• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1894-1901
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• 2015

A novel sulfatase gene, ary423 (1,536 bp ORF), encoding a protein of 511 amino acids with a calculated molecular mass of 56 kDa, was identified from Flammeovirga pacifica, which was isolated from deep-sea sediments of west Pacific Ocean. Amino acid sequence analysis revealed that Ary423 possessed a conserved C-X-A-X-R motif, which was recognized as the sulfatase signature. Phylogenetic analysis suggested that Ary423 belonged to arylsulfatases. After heterologous expression in Escherichia coli cells, the recombinant Ary423 was purified with a Ni⁺ affinity column, and was shown to be highly active at a broad range of temperatures from 30° to 70℃, with maximum activity at 40℃. Furthermore, recombinant Ary423 retained more than 70% and 40% of its maximum activity after 12 h of incubation at 50℃ and 60℃, respectively, exhibiting good thermostability at high temperatures. The optimal pH for Ary423 was determined to be 8.0 and the activity of Ary423 could be slightly enhanced by Mg²⁺. The recombinant enzyme could hydrolyze sulfate ester bonds in p-nitrophenyl sulfate (NPS) and Asparagus crude polysaccharides with a specific activity of 64.8 U/mg and 25.4 U/mg, respectively. These favorable properties could make Ary423 attractive for application in the desulfating process of agar production.

• East Asian mathematical journal
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• v.15 no.2
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• pp.153-163
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• 1999

We study how the $p_n$-sequence of a universal algebra determine the structure of the algebra. Regarding term equivalent algebras as the same algebras, we consider the problem when the algebras are groupoids.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.1C
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• pp.6-11
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• 2008

From a periodic sequence, we can obtain new sequences with a longer period by r-symbol insertion to each period. In this paper we review previous results on the linear complexity of periodic sequences obtained by r-symbol insertion. We derive the distribution of the linear complexity of 1-symbol insertion sequences obtained from m-sequences over GF(p), and prove some relationship between their linear complexity and the insertion position. Then, we analyze the k-error linear complexity of the 1-symbol insertion sequences from binary m-sequences.

• Journal of Korea Multimedia Society
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• v.19 no.8
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• pp.1225-1235
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• 2016

This paper presents a method for computer-assisted slide presentation using vision-based gesture recognition. The proposed method consists of a sequence of steps, first detecting a hand in the scene of projector beam, then estimating the smooth trajectory of a hand or a pointing finger using Kalman Filter, and finally interfacing to an application system. Additional slide navigation control includes moving back and forth the pages of the presentation. The proposed method is to help speakers for an effective presentation with natural improved interaction with the computer. In particular, the proposed method of using finger pointing is believed to be more effective than using a laser pointer since the hand, the pointing or finger are more visible and thus can better grab the attention of the audience.