brassicae GV) which contained single virus particle. The virus particles were 250- $275{\times}63$ -73nm for P. rapae GV and 243-250 $\times$ 63-75nm for P. brassicae GV containing a nucleocapsid 225 $\times$ 31nm for P. rapae, 225 $\times$ 29nm for P. brassicae within an envelope. The virulent difference between the two viruses was very small in their virulence for P. rapae larvae showing the $LC_{50}$( -log) with 5.5673 for P. rapae GV and 5.8104 for P. brassicae GV. Also the $LT_{50}$ of the 3rd instar P. rapae larvae against $10^{-6}$ inoculum was 8.17 days for P. rapae GV and 7.16 days for P. brassicae GV.

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