• Title/Summary/Keyword: p-법

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Pathogenicity of Pythium Species Associated with Seed and Seedling Rot in Water-Seeded Rice (벼 담수직파재배에서 벼모썩음병에 관련된 Pythium속 균의 병원성)

  • ;R. W. Schneider
    • Korean Journal Plant Pathology
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    • v.12 no.4
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    • pp.402-406
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    • 1996
  • Pythium 5종, 16균주를 공시하여 담수직파한 벼에 대한 병원성을 CMA 배지와 growth chamber 검정법에 의하여 검정한 결과 P. arrhenomanes 1398 균주가 가장 강한 병원성을 보였으며, 다음은 균주에 따라 차이가 있었으나 P. irregulare와 P. spinosum도 병원성이 강하였으며, P. dissotocum과 P. catenulatum의 균주는 병원성이 약하거나 없엇다. CMA 배지 검정때 P. arrhenomanes 1398 균주의 병원성은 주.dirks 2$0^{\circ}C$ 또는 3$0^{\circ}C$의 항온처리보다 주간 3$0^{\circ}C$, dirks 2$0^{\circ}C$의 변온 처리에서 높았다. Growth chamber 검정법으로 병원성을 검정할 때에는 벼알접종원을 이용하는 것이 효과적이었다.

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Development of a Noble Dosimetry Using Metaphase Analysis and Micronuclei Assay of Bone Marrow Cells in Mice (마우스 골수세포의 중기염색체 분석 및 미소핵 검사를 이용한 피폭선량 평가법의 개발)

  • Min, Jung-Jun;Bom, Hee-Seung;Kim, Young-Ho;Yoon, Hyun-Joong;Kim, Ji-Yeul
    • The Korean Journal of Nuclear Medicine
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    • v.34 no.1
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    • pp.74-81
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    • 2000
  • Purpose: The purpose of this study was to develop in vivo dosimetries using both chromosomal aberrations and micronuclei in mice to assess biological effects of radiations. Materials and Methods: Five each mice were irradiated with 0, 1, 2, 3, 4, 5, 10 Gy of Cs-137 gamma-rays. We scored numbers of chromosomal aberrations in metaphase spreads and numbers of micronuclei in bone marrow smears under light microscope, and obtained the dose-response relationships. We also examined the relationship between the two dose-response curves. Results: The frequency of both chromosomal aberrations and micronuclei increased with dose, in a linear-quadratic manner The delta, beta, and alpha coefficients were 0.0176, 0.0324, and 0.0567 for metaphase analysis (r=1.0, p<0.001) and 0.0019, 0.0073, and 0.0506 for micronuclei assay (r=1.0, p<0.001). The frequency of chromosomal aberrations and micronuclei in different radiation doses was significantly correlated (r=0.99, p<0.01). Conclusion: In vivo dosimetry using either metaphase analysis or micronucleus assay was feasible in mice. These methods could be useful to evaluate biological effects of radiation.

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Determination of Formaldehyde in Cosmetics Using a Convenient DNPH Derivatization Procedure Followed by HPLC Analysis (간편한 DNPH 유도체화 HPLC 분석법을 이용한 화장품 중 포름알데하이드 분석)

  • Choi, Jongkeun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.1
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    • pp.65-73
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    • 2016
  • Korea Food and Drug Administration (KFDA) has officially announced 2,4-dinitrophenylhydrazine (DNPH) derivatization - high performance liquid chromatography (HPLC) methods for analysis of formaldehyde. This study was conducted to develop a convenient derivatization method for cosmetics by improving complex pre-treatment procedures included in KFDA method. To simplify pre-treatment procedures of KFDA method, reaction conditions including pH, time and temperature were optimized. This pre-treatment method does not require complicate pre-treatment steps of KFDA method such as pH adjustment of test solution with acetate buffer (pH 5.0), solvent-solvent partitioning with dichloromethane and concentrating procedure with vacuum evaporator. Formaldehyde-dinitrophenylhydrazone (formaldehyde-DNP) product produced by derivatization reaction was separated and quantified with a reversed-phase HPLC, which was slightly modified with KFDA method. The linearity test showed good results with 0.9999 of correlation coefficient ($r^2$) in the range of 2 ~ 40 ppm of standard solutions. In this method, limit of detection (LOD) and limit of quantitation (LOQ) values for formaldehyde were 0.2 ppm and 0.5 ppm, respectively. In addition, recovery test demonstrated that the method was also accurate and reproducible. Therefore, the proposed method can be applicable to rapid analysis of formaldehyde in cosmetics.

The Effect of Havruta Learning Method on Academic Self-Efficacy, Academic Achievement and Communication Skills of Radiology Students (하브루타 학습법이 방사선과 학생들의 학업적 자기효능감, 학업성취도 및 의사소통능력에 미치는 효과)

  • Gil-Ju Na
    • Journal of the Korean Society of Radiology
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    • v.17 no.6
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    • pp.1009-1015
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    • 2023
  • This study was a one-group pretest-posttest design experimental study that attempted to verify the effects of applying the Havruta learning method on the academic self-efficacy, academic achievement, and communication skills of radiology college students. This study was conducted from May 1 to June 23, 2023, and applied the Havruta learning method for 6 weeks to 38 second-year radiologists taking a radiology technology course at a university in Jeollanam-do. SPSS/WIN 21.0 was used for data analysis, the reliability of the scale was verified, the subject's general characteristics, pre-test values and post-test values for measurement variables were verified with descriptive statistics, and the difference between before and after the Havruta learning method was verified with paired t-test. Research results show that the Havruta learning method improves academic self-efficacy (t=-2.70, p<.001), academic achievement (t=-2.87, p=.006), and communication skills (t=-2.76, p=.008). showed a statistically significant difference. As a result, Havruta learning method was confirmed as an effective learning method that improves academic self-efficacy, academic achievement, and communication skills of radiology college students. In the future, expanded application of the Havruta learning method will be necessary.

Comparison of strip analysis and HPLC analysis for the quantitative analysis of cyanobacterial toxin (남조류 독소 정량을 위한 스트립분석법과 HPLC 분석법의 비교)

  • Pyo, Dongjin;Yim, Miyeon
    • Analytical Science and Technology
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    • v.28 no.3
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    • pp.168-174
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    • 2015
  • Cyanobacterial toxins, such as microcystins, which exist in Korean lakes, are strongly toxic in fish, cattle, and humans. This study performs a quantitative analysis of cyanobacterial toxins in water by comparing the strip method and the HPLC method. Because the detection ranges of the strip method and the HPLC method are different, the water samples were diluted. The comparison of the strip method and the HPLC method was made using seven samples that contained different concentrations of microcystin. The quantitative results produced by the strip analysis were significantly aligned with the results of the HPLC analysis. The results of correlation analysis were r = 0.99998 and p = 0.00001.

Minutes of ICSH Panel Meeting Held in the Palace Hotel Noordwijk, Netherlands from 18th-23rd November, 1973

  • Belcher E.H.;Eernisse J.G.;Glass H.I.;Heimpel H.;Lewis S.M.;Mollison P.;Murphy A.E.;Naiean Y.;Szur L.;Dormer I.;Ganzoni A.;Koning J. De;Scheer K.E.
    • The Korean Journal of Nuclear Medicine
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    • v.8 no.1_2
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    • pp.63-70
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    • 1974
  • 이 논문(論文)은 1973년(年) ICSH주최로 옅린 panel에서 혈소판수명(血小板壽命) 측정법(測定法)의 표준화(標準化)에 관(關)한 토론결과(討論結果)를 기록(技錄)한 것이다. 이 prnel에서는 주(主)로 혈소판수명측정(血小板壽命測定)의 기술적(技術的)인 면(面)과 분석적(分析的)인 면(面)을 토론(討論)하고 있다. 현소판(血小板)에 방사성(放射性) 물질(物質)의 표지법(標識法)으로는 "Cohor"법(法)과 "Random"표지법(標識法)이 있으며 전자(前者)는 분석적(分析的)인 면(面)에서는 "Random" 표지법(標識法) 보다 좋으나 현재로서는 표지법(標識法)으로서 만족하지는 않다. "Random"표지법(標識法)으로는 $^{14}C$-serotonime, DEP, $^{32}P,\;^3H$$^{51}Cr$ 등(等)이 이용(利用)되고 있다. DEP는 현재 널리 사용되고 있으나 DEP는 주입후(注入後) 2주(週)까지 방사능(放射能)이 처음의 $10{\sim}15%$정도(程度)가 계속 남아있고 또한 혈소판이외(血小板以外)에도 백혈구(白血球), 적혈구등(赤血球等)에도 상당히 많이 표지(標識)되므로 혈소판(血小板)만 따로 분리(分離)해야 되는 단점이 있어서 이 panel에서는 사용하지 않는 것이 좋다고 하였다. 반면 $^{51}Cr$은 기술적(技術的)인 문제(問題)가 이미 많이 해결되어 있어 추천하고 있다. 여기서는 혈소판수명(血小板壽命) 측정법(測定法)에서 혈소판분리(血小板分離) 및 표지(標識), 채혈(採血) 및 계측(計測), data 분석법등(分析法等)에 관(關)하여 논(論)하고 있다.

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Fatigue Crack Propagation Analysis by P-Version of Finite Element Method (P-version 유한요소법에 의한 피로균열해석)

  • 우광성;이채규
    • Computational Structural Engineering
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    • v.5 no.3
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    • pp.97-103
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    • 1992
  • Since many design problems in the railroad, aerospace and machine structures involve considerations of the effect of cyclic loading, manufacturing and quality control processes much fully account for fatigue of critical components. Due to the sensitivity of the Paris law, it is very important to calculate .DELTA.K numerically to minimize the error of predicted fatigue life in cycles. However, it is shown that the p-version of FEM based on LEFM analysis is far better suited for computing the stress intensity factors than the conventional h-version. To demonstrate the proficiency of the proposed scheme, the welded T-joint with crack problems of box car body bolster assembly and a crack problem emanating from a circular hole in finite strip have been solved.

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Suspension polymerized toner prepared using hydroxyapatite as a suspending agent (하이드록시아파타이트를 분산제로 사용하여 제조한 현탁중합 토너)

  • Yu, Jae-Goang;Kim, Duk-Eung;Park, Sol-Mon;Kim, Dae-Su
    • Proceedings of the KAIS Fall Conference
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    • 2010.11a
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    • pp.474-477
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    • 2010
  • 최근 디지털 장비의 발전에 발맞추어 고속, 고화질의 컬러 문서가 요구됨에 따라 레이저 프린터의 보급 또한 급속도로 성장하고 있는 실정이다. 토너는 고속, 고화질의 컬러 문서 인쇄에 가장 중요한 역할을 하는 것으로, 크게 분쇄법과 중합법에 의해 제조 되고 있다. 그러나 그 중 분쇄법 토너는 근본적으로 해결되지 않는 문제점들이 있다. 즉, 중합된 수지를 사용함으로 인한 단가상승과 수지와 내첨제를 용융혼합하는 과정에서의 분산의 한계성, 그리고 인쇄 품질의 좋지 않은 것이 그 대표적인 예이다. 이에 본 실험에서는 고속, 고화질의 컬러 프린터에 적합한 중합법에 의한 토너 제조시 하이드록시아파타이트가 토너 제조에 미치는 영향에 대해서 연구하였다. Ca/P의 몰비에 따라 제조된 하이드록시아파타이트가 토너의 입자형성에 크게 영향을 주었으며, 이에 따라 토너의 물성에도 영향을 주는 것으로 나타났다. 또한 pH에 따라서도 토너중합에 많은 영향을 주는 것으로 나타났다. 토너 입자 형성에 적절한 Ca/P의 몰비 및 pH가 존재 하며, 이를 최적으로 조절하면 양질의 토너를 제조 할 수 있을 것으로 판단된다.

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Background impurity incorporation in the growth of InP by hydride vapor phase epitaxy technique (Hydride 기상증착법을 이용한 InP 성장에서의 배경 불순물 도입에 관한 연구)

  • Chinho Park
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.6 no.2
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    • pp.141-154
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    • 1996
  • Intrinsic layers of homoepitaxial InP grown by the hydride vapor phase epitaxy (VPE) technique were investigated by Fourier-transform photoluminescence(FTPL) and variable temperature Hall measurements. The effect of process variables (i.e., source zone temperature and inlet mole fractions of HCl and $PH_{3}$) on the backgroudn impurity levels was investigated. The background carrier concentration was found to decrease with decreasing source zone temperature and increasing HCl, but was relatively independent of $PH_{3}$ for the range of mole fraction studied. The presence of background donors and acceptors was clearly verified in the FTPL spectra, and the major impurities were tentatively identified as Si donors and Zn acceptors as well as some unidentified acceptors.

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Determination of Chloramphenicol by Differential Pulse Polarography (미분 펄스 폴라로그래피에 의한 Chloramphenicol의 정량분석)

  • Younghee Hahn;Jung-Sun Jeon
    • Journal of the Korean Chemical Society
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    • v.36 no.4
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    • pp.552-557
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    • 1992
  • Chloramphenicol was studied by differential pulse polarography (DDP). A reduction peak which is dependent on pH of the solution appeared in the voltage range between zero and -1.50 volt vs. Ag/AgCl (sat. KCl) reference electrode. A plot of peak potentials (Ep) measured at room temperature (20$^{\circ}C$) vs. pH of the chloramphenicol solutions showed linear relationship changing slope (Ep/pH) at pH 8.9. The slope was -59.7 mV/pH in pH 2.7∼8.9 and -24.3 mV/pH in pH 8.9∼11.2, respectively. A log plot of peak currents (ip) vs. concentrations showed a linearity at the concentrations between 4.8 ${\times}$ 10$^{-7}$ M and 6.2 ${\times}$ 10$^{-5}$ M (0.16 ppm∼20 ppm) chloramphenicol in pH 8.0 ammonium buffer. Between the DPP method and the reference method measuring absorbance at 278 nm, the correlation coefficient was 0.996, which means an excellent linearity. The DPP method was able to detect degradation products of chloramphenicol in mild alkaline solution (pH = 8.0) more distinctly than the spectrophotometric method.

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