• Journal of Microbiology and Biotechnology
• /
• 제22권5호
• /
• pp.592-599
• /
• 2012

Bacillus licheniformis ${\alpha}$-amylase (BLA), a thermophilic counterpart of Bacillus amyloliquefaciens ${\alpha}$-amylase (BAA), is an appropriate model for the design of stabilizing mutations in BAA. BLA has 10 more histidines than BAA. Considering this prominent difference, in the present study, three out of these positions (I34, Q67, and P407; located in the thermostability determinant 1 region and Ca-III binding site of BAA) were replaced with histidine in BAA, using the site-directed mutagenesis technique. The results showed that the thermostability of P407H and Q67H mutants had increased, but no significant changes were observed in their kinetic parameters compared to that of the wild type. I34H replacement resulted in complete loss of enzyme activity. Moreover, fluorescence and circular dichroism data indicated a more rigid structure for the P407H variant compared with that of the wild-type BAA. However, the flexibility of Q67H and I34H mutants increased in comparison with that of wild-type enzyme.

• Journal of Microbiology
• /
• 제45권4호
• /
• pp.344-349
• /
• 2007

We have isolated previously three synthetic lethal mutants in Schizosaccharomyces pombe, which genetically interact with mex67, in order to identify the genes involved in mRNA export. A novel nup97 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex3. The nup97 gene contains one intron and encodes an 851 amino-acid protein that is similar to nucleoporins, Nppl06p in S. pombe and Nic96p in Saccharomyces cerevisiae. The nup97 gene is essential for vegetative growth, and nup97 null mutant harboring pREP41X-Nup97 showed $poly(A)^+$ RNA export defect when expression of nup97 is repressed in the presence of thiamine. These results suggest that nup97 is involved in mRNA export from the nucleus to cytoplasm.

• 한국치위생학회지
• /
• 제11권2호
• /
• pp.213-222
• /
• 2011

Objectives : The purpose of this study is to arrange basic data necessary for education of AIDS and to prevent AIDS on undergraduates' knowledge or attitude toward AIDS. Methods : A self-administered questionnaire survey was used among 350 undergraduates in some of Jeonbuk and Gyeongnam area from September 9, 2009 to December 10, 2009 and then obtained the following results. Results : 1. Information route on AIDS was indicated to be higher in order of school education with 37.1% and TV & newspaper with 31.5% as for the health-related department. The health-unrelated department was indicated to be the highest in TV & newspaper with 67 people(43.8%), and had statistically significant difference(p<0.01). 2. The whole knowledge score on AIDS was indicated to be $12.72{\pm}2.67$ for the health-related department, resulting having been higher than the health-unrelated department with $11.84{\pm}3.30$. There was significant difference(p<0.01). The attitude score on AIDS was indicated to be $4.85{\pm}1.67$ for the health-related department, resulting having been higher than the health-unrelated department with $4.38{\pm}1.58$. There was significant difference(p<0.01). 3. Knowledge and attitude toward general AIDS stood at r=.112(p<0.05), thereby having had positive correlation. Knowledge and attitude toward AIDS infection stood at r=-.220(p<0.01), thereby having had negative correlation. Relationship between the whole knowledge and attitude toward AIDS stood at r=-.105(p<0.05), thereby having had negative correlation. Conclusions : AIDS is exponentially increasing in the whole world and is getting more serious. Even in our country, it is the tendency that the infected are gradually increasing. Health care of AIDS, which is impossible to be fully recovered and has no vaccine, is preferential to prevent infection. A continuous and efficient preventive education program is thought to be necessary for straightening wrong knowledge and prejudice on this by grasping right knowledge and attitude toward AIDS.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권8호
• /
• pp.3555-3559
• /
• 2014

Background: The triple-negative breast cancer (TNBC) is an aggressive cancer characterized by the absence of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2). Preoperative mammography and ultrasound features of TNBC may potentially suggest characteristics of the disease and assist in treatment decisions. Materials and Methods: The study covered 153 patients with TNBC from May 2011 to May 2012 who were confirmed by postoperative pathology results in our hospital. We compared the radiological findings among the patients and sought to determine the significant iconographic features. The biomarkers p53 and Ki-67 are regarded as significant factors in TNBC. They were therefore used to divide the TNBC into four groups for assessment of relationships with TNBC imaging features. Results: On mammography, most TNBCs exhibit obscure (44.3%) masses. On ultrasound, the majority of masses (95.4%) were predominantly indistinct (50.7%), irregular (76.0%) or featuring posterior echo enhancement/shadowing. Color Doppler flow imaging (CDFI) emphasized hypervascular (32.9%) masses. Differences in CDFI by ultrasound among the four groups were statistically significant (p=0.009). There were obvious differences in the percentages of spiculated margin (p=0.049) and intensive posterior echo (p=0.006) with spotty flow imaging by ultrasound between the Ki-67 (+) p53 (+) and other groups. Conclusions: A combination of mammography and ultrasound revealed the imaging characteristics of TNBC included an obscure mass with less attenuated posterior echoes and some vascularity. A worse prognosis was associated with spiculated margin and intensive posterior echoes with spotty flow imaging.

• Archives of Plastic Surgery
• /
• 제42권1호
• /
• pp.59-67
• /
• 2015

Background Negative-pressure wound therapy (NPWT) induces angiogenesis and collagen synthesis to promote tissue healing. Although acetic acid soaks normalize alkali wound conditions to raise tissue oxygen saturation and deconstruct the biofilms of chronic wounds, frequent dressing changes are required. Methods Combined use of NPWT and acetic acid irrigation was assessed in the treatment of chronic wounds, instilling acetic acid solution (1%) beneath polyurethane membranes twice daily for three weeks under continuous pressure (125 mm Hg). Clinical photographs, pH levels, cultures, and debrided fragments of wounds were obtained pre- and posttreatment. Tissue immunostaining (CD31, Ki-67, and CD45) and reverse transcription-polymerase chain reaction (vascular endothelial growth factor [VEGF], vascular endothelial growth factor receptor [VEGFR]; procollagen; hypoxia-inducible factor 1 alpha [HIF-1-alpha]; matrix metalloproteinase [MMP]-1,-3,-9; and tissue inhibitor of metalloproteinase [TIMP]) were also performed. Results Wound sizes tended to diminish with the combined therapy, accompanied by drops in wound pH (weakly acidic or neutral) and less evidence of infection. CD31 and Ki-67 immunostaining increased (P<0.05) post-treatment, as did the levels of VEGFR, procollagen, and MMP-1 (P<0.05), whereas the VEGF, HIF-1-alpha, and MMP-9/TIMP levels declined (P<0.05). Conclusions By combining acetic acid irrigation with negative-pressure dressings, both the pH and the size of chronic wounds can be reduced and infections be controlled. This approach may enhance angiogenesis and collagen synthesis in wounds, restoring the extracellular matrix.

• The Korean Journal of Physiology and Pharmacology
• /
• 제14권5호
• /
• pp.325-329
• /
• 2010

Vascular NADPH oxidase plays a pivotal role in producing superoxide in endothelial cells and thus acts in the initiation and development of inflammatory cardiovascular diseases such as atherosclerosis. Epigallocatechin-3-gallate (EGCG), the major catechin derived from green tea, has multiple beneficial effects for treating cardiovascular disease but the effect of EGCG on the expression of vascular NADPH oxidase remains unknown. In this study, we investigated the mechanism(s) by which EGCG might inhibit the expression of subunits of NADPH oxidase, namely $p47^{phox}$, $p67^{phox}$ and $p22^{phox}$, induced by angiotensin II (Ang II) in human umbilical vein endothelial cells. Ang II increased the expression levels of $p47^{phox}$, $p67^{phox}$, and $p22^{phox}$, but EGCG counteracted this effect on $p47^{phox}$. Moreover, EGCG did not affect the production of reactive oxygen species induced by Ang II. These data suggest a novel mechanism whereby EGCG might provide direct vascular benefits for treating inflammatory cardiovascular diseases.

• 한국세라믹학회지
• /
• 제41권1호
• /
• pp.45-50
• /
• 2004

Ca/P 비가 각각 1.62, 1.67, 1.72인 수산화아파타이트(Hydroxyapatite, HA) 세라믹스를 제조하고, HA 표면에서 수용액에 의하여 일어나는 용해현상을 고찰하였다. 수용액에서 3일간 침적시킨 경우 입계에서부터 용해가 시작되었으며, 침적시간이 10일로 증가하면서 이러한 입계용해에 의한 HA 세라믹스의 미세구조적 분해 현상은 비화학양론적 조성, 즉 Ca/P 비가 1.62 또는 1.72인 시편에서 두드러지게 나타났다. 주목할만한 것은, 용해가 진행되는 동안 세라믹스의 표면에는 골식세포의 흡수 과정에서 흔히 볼 수 있는 표면 결함(lacunae)과 매우 유사한 모양을 갖는 원형의 빈 공간(cavity)이 형성되었다.

• 한국식생활문화학회지
• /
• 제38권5호
• /
• pp.345-355
• /
• 2023

This study was conducted on four types of laver soup prepared using recipes from Gangwon-do (GW), Gyeongsangnam-do (GN), Jeollanam-do (JN), and the media (MD). Salinities, soluble solids, free amino acids, color values, turbidities, and pH values of soups were measured, and sensory evaluations were done. pH values were significantly higher for GW and GN recipes than the JN and MD recipes (p<0.05). The salinities of JN and MD were significantly higher than those of the other soups (p<0.05). Regarding soluble solids, JN had the highest at 2.64°Brix, whereas GW had the lowest at 0.86 °Brix (p<0.05). GW and JN had higher concentrations of valine, phenylalanine, and glutamic acid (p<0.05). JN had significantly higher redness (a) than the other soups but significantly lower lightness (L) and yellowness (b) (p<0.05). Regarding transmittance, GW had a significantly higher value (67.67%T) than the other soups. JN and MD had higher acceptability results than GW or GN in terms of flavor, mouthfeel, and overall acceptability. Sensory intensity evaluations revealed that JN and MD had higher "soy sauce aroma" and "saltiness" and a greater "broth turbidity" than the other soups. These results indicate that the physicochemical and sensory characteristics of regional laver soups differ.

• 한국자기학회지
• /
• 제12권1호
• /
• pp.14-19
• /
• 2002

CMR 자성물질인 LSMO 페롭스카이트 망간산화물에 $^{57}$ Fe를 미량 치환한 L $a_{0.67}$S $r_{0.33}$M $n_{0.99}$$^{57}$ F $e_{0.01}$ $O_3$물질에 대하여 그 결정학적 및 자기적 특성을 x-선 회절 실험, 진동시료 자화율 측정 실험, 자기저항 실험, Mossbauer 분광 실험을 통하여 연구하였다. 물을 용매로 한 sol-gel법을 이용하여 단일상의 polycrystalline L $a_{0.67}$S $r_{0.33}$M $n_{0.99}$$^{57}$ F $e_{0.01}$ $O_3$분말시료를 합성하였으며, 결정구조는 격자 상수 $a_{0}$ =5.480 $\AA$, $\alpha$=60.259$^{\circ}$를 갖는 rhombohedral구조로 분석되었다. 상온에서의 magnetic moment 값은 60.15 emu/g임을 알 수 있었고 큐리온도( $T_{c}$)는 375 K로 결정하였다. Mossbauer 스펙트럼은 20-400 K 영역에서 측정되었으며, 온도증가에 따라 관측되는 비대칭적 선폭증가는 이방성 초미세자기장 요동 모델로 설명할 수 있었다. 이 때 이방성 초미세 자기장이 + $H_{0}$ 및 - $H_{0}$ 로 진동하는 시간 비율은 $P_{+}$= 0.80 및 $P_{-}$= 0.20으로 분석되었다. 이방성 초미세자기장 요동 진동수로부터 온도변화에 따른 이방성 에너지를 계산하였으며, 150 K에서 124.01 erg/$cm^3$로 최대값을 갖음을 알 수 있었다.다.었다.었다.다.었다.

• 인간식물환경학회지
• /
• 제23권2호
• /
• pp.211-220
• /
• 2020

The purpose of this study was to analyze the frequency and contents of horticultural activities in 696 individual activities listed in 11 teacher's guidebooks of Nuri Curriculum for 5-year-olds, and to find out the perceptions of horticultural activities in the formal curriculum. The target horticultural activities that were selected were those using natural objects like potted plants, water, wind, soil, stones, etc. as the topic or subject of activities, and those with different topics but are mentioning plants or natural objects as an example at least twice. The 150 selected horticultural activities were classified by life-based theme, activity type, activity domain, and medium. As a result of examining horticultural activities by life-based theme, there were 150 horticultural activities (21.55%): 40 in Spring, Summer, Autumn and Winter (5.75%), 34 in Animals, Plants, and Nature (4.89%), 22 in Environment and Life (3.16%), 19 in Our Country (2.73%), and nine in Our Neighborhood (1.29%), nine in Various Countries of the World (1.29%), four in Health and Safety (0.57%), four in Living tools (0.57%), four in Transportation (0.57%), three in Kindergarten and Friends (0.43%), two in Me and My Family (0.29%; χ²=130.427, p < .001). As a result of examining horticultural activities by activity type, there were 61 free choice activities (40.67%), 80 large and small group activities (53.33%), and nine outdoor play activities (6.00%), indicating that outdoor play was the fewest activity type (χ²=54.040, p < .001). The results of analyzing horticultural activities by activity domain showed that there were 25 in conversation (16.67%), 19 in science (12.50%), 14 in art (9.33%), 14 in cooking (9.33%), 10 in fairy tales (6.00%), nine in music (6.00%), eight in language (5.33%), eight in number operation (5.33%), eight in others (5.33%), six in children's plays (4.0%), six in games (4.0%), four in body and movement (2.67%), three in stacking (2.00%), three in roles (2.00%), three in rhythm (2.00%), two in children's poems (1.33%), two in field experience (1.33%) and one in outside play (0.67%; χ²=87.600, p < .001). As a result of examining the mediums used in the horticultural activities, 46 activities (30.67%) directly used plants as the mediums, 11 activities (7.33%) used soil such as stones, gravel, and earth as the mediums instead of plants, four activities (2.67%) used dry plants such as branches and dry leaves as the mediums, and 89 activities (59.33%) used videos, photos of plants, and pictures of plants as the mediums (χ²=121.307, p < .001).