• 제목/요약/키워드: p53-p21 system

검색결과 126건 처리시간 0.035초

ITGB2 유전자 다형성과 결핵 사이의 연관성 연구 (Association between ITGB2 Genetic Polymorphisms and Tuberculosis)

  • 진현석;이상인;박상정
    • 대한임상검사과학회지
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    • 제50권2호
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    • pp.118-125
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    • 2018
  • 결핵은 본질적으로 MTB에 의해 발생하는 감염성 질환이지만 발병의 과정에는 숙주의 면역계와 연관성 있는 하는 유전자가 관여한다. ITGB2 유전자는 인테그린 beta 2 chain인 CD18 단백질을 암호화 하고 있는 유전자로 염색체 21번에 존재하고 있다. 인테그린 beat 2 chain은 백혈구에서 발현하는 인테그린으로 백혈구의 성숙 및 부착에 매우 중요한 역할을 수행하는 단백질이다. ITGB2는 결핵 발병에서 MTB의 탐식과 백혈구의 집합에도 중요한 역할을 수행한다고 알려졌다. 따라서 이번 연구는 한국인의 유전체 데이터를 활용하여 결핵 발생 환자들과 정상 대조군 사이에서 ITGB2의 유전적 다형성의 빈도에 통계적으로 유의한 차이가 존재하는지를 알아보고자 하였다. 그 결과 10개의 SNP에서 유의한 상관관계를 확인할 수 있었다. 가장 유의성 있는 SNP는 rs113421921 였다 (OR=0.69, CI: 0.53~0.90, $P=5.8{\times}10^{-3}$). 또한 rs173098의 경우는 전사 보조인자인 p300이 결합할 가능성이 있는 염기서열이 존재하여 유전적 다형성에 따라 ITGB2 유전자 발현에 영향을 미칠 수 있음을 확인할 수 있었다. 이러한 결과는 결핵의 발병 기전이 백혈구 집합이나 부착과 같은 숙주의 면역 기능과 관련된 다양한 유전적 요인에 의해 영향을 받을 수 있음을 시사한다. 이 연구결과는 결핵의 발병에 숙주 면역계의 유전자들이 영향을 줄 수 있다고 볼 수 있다. 이러한 결과들을 통해 MTB 감염에 대해 각 사람들 별로 감염의 진행과정과 결과에 차이를 가져다 주는 유전적 배경에 대한 이해에 기반을 제공할 것으로 기대한다.

농촌 환경ㆍ건강 지표의 변화 추이(2001~2010년) -농촌 거주 농업종사자와 비농업종사자의 비교- (Changes in Rural Health and Environmental Factors (2001-2010) A Comparison between Agricultural and Nonagricultural Groups)

  • 이승교;황정임;원향례
    • 한국지역사회생활과학회지
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    • 제25권1호
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    • pp.99-111
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    • 2014
  • To verify welfare needs, rural health and environmental factors were compared between agricultural and nonagricultural groups over the past 10 years. Based on a survey of rural life by the Rural Development Administration, whose sample included agricultural (72.8%) and nonagricultural (27.2%) groups, all factors were analyzed using SAS ( 9.3). For rural home heating, the use of oil boilers (77.2%-78.0%) decreased (53.0%-53.7%) over the same period, whereas that of electric boilers increased (21.9% and 13.5% for agricultural and nonagricultural groups, respectively). The joint water- supply increased, and the use of flush toilets (52.4% to 84.5% in the agricultural group and 64.6% to 81.4% in the nonagricultural group) and hot-water bathing was higher in the agricultural group (79% to 92.6%) than in the nonagricultural group (72.2% to 87.6%) at the p<0.001 level. Incineration accounted for the largest portion of household waste until 2006, when it was replaced by other treatment methods such as the pay-per-treatment option (44.4% and 68.3% in the agricultural and nonagricultural groups, respectively) (p<0.001). Garbage disposal in 2001 was mainly burial or animal feed, but separate collection increased in 2010 (42.2% and 64.3% in the agricultural and nonagricultural groups, respectively) (p<0.001). The self-perception of health was lower in the agricultural group than in the nonagricultural group (2.96 to 2.74 on a five-point scale in the agricultural group and 3.07 to 2.98 in the nonagricultural group). Drinking decreased less in the agricultural group (criteria: one point once a week,; 1.48 to 1.20) than in the nonagricultural group (1.13 to 0.80) at p<0.001, and a similar pattern was found for smoking (p<0.001). Health screening decreased in the last 10 years (scoring based one point/1-2 times per year,: 0.94 to 0.64 in the agricultural group and 1.08 to 0.69 in the nonagricultural group;p<0.01). These results indicate that various health and environmental factors were less favorable to farmers except for bathing in comparison to nonagricultural living in the same area. This suggests that people in agriculture are less likely to live and work in favorable environments than those outside the sector and thus indicates a need for more attention to the national health welfare system for farmers for systematic realization.

균형성과표를 활용한 전자의무기록시스템의 성과측정 모형개발 (Development of the Performance Measurement Model of Electronic Medical Record System - Focused on Balanced Score Card -)

  • 이경희;김영훈;부유경
    • 한국병원경영학회지
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    • 제21권4호
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    • pp.1-12
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    • 2016
  • The purpose of this study are suggest to performance measurement model of Electronic Medical Record(EMR) and Key Performance Index(KPI). For data collection, 665 questionnaires were distributed to medical record administrators and insurance reviewers at 31 hospitals, and 580 questionnaires were collected(collection rate: 87.2%). Regarding methodology, Critical Success Factor(CSF) and index of the information system were derived based on previous studies, and these were set as performance measurement factors of EMR system. The performance measurement factors were constructed by perspective using BSC, and analysis on causal relationship between factors was conducted. A model of causal relationship was established, and performance measurement model of EMR system was proposed through model validation. Analysis on causal relationship between performance management factors revealed that utility cognition of the learning & growth perspective factor had causal relationship with job efficiency(${\beta}=0.20$) and decision support(${\beta}=0.66$) of the internal process perspective factors, and security had causal relationship with system satisfaction(${\beta}=0.31$) of the customer perspective factor. System quality had causal relationship with job efficiency(${\beta}=0.66$) and decision support(${\beta}=0.76$) of the internal process perspective factors, all of which were statistically significant(P<0.01). Job efficiency of the internal process perspective had causal relationship with system satisfaction(${\beta}=0.43$), and decision support had causal relationship with decision support satisfaction(${\beta}=0.91$) and job satisfaction (${\beta}=0.74$), all of which were statistically significant(P<0.01). System satisfaction of the customer perspective had causal relationship with job satisfaction(${\beta}=0.12$), job satisfaction had causal relationship with cost reduction(${\beta}=0.53$) of the financial perspective, and decision support satisfaction had causal relationship with productivity improvement(${\beta}=0.40$)of the financial perspective(P<0.01). Also, cost reduction of the financial perspective had causal relationship with productivity improvement(${\beta}=0.37$), all which were statistically significant(P<0.05). Suitability index verification of the performance measurement model whose causal relationship was found to be statistically significant revealed that $X^2/df=2.875$, RMR=0.036, GFI=0.831, AGFI=0.810, CFI=0.887, NFI=0.838, IFI=0.888, RMSEA=0.057, PNFI=0.781, and PCFI=0.827, all of which were in suitable levels. In conclusion, the performance measurement indices of EMR system include utility cognition, security, and system quality of the learning & growth perspective, decision support and job efficiency of the internal process perspective, system satisfaction, decision support satisfaction, and job satisfaction of the customer perspective, and productivity improvement and cost reduction of the financial perspective. In this study, it is expected that the performance measurement indices and model of EMR system which are suggested by the author, will be a measurement tool available for system performance measurement of EMR system in medical institutions.

CT-Guided Percutaneous Transthoracic Needle Biopsy Using the Additional Laser Guidance System by a Pulmonologist with 2 Years of Experience in CT-Guided Percutaneous Transthoracic Needle Biopsy

  • Jeon, Min-Cheol;Kim, Ju Ock;Jung, Sung Soo;Park, Hee Sun;Lee, Jeong Eun;Moon, Jae Young;Chung, Chae Uk;Kang, Da Hyun;Park, Dong Il
    • Tuberculosis and Respiratory Diseases
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    • 제81권4호
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    • pp.330-338
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    • 2018
  • Background: We developed an additional laser guidance system to improve the efficacy and safety of conventional computed tomography (CT)-guided percutaneous transthoracic needle biopsy (PTNB), and we conducted this study to evaluate the efficacy and safety of our system. Methods: We retrospectively analyzed the medical records of 244 patients who underwent CT-guided PTNB using our additional laser guidance system from July 1, 2015, to January 20, 2016. Results: There were nine false-negative results among the 238 total cases. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy of our system for diagnosing malignancy were 94.4% (152/161), 100% (77/77), 100% (152/152), 89.5% (77/86), and 96.2% (229/238), respectively. The results of univariate analysis showed that the risk factors for a false-negative result were male sex (p=0.029), a final diagnosis of malignancy (p=0.033), a lesion in the lower lobe (p=0.035), shorter distance from the skin to the target lesion (p=0.003), and shorter distance from the pleura to the target lesion (p=0.006). The overall complication rate was 30.5% (74/243). Pneumothorax, hemoptysis, and hemothorax occurred in 21.8% (53/243), 9.1% (22/243), and 1.6% (4/243) of cases, respectively. Conclusion: The additional laser guidance system might be a highly economical and efficient method to improve the diagnostic efficacy and safety of conventional CT-guided PTNB even if performed by inexperienced pulmonologists.

침상각도 상승 교육에 대한 중환자실 간호사의 지식, 인식 및 수행 정도 (The Effect of Backrest Elevation Education on ICU Nurse's Knowledge, Perception and Performance)

  • 이현심;박영우;김정연;이은숙;박애순;한아름;김은아;이호선;고신옥
    • 임상간호연구
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    • 제14권3호
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    • pp.117-128
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    • 2008
  • Purpose: This study was conducted to examine the differences of knowledge, perception, and performance between the points of time before and after ICU nurses had the backrest elevation education. Method: The study subjects were 58 nurses at a medical and surgical ICU of one general Y hospital located in Seoul. They received the education, including backrest elevation guideline and related education materials. Data were collected from May 11 throughout August 12, 2007 with a structured questionnaire. Results: 1) There were significant differences in the mean scores of knowledge(2.21 at pre-education, 5.24 at post-education), perception(36.96 at pre-education, 53.36 at post-education), and performance(32.08 at pre-education, 43.51 at post-education), 2) There was a significant correlation between nurse's perception and performance (p=.000). 3) The nurse's perception regarding the importance of the back rest elevation education was significantly effective on their performance (p=.000). Conclusion: The backrest elevation education would contribute to improve ICU nurse's knowledge, perception and performance. The more the nurses would consider the importance of this education, the better they would perform the nursing intervention of backrest elevation.

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Effects of Green Tea Catechin on Mixed Function Oxidase System and Antioxidative Defense System in Rat Lung Exposed to Microwave

  • Kim, Mi-Ji;Rhee, Soon-Jae
    • Preventive Nutrition and Food Science
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    • 제9권1호
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    • pp.53-57
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    • 2004
  • The purpose of this study was to investigate the effects of green tea catechin on mixed function oxidase system (MFO), lipofuscin contents, carbonyl value, oxidative damage and the antioxidative defense system in lung of microwave exposed rats. Experimental groups were divided to normal group and microwave exposed group. The microwave exposed groups were subdivided into three groups: catechin free diet (MW-0C) group, 0.25% catechin (MW-0.25C) group and 0.5 % catechin (MW-0.5C) group according to the levels of dietary catechin supplementation. The rats were irradiated with microwave at frequency of 2.45 GHz for 15 min. Experimental animals were sacrificed at 6th day after microwave irradiation. The contents of cytochrome P$_{450}$ contents in MW-0C group was increased to 95% , compared with normal group. MW-0.25C and MW-0.5C groups were reduced to 16% and 31%, respectively, compared with MW-0C group. The activity of NADPH-cytochrome P$_{450}$ reductase in MW-0C group was increased to 44%, compared with normal group. MW-0.25C and MW-0.5C groups were reduced to 12% and 17%, respectively, compared with MW-0C group. The activity of superoxide dismutase (SOD) in MW-0C group was decreased to 21 %, compared with normal group. MW-0.25C and MW-0.5C group were significantly (p < 0.05) increased, compared with MW-0C group. The activity of glutathione peroxidase (GSHpx) in MW-0C group was significantly decreased, compared with normal group. MW-0.25C and MW-0.5C groups were recovered to the level of normal group. The thiobarbituric acid reactive substances (TBARS) content in MW-0C group was increased to 34 %, compared with normal group. Catechin supplementation groups were maintained the level of normal group. The levels of caybonyl value in MW-0C group was increased to 21 %, compared with normal group. MW-0.25C and MW-0.5C groups were reduced to 14% and 12%, respectively, compared with MW-0C group. The lipofuscin contents in MW-0C group were increased to 23.4 %, compared with normal group. That of MW-0.5C group was significantly reduced, compared with MW-0C group. In conclusion, MFO system was activated and the formation of oxidized protein, lipofuscin was increased and antioxidative defense system was weakened of lung tissue in microwave exposed rats, thus oxidative damage was increased. But it was rapidly recovered to normal level by green tea catechin supplementation.n.

중년여성의 갱년기 증상과 건강요구에 대한 기초조사 (A study on menopausal symptoms and health needs among middle aged women.)

  • 차영남;장효순;김금자;한혜실;임혜경;정영해
    • 대한간호
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    • 제34권4호
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    • pp.70-82
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    • 1995
  • During the middle age of a woman's life cycle. several health changes and problems occur. Therefore. middle aged women must manage their health and maintain quality life by coping with bodily changes. However. today there is not enough research and health programs for middle aged women. Data from the study will be used for health promotion program development of middle aged women. Data was collected from January 21 to 24. 1995 by telephone interview. Four hundred middle aged women between 40 and 59 years old and living in Chon Ju City were interviewed. The results of this study were as follow: 1. Frequency rate of menopausal symptoms was 38.4%. The most serious menopausal symptom was psychosomatic symptom. Mean score orders of experienced symptoms were back pain, joint pain(1.80). nervousness(1.80). general weakness(1.67). 2. The most important problems as perceived by the clients were children(45.8%), health (24.0%) and economics(7.8%). The most serious health problems were concerning the muscle-skeletal system(45%) such as arthritis. spinal disk problems and osteoporosis. Adult diseases04.S%) such .as hypertension and diabetes were also health concerns. Health management activities reported were exercise(22.5%), social activity02%) and inactivity(53%)' 33% of clients were interested in health groups and they wanted a program of health education, exercise and social activity to be provided. 3. General characteristic variables were significantly related to the level of menopausal symptoms as follows: age(t=-2.06, p=0.040), status of marriage(t=-3.56, p=0.000), educational level (F=4.35. p=0.05) and menopausal status(t=4.37, p=0.000).

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흰쥐에서 Cyclosporine의 약동학적 지표에 대한 Nicardipine의 영향 (Effect of Nicardipine on the Pharmacokinetic Parameters of Cyclosporine in Rat)

  • 김희규;강주섭;이창호;신인철
    • Biomolecules & Therapeutics
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    • 제6권4호
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    • pp.389-394
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    • 1998
  • Cyclosporine (CsA) is a major immunosuppressive drug used widely to prevent organ allograft rejection. fits potential organotoxicity by prolonged use is known to cause both direct tissue damage and indirect pharmacokinetic interactions with other drugs. This study was performed to determine the effect of nicardipine (NCP) on the pharmacokinetic parameters of CsA in Sprague-Dawley rats. Each rat was administered with CsA in saline-treated group or in NCP-treated group which was pretreated with NCP (5 mg/kg/12 hours, i.p.) for 6 days. The plasma CsA concentration were analyzed by reversed HPLC: UV system at 0.5, 1, 2, 4, 6, and 8 hours after bolus injection of CsA (10 mg/kg). Pharmacokinetic parameters (mean$\pm$ SD, n=7) such as initial plasma concentration (C(0)), mean residence time (MRT), steady-state volume of distribution (Vdss), terminal half-life (t$\frac{1}{2}$($\beta$)) and plasma clearance (CLp) of CsA in each groups (saline-group vs NCP-group) were determined as follows: C(0) (5.66$\pm$ 1.98 vs 17.98$\pm$2.36, p<0.01); Vdss (2.68$\pm$ 1.6 vs 0.94 $\pm$ 0.25, p<0.01); CLp (0.53 $\pm$0.18 vs 0.21 $\pm$0.06, p<0.01). Therefore, Our results indicate that nicardipine significantly affects the pharmacokinetic parameters of cyclosporme, especially C(0), Vdss, and CLp in NCP-treated group. We suggest that the significant pharmacokinetic interaction between cyclosporine and nicardipine should be considered and cyclosporine level should be closely monitored and dosage reduction made as necessary in clinical situation that was coadministered with CsA and NCP.

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토양(土壤)의 물리적(物理的) 조건(條件)이 은수원사시나무(Populus alba × P. glandulosa)의 생장(生長) 및 근계발달(根系發達)에 미치는 영향(影響) - 용적중(容積重)을 중심(中心)으로 - (Influence of Soil Bulk Density on Growth and Root Development of Populus alba × P. glandulosa)

  • 민일식;이수욱
    • 한국산림과학회지
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    • 제66권1호
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    • pp.45-53
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    • 1984
  • 본(本) 연구(硏究)는 경사부위별(傾斜部位別) 토양별(土梁別)로 토양(土壤)의 물리적(物理的) 성질(性質) 중(中) 용적량(容積量)과 공극률(孔隙率)이 은수원사시나무의 생장(生長)과 근계발달(根系發達)에 미치는 영향(影響)을 분석(分析)한 것으로서 그 결과(結果)를 요약(要約)하면 다음과 같다. 1) 용적중(容積重)과 공극률(空隙率)은 경사부위(傾斜部位)와 밀접(密接)한 관계를 가지고 있으며, 토심(土深)에 따라서 유의적(有意的)인 차이(差異)를 갖는다. 2) 용적중(容積重)은 하부(下部)에서 평균(平均) $1.17g/cm^3$(1.05~1.40), 상부(上部)에서 평균(平均) $1.43g/cm^3$(1.36~1.60)이었으며, 공극률(空隙率)은 하부(下部)에서 평균(平均) 52.65%(55.05~45.50), 상부(上部)에서 평균(平均) 41.20%(43.81~37.21)이었다. 3) 용적중(容積重)은 수고(樹高), 흉고직경(胸高直徑), Total biomass 및 임목(林木)의 각(各) 부위별(部位別) Biomass와 회귀분석결과(回歸分析結果) 부(負)의 상관(相關)을 나타내었다. 4)은수원사시나무의 근계(根系)는 천근성(淺根性)으로서 주로 Al속(屬)에 분포(分布)하였으며, 한계용적중(限界容積重)은 $1.40g/cm^3$이었다.

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치과용 국소마취 주사시 압력조절형 주사장치 사용의 효과에 대한 연구 (Study on Effect of Pressure-Controlled Injection System in Dental Local Anesthesia)

  • 윤필영;김영균
    • 대한치과마취과학회지
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    • 제6권2호
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    • pp.98-102
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    • 2006
  • The purpose of this study was to evaluate the difference on pain intensity and discomfort between pressure-controlled injection system and conventional syringe injection technique from the patients undergoing oral and maxillofacial surgery. In a prospective, randomized, case-controlled study, 60 patients were divided into two groups (n=30 in each). In experimental group, pressure-controlled injection system was applied. In control group, conventional syringe injection system was applied. Pain rating score (PRS) and visual analogue scale (VAS) were assessed. The average of VAS in experimental group ($16.67{\pm}15.07$) was smaller than that of control group ($25.63{\pm}22.21$), though there were no significant differences (P=0.072). In PRS, fifteen patients (50.0%) of experimental group answered that they experienced mild pain. However sixteen patients (53.3%) of control group answered that they experienced intermediate pain. From the results, pressure-controlled injection system may be an effective method to reduce pain during the dental local anesthetic procedure.

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