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Synthetic Studies on the Nucleophilic Addition of 1-Methyl-5-mercapto-1,2,3,4-tetrazole to Vinylsulfilimines (Vinylsulfilimine유도체에 대한 1-methyl-5-mercapto-1,2,3,4-tetrazole의 친핵성 첨가물에 관한 연구)

  • Tae-Rin Kim;So-Young Lee;Sang-Yong Pyun
    • Journal of the Korean Chemical Society
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    • v.36 no.2
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    • pp.318-323
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    • 1992
  • Following seven new nucleophilic adducts of sulfilimine compounds were prepared by the addition of 1-methyl-5-mercapto-1,2,3,4-tetrazole to vinylsulfilimine derivatives; S-Phenyl-S-2-(1-methyl-1,2,3,4-tetrazole-5-thio)-ethyl-N-p-tosylsulfilimine, S-p-tolyl-S-2-(1-methyl-1,2,3,4-tetrazole-5-thio)-ethyl-N-p-tosylsulfilimine, S-m-tolyl-S-2-(1-methyl-1,2,3,4-tetrazole-5-thio)-ethyl-N-p-tosylsulfilimine, S-p-chlorophenyl-S-2-(1-methyl-1,2,3,4-tetrazole-5-thio)-ethyl-N-p-tosylsulfilimine, S-p-bromophenyl-S-2-(1-methyl-1,2,3,4-tetrazole-5-thio)-ethyl-N-p-tosylsulfilimine, S-p-methoxyphenyl-S-2-(1-methyl-1,2,3,4-tetrazole-5-thio)-ethyl-N-p-tosylsulfilimine and S-p-nitrophenyl-S-2-(1-methyl-1,2,3,4-tetrazole-5-thio)-ethyl-N-p-tosylsulfilimine. The structures of these adducts were confirmed by elemental analyses, MP, UV, IR-and NMR-Spectra.

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Variations of pH Values and EC of Stemflow of 5 Tree Species in Chunchon Districts, Kangwon-do (강원도(江原道) 춘천시(春川市) 지역(地域)에 있어서 5개(個) 수종(樹種) 수간류(樹幹流)의 pH 및 전기전도도(電氣傳導度) 변화(變化))

  • Kim, Min Sik;Seomun, Won;Chun, Kun-Woo
    • Journal of Korean Society of Forest Science
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    • v.90 no.4
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    • pp.413-419
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    • 2001
  • This study investigated pH values and EC changes of rainfall and stemflow were investigated for 5 tree species such as Pinus koraiensis, P. densiflora, Larix leptolepis, Quercus variabilis and Q. mongolica in the Experiment Forests, College of Forest Science, Kangwon National University. The results were as follows; 1. Mean month pH values of stemflow were much lower than those of rainfall. Among tree species, pH values of the coniferous such as P. densiflora, P. koraiensis and L. leptolepis were lower those of the deciduous such as Q. mongolica and Q. variabilis. Mean month pH values of stemflow ranged from 3.23 to 5.03 (average : 4.23) for P. koraiensis, 3.57 to 5.16 (average : 4.36) for P. densiflora, 3.41 to 6.69 (average : 4.45) for L. leptolepis, 3.86 to 6.11 (average : 4.70) for Q. variabilis and 4.39 to 6.13 (average : 5.18) for Q. mongolica. 2. Mean month EC of the coniferous species was 1.5 to 2 times higher than that of the deciduous species. Mean month EC of stemflow for P. koraiensis, P. densiflora, L. leptolepis, Q. variabilis and Q. mongolica was ranged from 29.0 to $946.5{\mu}S/cm$(average : $258.5{\mu}S/cm$), 18.6 to $907.5{\mu}S/cm$(average : $220.3{\mu}S/cm$), 27.0 to $918.0{\mu}S/cm$(average : $216.2{\mu}S/cm$), 16.5 to $301.6{\mu}S/cm$(average : $125.4{\mu}S/cm$) and $25.2{\sim}225.7{\mu}S/cm$(average : $101.5{\mu}S/cm$), respectively. 3. Average pH values and EC were highly variable according to season. Average pH values appeared lower in spring than those in summer and autumn, but EC appeared higher in spring than those lower in summer and autumn. 4. According to precipitation, there was little difference in and pH values of stemflow the coniferous and the deciduous showed low pH valuess in the case of less than 50mm precipitation. However, EC of stemflow showed a difference depending on the precipitation, and EC of the coniferous and the deciduous was high more than $100{\mu}S/cm$ at the precipitation of less than 50mm.

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ANTIBACTERIAL EFFECT OF POLYPHOSPHATES ON MUTANS STREPTOCOCCI (Mutans streptococci에 대한 polyphosphate의 항균효과)

  • Kang, Kye-Sook;Choi, Yeong-Chul
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.1
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    • pp.80-91
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    • 2003
  • Mutans streptococci, especially S. mutans and S. sobrinus strongly implicated in pathogenesis of dental caries, the major cause of tooth loss in children. Use of an antibacterial agent controlling dental caries has been rationalized. The present study was performed to observe the antibacterial effect of inorganic polyphosphates (polyP) on S. mutans and S. sobrinus. S. mutans GS5 and S. sobrinus 6715 were grown in brain-heart infusion broth with or without polyP. Minimal inhibitory concentration (MIC) of polyP for S. mutans GS5 was determined to be 0.08% and that for S. sobrius 6715 was 0.17%. PolyP 15 added to the growing culture of S. mutans GS5 and S. sobrinus 6715 at their exponential phase was as effective in inhibiting the growth of S. mutans GS5 and S. sobrinus 6715 as polyP added at the very beginning of the culture. More than 85% of the cells lost their viability determined by viable cell count when polyP 15 was added to the culture of growing S. mutans GS5 at MIC, suggesting that polyP 15 has bacterial effect on the bacterium. And more than 99.9% of the cells lost their viability determined by viable cell count when polyP 15 was added to the culture of growing S. sobrinus 6715 at MIC, suggesting that polyP 15 has bacterial effect on the bacterium. Intracellular nucleotide release from S. mutans CS5 and S. sobrinus 6715 was increased in the presence of polyP 15 for 5h but was not really reversed by the addition of divalent cations like $Ca^{++}\;and\;Mg^{++}$. The majority of the cells appeared to be atypical in their shape, demonstrating accumulation of highly electron-dense granules and ghost cells. The overall results suggest that polyP have a strong bactericidal activity against S. mutans and S. sobrinus in which lysis in relation to chelation may not play the major role but unknown mechanism that possibly affects the viability of the bacterium may be involved. PolyP may be used as an agent for prevention of dental caries.

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The Effects of Inorganic Soil Amendment on Growth of Creeping Bentgrass(Agrostis palustris) in Golf Course (무기 토양개량제가 골프장 그린의 크리핑 벤트그래스 생육에 미치는 영향)

  • Lee, Jae-Pil
    • Asian Journal of Turfgrass Science
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    • v.26 no.2
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    • pp.110-115
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    • 2012
  • Soil amendments have been used to improve the physical and chemical condition of turf soil, which might optimize turfgrass growth in golf courses. This study was to investigate the effect of inorganic soil amendment (ZC) established in USGA root zone system on growth of creeping bentgrass, cv. 'Penncross' carried out from May to Dec. in 2005 at the nursery on Sinwon Golf Course. To analyze the effects of inorganic soil amendment on pH, specific gravity, infiltration rate, water content, soil hardness, root length, tiller density and dry weight were measured. pH was 6.7-6.8, specific gravity of sand (S) 100% was 1.48 heavier than the other treatments (1.28-1.38). Infiltration rate with ZC 15% + peat moss (P) 5% + S 80% and ZC 10% + S 90% was faster than S 100%. Soil hardness of S 100% was the highest. Root length of creeping bentgrass of P 10% + S 90% (8.6-12.0 cm) was the longest. Tiller density with P 5% + S 95% was more 4-7 ea than other treatments in summer season. In growing season, however, ZC 10% + P 5% + S 85% was more 2-3 ea than others. Dry weight of creeping bentgrass treated by ZC 10% + P 5% + S 85% in summer season and P 5% + S 95% in growing season were heavier than other treatments. It is recommended to combine 5-15% inorganic soil amendment and peat moss 5% with sand in order to sustain soil balance.

Effects of Planting Soil on the Soil Moisture and the Growth of Vitex rotundifolia for Green Roof (옥상녹화 식재지반이 토양수분과 순비기나무의 생육에 미치는 영향)

  • Park, Jun-Suk;Park, Je-Hea;Ju, Jin-Hee;Yoon, Yong-Han
    • Journal of the Korean Institute of Landscape Architecture
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    • v.38 no.3
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    • pp.98-106
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    • 2010
  • This study focuses on the appropriate planting soil for Vitex rotundifolia by planting soil. Different soil depth levels were achieved at 15cm and 25cm in the green roof module system that was created with woody materials for a $500{\times}500{\times}300mm$ area. The soil mixture ratio was $S_{10}$, $L_{10}$, $S_7L_3$, $S_5L_5$, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$ and $P_4P_4L_2$. This study was carried out over five months between April and September, 2006. The amount of soil moisture tends to decrease according to the planting soil. For the experimental items $S_{10}$, $S_7L_3$ and $S_5L_5$, the amount of soil moisture tends to decrease rapidly. However, for the experimental items $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$ and $P_4P_4L_2$, conditions containing perlite and peat moss, the amount of moisture tends to decrease more gradually. As a result, the use of soil-improving amending for the afforestation planting of roofs with a low level of management is need. After experimenting with the ratio of soil mixture for Vitex rotundifolia, the planting soil for experimental item $P_6P_2L_2$, $P_5P_3L_2$ and $P_4P_4L_2$ appeared excellent. For experimental item $S_{10}$, the growth of Vitex rotundifolia seemed to be weaker than that of others, because of the low levels of moisture and organic matter in the soil. For experimental item $L_{10}$, there appeared to be a low level of growth, even when the levels of moisture and organic matter were high. This may have occurred because of the low level of soil pH and the excessive amount of exchangeable cation. At the depth of 25cm, the growth of Vitex rotundifolia is vigorous overall. For experimental item at 15cm, Vitex rotundifolia was able to survive for 14 days without any rainfall and Vitex rotundifolia was better in amended soil, $P_6P_2L_2$, $P_5P_3L_2$ and $P_4P_4L_2$, than natural soil, SL.

Level of Moral Development in Pre-dental Professionals (일부 예비치과 전문인력의 도덕성 발달수준)

  • Kwag, Jung-Sook;Moon, Sang-Eun;Kim, Yun-Jeong
    • Journal of dental hygiene science
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    • v.12 no.2
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    • pp.103-108
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    • 2012
  • A study was designed to evaluate the level of moral development in 450 pre-dental professionals of 2 educational institution in Jeollabukdo, Ik san city. Data were collected through self-reported questionnaires from October 2010. The Korean version of the DIT(Defining Issues Test) was adopted to evaluate level of moral development with the score of P(%) and stage. The data were analyzed by descriptive statistics, t-test, one-way ANOVA and Pearson's correlation coefficient. The mean scores of P(%) was 43.10 in dental students and 41.21 in dental hygiene students(p=0.190). The score of stage 5A was highest which was followed by S3, S4, S6, S5B, S2. The score of stage 5B and 6 revealed sigificant difference by groups. The score of stage 6 revealed significant difference by sex in dental students(p=0.003). In dental hygiene students, it's significant difference by religion(S5B, p=0.044), birth order(S2, p=0.027) and growth area(S4, p=0.015). As for the correlations between moral development and the score of the stage, the score of P(%) was negatively correlated with the scores of stage 2, 3, 4 and 5B. On the other hand, the score of P(%) was positively correlated with the scores of stage 5A and 6. In conclusion, for enhancing dental students' moral development it is necessary a systemic ethics education and program development in curriculum.

Properties of Hydration and Strength of Sol-gol Derived Fine Particle in the System $CaO-P_2O_5-SiO_2$ (졸겔법에 의한 $CaO-P_2O_5-SiO_2$계 미세분말의 수화 및 강도특성)

  • 이형우;김정환
    • Journal of the Korean Ceramic Society
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    • v.31 no.10
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    • pp.1231-1239
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    • 1994
  • In this study, gel powder which had relatively high hydration reactivity in CaO and P2O5 rich composition of CaO-P2O5-SiO2-H2O system was prepared by sol-gel process and its hydrated specimen was manufactured. The it was investigated to appropriate calcination temperature in sol-gel process which hydrated specimen of gel powder have proven to strength and the effect of factors influenced strength in hydration process. The major product of before and after hydration reaction was hydroxyapatite, and crystalline phase of C-S-H was already formed during gelation process. After hydration reaction of pressed specimen, crystalline phase of C-S-P-H was formed. It was hydrated product of silicocarnotite (5CaO.P2O5.SiO2). Gel phases of C-S-H and C-S-P-H occured as a result of partial substitution of amorphous silica by P2O5 was formed. The strength of hydrated hardened body is developed by strong bonding and bridging between the gel phases of C-S-H or C-S-P-H and the crystalline products such as hydroxyapatite, Ca(OH)2 C-S-H and C-S-P-H. In addition, the ultrafine gel powder have an great effect on increase of hydration reaction.

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A Study on Artificiality Salivary pH and Sugar Fermentation Test of Caries Potentiality Foods (우식유발식품에 의한 인공타액내 pH 변화와 당 분해 효소 활성에 관한 연구)

  • Ji, Yun-Jeong;Park, Su-Kyung
    • Journal of dental hygiene science
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    • v.12 no.4
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    • pp.303-309
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    • 2012
  • This study was designed to provide basic data for developing a standardized caries potentiality index to help to choose snack foods with a low score on the index by investigating adolescents' snack intakes and measuring them in total saccharinity, pH, and acidogenic potential and glucosidase activity of Streptococcus mutans (S. mutans). Total 28 snack foods were selected and measured for total saccharinity (Pocket refractometer PAL-1, ATAGO) and pH with a pH meter (Mentor, Seoul, Korea). Artificial saliva was added to each sample. The experiment group was administered with S. mutans (ATCC 3692), cultured in a constant temperature incubator at $37^{\circ}C$, and measured for pH changes over five times including after 10 minutes, 30 minutes, 1 hour, and 24 hours. Each sample of 0.3 ml was added to tubes containing sucrose, galactose, or glucose to measure the glucosidase activity of S. mutans. They were then observed for glucosidase activity with colorimetry after 24 hours of culture in a constant temperature incubator at $37^{\circ}C$. The mean pH by the acidogenic potential of S. mutans was pH 5.33. The experiment group dropped in pH more than the control group due to the increasing acidogenic potential of S. mutans by glucosidase activity, recording pH 5.27 after 10 minutes, pH 5.21 after 30 minutes, pH 5.15 after 1 hour, and pH 4.80 after 24 hours. The observation results of glucosidase activity of S. mutans with colorimetry show that most of the samples were positive in orange and yellow with glucose, sucrose, and glactose recording activity of 78.58%, 75%, and 71.42%, respectively.

Effect of Nutrient Solution Strength on Growth of Phalaenopsis in an Ebb and Flow System (Ebb and Flow 시스템을 이용한 호접란 수경재배시 생장에 미치는 배양액 농도의 영향)

  • An, Dong-Choon;Park, Seon-Hye;Been, Chul-Gu;Jeong, Byoung-Ryong
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.199-204
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    • 2007
  • This experiment was conducted to investigate the effect of nutrient solution strength on the growth of miniature multiflora P. amabilis and P. Taisuco Red Jewel at two growth stages during four months cultivation in an ebb and flow system. Early stage plants gained the biggest leaf length and width when nutrient solution strength was EC 0.5 or $1.0dS{\cdot}m^{-1}$ in P. amabilis, and EC $1.5dS{\cdot}m^{-1}$ in P. Taisuco Red Jewel. Root length and weight were the greatest when nutrient solution strength was EC $0.5dS{\cdot}m^{-1}$ in both cultivars and this trend was also found in middle stage plants. Fresh and dry weights of leaves increased as nutrient solution strength was elevated and were the greatest when nutrient solution strength was EC $1.5dS{\cdot}m^{-1}$ in P. amabilis. On the contrary elevation of nutrient solution strength decreased fresh weight of roots, being the greatest when nutrient solution strength was EC $0.5dS{\cdot}m^{-1}$. This trend was also found in P. Taisuco Red Jewel and P. amabilis at middle growth stage. The T/R ratio and chlorophyll content increased with nutrient solution strength and were the greatest regardless of cultivar and growth stage when nutrient strength was EC $1.5dS{\cdot}m^{-1}$.

Properties of Recombinant Derivatives of pJY501, A Multi-copy Streptomyces plasmid (Multi-copy Streptomyces 플라스미드, pJY501의 재조합 유도체의 특성)

  • 염도영;공인수;유주현
    • Microbiology and Biotechnology Letters
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    • v.18 no.1
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    • pp.94-97
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    • 1990
  • The restriction cleavage map of multi-copy recombinant plasmid, pJY502 (5.5 kb), carrying the thiostrepton resistance gene (tsr) was determined. Comparison of the restriction pattern with that of Streptomyces plasmids previously demonstrated that pJY502 was novel. The plasmid pJY502 had a broad host range in Streptomyces and contained single BgtII site for cloning purpose. Transformation frequency of pJY502 was $2.2 \times 10^5$ in S. lividans. E. coti-Streptomyces bifunctional plasmid, pJY504, was also constructed.

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