• 한국응용곤충학회지
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• 제35권1호
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• pp.85-90
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• 1996

국내에서 분리된 Bacillus thuringiensis NT0423은 나비목과 파리목 곤충에 독성을 보이는 130 kDa의 전형적인 다이아몬드형 내독소 단백질을 생성한다. 이 균주의 파리목에 대한 독성을 강화하기 위하여, B. thuringeinsis NT0423에 모기 유충에 강한 독성을 보이는 B. thuringiensis subsp. morrisoni PG-14의 cryVID유전자를 가지고 있는 pCG10 플라스미드를 electroporation 방법을 이용하여 형질전환하였다. 형질전환체인 B. thuringiensis PT1227내에서 cryIVD와 숙주가 생성하는 원래의 다이아몬드형 130kDa 내독소 단백질 유전자는 그 자신의 형태로 잘 발현되었다. 형질전환체의 모기 유충에 대한 독성은 원래 숙주의 내 독소 단백질과ㅏ 도입된 CryIVD의 상승효과에 의해 현저히 증가하였다.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2005년도 제5회 발생공학 국제심포지엄 및 학술대회
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• pp.130-130
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• 2005

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2005년도 제5회 발생공학 국제심포지엄 및 학술대회
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• pp.130-130
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• 2005

• Asian-Australasian Journal of Animal Sciences
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• 제25권11호
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• pp.1559-1567
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• 2012

The objective of this study was to evaluate ruminal degradability and intestinal digestibility of crude protein (CP) and amino acids (AA) in hempseed cake (HC) that were moist heat treated at different temperatures. Samples of cold-pressed HC were autoclaved for 30 min at 110, 120 or $130^{\circ}C$, and a sample of untreated HC was used as the control. Ruminal degradability of CP was estimated, using the in situ Dacron bag technique; intestinal CP digestibility was estimated for the 16 h in situ residue using a three-step in vitro procedure. AA content was determined for the HC samples (heat treated and untreated) of the intact feed, the 16 h in situ residue and the residue after the three-step procedure. There was a linear increase in RUP (p = 0.001) and intestinal digestibility of RUP (p = 0.003) with increasing temperature during heat treatment. The $130^{\circ}C$ treatment increased RUP from 259 to 629 g/kg CP, while intestinal digestibility increased from 176 to 730 g/kg RUP, compared to the control. Hence, the intestinal available dietary CP increased more than eight times. Increasing temperatures during heat treatment resulted in linear decreases in ruminal degradability of total AA (p = 0.006) and individual AA (p<0.05) and an increase in intestinal digestibility that could be explained both by a linear and a quadratic model for total AA and most individual AA (p<0.05). The $130^{\circ}C$ treatment decreased ruminal degradability of total AA from 837 to 471 g/kg, while intestinal digestibility increased from 267 to 813 g/kg of rumen undegradable AA, compared with the control. There were differences between ruminal AA degradability and between intestinal AA digestibility within all individual HC treatments (p<0.001). It is concluded that moist heat treatment at $130^{\circ}C$ did not overprotect the CP of HC and could be used to shift the site of CP and AA digestion from the rumen to the small intestine. This may increase the value of HC as a protein supplement for ruminants.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2008년도 추계학술대회논문집
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• pp.129-130
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• 2008

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2005년도 2005 Annual Meeting & International Symposium
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• pp.129-130
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• 2005

• 충청수학회지
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• 제31권1호
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• pp.103-130
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• 2018

In the paper we establish some new results depending on the comparative growth properties of composite entire and meromorphic functions using relative $_pL^*-order$, relative $_pL^*-lower$ order and differential monomials, differential polynomials generated by one of the factors.

• 한국산학기술학회논문지
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• 제21권5호
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• pp.121-130
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• 2020

간호 대학생의 실습 전, 후 임상실습 스트레스, 수면의 질, 주간 졸림증 차이와 관계를 확인하는데 목적이 있다. 연구대상은 간호학과 3학년 학생 130명이다. 연구결과 연구 대상자의 임상실습 스트레스는 총점 5점 만점 중 평균점수 3.15점으로 나타났다. 임상실습 스트레스의 외부조정 요인 중 과제물에 대한 점수가 3.64로 가장 높았고, 성별(t=-3.88, p<.001), 주관적 건강상태(F=5.78, p=.004), 거주형태(F=3.20, p=.026)에 유의한 차이가 있는 것으로 나타났다. 간호대학생은 수면의 질이 낮고(실습 전 80.0%, 실습 후 70.0%), 주간 졸림증이 있는 대상이(실습 전 26.9%, 실습 후 42.3%) 일반 대학생에 비해 많음을 확인 할 수 있었다. 임상실습 전, 후 수면의 질은(χ²=4.06, p=.004), 주간 졸림증은(χ²=6.08, p=.009)로 유의한 차이가 있었다. 또한 주간 졸림증과 수면의 질은 음의 상관관계(r=-.259, p<0.01)가 있는 것으로 나타났다. 따라서 간호 대학생들의 신체적 건강 및 정신적 건강을 위하여 임상실습 스트레스를 줄이고 수면의 질을 높일 수 있는 전략 개발이 필요하며, 변화하는 간호 교육현장에서 임상실습 적응에 도움이 되도록 하여 추후 간호의 질을 향상시킬 수 있도록 해야겠다.

• 국제학술발표논문집
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• The 1th International Conference on Construction Engineering and Project Management
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• pp.974-979
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• 2005

Effective communication flow is a fundamental for the success of any construction projects. The key role of communications among project parties is even more critical in the case of Design-Build (DB) projects. Previous research has shown that these projects have an environment where integration between different project team members and schedule constraints increase channels of communication, and require faster communication flows. This paper summarizes the findings from research analyzing organizational structures and communication flow of the Texas State Highway 130 (SH130) project. Awarded in 2002, the SH130 project, totaling $1.3 billion USD, constitutes the "pilot" application for the DB approach in the state of Texas. A set of observations pertaining to team organization and communication issues in the design-build environment is included.

• 미생물학회지
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• 제15권3호
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• pp.123-130
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• 1977

In previous paper, it was reported that antibiotic substance such as tetracycline and streptomycin were produced by S'. albus subsp. and S'. globosus. And increase of mycelial growth of two strains, antibiotic production, and changes of pH range are extended to approximately 110-130 hrs in fermenting medium, there-after they decreased with culture period exception of pH range. Two Streptomyces spp. required commonly 4-5% starch as carbon sources and 1.5-2.0% soybean meal as nitrogen sources. However, 0.005-0.01M potassium phosphate dibasic, calcium carbonate (6mg/ml in S.albus subsp. and 2mg/ml in S. globosus), 0.01-0.03M, magnesium sulgate and 0.01M ferric chloride showed as optimal concentration for the growth of 2 strains. Mineral compoments such as zinc, manganese, cobalt, sodium and copper at the level of 10/sup -4/ -10/sup -6/M were observed. Especially, zinc ion showed toxicity to the growth of 2 strains at 0.005M. In relation with pH, there is a little difference in mycelial growth with cultural initial pH.