• Journal of Preventive Medicine and Public Health
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• 제18권1호
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• pp.51-57
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• 1985

To attempt to measure the effect of domestic product P.H.A. kit 'Hepa-S' after completion of 'Hepa-Vax' vaccination schedule, P.H.A. test and R.I.A. test on the 330 healthy adults were carried out. The results obtained were as follow ; 1. The positive anti HBs rate after completion of 'Hepa-Vax' vaccination were; in P.H.A. test with domestic product P.H.A. kit 81.2%, in P.H.A. test with foreign product P.H.A. kit 82.7%, and in R.I.A. test 95.8% 2. Using the result of R.I.A. test as the standard, sensitivity of P.H.A. test with domestic product P.H.A. kit was 84.8% and specificity was 100.0% 3. Using the result of R.I.A. test as standard, sensitivity of P.H.A. test with foreign P.H.A. kit was 86.4% and specificity was 100.0%. 4. The concordance rate of P.H.A. test with domestic product and foreign product kit was 98.5%. On the result of this study, there was no significant difference in the validity between the domestic product P.H.A. kit 'Hepa-S' and the foreign P.H.A. kit $'Hebsgencell^{TM}'$. So that it is recommendable to use domestic product P.H.A. kit instead of foreign product P.H.A. kit.

• 동아시아식생활학회지
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• 제19권1호
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• pp.78-88
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• 2009

The mechanical characteristics (texture and color) and consumer preference of Doraji Yanggengs using different pre-treatments and added levels of Doraji were presently evaluated. Three kinds of Doraji Yanggengs were made with differing amounts of Doraji powder (DPY; 30, 50, or 70 g), ground raw Doraji (GRDY; 100, 200, or 300 g), and Doraji juice (DJY; 100, 200, or 300 g). Texture characteristics (hardness, adhesiveness, springiness, chewiness, gumminess, cohesiveness) and color values (lightness, redness, yellowness) were measured three times in three experiments conducted with each formulation. Preference was measured with a nine-point hedonic scale for Yanggengs once every three experiments. Consumer preference was measured with color, flavor, taste, appearance, texture, and overall acceptability. Significant differences according to the amount of Doraji powder were evident with DPY for hardness (p<0.001), adhesiveness (p<0.05), and cohesiveness (p<0.001). Hardness (p<0.001), adhesiveness (p<0.001), chewiness (p<0.01), and gumminess (p<0.01) of 100 g GRDY displayed higher scores than 200 g and 300 g GRDY, but displayed significantly lower scores in cohesiveness (p<0.01). Hardness (p<0.001), adhesiveness (p<0.001), chewiness (p<0.001), gumminess (p<0.01), and cohesiveness (p<0.05) of DJY showed significant differences according to amount of Doraji juice. The highest score in a preference test among DPYs was evident for 30 g DPY. There were no significant differences in preference among GRDYs and DJYs. Color, flavor, taste, appearance, texture, and overall acceptability were significantly (p<0.01) correlated in all Yanggengs. DJY was preferred more than DPY and GRDY. Women in their forties and fifties preferred Yanggeng more when compared to women in their twenties and thirties.

• 한국진공학회지
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• 제15권4호
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• pp.360-366
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• 2006

0,25 ML의 산소원자가 흡착하여 이루는 O/Pd(100)-p($2{\times}2$) 표면의 원자구조를 밀도범함수 이론 계산을 통해 연구하였다, Pd(100) 표면의 fourfold hollow 위치가 가장 안정된 산소 흡착위치로 밝혀졌고 O-Pd 결합거리는 $2,15{\AA}$으로 계산되었다. 산소 흡착에 의해 Pd(100) 표면의 첫 번째 층간격 ($d_{12}$) 은 +0,8%의 팽창을 보이는데 이 계산결과는 +3,6%의 팽창을 보고 한 LEED 실험 결과와 차이를 보인다. 차이의 원인으로 시료 표변에 수소 불순물이 존재하였을 가능성을 고려하여 계산한 결과, O/Pd(100)-p($2{\times}2$)에 수소원자가 흡착할 때 $d_{12}$의 팽창이 유발됨을 확인하였다. 수소 흡착량에 따른 구조변화를 분석하여 LEED 시료 표변에 잔존할 것으로 예상되는 수소의 양을 약 0,3 ML로 추정하였다.

• 한국미생물·생명공학회지
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• 제26권6호
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• pp.562-565
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• 1998

Nisin-producing and nisin resistant L. lactis subsp. lactis ATCC7962 harbored six plasmids. To find a plasmid containing a nisin resistant gene, these plasmids were transformed into L lactis LM0230 of plasmid-free and nisin sensitive strain. After screening on nisin selection media containing nisin (150 $\mu\textrm{g}$/$m\ell$), several nisin resistant transformants were obtained and the level of nisin resistance was very similar to that of wild type L lactis subsp. lactis ATCC7962. A 26.5 kb plasmid, named as pCS100, which confers resistance to nisin, was identified in transformants. The pCS100 was digested with EcoRI and Southern blot hybridization was done with nisI probe to localize the nisin resistant gene. A 4 kb EcoRI fragment showed a strong positive signal, and it was cloned into pBluescript for the potential selection marker.

• 한국양식학회지
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• 제19권4호
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• pp.261-266
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• 2006

본 연구는 쉬리(Coreoleuciscus splendidus)를 대상으로 사료내 색소원으로서 paprika 및 spirulina 첨가와 원료 각각의 첨가량을 달리하여 쉬리의 생존율과 색택개선에 미치는 영향을 조사하였다. 실험어는 평균체중 약 4.4 g인 쉬리를 사용하였으며, 총 8개(Control, P1, P2, P3, S1, S2, S3 및 P2+S2)의 실험 사료를 설계하였다. 사료 P1, P2 및 P3는 paprika를 각각 2%, 5% 및 10%를 첨가하였으며, 사료 S1, S2 및 S3는 spirulina를 각각 5%, 10% 및 20%를 첨가하여 제조하였다. 또한 P2+S2 사료는 paprika 5%와 spirulina 10%를 혼합하여 제조하였다. 실험종료 후, 쉬리의 생존율은 spirulina가 첨가된 모든 실험구인 S1, S2, S3 및 P2+S2에서 100%로 대조구(80%) 및 paprika 첨가구($73{\sim}93%$)보다 높게 나타났다. 쉬리의 표피 및 지느러미의 총 carotenoids 함량은 색소원이 첨가되지 않은 대조구에서 1.58 mg/100 g으로 가장 낮았으며, spirulina 10% 첨가구인 S2 실험구에서 2.23 mg/100 g으로 가장 높았다. Paprika와 spirulina가 혼합되어 첨가된 실험구인 P2+S2는 1.61 mg/100 g으로 paprika와 spirulina가 각각 첨가된 P2 (2.14 mg/100 g) 및 S2 (2.23 mg/100 g)보다 낮은 총 carotenoids 함량을 보였다. 쉬리의 표피를 육안으로 관찰한 결과 색소원이 첨가된 모든 실험구에서 대조구에 비하여 체색이 더욱 선명화 됨을 관찰 할 수 있었다. 이상의 결과를 고려할 때, 쉬리의 사료내 paprika 첨가시 5%, spirulina 첨가시 10%가 쉬리의 색택개선에 좋을 것으로 판단된다.

• The Korean Journal of Ecology
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• 제28권5호
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• pp.245-254
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• 2005

This paper presents a paleoenvironmental study on Hoya Rincon do Parangueo, a maar lake in Valle de Santiago in Central Mexican Bajio. Maar lake sediments have been widely used for high-resolution reconstruction of paleoenvironment. Many different paleoenvironmental proxy data such as stable isotopes, pollen, sediment chemistry, and dung fungus spore were produced in this study. The pine-oak ratio, stable isotopes, and sediment chemistry help to reveal paleoenviromental changes throughout the whole period covered by sediment materials from this study site. The evidence I found indicates that during ca. 9,500 $\sim$ ca. 8,300 cal yr B.P. there was dry climate; during ca. 8,300 $\sim$ ca. 6,300 cal yr B.P. it was wetter; during ca. 6,300 $\sim$ ca. 4,000 cal yr B.P. drier and cooler; during ca. 4,000 $\sim$ ca. 1,100 cal yr B.P. milder and wetter. The presence of Chupicuaro culture between ca. 2,500 $\sim$ 1,100 cal yr B.P. is implied by the high frequencies of Amaranthaceae and Zea mars. It seems that man left this lake around 1,100 cal yr B.P. due to a dry climate after 1,300 cal yr B.P. Spanish arrival around 400 cal yr B.P. is implied by the fact that fe3 mars reappears and Sporormiella spp. become significant around 120 cm, whereas Poaceae drops sharply.

• Animal Bioscience
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• 제37권7호
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• pp.1204-1212
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• 2024

Objective: This study evaluated the effects of high moisture ear corn (HMEC) on production performance, milk fatty acid composition, serum antioxidant status, and immunity in primiparous dairy cows. Methods: A total of 45 healthy primiparous Holstein cows (36.50±4.30 kg of milk/d, 201±9.00 lactating days in milk) were sorted into 3 groups: control group (CG, n = 15); 50% HMEC (replacing 50% steam-flaked corn with HMEC, n = 15); and 100% HMEC (replacing steam-flaked corn with HMEC, n = 15) on an equal dry matter (DM) basis. The study consisted of adaptation period of 14 days, followed by a formal period of 60 days. Feed intake and milk yield were recorded daily. Milk and blood samples were collected on 1, 30, and 60 d of the experimental period. Results: The 50% HMEC group and 100% HMEC group significantly increased (p<0.05) milk yield and DM intake in dairy cows compared to the control group (CG). The 100% HMEC group showed an increase (p<0.05) in 4% fat-corrected milk (4% FCM). Both the 50% HMEC group and 100% HMEC group exhibited significant decreases (p<0.05) in the content of C10:0, C12:0, and C14:0 fatty acids, along with a significant increase (p<0.05) in cis-9C18:1 content. The saturated fatty acid content was significantly lower (p<0.05) in the 50% HMEC and 100% HMEC groups than that of CG. Conversely, the monounsaturated fatty acid content was higher (p<0.05) in the 50% HMEC and 100% HMEC groups than that in CG. Notably, the 100% HMEC group significantly increased (p<0.05) the serum superoxide dismutase and glutathione peroxidase content, while also decreasing the serum malondialdehyde content (p<0.05). Moreover, the 100% HMEC group significantly increased (p<0.05) the content of immunoglobulin G (IgG) and IgM. Conclusion: High moisture ear corn could improve production performance and milk fatty acid levels and enhance immunity and antioxidant capacity in dairy cows. These results lay the foundation for the wider application of HMEC in ruminant animal diets.

• The Korean Journal of Physiology and Pharmacology
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• 제10권1호
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• pp.7-11
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• 2006

Interstitial cells of Cajal (ICCs) are pacemakers in gastrointestinal tracts, regulating rhythmicity by activating nonselective cation channels (NSCCs). In the present study, we investigated the general characteristics and pH-mediated regulation of pacemaker activity in cultured interstitial cells of Cajal. Under voltage clamp mode and at the holding potential of -60 mV, the I-V relationships and difference current showed that there was no reversal potential and voltage-independent inward current. Also, when the holding potentials were changed from +20 mV to -80 mV with intervals of 20 mV, there was little difference in inward current. In pacemaker activity, the resting membrane potential (RMP) was depolarized (In pH 5.5, $23{\pm}1.5$ mV depolarized) and the amplitude was decreased by a decrease of the extracellular pH. However, in case of increase of extracellular pH, the RMP was slightly hyperpolarized and the amplitude was decreased a little. The melastatin type transient receptor potential (TRPM) channel 7 has been suggested to be required for intestinal pacemaking activity. TRPM7 produced large outward currents and small inward currents by voltage ramps, ranging from +100 to -100 mV from a holding potential of -60 mV. The inward current of TRPM7 was dramatically increased by a decrease in the extracellular pH. At pH 4.0, the average inward current amplitude measured at -100 mV was increased by about 7 fold, compared with the current amplitude at pH 7.4. Changes in the outward current (measured at +100 mV) were much smaller than those of the inward current. These results indicate that the resting membrane potential of pacemaking activity might be depolarized by external acidic pH through TRPM7 that is required for intestinal pacemaking activity.

• 한국산학기술학회논문지
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• 제14권10호
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• pp.5003-5011
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• 2013

본 논문은 정서-주의 태스크를 수행할 때의 신경학적, 행동학적 반응이 정서조절 능력을 반영할 수 있는지를 측정하였다. 이를 위해, 건강한 성인 남녀 19명을 대상으로 3일에 걸쳐 1일 1회씩 동일 시간대에 정서-주의 상호작용이 요구되는 실험을 수행하였다. 이 실험을 통해 정서를 유발하는 비디오를 보여주기 전과 후, 정서-주의 태스크를 수행할 때의 EEG와 반응시간이 측정되었다. ERP P100과 P300 성분 간의 밀접한 상관관계가 나타났으며, P100 진폭이 높을수록 P300 잠재기가 길게 나타났다. 또한 P300 잠재기가 길수록 반응시간이 길게 나타났다. 또한, 정서-주의 태스크를 수행할 때 ERP 성분과 반응시간에 개인별 차이가 존재하였다. 정서유발을 위해 사용된 비디오 자극의 유형에 관계없이 ERP 성분의 낮은 진폭과 빠른 잠재기를 나타낸 집단이 더 빠른 반응시간을 나타내었다. 이러한 반응특성은 목표인식에 대한 성급한 반응으로 해석될 수 있으며, 낮은 정서 조절력과 관련이 있다고 할 수 있다.

• 한국잠사곤충학회지
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• 제17권2호
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• pp.161-170
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• 1975

This study is to investigate the content of crude protein intruded in the sericin of cocoon shell and pupa by treatment of buffer solution (pH 1 to pH 13) 20 ml per 1 gram for 30 and 60 minutes at 30$^{\circ}C$, 60$^{\circ}C$ and 100${\pm}$2$^{\circ}C$, respectively. The results obtained are summarized as follows. 1. The quantity of total crude protein obtained from cocoon shell and pupa by treatment during 30 minutes at 30$^{\circ}C$ was dissolved as the largest quantity of 11.874 mg/g at pH 1 and l5.93mg/g at pH 13, but dissolved the smallest quantity 1.75g/g at pH 5 as known. and tile quantity of crude protein treated for 60 minutes is 13.437mg/g at pH 1 and 22.50mg/g at pH 13. Also, the smallest quantity is 2. 813mg/g at pH 5 as known. 2. By the treatment during 30 minutes at 60$^{\circ}C$, the dissolved largest quantity was 13.12mg/g at pH 1 and 21.875 mg/g at pH 13, but the smallest quantity is 2.375mg/g at pH 5 as known After treatment for 60 minutes at 60$^{\circ}C$, the dissolved largest quantity was 17.500 mg/g at pH 1 and 31.56mg/g at pH 13, bu the smallest quantity is 3.849 mg/g at pH 5. 3. The dissolved crude protein from the cocoon shell and pupa by treatment for 30 minutes at 100${\pm}$2$^{\circ}C$ was the largest quantity of 147.000mg/g at pH 1 and 398. 125mg/g at pH 13, but the smallest quantity is 75.00mg/g at pH 5 as known. After treatment for 60 minutes at 100${\pm}$2$^{\circ}C$, the largest quantity was 253.76 mg/g at pH 1 and 460.625mg/g at pH 13, but the smallest quantity is 139.375mg/g at pH 5 as known. 4. The dissolved crude protein from the cocoon shell and pupa was not different in quantity by treatment at 30$^{\circ}C$ or 60$^{\circ}C$. But dissolved crude protein was large quantity from cocoon shell more than pupa, as known. 5. The treatment of cocoon shell and pupa during 60 minutes at 100${\pm}$20$^{\circ}C$ was increased to the dissolved largest quantity of crude protein of 19.20％ at pH 1 and 22. 18％ at pH 13 from the cocoon shell and 6. 12％ at pH, an d 23.87％ at pH 13 from the pupa. But dissolved crude protein was increased to the larger quantity from pupa more than cocoon shell.