• Title/Summary/Keyword: oxygen transfer

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Animal Cell Culture and the Production of Monoclonal Antibody(MAb) Using Biopolymer Membrane (생물고분자 막 형성을 이용한 동물세포 배양 및 단클론항체 생산)

  • 손정화;유선희;김성구
    • KSBB Journal
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    • v.13 no.1
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    • pp.13-19
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    • 1998
  • Biopolymer membrane was prepared using two oppositely charged natural biopolymers. The biopolymer membrane was used for the encapsulation of two hybridoma cell lines(ATCC CRL-1606, ATCC HB-8852) to produce monoclonal antibodies. In order to reduce the down stream steps, the pre size of the membrane was controlled to retain the monoclonal antibodies in the capsules based on the diffusion experiments with standard proteins. T-flask culture showed cell densities of 8$\times$107 cells/mL and 3$\times$107 cells/mL, and MAb concentrations of 506$\mu$g/mL and 109$\mu$g/mL for encapsulated ATCC CRL-1606 and HB-8852, respectively. Two liter perfusion cultures with encapsulated ATCC HB-8852 were performed to enhance the MAb production. The MAb production of the encapsulated hybridoma increased considerably comparing to the culture using silicon tubing for oxygen transfer.

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The Possibility and Risk of Generation of Cavitation at the inlet of the Turbopump (선화제펌프 입구에서 캐비테이션 발생 가능성 및 위험성 평가)

  • Kim, Cheul-Woong;Moon, In-Sang;Bershadskiy, V.A
    • 유체기계공업학회:학술대회논문집
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    • 2006.08a
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    • pp.279-282
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    • 2006
  • Upon a turbopump's running, cavitation may occur at the inlet of the LOx pump by pressure drop and heat transfer along the LOx feeding line. Since the cavitation can cause serious damage to the pump or to stop running, the absence of the cavitation at the inlet of a turbopump should be confirmed before the using the turbopump. In the present study, the calculation of the volume fraction of LOx gas phase at the inlet of the pump are performed with different temperatures of LOx in the tank, pressure drops and heat transfers along the feeding line. This calculation method can be applied to define the limits of thermal and hydraulic characteristics during the design of a LOx feeding system.

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Effect of Surface Modificaion on the Rheology and Property of CNTs/Epoxy Nanocomposites (표면 개질된 탄소나노튜브/에폭시 나노복합재료의 유변학적 거동과 물성)

  • Kim Jin Ah;Seong Dong Gi;Youn Jae Ryoun
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2004.10a
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    • pp.43-46
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    • 2004
  • Multi-walled carbon nanotubes (MWNTs) produced by chemical vapor deposition were treated with acidic solution for purification and oxidization of CNTs. The surface modification of the oxidized CNTs was achieved by amine treatment and oxygen plasma treatment. The functionalized CNTs were embedded in the epoxy resin by sonication method and the resulting composite was investigated by FESEM. Rheological and mechanical properties of nanocomposites were measured by AR2000 and Instron. The rheological properties and dispersion of modified CNTs/epoxy composites were improved as CNTs were modified, because the modification of CNTs led to a improvement interaction between the CNTs and the epoxy resin. In addition to this, mechanical properties are also improved because of the effective stress transfer between the CNTs and the polymer.

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Preparation of Porous PLGA Microfibers Using Gelatin Porogen Based on a Glass Capillary Device (젤라틴 기공유도물질과 유리모세관 장치를 이용한 다공성 PLGA 미세섬유의 제조)

  • Kim, Chul Min;Kim, Gyu Man
    • Journal of the Korean Society for Precision Engineering
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    • v.33 no.1
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    • pp.63-67
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    • 2016
  • We present a method of fabricating poly (lactic-co-glycolic acid) (PLGA) porous microfibers using a pore template. PLGA microfibers were synthesized using a glass capillary tube in a poly-(dimethylsiloxane) (PDMS) microfluidic chip. Gelatin solution was used as a porous template to prepare pores in microfibers. Two phases of PLGA solutions in different solvents-DMSO (dimethyl sulfoxide) and DCM (dichloromethane)-were used to control the porosity and strength of the porous microfibers. The porosity of the PLGA microfibers differed depending on the ratio of flow rates in the two phases. The porous structure was formed in a spiral shape on the microfiber. The porous structure of the microfiber is expected to improve transfer of oxygen and nutrients, which is important for cell viability in tissue engineering.

The Study of Preparation of Dioxygen Bridged Palladium Complexes Having Amine Ligands (아민을 리간드로 갖는 산소가교 팔라듐 착화합물의 합성에 관한 연구)

  • Chung, Pyung-Jin
    • Applied Chemistry for Engineering
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    • v.3 no.1
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    • pp.64-71
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    • 1992
  • New type dioxygen bridged complexes of palladium were prepared by using $KO_2$ as a source of superoxide ion($O_2{^-}$). The method is completely different from the traditional one which has adopted the oxidative addition of molecular oxygen to prepare the dioxygen complexes. This reaction proceeds via nucleophilic displacement followed by electron transfer reaction. Three new type dioxygen complexes of palladium having amine ligands were prepared and characterized by the application of the reaction of $O_2{^-}$.

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Study of the breathable film processing techniques for microwave oven using a laser (레이저를 이용한 전자렌지용 숨쉬는 필름 가공 기술 연구)

  • Sohn, Ik-Bu;Choi, Hun-Kook;Yoo, Dong-Yoon;Noh, Young-Chul;Kim, Joung-Nyon;Kang, Ho-Min
    • Laser Solutions
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    • v.16 no.2
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    • pp.16-18
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    • 2013
  • In this paper, we fabricated breathable films on the use of microwave oven by using UV nanosecond laser micromachining, and the number of micro-grooves on the film is controlled for different oxygen transfer rate(OTR). As different number of micro-groove, the breath films of 100,000cc, 120,000cc, and 150,000cc can be fabricated. The breath film package of 120,000cc is used for the experiment of steaming a sweet potato. At the result, the sweet potato is well-cooked with enough moisture in the package not bursted.

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Ammonia Decomposition Over Tantalum Carbides of Hydrogen Fuel Cell (수소연료전지용 탄탈륨 탄화물에 대한 암모니아 분해반응)

  • Choi, Jeong-Gil
    • New & Renewable Energy
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    • v.9 no.1
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    • pp.51-59
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    • 2013
  • Tantalum carbide crystallites which is to be used for $H_2$ fuel cell has been synthesized via a temperature-programmed reduction of $Ta_2O_5$ with pure $CH_4$. The resultant Ta carbide crystallites prepared using two different heating rates and space velocity exhibit the different surface areas. The $O_2$ uptake has a linear relation with surface area, corresponding to an oxygen capacity of $1.36{\times}10^{13}\;O\;cm^{-2}$. Tantalum carbide crystallites are very active for hydrogen production form ammonia decomposition reaction. Tantalum carbides are as much as two orders of magnitude more active than Pt/C catalyst (Engelhard). The highest activity has been observed at a ratio of $C_1/Ta^{{\delta}+}=0.85$, suggesting the presence of electron transfer between metals and carbon in metal carbides.

Application of mid-infrared TDLAS to various small molecule diagnostics

  • Lee, Young-Sik
    • The Bulletin of The Korean Astronomical Society
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    • v.35 no.1
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    • pp.25-25
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    • 2010
  • The spectroscopy over a region from 3 to 17 ${\mu}m$ based on the tuneable diode lasers (TDLAS) is the most powerful technique for in situ studies of the diagnostics of small molecules. The increasing interest in small molecules especially containing carbon, oxygen, hydrogen, and fluorine containing ones can be fulfilled by TDLAS at 0.0001 cm-1 resolution, because most of these compounds are infrared active. TDLAS provides a means of determining the absolute concentrations of the ground states of stable and transient molecular species, which can be employed for the time dependent studies in sub micro second scale. Information about gas temperature and population densities can also be derived from TDLAS measurements. Collisional energy transfer between the small molecules can be studied with TDLAS. Also, a variety of free radicals and molecular ions have been detected by TDLAS. Since plasmas with molecular feed gases are used in many applications, there are new applications in industrial field. Recently, the development of quantum cascade lasers (QCLs) offers an attractive new option for TDLAS.

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Rigorous Model for Spherical Cell-support Aggregate

  • Moon, Seung-Hyeon;Lee, Ki-Beom;Satish J. Paruekar
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.1
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    • pp.42-50
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    • 2001
  • The activity of immobilized cell-support particle aggregates is influenced by physical and biochemical elements, mass transfer, and physiology. Accordingly, the mathematical model discussed in this study is capable of predicting the steady state and transient concentration profiles of the cell mass and substrate, plus the effects of the substrate and product inhibition in an immobilized cell-support aggregate. The overall mathematical model is comprised of material balance equations for the cell mass, major carbon source, dissolved oxygen, and non-biomass products in a bulk suspension along with a single particle model. A smaller bead size and higher substrate concentration at the surface of the particle, resulted in a higher supply of the substrate into the aggregate and consequently a higher biocatalyst activity.

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New Red Phosphor with the Improved Color Purity for PDP Applications

  • Mho, Sun-Il
    • 한국정보디스플레이학회:학술대회논문집
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    • 2002.08a
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    • pp.257-259
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    • 2002
  • As a new host material for a red phosphor for PDP applications, has studied (Y,Gd)$Al_3(BO_3)_4$ which gives non-centrosymmetric sites for $Eu^{3+}$ activators. Vacuum ultraviolet (VUV) excitation spectrum of new red phosphor (Y,Gd)$Al_3(BO_3)_4$:$Eu^{3+}$ has two broad bands. One band with the absorption edge at ca. 168 nm is the band-gap absorption of aluminoborate and the other broad band centered 240 nm is the charge transfer transition between $Eu^{3+}$ and the neighboring oxygen anions. The PL spectrum shows the strongest emission at 617 nm due to the electric dipole $^5D_0{\rightarrow}^7F_2$ transition of $Eu^{3+}$, whose luminescent chromaticity is (0.67, 0.33).

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